scholarly journals Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2949
Author(s):  
Theerawat Tharasanit ◽  
Paweena Thuwanut

Oocyte cryopreservation plays important roles in basic research and the application of models for genetic preservation and in clinical situations. This technology provides long-term storage of gametes for genetic banking and subsequent use with other assisted reproductive technologies. Until recently, oocytes have remained the most difficult cell type to freeze, as the oocytes per se are large with limited surface area to cytoplasm ratio. They are also highly sensitive to damage during cryopreservation, and therefore the success rate of oocyte cryopreservation is generally poor when compared to noncryopreserved oocytes. Although advancement in oocyte cryopreservation has progressed rapidly for decades, the improvement of cryosurvival and clinical outcomes is still required. This review focuses on the principles, techniques, outcomes and prospects of oocyte cryopreservation in domestic animals and humans.

Biomolecules ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 181 ◽  
Author(s):  
Vanesa Robles ◽  
David G. Valcarce ◽  
Marta F. Riesco

The cryopreservation of gametes and embryos is a technique widely used in reproductive biology. This technology helps in the reproductive management of domesticated animals, and it is an important tool for gene banking and for human-assisted reproductive technologies. Antifreeze proteins are naturally present in several organisms exposed to subzero temperatures. The ability for these proteins to inhibit ice recrystallization together with their ability to interact with biological membranes makes them interesting molecules to be used in cryopreservation protocols. This mini-review provides a general overview about the use of antifreeze proteins to improve the short and long term storage of gametes and embryos.


HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1088B-1088
Author(s):  
Christian Krarup ◽  
Oscar Núñez

Chilling injury of cantaloupe melons (Cucumismelo, Cantalupensis group), although variable among cultivars, precludes the use of temperatures close to 0 °C for long term storage or transport. Diseases, either associated with chilling or independent from it, are usually the main factor terminating postharvest life of these melons. `Colima', a Western Shipper cultivar highly sensitive to chilling, was used to evaluate chilling and disease response to the following treatments: immersion in water at 60 °C for 1 minute (with or without 150 ppm chlorine), individual packaging (PVC, whole or perforated), exposure to air at 38 °C for 12 hours (with or without individual packaging), and control. After storage for 18 days at 0 °C and a simulated retail period of 3 days at 20 °C, there were significant differences among treatments: chilling was alleviated in heat-treated and especially in plastic-wrapped fruit, and the presence of diseases caused by pathogens such as Alternaria, Cladosporium, Fusarium, and Rhizopus was markedly reduced by heat treatments. Overall, visual quality was high and superior in fruit immersed in water at 60 °C with 150 ppm chlorine due to almost complete supression of diseases. Project financed by FONDECYT 1020882.


2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.


Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  

2020 ◽  
Vol 59 (SL) ◽  
pp. SLLC01 ◽  
Author(s):  
Tomoki Murota ◽  
Toshiki Mimura ◽  
Ploybussara Gomasang ◽  
Shinji Yokogawa ◽  
Kazuyoshi Ueno

Author(s):  
O. Semenenko ◽  
O. Vodchyts ◽  
V. Koverga ◽  
R. Lukash ◽  
O. Lutsenko

The introduction and active use of information transmission and storage systems in the Ministry of Defense (MoD) of Ukraine form the need to develop ways of guaranteed removal of data from media after their use or long-term storage. Such a task is an essential component of the functioning of any information security system. The article analyzes the problems of guaranteed destruction of information on magnetic media. An overview of approaches to the guaranteed destruction of information on magnetic media of different types is presented, and partial estimates of the effectiveness of their application are given by some generally accepted indicators of performance evaluation. The article also describes the classification of methods of destruction of information depending on the influence on its medium. The results of the analysis revealed the main problems of application of software methods and methods of demagnetization of the information carrier. The issue of guaranteed destruction of information from modern SSD devices, which are actively used in the formation of new systems of information accumulation and processing, became particularly relevant in the article. In today's conditions of development of the Armed Forces of Ukraine, methods of mechanical and thermal destruction are more commonly used today. In the medium term, the vector of the use of information elimination methods will change towards the methods of physical impact by the pulsed magnetic field and the software methods that allow to store the information storage device, but this today requires specialists to develop new ways of protecting information in order to avoid its leakage.


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