scholarly journals Virulence Determinants and Antimicrobial Profiles of Pasteurella multocida Isolated from Cattle and Humans in Egypt

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 480
Author(s):  
Mohamed Sabry Abd Elraheam Elsayed ◽  
Samah Mahmoud Eldsouky ◽  
Tamer Roshdy ◽  
Lamia Said ◽  
Nahed Thabet ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Efflux Pump ◽  
Virulence Gene ◽  
Molecular Classification ◽  
Multiple Drug ◽  
Virulence Determinants ◽  
Type D ◽  
Multiple Drug Resistant ◽  
Class 1 ◽  
Methylase Gene

Pasteurella multocida is a Gram-negative bacterium that causes drastic infections in cattle and humans. In this study, 55 isolates were recovered from 115 nasal swabs from apparently healthy and diseased cattle and humans in Minufiya and Qalyubia, Egypt. These isolates were confirmed by kmt1 existence, and molecular classification of the capsular types showed that types B, D, and E represented 23/55 (41.8%), 21/55 (38.1%), and 11/55 (20.0%), respectively. The isolates were screened for five virulence genes with hgbA, hgbB, and ptfA detected in 28/55 (50.9%), 30/55 (54.5%), and 25/55 (45.5%), respectively. We detected 17 capsular and virulence gene combinations with a discriminatory power (DI) of 0.9286; the most prevalent profiles were dcbF type D and dcbF type D, hgbA, hgbB, and ptfA, which represented 8/55 (14.5%) each. These strains exhibited high ranges of multiple antimicrobial resistance indices; the lowest resistances were against chloramphenicol, ciprofloxacin, amoxicillin/clavulanic acid, and levofloxacin. The macrolide–lincosamide–streptogramin B methylase gene erm(Q), with erm(42) encoding MLSB monomethyltransferase, mph(E) encoding a macrolide efflux pump, and msr(E) encoding macrolide-inactivating phosphotransferase were present. The class 1 and 2 integrons and extended-spectrum β-lactamase genes intl1, intl2, blaCTX-M, blaCTX-M-1, and blaTEM were detected. It is obvious to state that co-occurrence of resistance genes resulted in multiple drug-resistant phenotypes. The identified isolates were virulent, genetically diverse, and resistant to antimicrobials, highlighting the potential risk to livestock and humans.

scholarly journals Isolation and Characterization of VceC Gain-of-Function Mutants That Can Function with the AcrAB Multiple-Drug-Resistant Efflux Pump of Escherichia coli

2006 ◽  
Vol 188 (11) ◽  
pp. 3757-3762 ◽  
Author(s):  
Govindsamy Vediyappan ◽  
Tatyana Borisova ◽  
Joe A. Fralick
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Efflux Pump ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Membrane Component ◽  
Gain Of Function ◽  
Isolation And Characterization ◽  
Multiple Drug Resistant ◽  
Major Facilitator

ABSTRACT VceC is the outer membrane component of the major facilitator (MF) VceAB-VceC multiple-drug-resistant (MDR) efflux pump of Vibrio cholerae. TolC is the outer membrane component of the resistance-nodulation-division AcrAB-TolC efflux pump of Escherichia coli. Although these proteins share little amino acid sequence identity, their crystal structures can be readily superimposed upon one another. In this study, we have asked if TolC and VceC are interchangeable for the functioning of the AcrAB and VceAB pumps. We have found that TolC can replace VceC to form a functional VceAB-TolC MDR pump, but VceC cannot replace TolC to form a functional AcrAB-VceC pump. However, we have been able to isolate gain-of-function (gof) VceC mutants which can functionally interface with AcrAB. These mutations map to four different amino acids located at the periplasmic tip of VceC. Chemical cross-linkage experiments indicate that both wild-type and gof mutant VceC can physically interact with the AcrAB complex, suggesting that these gof mutations are not affecting the recruitment of VceC to the AcrAB complex but rather its ability to functionally interface with the AcrAB pump.

scholarly journals Whole genome analysis of ExPEC ST73 from a single hospital over a 2-year period identified different circulating clonal groups

2018 ◽  
Author(s):  
DR Bogema ◽  
J McKinnon ◽  
M Liu ◽  
N Hitchick ◽  
N Miller ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Mobile Genetic Elements ◽  
Bootstrap Support ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Genetic Elements ◽  
Multiple Drug Resistant ◽  
Class 1 ◽  
Tract Infections ◽  
Sydney Australia

AbstractST73 has emerged as one of the most frequently isolated extraintestinal pathogenic E. coli (ExPEC). To examine the localised diversity of ST73 clonal groups including their mobile genetic elements profile, we sequenced the genomes of 16 multiple drug-resistant ST73 isolates from patients with urinary tract infection from a single hospital in Sydney, Australia between 2009 and 2011. Genome sequences were used to generate a SNP-based phylogenetic tree to determine the relationship of these isolates in a global context with ST73 sequences (n=210) from public databases. There was no evidence of a dominant outbreak strain of ST73 in patients from this hospital, rather we identified at least eight separate groups, several of which reoccur, over a two-year period. The inferred phylogeny of all ST73 strains (n=226) including the ST73 Clone D i2 reference genome shows high bootstrap support and clusters into four major groups which correlate with serotype. The Sydney ST73 strains carry a wide variety of virulence-associated genes but the presence of iss, pic and several iron acquisition operons was notable.ImpactST73 is a major clonal lineage of ExPEC that causes urinary tract infections often with uroseptic sequelae but has not garnered substantial scientific interest as the globally disseminated ST131. Isolation of multiple antimicrobial resistant variants of ExPEC ST73 have increased in frequency, but little is known about the carriage of class 1 integrons in this sequence type and the plasmids that are likely to mobilise them. This pilot study examines the ST73 isolates within a single hospital in Sydney Australia and provides the first large-scale core-genome phylogenetic analysis of ST73 utilizing public sequence read datasets. We used this analysis to identify at least 8 sub-groups of ST73 within this single hospital. Mobile genetic elements associated with antibiotic resistance were less diverse and only three class 1 integron structures were identified, all sharing the same basic structure suggesting that the acquisition of drug resistance is a recent event. Genomic epidemiological studies are needed to further characterise established and emerging clonal populations of multiple drug resistant ExPEC to identify sources and aid outbreak investigations.

scholarly journals Class 1 Integron-Borne Multiple-Antibiotic Resistance Carried by IncFI and IncL/M Plasmids in Salmonella enterica Serotype Typhimurium

1998 ◽  
Vol 42 (12) ◽  
pp. 3053-3058 ◽  
Author(s):  
Fabio Tosini ◽  
Paolo Visca ◽  
Ida Luzzi ◽  
Anna Maria Dionisi ◽  
Cristina Pezzella ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Multiple Drug Resistant ◽  
Class 1 ◽  
Mobile Gene

ABSTRACT The presence and genetic content of integrons were investigated for 37 epidemiologically unrelated multiple-drug-resistant strains ofSalmonella enterica serotype Typhimurium from humans. All isolates were resistant to ampicillin, chloramphenicol, kanamycin, streptomycin, sulfonamides, and trimethoprim, as well as to tetracycline and/or nalidixic acid; 20% of them were also resistant to gentamicin and amikacin. Three different class 1 integrons (In-t1, In-t2, and In-t3) were identified by Southern blot hybridization, PCR, and DNA sequencing, and these integrons were found to carry theaadB, catB3, oxa1,aadA1a, aacA4, and aacC1 gene cassettes. Integrons In-t1 (aadB and catB3) and In-t2 (oxa1 and aadA1a) were both located on a conjugative IncFI plasmid of 140 kb. In-t3 (aacA4,aacC1, and aadAIa) was located on an IncL/M plasmid of 100 kb which was present, in association with the IncFI plasmid, in gentamicin- and amikacin-resistant isolates. Despite the extensive similarity at the level of the antibiotic resistance phenotype, integrons were not found on the prototypic IncFI plasmids carried by epidemic Salmonella strains isolated during the late 1970s. The recent appearance and the coexistence of multiple integrons on two conjugative plasmids in the sameSalmonella isolate are examples of how mobile gene cassettes may contribute to the acquisition and dissemination of antibiotic resistance.

Synthesis and Anti-mycobacterium Study on Halo-substituted 2-aryl oxyacetohydrazones

2020 ◽  
Vol 16 (5) ◽  
pp. 618-628 ◽  
Author(s):  
Vijay J. Desale ◽  
Suraj N. Mali ◽  
Hemchandra K. Chaudhari ◽  
Maya C. Mali ◽  
Bapu R. Thorat ◽  
...  
Keyword(s):  
Uv Spectroscopy ◽  
Health Concern ◽  
Multiple Drug ◽  
Resistant Tuberculosis ◽  
Multiple Drug Resistant ◽  
H37rv Strain ◽  
Mdr Tb ◽  
Drug Regimens ◽  
Ft Ir ◽  
Mycobacteria Tuberculosis

Background: The treatment of multiple-drug-resistant tuberculosis (MDR-TB) with currently available marketed drugs remains a global health concern. The cases of resistant tuberculosis patients are increasing day by day. Objective: The objective of this study is to highlight the need of developing shorter, simpler and tolerable drug regimens. Methods: In the present study, we synthesized various halo-substituted 2-aryloxyacetohydrazones via a series of reactions from halo-substituted phenols. All the compounds were characterized by using various spectroscopic methods, such as NMR, FT-IR, UV spectroscopy, etc. Results: All the synthesized hydrazones showed theoretically good interactions with enzyme enoyl reductase (pdb id: 4tzk). All the synthesized compounds (5a-5o) showed moderate to good activity (3.125-100 μg/mL) against Mycobacteria tuberculosis, H37RV strain. Conclusion: Our results would pave a new way for the development of more effective Anti-TB agents in the future.

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