scholarly journals The Occurrence of Mycobacterium avium Subspecies paratuberculosis Positive Milk Antibody ELISA Results in Dairy Cattle under Varying Time Periods after Skin Testing for Bovine Tuberculosis

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1224
Author(s):  
Nicole Bridges ◽  
Steven van Winden
Keyword(s):  
Mycobacterium Avium ◽  
Bovine Tuberculosis ◽  
Management Practices ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Skin Testing ◽  
Apparent Prevalence ◽  
Elisa Testing ◽  
History Of ◽  
Antibody Elisa ◽  
Milk Elisa

Enzyme-linked immunosorbent assays (ELISA) are used to screen cows for Mycobacterium avium subspecies paratuberculosis (MAP) infections, informing Johne’s disease (JD) management practices in dairy herds. The causative agent of bovine tuberculosis (bTB), Mycobacterium bovis, and MAP share multiple antigens. Moreover, Mycobacterium avium subspecies avium is used in the single intradermal cervical comparative tests (SICCT) that are routinely used in early detection of cows infected with bTB. Although these are different types of immune responses, potentially the SICCT may interfere with the levels of MAP antibodies. This study aimed to clarify the relationship between the SICCT-MAP milk ELISA testing interval and apparent prevalence of JD risk statuses. Data from 51 herds were used, totalling 46,738 cow observations. The Poisson models showed that MAP milk ELISA testing at 14 day intervals post-SICCT statistically significantly increased the odds of detecting JD-positive cows compared to JD testing 85+ days post-SICCT. The odds ratio (OR) started at 2.5 in the first 14 day interval post-SICCT, increasing each two-week period to an OR of 4.0 at 57–70 days, to subsequently drop. Additionally, a herd history of bTB increased the odds of detecting JD-positive cows (OR = 1.2); this was relatively limited compared to the magnitude of the post-SICCT effect.

scholarly journals Demonstration of Circulating Antibodies of Mycobacterium avium Subspecies paratuberculosis in Cattle of Rupandehi District, Nepal

2019 ◽  
Vol 36 ◽  
pp. 23-29
Author(s):  
M. L. Sharma ◽  
M. Prajapati ◽  
Y. Panth
Keyword(s):  
Body Condition ◽  
Mycobacterium Avium ◽  
Body Condition Score ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Close Association ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chronic Diarrhoea ◽  
Test Kit ◽  
Condition Score ◽  
History Of

Paratuberculosis, caused by Mycobacterium avium subspp. paratuberculosis, is a chronic intestinal infection of global importance in mainly domestic and wild ruminants. The main objective of the study was to find out the seroprevalence of Paratuberculosis in cattle of Rupandehi district. The research was conducted from October 2016 to December 2016. A total of 184 blood samples were collected from Jugular vein of cattle and tested by Enzyme Linked Immunosorbent Assay (ELISA). The Paratuberculosis Indirect Screening Test Kit was developed by ID. Vet, France. Cattle with history of chronic diarrhoea and emaciation were taken as study population along with other cattle in close association with them. Overall seroprevalence in Rupandehi district was found to be 4.89%. No significant relation of paratuberculosis was found with age, breed, parity, body condition score and location. Higher prevalence was found in cattle of older age and low body condition score. The result of this study reports the presence of bovine paratuberculosis in cattle of Rupandehi district.

scholarly journals Seroprevalence of Mycobacterium avium subsp. paratuberculosis in Dairy Cattle in Khartoum State, Sudan

2020 ◽  
Vol 7 (4) ◽  
pp. 209
Author(s):  
Wisal A. Elmagzoub ◽  
Nabawia M. Adam ◽  
Sanaa M. Idris ◽  
Mohamed E. Mukhtar ◽  
Sanaa A. Abdelaziz ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Animal Health ◽  
Herd Size ◽  
Low Fertility ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
African Countries ◽  
Apparent Prevalence ◽  
World Organization

Paratuberculosis, caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a chronic wasting disease mainly of domestic and wild ruminants. It occurs worldwide, causing significant economic losses through decreased productivity, low fertility, increased cull rates and mortality. It is listed by the OIE (World Organization for Animal Health) as a disease of concern to trade in animals. Prevalence of this disease can be studied by detecting anti-MAP antibodies by Enzyme linked immunosorbent Assay (ELISA). The aim of this study was to investigate the current prevalence of MAP infection in cattle in Khartoum State. The overall apparent prevalence of MAP infection was found to be 6.3% and 18.9% at animal and herd levels, respectively. All seropositive animals were cross-bred females of good body condition; most of them (>90%) were >3 years old and >50% were from medium-sized herds in Omdurman. No significant association (p > 0.05) was found between seropositivity and animal herd size. The prevalence of MAP infection in Khartoum State is still low to medium compared to other parts of the world, but it is comparable to those reported from other African countries. Further studies with the view of designing nationwide surveys in domestic ruminants and camels in other states of the country are needed for establishing control programmes.

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