scholarly journals Diets for Dairy Cows with Different Proportions of Crude Protein Originating from Red Clover Silage versus Soybean Meal: Ruminal Degradation and Intestinal Digestibility of Amino Acids

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2177
Author(s):  
Edwin Westreicher-Kristen ◽  
Ralf Blank ◽  
Monika Paschke-Beese ◽  
Wiebke Kühl ◽  
Siegfried Wolffram ◽  
...  

The purpose was to assess the effect of exchanging crude protein (CP) of soybean meal (SBM) with red clover silage (RCS) in total mixed rations (TMR) on ruminal degradation and intestinal digestibility (ID) of essential amino acids (EAA). Four TMR and their individual feed components were studied. The TMR were composed of forage and concentrates (75:25), with proportions of RCS in TMR of 0.15, 0.30, 0.45, and 0.60 on a dry matter basis, resulting in diet groups RCS15, RCS30, RCS45, and RCS60, respectively. The ruminal degradation of EAA was determined using the nylon bag technique. For this, samples of TMR and their individual feed components were ruminally incubated for 16 h. The feed residues of TMR obtained after 16 h of incubation were used for the determination of ID of EAA using the mobile-bag technique. Increasing RCS and reducing SBM proportions linearly increased (p < 0.01) the in situ ruminal degradation of individual EAA from 75.5% to 83.5%. The degradation of EAA followed the trend of CP degradation among TMR, except for Cys that was lower (p < 0.05) than that of CP in RCS60 (79.7% vs. 86.3%). The degradation of EAA in individual feed ingredients not always corresponded to the degradation of CP and was feed dependent. Increasing the proportions of RCS in the TMR linearly reduced (p < 0.001) the ID of EAA (except for Ile) from 78.2% to 67.3%. However, the ID of EAA did not always reflect the ID of CP, and in general, the differences between the ID of CP and EAA increased as RCS increased in the TMR. The ID values of most of the EAA were similar (p > 0.05) to ID of CP in RCS15 and RCS30, while they mostly differed (p < 0.05) in RCS45 and RCS60, and being higher for EAA than CP (except for Cys that was lower than CP, p < 0.05). Similar trends were observed for intestinal absorbable AA, resulting in higher values (p < 0.05) of intestinal absorbable for all EAA than of CP in diet RCS60. In conclusion, increasing levels of RCS in TMR reduced the extent of EAA flow into the small intestine, the ID of EAA, and consequently the intestinal absorbable EAA. Therefore, accurate determination of metabolizable AA must be considered for optimal diet formulation when including high proportions of RCS in diets of high-producing dairy cows.

2012 ◽  
Vol 92 (2) ◽  
pp. 211-217 ◽  
Author(s):  
L. Doepel ◽  
J. E. Montgomery ◽  
K. A. Beauchemin ◽  
J. R. King ◽  
S. N. Acharya

Doepel, L., Montgomery, J. E., Beauchemin, K. A., King, J. R. and Acharya, S. N. 2012. Ruminal degradability and whole-tract digestibility of protein and fibre fractions in fenugreek haylage. Can. J. Anim. Sci. 92: 211–217. The purpose of this study was to evaluate the digestibility of fenugreek forage compared with alfalfa in dairy cows. Alfalfa and two genotypes of fenugreek, Quatro and F70, were harvested and prepared as plastic-wrapped high-moisture bales. Fourteen weeks after baling, forage samples were obtained for determination of ruminal degradation and whole-tract digestibility. To determine ruminal degradation, forage samples were placed in polyester bags and incubated in the rumen for various time points (up to 168 h) in lactating Holstein cows. Digestibility in the rumen, intestine and the whole digestive tract was estimated by incubating samples in the rumen, followed by measurements of intestinal digestibility using the mobile bag technique in two non-lactating Holstein cows. Effective ruminal degradability of DM was lower for F70 than for alfalfa, while for ADF and NDF it was equivalent in all forages. Whole-tract disappearance of DM, CP, ADF and NDF was lower for F70 than Quatro. In general, Quatro and alfalfa were similar in terms of ruminal degradation and digestion in the rumen, intestine and whole-tract, while F70 tended to be of lower quality.


2009 ◽  
Vol 88 (6) ◽  
pp. 1216-1226 ◽  
Author(s):  
A.M. Waguespack ◽  
S. Powell ◽  
T.D. Bidner ◽  
R.L. Payne ◽  
L.L. Southern

2018 ◽  
Vol 245 ◽  
pp. 126-135 ◽  
Author(s):  
Edwin Westreicher-Kristen ◽  
Ralf Blank ◽  
Cornelia C. Metges ◽  
Andreas Susenbeth

2012 ◽  
Vol 41 (3) ◽  
pp. 717-725 ◽  
Author(s):  
Lidia Ferreira Miranda ◽  
Norberto Mario Rodriguez ◽  
Elzânia Sales Pereira ◽  
Augusto César de Queiroz ◽  
Roberto Daniel Sainz ◽  
...  

2017 ◽  
Vol 28 (2) ◽  
pp. 341
Author(s):  
Monica Duque Quintero ◽  
Ricardo Rosero Noguera ◽  
Marta Olivera Ángel

The aim of this study was to determine the digestion of dry matter (MS), crude protein (PC) and amino acids (AA) in dairy cows. Two cannulated cows were used for the determination of passage rate (Kp), in situ ruminal degradability (DR) and intestinal digestibility (DI) by abomasal catheter. The data to calculate Kp was analyzed with NLIN procedure by SAS, and descriptive statistics for DR and DI of MS, PC and AA from Kikuyu grass (Pennisetum clandestinum), a commercial supplement and two sources of rumen-protected AA. The study showed a Kp and a ruminal retention time of 0.036 h-1 and 27.4 h. The highest values from DR of MS and CP were from Kikuyu grass (69.0 and 61.8%) and concentrate (84.7 and 77.2%), followed by MetP (60.2 and 66.7%) and LysP (6.72 and 11.4%). The highest percentages of rumen indegradable amino acids (AADR) were from Kikuyu and concentrate, varying between 58.7 and 68% in forage, and 76.1 and 82.9% in the concentrate. The DR was 11.5 and 65.8% in LysP and MetP, respectively. The DI of AA (%AADR) varied between 42.3 and 77.4% for Kikuyu and 42.2 and 59.3% for concentrate. The values for the protected amino acids were 42.1 for LysP and 58.6 for MetP. 


2006 ◽  
Vol 82 (1) ◽  
pp. 75-81 ◽  
Author(s):  
J. González ◽  
J. Faría-Mármol ◽  
C.A. Rodríguez ◽  
M. Ouarti ◽  
M.R. Alvir ◽  
...  

AbstractThe effective ruminal degradability (ED) of dry matter (DM), crude protein (CP) and amino acids, and the effective intestinal digestibility (IED) of DM and CP of a sample of whole cottonseed was measured using in situ and rumen outflow rate techniques in three wethers cannulated in the rumen and duodenum. The microbial contamination of rumen incubated residues was corrected by a continuous rumen infusion of15NH3as microbial marker and rumen solid associated bacteria as reference sample. Microbial contamination resulted in an overestimation of the undegradable fraction of DM (0·291v. 0·275;P<0·05) and CP (0·071v. 0·037;P<0·01) and a small underestimation of ED of DM (0·500v. 0·512;P=0·09) and CP (0·755v. 0·779;P=0·052). A proportion of 0·1 of the ruminal undegraded CP was of microbial origin and for essential amino acids this proportion varied from 0·042 to 0·150. Differences in ED between amino acids modified the amino acid profile, with an important reduction (0·2;P<0·01) in the proportion of lysine. Apparent intestinal digestibility of the insoluble fraction of this food, measured with the mobile nylon bag technique, showed large reductions (P<0·001) with the increase of the ruminal incubation time between 0 and 72 h: from 0·392 to 0·026 for DM and from 0·851 to 0·099 for CP. These evolutions fitted an exponential function with a previous lag. The IED was estimated either by integration of these equations and those describing the ruminal degradation and rumen outflow or by incubation through the intestines of a sample pooled to be representative of rumen flow of the undegraded food. The two methods gave similar values for both DM (0·222v. 0·203) and CP (0·659v. 0·658).


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sonia Yun Liu ◽  
Shemil P. Macelline ◽  
Peter V. Chrystal ◽  
Peter H. Selle

AbstractThe prime purpose of this review is to explore the pathways whereby progress towards reduced-crude protein (CP) diets and sustainable chicken-meat production may be best achieved. Reduced-CP broiler diets have the potential to attenuate environmental pollution from nitrogen and ammonia emissions; moreover, they have the capacity to diminish the global chicken-meat industry’s dependence on soybean meal to tangible extents. The variable impacts of reduced-CP broiler diets on apparent amino acid digestibility coefficients are addressed. The more accurate identification of amino acid requirements for broiler chickens offered reduced-CP diets is essential as this would diminish amino acid imbalances and the deamination of surplus amino acids. Deamination of amino acids increases the synthesis and excretion of uric acid for which there is a requirement for glycine, this emphasises the value of so-called “non-essential” amino acids. Starch digestive dynamics and their possible impact of glucose on pancreatic secretions of insulin are discussed, although the functions of insulin in avian species require clarification. Maize is probably a superior feed grain to wheat as the basis of reduced-CP diets; if so, the identification of the underlying reasons for this difference should be instructive. Moderating increases in starch concentrations and condensing dietary starch:protein ratios in reduced-CP diets may prove to be advantageous as expanding ratios appear to be aligned to inferior broiler performance. Threonine is specifically examined because elevated free threonine plasma concentrations in birds offered reduced-CP diets may be indicative of compromised performance. If progress in these directions can be realised, then the prospects of reduced-CP diets contributing to sustainable chicken-meat production are promising.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 175-175
Author(s):  
Élisabeth Chassé ◽  
Frédéric Guay ◽  
Marie-Pierre Létourneau-Montminy

Abstract This study was conducted to determine the effect of pelleting on the digestibility of corn-soybean meal-based diet in growing pigs. Two trials with 6 pigs cannulated at the distal ileum were conducted. In each trial, pigs were assigned to each treatment following a crossover design. In each experiment, the same diet, composed of corn and soybean meal with 10% wheat from two different feed mills, was served in pellet or mash form. Pelleting allowed an increase in digestibility in one of the trials. Apparent ileal digestibility (AID) of dry matter (DM), crude protein (CP) and digestible energy (DE) were improved with pelleting by 8, 12 and 9% (P&lt; 0.01). The AID of amino acids (AA) was also improved (P&lt; 0.05). Apparent total tract digestibility (ATTD) was increased by pelleting in DM, CP and DE by 5, 7 and 6% respectively (P&lt; 0.01). The digestibility of the mash diet in experiment 1 was lower than in the pelleted diet in the experiment 1 and both diets in experiment 2 as shown by the interaction Pelleting X Trial which was significant for the AID and ATTD of DM, CP and DE (P&lt; 0.01). Therefore, in experiment 1, pelleting allows to improve the digestibility of diet to the same level as in experiment 2. The AID of CP was higher by 37% in the mash diet from the second experiment compared to the one in the first experiment. Even though the same ingredients were chosen in the two experiments, this shows the variability in digestibility existing between different feed mills and ingredient sources. This difference was not observed in pelleted diets. The results obtained in these two trials show that pelleting can reduce the variability of digestibility and then give a good digestibility of diets even if the ingredients are of different quality or sources.


Sign in / Sign up

Export Citation Format

Share Document