scholarly journals Oleanolic Acid Nanofibers Attenuated Particulate Matter-Induced Oxidative Stress in Keratinocytes

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1411
Author(s):  
Hsuan Fu ◽  
Feng-Lin Yen ◽  
Pao-Hsien Huang ◽  
Chun-Yin Yang ◽  
Chia-Hung Yen
Keyword(s):  
Oxidative Stress ◽  
Particulate Matter ◽  
Oleanolic Acid ◽  
Water Solubility ◽  
Airborne Particulate Matter ◽  
Skin Penetration ◽  
Skin Aging ◽  
Particle Size Reduction ◽  
Amino Terminal ◽  
Penetration Ability

Airborne particulate matter (PM) is one of the indicators of air pollution, and it is also the main factor causing oxidative stress in the skin. Oleanolic acid (OA), a natural terpenoid compound, effectively inhibited PM-induced skin aging; however, OA has poor water solubility and skin absorption, which limit its application in medicines and cosmetics. The aim of this study was to prepare oleanolic acid nanofibers (OAnf) and evaluate the effects of OA and OAnf in PM-treated keratinocytes. The results showed that OA dissolved in dissolved in dimethyl sulfoxide (DMSO) attenuated PM-induced reactive oxygen species overproduction, stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) activation, and the expressions of inflammatory and skin-aging-related proteins. In addition, the nanofiber process of OA effectively improved the water solubility of OA more than 99,000-fold through changing its physicochemical properties, including a surface area increase, particle size reduction, amorphous transformation, and hydrogen bonding formation with excipients. The skin penetration ability of OAnf was consistently over 10-fold higher than that of OA. Moreover, when dissolved in PBS, OAnf displayed superior antioxidant, anti-inflammatory, and anti-skin aging activities in PM-treated keratinocytes than OA. In conclusion, our findings suggest that OAnf could be a topical antioxidant formulation to attenuate skin problems caused by PM.

scholarly journals Can Plant Phenolic Compounds Protect the Skin from Airborne Particulate Matter?

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 379 ◽  
Author(s):  
Yong Chool Boo
Keyword(s):  
Particulate Matter ◽  
Phenolic Compounds ◽  
Skin Diseases ◽  
Inflammatory Responses ◽  
Acne Vulgaris ◽  
Experimental Studies ◽  
Airborne Particulate Matter ◽  
Skin Aging ◽  
Atmospheric Environment ◽  
Airborne Particulate

The skin is directly exposed to the polluted atmospheric environment, and skin diseases, such as atopic dermatitis and acne vulgaris, can be induced or exacerbated by airborne particulate matter (PM). PM can also promote premature skin aging with its accompanying functional and morphological changes. PM-induced skin diseases and premature skin aging are largely mediated by reactive oxygen species (ROS), and the harmful effects of PM may be ameliorated by safe and effective natural antioxidants. Experimental studies have shown that the extracts and phenolic compounds derived from many plants, such as cocoa, green tea, grape, pomegranate, and some marine algae, have antioxidant and anti-inflammatory effects on PM-exposed cells. The phenolic compounds can decrease the levels of ROS in cells and/or enhance cellular antioxidant capacity and, thereby, can attenuate PM-induced oxidative damage to nucleic acids, proteins, and lipids. They also lower the levels of cytokines, chemokines, cell adhesion molecules, prostaglandins, and matrix metalloproteinases implicated in cellular inflammatory responses to PM. Although there is still much research to be done, current studies in this field suggest that plant-derived phenolic compounds may have a protective effect on skin exposed to high levels of air pollution.

scholarly journals Electrospun Resveratrol-Loaded Polyvinylpyrrolidone/Cyclodextrin Nanofibers and Their Biomedical Applications

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 552 ◽  
Author(s):  
Ying-Cheng Lin ◽  
Stephen Chu-Sung Hu ◽  
Pao-Hsien Huang ◽  
Tzu-Ching Lin ◽  
Feng-Lin Yen
Keyword(s):  
Antioxidant Activity ◽  
Physicochemical Properties ◽  
Water Solubility ◽  
Skin Penetration ◽  
Aqueous Solubility ◽  
Amorphous Structure ◽  
Penetration Ability ◽  
Potential Applications ◽  
Anti Inflammatory ◽  
Franz Diffusion Cells

Resveratrol is a naturally occurring polyphenol compound which has been shown to possess antioxidant and anti-inflammatory properties. However, its pharmaceutical applications are limited by its poor water solubility. In this study, we used electrospinning technology to synthesize nanofibers of polyvinylpyrrolidone (PVP) and hydroxypropyl-β-cyclodextrin (HPBCD) loaded with resveratrol. We used X-ray diffractometry to analyze crystalline structure, Fourier transform infrared spectroscopy to determine intermolecular hydrogen bonding, antioxidant assays to measure antioxidant activity, and Franz diffusion cells to evaluate skin penetration. Our results showed that the aqueous solubility of resveratrol nanofibers was greatly improved (by more than 20,000-fold) compared to the pure compound. Analysis of physicochemical properties demonstrated that following nanofiber formation, resveratrol was converted from a crystalline to amorphous structure, and resveratrol formed new intermolecular bonds with PVP and HPBCD. Moreover, resveratrol nanofibers showed good antioxidant activity. In addition, the skin penetration ability of resveratrol in the nanofiber formulation was greater than that of pure resveratrol. Furthermore, resveratrol nanofibers suppressed particulate matter (PM)-induced expression of inflammatory proteins (COX-2 and MMP-9) in HaCaT keratinocytes. Therefore, resveratrol-loaded nanofibers can effectively improve the solubility and physicochemical properties of resveratrol, and may have potential applications as an antioxidant and anti-inflammatory formulation for topical skin application.

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