scholarly journals Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Acacia Seyal Gum Using Response Surface Methodology and Their Chemical Content Identification by Raman, FTIR, and GC-TOFMS

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1612
Author(s):  
Tahani Maher ◽  
Nassereldeen A. Kabbashi ◽  
Mohamed E. S. Mirghani ◽  
Md Z. Alam ◽  
Djabir Daddiouaissa ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Bioactive Compounds ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Acacia Seyal

Acacia Seyal gum (ASG), also known as gum Arabic, is an antioxidant-rich soluble fiber. ASG has been reported to have many biological activities, including anticancer, antidiabetic, antiulcer, and immunomodulatory activity. Extraction of bioactive compounds from ASG is commonly performed using conventional extraction methods. However, these techniques have certain limitation in terms of extraction time, energy, and solvent requirements. Ultrasound-assisted extraction (UAE) could be used as an alternative technique to extract bioactive compounds in less time, at low temperature, and with less energy and solvent requirements. In this study, the UAE extraction of ASG was optimized using response surface methodology (RSM). A face-centered central composite design (FCCCD) was used to monitor the effect of different independent factors of ultrasound operation (sonication time, temperature, and solvent ratio) on ASG extraction yield. In addition, screening and characterization of phytochemicals in 60% ethanol ASG extract was carried out using Raman microscopy, Fourier transform infrared spectroscopy (FTIR), and gas chromatography time-of-flight mass spectroscopy (GC-TOFMS) analysis. The results indicated that, under optimal conditions (extraction time 45 min, extraction temperature 40 °C, and solid–liquid ratio of 1:25 g/mL), the yield of ASG was 75.87% ± 0.10. This yield was reasonably close to the predicted yield of 75.39% suggested by the design of experiment. The ANOVA revealed that the model was highly significant due to the low probability value (p < 0.0001). Raman spectrum fingerprint detected polysaccharides, such as galactose and glucose, and protein like lysine and proline, while FTIR spectrum revealed the presence of functional groups peaks value of alkanes, aldehydes, aliphatic amines, and phenol. GC-TOFMS spectroscopic detected the presence of strong d-galactopyranose, carotenoid, and lycopene antioxidant compounds. In conclusion, this study demonstrated that the UAE technique is an efficient method to achieve a high yield of ASG extracts. The selected model is adequate to optimize the extraction of several chemical compounds reported in this study.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolics from Sideritis raeseri Using Response Surface Methodology

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3949
Author(s):  
Katarina Šavikin ◽  
Jelena Živković ◽  
Teodora Janković ◽  
Nada Ćujić-Nikolić ◽  
Gordana Zdunić ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In this study we define the optimal conditions for ultrasound-assisted extraction of bioactive polyphenols from S. raeseri aerial parts using response surface methodology. The influence of ethanol concentration (10–90%), extraction temperature (20–80 °C), extraction time (10–60 min), and solid-to-solvent ratio (1:10–1:50) on total phenolic content as well as on content of individual flavonoids, and hypolaetin and isoscutellarein derivatives was studied. For the experimental design, a central composite design was chosen. In the obtained extracts, the following ranges of targeted compounds were detected: total phenol from 19.32 to 47.23 mg GAE/g dw, HYP from 1.05 to 11.46 mg/g dw, ISC 1 from 0.68 to 10.68 mg/g dw, and ISC 2 from 0.74 to 15.56 mg/g dw. The optimal extraction conditions were set as: ethanol concentration of 65%, extraction time of 50 min, extraction temperature of 63 °C, and solid-to-solvent ratio of 1:40. Contents of TP, HYP, ISC 1, and ISC 2 in optimal extracts were 47.11 mg GAE/g dw, 11.73 mg/g dw, 9.54 mg/g dw, and 15.40 mg/g dw, respectively. Experimentally set values were in good agreement with those predicted by the response surface methodology model, indicating suitability of the used model, as well as the success of response surface methodology in optimizing the conditions of the extraction.

scholarly journals Response Surface Methodology (RSM)-Based Optimization of Ultrasound-Assisted Extraction of Sennoside A, Sennoside B, Aloe-Emodin, Emodin, and Chrysophanol from Senna alexandrina (Aerial Parts): HPLC-UV and Antioxidant Analysis

Molecules ◽  
2022 ◽  
Vol 27 (1) ◽  
pp. 298
Author(s):  
Perwez Alam ◽  
Omar M. Noman ◽  
Rashed N. Herqash ◽  
Omer M. Almarfadi ◽  
Ali Akhtar ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Aerial Parts ◽  
Assisted Extraction ◽  
Aloe Emodin ◽  
Ultrasound Assisted

In this study, ultrasound-assisted extraction conditions were optimized to maximize the yields of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol from S. alexandrina (aerial parts). The three UAE factors, extraction temperature (S1), extraction time (S2), and liquid to solid ratio (S3), were optimized using response surface methodology (RSM). A Box–Behnken design was used for experimental design and phytoconstituent analysis was performed using high-performance liquid chromatography-UV. The optimal extraction conditions were found to be a 64.2 °C extraction temperature, 52.1 min extraction time, and 25.2 mL/g liquid to solid ratio. The experimental values of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol (2.237, 12.792, 2.457, 0.261, and 1.529%, respectively) agreed with those predicted (2.152, 12.031, 2.331, 0.214, and 1.411%, respectively) by RSM models, thus demonstrating the appropriateness of the model used and the accomplishment of RSM in optimizing the extraction conditions. Excellent antioxidant properties were exhibited by S. alexandrina methanol extract obtained using the optimized extraction conditions with a DPPH assay (IC50 = 59.7 ± 1.93, µg/mL) and ABTS method (47.2 ± 1.40, µg/mL) compared to standard ascorbic acid.

Process Optimization of Ultrasound-Assisted Extraction of Pectin from Helianthus annuus L. Heads by Response Surface Methodology

2014 ◽  
Vol 955-959 ◽  
pp. 848-854
Author(s):  
Yin Xiang Gao ◽  
Lei Yang ◽  
Yuan Gang Zu ◽  
Li Ping Yao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Helianthus Annuus ◽  
Response Surfaces ◽  
Quadratic Model ◽  
High Yield ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

An ultrasound-assisted procedure for the extraction of pectin from heads ofHelianthus annuusL. (sunflower) was established. A Box–Behnken design (BBD) was employed to optimize the extraction temperature (X1: 30–50°C), extraction time (X2: 20–40 min) and pH (X3: 2.5–3.5) to obtain a high yield of pectin with high degree of esterification (DE) from sunflower heads. Analysis of variance showed that the contribution of a quadratic model was significant for the pectin extraction yield and DE. An optimization study using response surface methodology was performed and 3D response surfaces were plotted from the mathematical model. According to the RSM model, the highest pectin yield (23.11 ± 0.08%) and DE (39.85 ± 0.14%) can be achieved when the UAE process is carried out at 50°C for 40min using a hydrochloric acid solution of pH 3.0. These results suggest that ultrasound-assisted extraction could be a good option for the extraction of functional pectin from sunflower heads at industrial level.

scholarly journals Modelling and Optimization of Ultrasound Assisted Extraction of Polyphenols Using Response Surface Methodology

2020 ◽  
Vol 5 (9) ◽  
pp. 1004-1012
Author(s):  
Junior Franck Ekorong Akouan Anta ◽  
Biloa Dorothée Marcelle ◽  
Bruno Fabrice Siewe ◽  
Raghavarao K. S. M. S.
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Reducing Power ◽  
Extraction Yield ◽  
Operating Conditions ◽  
Extraction Time ◽  
Ultrasound Assisted Extraction ◽  
Stirring Rate ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Mango seed kernels are by-products of the consumption and transformation of mango fruits (Mangifera indica L.). Many ways of valorisation have been proposed, and among them, their phenolic compounds extraction. To increase the extraction yield, ultrasound-assisted extraction was modelled and optimized. The 4 factors Central Composite design associated with the Response Surface Methodology (RSM) were used to achieve that goal. The effect of extraction time, temperature, stirring rate and the Ultrasound Amplitude, on the total phenolic compound extraction yield and the total reducing power of the extract, were studied and modelled. The modelling allows us to do a multi-response optimization to identify the best-operating conditions to achieve at the same time the highest extraction yield and antioxidant capacity. The optimal operating conditions achieved were 41.82 min of extraction time, 54.75⁰C as extraction time, under 266.67 rpm as stirring rate, and 100% ultrasound amplitude. With an expected extraction yield of 71.35 mg GA/g, and 123.058 mg AA/g of total reducing power. 2 extraction cycles, under these conditions, are enough to extract a maximum of the phenolic content, under the described conditions.

scholarly journals Critical Variables Influencing the Ultrasound-Assisted Extraction of Bioactive Compounds—A Review

2021 ◽  
Vol 5 (1) ◽  
pp. 50
Author(s):  
Anxo Carreira-Casais ◽  
Maria Carpena ◽  
Antia G. Pereira ◽  
Franklin Chamorro ◽  
Anton Soria-Lopez ◽  
...  
Keyword(s):  
Green Chemistry ◽  
Bioactive Compounds ◽  
Extraction Methods ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Extraction Performance ◽  
Ultrasound Power

Ultrasound-assisted extraction (UAE) is a novel methodology, belonging to the so-called “Green Chemistry”, which has gained interest in recent years due to the potential to recover bioactive compounds, especially those from plant matrices. It is widely recognized that the extraction of molecules by UAE gives rise to higher or similar yields than those obtained by traditional extraction methods. UAE has certain advantages inherent to Green Chemistry extraction methods, such as short extraction time and low solvent consumption. The aim of this review is to critically present the different variables and parameters that can be modified in UAE, such as ultrasound power, time, temperature, solvent, and solid to solvent ratio that influence yield and extraction performance.

scholarly journals Chemical composition of dried Stevia rebaudiana Bertoni leaves and effect of ultrasound-assisted extraction on total steviosides content in extract

2021 ◽  
Vol 67 (1) ◽  
pp. 1-7
Author(s):  
Nguyen Thi Hoang Yen ◽  
Le Pham Tan Quoc
Keyword(s):  
Colorimetric Assay ◽  
Stevia Rebaudiana ◽  
Extraction Time ◽  
Chemical Compositions ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Major Chemical

Summary Introduction: Steviol stevioside, which has been used in the production of food products as a low-calorie sweeten, is one of the main glycoside groups in the leaves of the Stevia plants. It is useful for human health. Objective: The main objection of the present study was to find out some major chemical compositions of the dried Stevia rebaudiana Bertoni leaves and an effective, affordable, and environmentally friendly method to reach the high extraction yield of total steviosides from them. Therefore, a novel extraction, ultrasound-assisted extraction (UAE), was carried out to extract total steviosides from dried leaves of S. rebaudiana plant with ethanol of 70% (v/v) as a solvent. Methods: Some major chemical compositions of the dried S. rebaudiana leaves were analyzed according to the AOAC (Association of Official Analytical Chemists) and total steviosides content (TSC) was measured by anthrone-sulphuric acid colorimetric assay with main influencing factors including material/solvent ratio, extraction temperature, and extraction time. Results: The results referred that some chemical compounds such as protein, lipid, fibre, sugar, etc. existed in this material. The best extraction conditions were the sample/solvent ratio of 1:100 (g/ml), extraction temperature of 75oC, and extraction time of 30 min. Conclusion: The highest amount of total steviosides content of 8.894 % was obtained at the optimal extraction condition. Consequently, these results demonstrated that the parameters of UAE were applied successfully for producing total glycosides.

scholarly journals Response Surface Optimization of a Rapid Ultrasound-Assisted Extraction Method for Simultaneous Determination of Tetracycline Antibiotics in Manure

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Lanqing Li ◽  
Mingxing Sun ◽  
Hui Zhou ◽  
Yun Zhou ◽  
Ping Chen ◽  
...  
Keyword(s):  
Response Surface ◽  
Extraction Method ◽  
Extraction Procedure ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Ultrasound Assisted Extraction ◽  
Solution Ph ◽  
Assisted Extraction ◽  
Ultrasound Assisted

A rapid and cleanup-free ultrasound-assisted extraction method is proposed for the simultaneous extraction of oxytetracycline, tetracycline, chlortetracycline, and doxycycline in manure. The analytes were determined using high-performance liquid chromatography with ultraviolet detector. The influence of several variables on the efficiency of the extraction procedure was investigated by single-factor experiments. The temperature, pH, and amount of extraction solution were selected for optimization experiment using response surface methodology. The calibration curves showed good linearity (R2>0.99) for all analytes in the range of 0.1–20 μg/mL. The four antibiotics were successfully extracted from manure with recoveries ranging from 81.89 to 92.42% and good reproducibility (RSD, <4.06%) under optimal conditions, which include 50 mL of McIlvaine buffer extraction solution (pH 7.15) mixed with 1 g of manure sample, extraction temperature of 40°C, extraction time of 10 min, and three extraction cycles. Method quantification limits of 1.75–2.32 mg/kg were obtained for the studied compounds. The proposed procedure demonstrated clear reductions in extraction time and elimination of cleanup steps. Finally, the applicability to tetracyclines antibiotics determination in real samples was evaluated through the successful determination of four target analytes in swine, cow manure, and mixture of animal manure with inorganic fertilizer.

Optimization of Ultrasonic-Assisted Extraction for Pinocembrin from Flos populi Using Response Surface Methodology

2017 ◽  
Vol 13 (5) ◽  
Author(s):  
Zunlai Sheng ◽  
Biying Wang ◽  
Jiahong Zhao ◽  
Wenhui Yu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Surface Model ◽  
Extraction Temperature ◽  
Acoustic Intensity ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasonic Assisted

Abstract In this study, the ultrasound-assisted extraction (UAE) was used for extraction of pinocembrin from Flos populi. Based on the results of the single-factor experiment, four independent parameters, including ethanol concentration (40–80 %), extraction temperature (50–70 °C), extraction time (25–45 min) and electrical acoustic intensity (40.8–81.5 W/m2) were further investigated using response surface methodology (RSM) coupled with Box-Behnken design. The experimental data were fitted to the quadratic response surface model using multiple regression analysis with high adjusted determination coefficient value (R 2) of 0.9697. The highest yield (134.2 ± 1.53 mg/g) of pinocembrin was obtained under the optimal conditions (ethanol concentration of 68 %, extraction temperature of 69 °C, extraction time of 42 min and electrical acoustic intensity of 66.81 W/cm2), which agreed to the predicted value of 132.9 mg/g. Moreover, the comparison between the UAE and reflux extraction also showed the suitability of UAE for pinocembrin from Flos populi.

Sign in / Sign up

Export Citation Format

Share Document