scholarly journals Seroprevalence, Risk Factors and Molecular Identification of Bovine Leukemia Virus in Egyptian Cattle

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 319
Author(s):  
Abdelfattah Selim ◽  
Eman A. Manaa ◽  
Abdullah D. Alanazi ◽  
Mohamed S. Alyousif
Keyword(s):  
Risk Factors ◽  
Dairy Cattle ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Significant Risk ◽  
Control Program ◽  
Effective Control ◽  
Economic Losses ◽  
Genetic Classification ◽  
Associated Risk Factors

Bovine leukemia virus (BLV) is distributed worldwide and affects dairy cattle causing severe economic losses. The BLV has been serologically reported in Egypt, but few studies have evaluated its associated risk factors and genetic classification. Therefore, this study assessed risk factors associated with BLV infection and identified the genetic diversity of the Egyptian strain. The study was conducted on 500 dairy cattle distributed in four Governorates located in Northern Egypt. Overall, the seroprevalence of BLV infection among Egyptian dairy cattle was 18.2%. The grazing cattle in the losing house system had higher odds for BLV seropositivity, and bad practice such as the use of a single needle or one plastic glove for more than one animal was considered a significant risk factor for BLV infection. Besides, the sequencing and phylogenetic analysis for one Egyptian BLV strain was performed, and the obtained results confirmed the clustering of Egyptian BLV strain into genotype-1. The assessment of associated risk factors for BLV infection and determination of its genetic classification are essential to implement an effective control program.

scholarly journals Sero-epidemiology and associated risk factors of brucellosis among sheep and goat population in the south western Nepal: a comparative study

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Tulsi Ram Gompo ◽  
Rubina Shah ◽  
Ishwari Tiwari ◽  
Yam Bahadur Gurung
Keyword(s):  
Risk Factors ◽  
Significant Risk ◽  
Small Ruminants ◽  
Control Program ◽  
Significant Risk Factor ◽  
Serum Samples ◽  
Cross Sectional ◽  
Sheep Population ◽  
Goat Population ◽  
Associated Risk Factors

Abstract Background Brucellosis is a zoonotic disease caused by Brucella spp. In Nepal, the presence of brucellosis in small ruminants, namely sheep and goats, has impacted farmers’ livelihood and the food safety of consumers. A cross-sectional study was conducted in Rupandehi district of Nepal during January to March 2020 to investigate the seroepidemiology and associated risk factors of brucellosis in the sheep and goat population. Altogether, 19 sheep and 60 goat farms in the district were visited. Owners were interviewed to get information on animals, including their management and movement patterns. Three hundred fifty-seven samples (80 sheep and 277 goat samples) were collected proportionately based on farm sizes. Each serum sample was tested with Rose Bengal Test and ELISA to estimate the seropositivity of brucellosis. Logistic regression was carried out to calculate corresponding odds ratios of each variable associated with detection of brucellosis. Results At the farm level, 31.6% (6/19; 95% CI: 12, 54%) of sheep farms and 3.3% (2/60, 95% CI: 0.9, 11.4%) of goat farms were seropositive to brucellosis. Out of 80 sheep serum samples, 12 (15%; 95% CI: 8.79–24.41%) and out of 277 goat serum samples, three (1.1%; 95% CI: 0.37–3.14%) were seropositive to brucellosis. Age greater than 1.5 years (OR = 5.56, 95% CI: 1.39, 29.38; p = 0.02) and herd size of greater than 100 (OR = 4.74, 95% CI: 1.23, 20.32, p = 0.03) were identified as significant risk factors for seropositivity of brucellosis in the sheep population. While in the goat population, none of the variables was identified as a significant risk factor. Conclusion The study provides evidence that the older sheep and the sheep from the large herds were at higher risk of brucellosis. A control program should be put in place immediately in the sheep population because they may transmit infections to other livestock as they were regularly moved for grazing and selling purposes. Also, strict biosecurity measures should be implemented among pastoralists to prevent brucellosis transmission in them. We suggest further one health-based study to reveal the transmission dynamics of brucellosis between animals and humans.

scholarly journals The genotype determination and molecular characterization of bovine leukemia virus in Turkey

2021 ◽  
Vol 91 (3) ◽  
pp. 237-247
Author(s):  
Feray Alkan ◽  
◽  
Ilke Karayel-Hacioglu ◽  
Selda Duran Yelken ◽  
Nuvit Coskun
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Control Program ◽  
Amino Acid Sequences ◽  
Economic Losses ◽  
Geographical Regions ◽  
Full Length Sequence

Bovine leukemia virus (BLV) infection is widespread worldwide and causes significant economic losses in the livestock industry. In this study, to identify the genotypes and to investigate the molecular characterization of BLV strains, we analyzed 25 BLV strains from cattle housed in six herds located in several Turkish geographical regions. Phylogenetic analysis, based on a partial or full-length sequence of the env gp51 gene of BLVs, showed that all Turkish BLVs belonged to genotype 1. The alignment of the deduced amino acid sequences demonstrated fourteen amino acid substitutions in different regions of the env gp51. This study updates our knowledge of BLV in Turkey. Further studies are needed to understand the molecular epidemiology and implement a comprehensive BLV control program in Turkey.

scholarly journals Sero-epidemiology and associated risk factors of brucellosis among sheep and goat population in the south western Nepal: A comparative study

2020 ◽  
Author(s):  
Tulsi Ram Gompo ◽  
Rubina Shah ◽  
Ishwari Tiwari ◽  
Yam Bahadur Gurung
Keyword(s):  
Risk Factors ◽  
Significant Risk ◽  
Small Ruminants ◽  
Control Program ◽  
Significant Risk Factor ◽  
Serum Samples ◽  
Cross Sectional ◽  
Sheep Population ◽  
Goat Population ◽  
Associated Risk Factors

Abstract BackgroundBrucellosis is a zoonotic disease of animals and humans caused by the Brucella spp. In Nepal, the presence of brucellosis in small ruminants, such as sheep and goats, has impacted farmers' livelihood and the food safety of the consumer. A cross-sectional study was conducted in the Rupandehi district of Nepal to investigate the seroepidemiology and associated risk factors of brucellosis in the sheep and goat population. Altogether, nineteen sheep and sixty goat farms located in the district were visited. The owners were interviewed to get information on animals, including their management and movement patterns. Three hundred fifty-seven samples (80 sheep and 277 goat samples) were collected proportionately based on the farm sizes. Each serum sample was tested with Rose Bengal Test and ELISA to estimate the seropositivity of brucellosis. Logistic regression was applied to calculate corresponding odds ratios of each variable associated with detection brucellosis. Results At the farm level, 31.6% (6/19; 95% CI: 12%, 54%) of sheep farms and 3.3% (2/60, 95% CI: 0.9%, 11.4 %) of the goat farms were seropositive to brucellosis. Out of 80 sheep serum samples, 12 (15%; 95% CI: 8.79%-24.41%) and out of 277 goat serum samples 3 (1.1%; 95% CI: 0.37%-3.14%) were seropositive to brucellosis. Age greater than 1.5 years (OR= 5.56, 95% CI: 1.39, 29.38, p = 0.02) and herd size of greater than a hundred (OR= 4.74, 95% CI: 1.23, 20.32, p = 0.03) were identified as significant risk factors for seropositivity of brucellosis in the sheep population. While in the goat population, none of the variables was identified as a significant risk factor. ConclusionThe study provides evidence that the older sheep and the sheep from the large herd sizes were at higher risk of detecting brucellosis. A control program should be applied immediately in the sheep population because they may transmit infections to other livestock as they were regularly moved for grazing and selling purposes. Also, strict biosecurity measures should be implemented among pastoralists to prevent brucellosis transmission in them. We suggest further one health-based study to reveal the transmission dynamics of brucellosis between animals and humans.

scholarly journals Mycobacterium aviumSubspeciesparatuberculosisand Bovine Leukemia Virus Seroprevalence and Associated Risk Factors in Commercial Dairy and Beef Cattle in Northern and Northeastern China

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Wu-Wen Sun ◽  
Wen-Fa Lv ◽  
Wei Cong ◽  
Qing-Feng Meng ◽  
Chun-Feng Wang ◽  
...  
Keyword(s):  
Risk Factors ◽  
Beef Cattle ◽  
Bovine Leukemia Virus ◽  
Large Scale ◽  
Leukemia Virus ◽  
Herd Size ◽  
Northeastern China ◽  
Herd Level ◽  
Source Sample ◽  
Associated Risk Factors

Mycobacterium aviumsubspeciesparatuberculosis(MAP) and bovine leukemia virus (BLV) are important pathogens, commonly responsible for economical loss to cattle farms all over the world, yet their epidemiology in commercial dairy and beef cattle in China is still unknown. Thus, from September 2013 to December 2014, a large-scale seroprevalence study was conducted to determine the seroprevalence and identify herd-level risk factors associated with MAP and BLV infection. The source sample was 3674 cattle from 113 herds in northern and northeastern China. Antibodies against MAP and BLV were detected using ELISA tests. At animal-level, the seroprevalence of antibodies against MAP and BLV was 11.79% (433/3674) and 18.29% (672/3674), respectively. At herd-level, the seroprevalence of antibodies against MAP and BLV was 20.35% and 21.24% (24/113), respectively. Herd size was identified to be associated with MAP infection while herd size and presence of cattle introduced from other farms were significantly associated with BLV infection. Further research is needed to confirm these findings and improve the knowledge of the epidemiology of these two pathogens in these regions and elsewhere in China.

scholarly journals Sero-epidemiology and associated risk factors of brucellosis among sheep and goat population in the south western Nepal: A comparative study

2021 ◽  
Author(s):  
Tulsi Ram Gompo ◽  
Rubina Shah ◽  
Ishwari Tiwari ◽  
Yam Bahadur Gurung
Keyword(s):  
Risk Factors ◽  
Significant Risk ◽  
Small Ruminants ◽  
Control Program ◽  
Significant Risk Factor ◽  
Serum Samples ◽  
Cross Sectional ◽  
Sheep Population ◽  
Goat Population ◽  
Associated Risk Factors

Abstract BackgroundBrucellosis is a zoonotic disease caused by Brucella spp. In Nepal, the presence of brucellosis in small ruminants, namely sheep and goats, has impacted farmers' livelihood and the food safety of consumers. A cross-sectional study was conducted in Rupandehi district of Nepal during January to March 2020 to investigate the seroepidemiology and associated risk factors of brucellosis in the sheep and goat population. Altogether, 19 sheep and 60 goat farms in the district were visited. Owners were interviewed to get information on animals, including their management and movement patterns. Three hundred fifty-seven samples (80 sheep and 277 goat samples) were collected proportionately based on farm sizes. Each serum sample was tested with Rose Bengal Test and ELISA to estimate the seropositivity of brucellosis. Logistic regression was carried out to calculate corresponding odds ratios of each variable associated with detection of brucellosis.Results At the farm level, 31.6% (6/19; 95% CI: 12%, 54%) of sheep farms and 3.3% (2/60, 95% CI: 0.9%, 11.4 %) of goat farms were seropositive to brucellosis. Out of 80 sheep serum samples, 12 (15%; 95% CI: 8.79%-24.41%) and out of 277 goat serum samples, three (1.1%; 95% CI: 0.37%-3.14%) were seropositive to brucellosis. Age greater than 1.5 years (OR= 5.56, 95% CI: 1.39, 29.38; p = 0.02) and herd size of greater than 100 (OR= 4.74, 95% CI: 1.23, 20.32, p = 0.03) were identified as significant risk factors for seropositivity of brucellosis in the sheep population. While in the goat population, none of the variables was identified as a significant risk factor.ConclusionThe study provides evidence that the older sheep and the sheep from the large herds were at higher risk of brucellosis. A control program should be put in place immediately in the sheep population because they may transmit infections to other livestock as they were regularly moved for grazing and selling purposes. Also, strict biosecurity measures should be implemented among pastoralists to prevent brucellosis transmission in them. We suggest further one health-based study to reveal the transmission dynamics of brucellosis between animals and humans.

scholarly journals Bayesian Estimation of the True Seroprevalence and Risk Factor Analysis of Bovine Leukemia Virus Infection in Pakistan

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1404
Author(s):  
Ali Sakhawat ◽  
Marzena Rola-Łuszczak ◽  
Zbigniew Osiński ◽  
Nazia Bibi ◽  
Jacek Kuźmak
Keyword(s):  
Risk Factor ◽  
Dairy Cattle ◽  
Bovine Leukemia Virus ◽  
Water Buffalo ◽  
Leukemia Virus ◽  
Credible Interval ◽  
Indigenous Cattle ◽  
Herd Level ◽  
Exotic Breed ◽  
Posterior Estimate

The objective of this study was to determine the true seroprevalence of bovine leukemia virus (BLV) infection in dairy cattle from Pakistan at the animal and herd-level. We tested 1380 dairy cattle from 451 herds and 92 water buffalo. The sera were tested by ELISA and the results were analyzed using Bayesian inference. The median posterior estimate of the herd level true BLV prevalence was 1.4%, with a 95% credible interval (CI) 0.7–3.1, whereas the median posterior estimate of the within-farm true seroprevalence was 3.8% with a 95% CI 2.8–4.8. All 92 sera collected from water buffalo were negative. Several risk factors potentially associated with seropositivity to BLV infections in Pakistan were analyzed using logistic regression model based on calculation of an odds ratio (OR). The study showed an association between seropositivity and medium herd (≥50) size (OR = 23.57, 95% CI: 3.01–103.48). Common housing of indigenous cattle with exotic-breed cattle (OR = 0.67, 95% CI: 06–2.35) or housing indigenous or their crossbred cattle with exotic-breed cattle (OR = 0.95, 95% CI: 0.14–3.01) had no effect on the BLV seroprevalence. Similarly, common housing of cattle and water buffalo was not risk factor for increased BLV seropositivity (OR = 27.10, 95% CI: 0.63–119.34).

Prevalence and risk factors of root resorption of adjacent teeth in maxillary canine impaction, among untreated children and adolescents

2018 ◽  
Vol 41 (5) ◽  
pp. 447-453 ◽  
Author(s):  
Frédéric Rafflenbeul ◽  
Catherine-Isabelle Gros ◽  
François Lefebvre ◽  
Sophie Bahi-Gross ◽  
Raphaëlle Maizeray ◽  
...  
Keyword(s):  
Risk Factors ◽  
Children And Adolescents ◽  
Root Resorption ◽  
Significant Risk ◽  
Significant Risk Factor ◽  
Data Sets ◽  
Maxillary Canine ◽  
3D Data ◽  
Potential Risk Factors ◽  
Associated Risk Factors

Summary Objectives The aim of this retrospective study was to assess in maxillary canine impaction cases both the prevalence of root resorption of adjacent teeth among untreated children and adolescents, and its associated risk factors. Subjects and methods Sixty subjects (mean age 12.2 years; SD 1.9; range 8–17 years) with 83 displaced maxillary canines and without any past or ongoing orthodontic treatment were included in this study. The presence of root resorption was evaluated on images from a single cone beam computed tomography (CBCT) unit. Potential risk factors were measured on the CBCT images and on panoramic reconstructions of the 3D data sets. The sample was characterized by descriptive statistics and multiple logistic regressions were performed to predict root resorption. Results Root resorption of at least one adjacent tooth was detected in 67.5 per cent of the affected quadrants. It was found that 55.7 per cent of the lateral incisors, 8.4 per cent of the central incisors, and 19.5 per cent of first premolars were resorbed. Of the detected resorptions, 71.7 per cent were considered slight, 14.9 per cent moderate, and 13.4 per cent severe. Contact between the displaced canine(s) and the adjacent teeth roots was the only identified statistically significant risk factor, all teeth being considered (odds ratio [OR] = 18.7, 95% confidence interval: 2.26–756, P < 0.01). An enlarged canine dental follicle, a peg upper lateral, or an upper lateral agenesis were not significantly associated with root resorption of adjacent teeth, nor were age nor gender. Conclusions Root resorption of adjacent teeth was detected in more than two-thirds of a sample of sixty untreated children and adolescents.

scholarly journals Sero-Prevalence of Contagious Caprine Pleuropneumonia in Goats in Afgoye District Lower Shabelle Region, Somalia

2020 ◽  
Vol 5 (2) ◽  
pp. 1-4
Author(s):  
Omar Mohamed Abdi
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Latex Agglutination ◽  
Economic Losses ◽  
High Morbidity ◽  
Cross Sectional ◽  
Mycoplasma Capricolum ◽  
Contagious Caprine Pleuropneumonia ◽  
Epidemiological Risk ◽  
Associated Risk Factors

Background: Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. Capripneumoniae (Mccp), is one of the most important goat diseases causing high morbidity and mortality, these results in heavy economic losses to countries like Somalia, which is a country affected the civil war of the 1990s. Objective: Generally, the disease presence has been reported in the country. However, there is no quantitative data on the prevalence and distribution of the disease has been scientifically reported in the country. Methods: A cross-sectional study was conducted from September 2019 to June 2020 in three villages of Afgoye district of Somalia to assess the seroprevalence and identify the associated risk factors for the occurrence of the CCPP. The risk factors considered included sex, age, and locations. Results: A total of 100 blood samples were collected and evaluated by Capri-Latex Agglutination Test (Capri-LAT). A total of 49/100 (49%, CI 95%: (38.86%-59.2%) goats were seropositive for anti-CCPP antibody. The epidemiological risk factors considered, sex, age and locations, were not significantly associated with the CCPP sero prevalence (p>0.05). However, the sero positivity was slightly higher in female (51%, 95% CI: 39.8-62.6), >3 years old goats (86.3%, 95% CI: 48.9-87.4) and Markazka village (56%, 95% CI: 41.3-70.0) compared to male (40%, 95% CI: 19.1-63.9), 1-3 years old (57.5%, 95% CI: 40.9- 73.0), and Shareeco (43.8%, 95% CI: 19.8-70.1) and Siinka (41.2%, 95% CI: 24.7-59.3) villages, respectively. Conclusion: The present study indicates the prevalence of CCPP in the studies areas and this requires practicable measures to be put in place towards the control of the disease.

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