scholarly journals Plasma Carboxyl-Metabolome Is Associated with Average Daily Gain Divergence in Beef Steers

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 67
Author(s):  
Ibukun Ogunade ◽  
Adeoye Oyebade ◽  
Bremansu Osa-Andrews ◽  
Sunday Peters
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Isotope Labeling ◽  
Ketone Bodies ◽  
Average Daily Gain ◽  
Acid Oxidation ◽  
Beef Steers ◽  
Volcano Plot ◽  
Daily Gain ◽  
Chain Fatty Acids

We applied an untargeted metabolomics technique to analyze the plasma carboxyl-metabolome of beef steers with divergent average daily gain (ADG). Forty-eight newly weaned Angus crossbred beef steers were fed the same total mixed ration ad libitum for 42 days. On day 42, the steers were divided into two groups of lowest (LF: n = 8) and highest ADG (HF: n = 8), and blood samples were obtained from the two groups for plasma preparation. Relative quantification of carboxylic-acid-containing metabolites in the plasma samples was determined using a metabolomics technique based on chemical isotope labeling liquid chromatography mass spectrometry. Metabolites that differed (fold change (FC) ≥ 1.2 or ≤ 0.83 and FDR ≤ 0.05) between LF and HF were identified using a volcano plot. Metabolite set enrichment analysis (MSEA) of the differential metabolites was done to determine the metabolic pathways or enzymes that were potentially altered. In total, 328 metabolites were identified. Volcano plot analysis revealed 43 differentially abundant metabolites; several short chain fatty acids and ketone bodies had greater abundance in HF steers. Conversely, several long chain fatty acids were greater in LF steers. Five enzymatic pathways, such as fatty acyl CoA elongation and fatty-acid CoA ligase were altered based on MSEA. This study demonstrated that beef steers with divergent ADG had altered plasma carboxyl-metabolome, which is possibly caused by altered abundances and/or activities of enzymes involved in fatty acid oxidation and biosynthesis in the liver.

scholarly journals PSII-2 Beef steers with divergent average daily gain have altered plasma amine/phenol-metabolome

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 399-399
Author(s):  
Ibukun M Ogunade
Keyword(s):  
Isotope Labeling ◽  
Average Daily Gain ◽  
Plasma Concentrations ◽  
Plasma Metabolites ◽  
Free Access ◽  
Beef Steers ◽  
Amino Acid Residues ◽  
Branched Chain ◽  
Chemical Groups ◽  
Daily Gain

Abstract This study applied a chemical isotope labeling/liquid chromatography mass spectrometry-based metabolomics technique to determine differences in plasma metabolites containing amine/phenol and carbonyl chemical groups in beef steers with divergent average daily gain (ADG). Thirty-eight Angus crossbred beef steers (21 d post-weaning; 210 ± 12 kg of BW) from a single source were housed in individual slatted floor pens and were fed the same total mixed ration (CP = 14.5% and NEg = 1.10 Mcal/kg) ad libitum for 42 d with free access to water. After 42 days of feeding, the steers were divided into two groups of lowest ADG (LF: n = 8) and highest (HF: n = 8) ADG. Blood samples were taken from both LF and HF steers and were immediately centrifuged to harvest the plasma. The average daily DM intake of the steers in LF and HF were 6.08 kg ± 0.57 and 6.04 kg ± 0.42, respectively, and was similar between the two groups (P = 0.72). The ADG of LF (0.99 kg ± 0.23) was lower (P = 0.01) than that of HF (1.63 kg ± 0.20). A total number of 42 carbonyl-containing metabolites and 229 amine/phenol-containing metabolites were identified in the plasma samples of both groups. No alteration in carbonyl-metabolome was detected. Ten metabolites including 4,6-dihydroxyquinoline, prolyl-valine, prolyl-leucine, prolyl-isoleucine, L-formylkynurenine, pyrocatechol, and histidine were greater in HF steers whereas 8 metabolites including arginine, phenylalanine, guanidoacetic acid, and aspartyl-threonine were greater in LF steers. This study demonstrated that beef steers with divergent ADG had altered plasma amine/phenol metabolome. Notably, plasma concentrations of dipeptides containing branched chain amino acid residues (prolyl-valine, prolyl-leucine, prolyl-isoleucine) and metabolites with anti-inflammatory and reactive oxygen-scavenging properties (4,6-dihydroxyquinoline and L-formylkynurenine) were greater in steers with high ADG.

Meal composition affects postprandial fatty acid oxidation

1993 ◽  
Vol 264 (6) ◽  
pp. R1065-R1070 ◽  
Author(s):  
D. M. Surina ◽  
W. Langhans ◽  
R. Pauli ◽  
C. Wenk
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Nutrient Utilization ◽  
Whole Body ◽  
Acid Oxidation ◽  
Plasma Ffa ◽  
Hepatic Fatty Acid Oxidation ◽  
Beta Hydroxybutyrate ◽  
Chain Fatty Acids

The influence of macronutrient content of a meal on postprandial fatty acid oxidation was investigated in 13 Caucasian males after consumption of a high-fat (HF) breakfast (33% carbohydrate, 52% fat, 15% protein) and after an equicaloric high-carbohydrate (HC) breakfast (78% carbohydrate, 6% fat, 15% protein). The HF breakfast contained short- and medium-chain fatty acids, as well as long-chain fatty acids. Respiratory quotient (RQ) and plasma beta-hydroxybutyrate (BHB) were measured during the 3 h after the meal as indicators of whole body substrate oxidation and hepatic fatty acid oxidation, respectively. Plasma levels of free fatty acids (FFA), triglycerides, glucose, insulin, and lactate were also determined because of their relationship to nutrient utilization. RQ was significantly lower and plasma BHB was higher after the HF breakfast than after the HC breakfast, implying that more fat is burned in general and specifically in the liver after an HF meal. As expected, plasma FFA and triglycerides were higher after the HF meal, and insulin and lactate were higher after the HC meal. In sum, oxidation of ingested fat occurred in response to a single HF meal.

scholarly journals Development of fatty acid oxidation in neonatal guinea-pig liver

1982 ◽  
Vol 208 (3) ◽  
pp. 723-730 ◽  
Author(s):  
D A Shipp ◽  
M Parameswaran ◽  
I J Arinze
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Guinea Pig ◽  
Ketone Bodies ◽  
Liver Mitochondria ◽  
Fatty Acyl ◽  
Acid Oxidation ◽  
Beta Oxidation ◽  
Pig Liver ◽  
Guinea Pig Liver

The capacity of foetal and neonatal liver to oxidize short-, medium- and long-chain fatty acids was studied in the guinea pig. Liver mitochondria from foetal and newborn animals were unable to synthesize ketone bodies from octanoate, but octanoylcarnitine and palmitoylcarnitine were readily ketogenic. The ketogenic capacity at 24 h after birth was as high as in adult animals. Hepatocytes isolated from term animals were unable to oxidize fatty acids, but at 6 h after birth production of 14CO2, acid-soluble products and acetoacetate from 1-14C-labelled fatty acids was 40-50% of the rates at 24 h. At 12 h of age these rates had already reached the 24 h values and did not change during suckling in the first week of life. The activities of hepatic fatty acyl-CoA synthetases, which were minimal in the foetus or at term, increased to maximal values in 12-24 h. The data show that the capacity for beta-oxidation and ketogenesis develops maximally in this species during the first 6-12 h after birth, and appears to be partly dependent on the development of fatty acid-activating enzyme.

scholarly journals Drug triggered pruritus, rash, papules, and blisters – is AGEP a clash of an altered sphingolipid-metabolism and lysosomotropism of drugs accumulating in the skin?

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Markus Blaess ◽  
Lars Kaiser ◽  
Oliver Sommerfeld ◽  
René Csuk ◽  
Hans-Peter Deigner
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Uv Radiation ◽  
Sphingolipid Metabolism ◽  
Acid Oxidation ◽  
Long Chain Fatty Acids ◽  
Mitochondrial Fatty Acid ◽  
The Individual ◽  
Long Chain ◽  
Chain Fatty Acids

AbstractRash, photosensitivity, erythema multiforme, and the acute generalized exanthematous pustulosis (AGEP) are relatively uncommon adverse reactions of drugs. To date, the etiology is not well understood and individual susceptibility still remains unknown. Amiodarone, chlorpromazine, amitriptyline, and trimipramine are classified lysosomotropic as well as photosensitizing, however, they fail to trigger rash and pruritic papules in all individuals. Lysosomotropism is a common charcteristic of various drugs, but independent of individuals. There is evidence that the individual ability to respond to external oxidative stress is crosslinked with the elongation of long-chain fatty acids to very long-chain fatty acids by ELOVLs. ELOVL6 and ELOVL7 are sensitive to ROS induced depletion of cellular NADPH and insufficient regeneration via the pentose phosphate pathway and mitochondrial fatty acid oxidation. Deficiency of NADPH in presence of lysosomotropic drugs promotes the synthesis of C16-ceramide in lysosomes and may contribute to emerging pruritic papules of AGEP. However, independently from a lysosomomotropic drug, severe depletion of ATP and NAD(P)H, e.g., by UV radiation or a potent photosensitizer can trigger likewise the collapse of the lysosomal transmembrane proton gradient resulting in lysosomal C16-ceramide synthesis and pruritic papules. This kind of papules are equally present in polymorphous light eruption (PMLE/PLE) and acne aestivalis (Mallorca acne). The suggested model of a compartmentalized ceramide metabolism provides a more sophisticated explanation of cutaneous drug adverse effects and the individual sensitivity to UV radiation. Parameters such as pKa and ClogP of the triggering drug, cutaneous fatty acid profile, and ceramide profile enables new concepts in risk assessment and scoring of AGEP as well as prophylaxis outcome.

Effects of soybean meal versus processed whole soybean diets on the performance of young bulls and the fatty acid composition of intramuscular fat

2020 ◽  
Vol 60 (11) ◽  
pp. 1435
Author(s):  
C. V. R. Oliveira ◽  
J. P. Schoonmaker ◽  
D. R. Casagrande ◽  
O. R. Machado Neto ◽  
V. A. A. Reis ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Soybean Meal ◽  
Feed Efficiency ◽  
Unsaturated Fatty Acids ◽  
Average Daily Gain ◽  
Carcass Traits ◽  
Ether Extract ◽  
Ground Soybean ◽  
Daily Gain

Context It is hypothesised that the use of processed soybean for feedlot beef cattle improves feed efficiency and produces beef with a better fatty acid profile for human health. Aims This study aimed to evaluate average daily gain, feed efficiency, carcass traits, chemical composition, fatty acid profile and colour in the beef of young bulls fed diets with ground or extruded soybean. Methods A total of 60 young Zebu bulls (Nellore or Nellore crossed with other Zebu breeds) with an average initial liveweight of 320 ± 8.12 kg and an average initial age of 20 ± 2 months were randomly assigned to receive one of the following diets for 84 days: dehulled and defatted soybean meal (3.22% of ether extract), ground soybean (6.51% of ether extract) or extruded soybean (6.37% of ether extract). The fatty acid profiles of these animals were analysed using high-resolution gas chromatography. The CIE L*a*b* colour space model was used to numerically describe the colour during the aging period (0, 7, 14 and 21 days). Key results Diet had no effect on the average daily gain, feed efficiency or carcass traits of the animals (P > 0.05). Protein, ether extract and ash composition of the Longissimus lumborum (LL) muscle were not affected (P > 0.30) by the use of processed soybean grains. The ground soybean diet decreased oleic acid and C18:2 c9, t11 concentrations, but increased C18:2 t10, c12 and trans-octadecenoic acid isomer concentrations in the LL muscle compared with those in the dehulled and defatted soybean meal and extruded soybean diets (P < 0.05). Muscle from bulls fed processed soybean exhibited greater concentrations of stearic acid and saturated fatty acids, and a lower concentration of unsaturated fatty acids, as well as a decreased unsaturated fatty acids:saturated fatty acids ratio (P < 0.05). Processed soybean grains did not affect (P > 0.05) the LL muscle pH or colour. Conclusions The use of ground or extruded soybean did not affect the performance, carcass traits, LL protein, ether extract or ash composition, and had no impact on beef colour compared with the diet containing soybean meal. Processed whole soybeans in the diet did not increase unsaturated fatty acids or conjugated linoleic acid in beef compared with a diet without soybean meal. Implications Up to 20% of ground or extruded soybean in feedlot beef cattle can be used as a replacement for soybean meal and corn.

Effect of full-fat hemp seed on performance and tissue fatty acids of feedlot cattle

2005 ◽  
Vol 85 (2) ◽  
pp. 223-230 ◽  
Author(s):  
D. J. Gibb ◽  
M. A. Shah ◽  
P. S. Mir ◽  
T. A. McAllister
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Feed Efficiency ◽  
Detrimental Effect ◽  
Average Daily Gain ◽  
Feedlot Cattle ◽  
Fatty Acid Profiles ◽  
Hemp Seed ◽  
Finishing Diets ◽  
Daily Gain

Sixty individually penned steers (380 ± 39 kg) were fed barley-based finishing diets containing 0 (control), 9 or 14% full-fat hemp seed (HS) and effects on performance and tissue fatty acid profiles were assessed. At harvest, samples of pars costalis diaphragmatis (PCD) and brisket fat were collected from each carcass. Feeding HS did not affect (P > 0.25) dry matter intake (DMI), average daily gain (ADG), or gain feed-1. Carcass traits were also unaffected (P > 0.35) by treatment. Feeding HS linearly increased (P < 0.001) proportions of C18:0, C18:3 and C18:1 trans-9 in PCD, and 18:2 trans, trans in both PCD and brisket fat. As well, HS linearly increased cis-9 trans-11 CLA (P < 0.001), total saturates (P = 0.002) and polyunsaturated fatty acids (PUFA) (P = 0.01) in PCD. The presence of C20:4, C20:5 and C22:5 was detected only in tissues of cattle supplemented with HS (P < 0.06). Linear reductions (P < 0.002) in C16:1 cis, C17:1, C18:1 cis -9, C20:1, and total unsaturates in PCD, as well as linear decreases in C17:0 (P = 0.04) and C17:1 (P < 0.001) in brisket fat were observed when HS was fed. Levels of HS up to 14% of diet ary DM exerted no detrimental effect on the growth or feed efficiency of cattle as compared to cattle fed a standard barley-based finishing diet. Including HS in the diet had both positive (increased CLA content) and negative (increased trans and saturated fats) effects on fatty acid profiles of beef tissues. Key words: Beef, conjugated linoleic acid, full-fat oilseed, hemp seed, tissue fatty acids

scholarly journals Hepatic Peroxisomal Fatty Acid β-Oxidation Is Regulated by Liver X Receptor α

Endocrinology ◽  
2005 ◽  
Vol 146 (12) ◽  
pp. 5380-5387 ◽  
Author(s):  
Tonghuan Hu ◽  
Patricia Foxworthy ◽  
Angela Siesky ◽  
James V. Ficorilli ◽  
Hong Gao ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Liver Steatosis ◽  
Liver X Receptor ◽  
Acid Oxidation ◽  
Peroxisome Proliferator ◽  
Long Chain Fatty Acids ◽  
Lxr Agonists ◽  
Long Chain ◽  
Chain Fatty Acids

Peroxisomes are the exclusive site for the β-oxidation of very-long-chain fatty acids of more than 20 carbons in length (VLCFAs). Although the bulk of dietary long-chain fatty acids are oxidized in the mitochondria, VLCFAs cannot be catabolized in mitochondria and must be shortened first by peroxisomal β-oxidation. The regulation of peroxisomal, mitochondrial, and microsomal fatty acid oxidation systems in liver is mediated principally by peroxisome proliferator-activated receptor α (PPARα). In this study we provide evidence that the liver X receptor (LXR) regulates the expression of the genetic program for peroxisomal β-oxidation in liver. The genes encoding the three enzymes of the classic peroxisomal β-oxidation cycle, acyl-coenzyme A (acyl-CoA) oxidase, enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase, and 3-ketoacyl-CoA thiolase, are activated by the LXR ligand, T0901317. Accordingly, administration of T0901317 in mice promoted a dose-dependent and greater than 2-fold increase in the rate of peroxisomal β-oxidation in the liver. The LXR effect is independent of PPARα, because T0901317-induced peroxisomal β-oxidation in the liver of PPARα-null mice. Interestingly, T0901317-induced peroxisomal β-oxidation is dependent on the LXRα isoform, but not the LXRβ isoform. We propose that induction of peroxisomal β-oxidation by LXR agonists may serve as a counterregulatory mechanism for responding to the hypertriglyceridemia and liver steatosis that is promoted by potent LXR agonists in vivo; however, additional studies are warranted.

scholarly journals Productive and reproductive performance and blood chemistry on grazing Brahman replacement heifers supplemented with fatty acids and protein

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
José Luis Bello Faria ◽  
Robert Emilio Mora Luna ◽  
Ana María Herrera-Angulo ◽  
Bladimiro Acosta-Rivas
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Average Daily Gain ◽  
Blood Chemistry ◽  
Protein Supplementation ◽  
Long Chain Fatty Acids ◽  
Calcium Salts ◽  
Daily Gain ◽  
Long Chain ◽  
Chain Fatty Acids

To evaluate the effect of calcium salts of long-chain fatty acids (CaLCFA) and protein supplementation on body weight (BW), average daily gain (ADG), pregnancy rate (PR), and blood chemistry, 57 Brahman heifers with 309.1 ± 3.04 kg BW and 1,010 ± 8.51 days of age, were divided into three BW uniform groups and assigned for 102 days to three treatments under a completely randomized design: 1) grazing forage only (F); 2) F+CaLCFA, and 3) F+CaLCFA+protein-energy mix (CaLCFA+CP) with 453 protein g/kg, containing mainly (g/kg) CaLCFA (200), hydrolyzed feather meal (300), and cornmeal (375). About 0.2 and 1 kg/animal/d of CaLCFA and CaLCFA+CP were offered, respectively, with an average intake of 200 and 192 g/animal/d for the same order of supplements. The breeding period (BP) lasted for 45 days and started 57 days after beginning the supplementation period. Body weight showed a treatment × time interaction (p < 0.01), being different at the end of the BP (348; 338 and 331 kg, respectively for CaLCFA, CaLCFA+CP, and F). Average daily gain (g/d) was higher (p < 0.01) for CaLCFA (358) than for CaLCFA+CP (281), and lower for F (206). Overall, PR was higher (p < 0.05) for CaLCFA+CP (68.4 %) and lower for F (36.8 %). Cholesterol (mg/dL) was higher (p < 0.05) for CaLCFA+CP (139) and lower for F (117). Calcium salts of long-chain fatty acids and CaLCFA+CP supplementation improved BW and ADG of Brahman heifers at first mating, while PR and cholesterol increased only with CaLCFA+CP supplementation.

Whole body energy expenditure and fuel oxidation after 2,5-anhydro-D-mannitol administration

1995 ◽  
Vol 268 (1) ◽  
pp. R299-R302 ◽  
Author(s):  
C. R. Park ◽  
R. J. Seeley ◽  
L. Benthem ◽  
M. I. Friedman ◽  
S. C. Woods
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Food Intake ◽  
Ketone Bodies ◽  
Whole Body ◽  
Acid Oxidation ◽  
Metabolic Variables ◽  
Continued Use ◽  
Body Energy ◽  
Lines Of Evidence

The fructose analogue 2,5-anhydro-D-mannitol (2,5-AM) increases food intake in nondeprived rats. Several lines of evidence indicate that vagal signals arising from the liver are critical for this effect. In addition, 2,5-AM decreases plasma glucose and increases lipolysis, resulting in an increase in plasma free fatty acids and ketone bodies. In these respects 2,5-AM produces a state analogous to that observed after food deprivation. Using an indirect calorimeter, we determined that 2,5-AM (300 mg/kg ip) causes a potent and long-lasting decrease in respiratory quotient, indicating a decrease in the fraction of total energy derived from carbohydrate oxidation and an increase in the fraction derived from fatty acid oxidation. These metabolic variables were altered without affecting total metabolic rate. This dose of analogue also stimulated significantly greater food intake than injections of vehicle. These results support the continued use of 2,5-AM as a tool to probe the metabolic controls of food intake.

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