scholarly journals The Effectiveness of Nafion-Coated Stainless Steel Surfaces for Inhibiting Bacillus Subtilis Biofilm Formation

2020 ◽  
Vol 10 (14) ◽  
pp. 5001
Author(s):  
Lijuan Zhong ◽  
Yibo Song ◽  
Shufeng Zhou
Keyword(s):  
Stainless Steel ◽  
Bacillus Subtilis ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Stainless Steel Surface ◽  
Electrostatic Effects ◽  
Uncoated Surface ◽  
Coated Surface ◽  
Steel Surfaces

Stainless steel is one of most commonly used materials in the world; however, biofilms on the surfaces of stainless steel cause many serious problems. In order to find effective methods of reducing bacterial adhesion to stainless steel, and to investigate the role of electrostatic effects during the formation of biofilms, this study used a stainless steel surface that was negatively charged by being coated with Nafion which was terminated by sulfonic groups. The results showed that the roughness of stainless steel discs coated with 1% Nafion was similar to an uncoated surface; however the hydrophobicity increased, and the Nafion-coated surface reduced the adhesion of Bacillus subtilis by 75% compared with uncoated surfaces. Therefore, a facile way to acquire antibacterial stainless steel was found, and it is proved that electrostatic effects have a significant influence on the formation of biofilms.

Recent Patents on Impact of Lipopeptide on the Biofilm Formation onto Titanium and Stainless Steel Surfaces

2020 ◽  
Vol 14 (1) ◽  
pp. 49-62
Author(s):  
Mauro Ezio Eustáquio Pires ◽  
Adriano Guimarães Parreira ◽  
Tuânia Natacha Lopes Silva ◽  
Heloísa Carneiro Colares ◽  
José Antonio da Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Stainless Steel ◽  
Bacillus Subtilis ◽  
Biofilm Formation ◽  
Orthopedic Implants ◽  
Stainless Steel Surface ◽  
E Coli ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Steel Surfaces

Background: Numerous causes of infection in arthroplasties are related to biofilm formation on implant surfaces. In order to circumvent this problem, new alternatives to prevent bacterial adhesion biosurfactants-based are emerging due to low toxicity, biodegradability and antimicrobial activity of several biosurfactants. We revised all patents relating to biosurfactants of applicability in orthopedic implants. Methods: This work aims to evaluate the capability of a lipopeptide produced by Bacillus subtilis ATCC 19659 isolates acting as inhibitors of the adhesion of Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 onto titanium and stainless steel surfaces and its antimicrobial activity. Results: The adhesion of the strains to the stainless-steel surface was higher than that of titanium. Preconditioning of titanium and stainless-steel surfaces with 10 mg mL-1 lipopeptide reduced the adhesion of E. coli by up to 93% and the adhesion of S. aureus by up to 99.9%, suggesting the strong potential of lipopeptides in the control of orthopedic infections. The minimal inhibitory concentration and minimum bactericidal concentration were 10 and 240 µg mL-1 for E. coli and S. aureus, respectively. Conclusion: The lipopeptide produced by Bacillus subtilis ATCC 19659 presented high biotechnological application in human health against orthopedic implants infections.

scholarly journals N-Acetyl-l-Cysteine Affects Growth, Extracellular Polysaccharide Production, and Bacterial Biofilm Formation on Solid Surfaces

2003 ◽  
Vol 69 (8) ◽  
pp. 4814-4822 ◽  
Author(s):  
Ann-Cathrin Olofsson ◽  
Malte Hermansson ◽  
Hans Elwing
Keyword(s):  
Stainless Steel ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
Paper Mill ◽  
Bacterial Biofilm ◽  
Extracellular Polysaccharides ◽  
Positive Effects ◽  
Initial Adhesion ◽  
Interesting Candidate ◽  
Steel Surfaces

ABSTRACT N-Acetyl-l-cysteine (NAC) is used in medical treatment of patients with chronic bronchitis. The positive effects of NAC treatment have primarily been attributed to the mucus-dissolving properties of NAC, as well as its ability to decrease biofilm formation, which reduces bacterial infections. Our results suggest that NAC also may be an interesting candidate for use as an agent to reduce and prevent biofilm formation on stainless steel surfaces in environments typical of paper mill plants. Using 10 different bacterial strains isolated from a paper mill, we found that the mode of action of NAC is chemical, as well as biological, in the case of bacterial adhesion to stainless steel surfaces. The initial adhesion of bacteria is dependent on the wettability of the substratum. NAC was shown to bind to stainless steel, increasing the wettability of the surface. Moreover, NAC decreased bacterial adhesion and even detached bacteria that were adhering to stainless steel surfaces. Growth of various bacteria, as monocultures or in a multispecies community, was inhibited at different concentrations of NAC. We also found that there was no detectable degradation of extracellular polysaccharides (EPS) by NAC, indicating that NAC reduced the production of EPS, in most bacteria tested, even at concentrations at which growth was not affected. Altogether, the presence of NAC changes the texture of the biofilm formed and makes NAC an interesting candidate for use as a general inhibitor of formation of bacterial biofilms on stainless steel surfaces.

scholarly journals Fire Ant Venom Alkaloids Inhibit Biofilm Formation

Toxins ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 420 ◽  
Author(s):  
Danielle Bruno de Carvalho ◽  
Eduardo Gonçalves Paterson Fox ◽  
Diogo Gama dos Santos ◽  
Joab Sampaio de Sousa ◽  
Denise Maria Guimarães Freire ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Bacterial Adhesion ◽  
Solenopsis Invicta ◽  
Biofilm Formation ◽  
Food Industry ◽  
Fire Ant ◽  
Ant Venom ◽  
Steel Surfaces ◽  
Adhesion Inhibition ◽  
Medical Health

Biofilm formation on exposed surfaces is a serious issue for the food industry and medical health facilities. There are many proposed strategies to delay, reduce, or even eliminate biofilm formation on surfaces. The present study focuses on the applicability of fire ant venom alkaloids (aka ‘solenopsins’, from Solenopsis invicta) tested on polystyrene and stainless steel surfaces relative to the adhesion and biofilm-formation by the bacterium Pseudomonas fluorescens. Conditioning with solenopsins demonstrates significant reduction of bacterial adhesion. Inhibition rates were 62.7% on polystyrene and 59.0% on stainless steel surfaces. In addition, solenopsins drastically reduced cell populations already growing on conditioned surfaces. Contrary to assumptions by previous authors, solenopsins tested negative for amphipathic properties, thus understanding the mechanisms behind the observed effects still relies on further investigation.

scholarly journals Chemical sanitizers to control biofilms formed by two Pseudomonas species on stainless steel surface

2012 ◽  
Vol 32 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Danila Soares Caixeta ◽  
Thiago Henrique Scarpa ◽  
Danilo Florisvaldo Brugnera ◽  
Dieyckson Osvani Freire ◽  
Eduardo Alves ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stainless Steel ◽  
Biofilm Formation ◽  
Skim Milk ◽  
304 Stainless Steel ◽  
Stainless Steel Surface ◽  
Aisi 304 ◽  
Pseudomonas Species ◽  
Sodium Dichloroisocyanurate ◽  
Different Temperatures

The biofilm formation of Pseudomonas aeruginosa and Pseudomonas fluorescens on AISI 304 stainless steel in the presence of reconstituted skim milk under different temperatures was conducted, and the potential of three chemical sanitizers in removing the mono-species biofilms formed was compared. Pseudomonas aeruginosa cultivated in skim milk at 28 °C presented better growth rate (10.4 log CFU.mL-1) when compared with 3.7 and 4.2 log CFU.mL-1 for P. aeruginosa and P. fluorescens cultivated at 7 °C, respectively. Pseudomonas aeruginosa formed biofilm when cultivated at 28 °C. However, only the adhesion of P. aeruginosa and P. fluorescens was observed when incubated at 7 °C. The sodium dichloroisocyanurate was the most efficient sanitizer in the reduction of the adhered P. aeruginosa cells at 7 and 28 °C and those on the biofilm, respectively. The hydrogen peroxide was more effective in the reduction of adhered cells of P. fluorescens at 7 °C.

scholarly journals Impact of Marine Bacterial Adhesion on the Physico-chemical Properties of Stainless Steel Surfaces

2019 ◽  
Vol 13 (1) ◽  
pp. 50-57
Author(s):  
Douaae Ou-yahia ◽  
Kawtar Fikri-Benb ◽  
Yassir Lekbach ◽  
Fadoua Bennouna ◽  
Hassan Barkai ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Bacterial Adhesion ◽  
Chemical Properties ◽  
Physico Chemical ◽  
Steel Surfaces

Biofilm Formation and Sporulation by Bacillus cereus on a Stainless Steel Surface and Subsequent Resistance of Vegetative Cells and Spores to Chlorine, Chlorine Dioxide, and a Peroxyacetic Acid–Based Sanitizer

2005 ◽  
Vol 68 (12) ◽  
pp. 2614-2622 ◽  
Author(s):  
JEE-HOON RYU ◽  
LARRY R. BEUCHAT
Keyword(s):  
Stainless Steel ◽  
Relative Humidity ◽  
Bacillus Cereus ◽  
Chlorine Dioxide ◽  
Biofilm Formation ◽  
Total Cell ◽  
Stainless Steel Surface ◽  
Peroxyacetic Acid ◽  
Vegetative Cells ◽  
Cell Counts

Biofilm formation by Bacillus cereus 038-2 on stainless steel coupons, sporulation in the biofilm as affected by nutrient availability, temperature, and relative humidity, and the resistance of vegetative cells and spores in biofilm to sanitizers were investigated. Total counts in biofilm formed on coupons immersed in tryptic soy broth (TSB) at 12 and 22°C consisted of 99.94% of vegetative cells and 0.06% of spores. Coupons on which biofilm had formed were immersed in TSB or exposed to air with 100, 97, 93, or 85% relative humidity. Biofilm on coupons immersed in TSB at 12°C for an additional 6 days or 22°C for an additional 4 days contained 0.30 and 0.02% of spores, respectively, whereas biofilm exposed to air with 100 or 97% relative humidity at 22°C for 4 days contained 10 and 2.5% of spores, respectively. Sporulation did not occur in biofilm exposed to 93 or 85% relative humidity at 22°C. Treatment of biofilm on coupons that had been immersed in TSB at 22°C with chlorine (50 μg/ml), chlorine dioxide (50 μg/ml), and a peroxyacetic acid–based sanitizer (Tsunami 200, 40 μg/ml) for 5 min reduced total cell counts (vegetative cells plus spores) by 4.7, 3.0, and 3.8 log CFU per coupon, respectively; total cell counts in biofilm exposed to air with 100% relative humidity were reduced by 1.5, 2.4, and 1.1 log CFU per coupon, respectively, reflecting the presence of lower numbers of vegetative cells. Spores that survived treatment with chlorine dioxide had reduced resistance to heat. It is concluded that exposure of biofilm formed by B. cereus exposed to air at high relative humidity (≥97%) promotes the production of spores. Spores and, to a lesser extent, vegetative cells embedded in biofilm are protected against inactivation by sanitizers. Results provide new insights to developing strategies to achieve more effective sanitation programs to minimize risks associated with B. cereus in biofilm formed on food contact surfaces and on foods.

Nanoengineered Superhydrophobic Surfaces to Prevent Adhesion of Listeria monocytogenes for Improved Food Safety

2020 ◽  
Vol 63 (5) ◽  
pp. 1401-1407
Author(s):  
Bog Eum Lee ◽  
Youngsang You ◽  
Won Choi ◽  
Eun-mi Hong ◽  
Marisa M. Wall ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Biofilm Formation ◽  
Electrochemical Etching ◽  
Contact Angles ◽  
Bacterial Attachment ◽  
Superhydrophobic Surfaces ◽  
List Type ◽  
Ptfe Film ◽  
Steel Surfaces

HighlightsNanoporous superhydrophobic surfaces were fabricated using electrochemical etching and Teflon coating.Adhesion of Listeria monocytogenes to the nanoengineered stainless steel surfaces was reduced.Self-cleanable food-contact surfaces prevent bacterial attachment and subsequent biofilm formation.Abstract. Bacterial attachment on solid surfaces and subsequent biofilm formation is a significant problem in the food industry. Superhydrophobic surfaces have potential to prevent bacterial adhesion by minimizing the contact area between bacterial cells and the surface. In this study, stainless steel-based superhydrophobic surfaces were fabricated by manipulating nanostructures with electrochemical etching and polytetrafluoroethylene (PTFE) film. The formation of nanostructures on stainless steel surfaces was characterized by field emission scanning electron microscopy (FESEM). The stainless steel surfaces etched at 10 V for 5 min and at 10 V for 10 min with PTFE deposition resulted in average water contact angles of 154° ±4° with pore diameters of 50 nm. In addition, adhesion of Listeria monocytogenes was decreased by up to 99% compared to the bare substrate. These findings demonstrate the potential for the development of antibacterial surfaces by combining nanoporous patterns with PTFE films. Keywords: Electrochemical etching, PTFE, Nanoengineered surface, L. monocytogenes, Superhydrophobic.

Destruction of Alicyclobacillus acidoterrestris Spores in Apple Juice on Stainless Steel Surfaces by Chemical Disinfectants

2009 ◽  
Vol 72 (3) ◽  
pp. 510-514 ◽  
Author(s):  
RICHARD PODOLAK ◽  
PHILIP H. ELLIOTT ◽  
BRADLEY J. TAYLOR ◽  
AAKASH KHURANA ◽  
DARRYL G. BLACK
Keyword(s):  
Stainless Steel ◽  
Chlorine Dioxide ◽  
Apple Juice ◽  
Octanoic Acid ◽  
Stainless Steel Surface ◽  
Peroxyacetic Acid ◽  
Alicyclobacillus Acidoterrestris ◽  
Practical Applications ◽  
Maximum Reduction ◽  
Steel Surfaces

A study was conducted to determine the effects of three commercially available disinfectants on the reduction of Alicyclobacillus acidoterrestris spores in single-strength apple juice applied to stainless steel surfaces. Apple juice was inoculated with A. acidoterrestris spores, spread onto the surface of stainless steel chips (SSC), dried to obtain spore concentrations of approximately 104 CFU/cm2, and treated with disinfectants at temperatures ranging from 40 to 90°C. The concentrations of disinfectants were 200, 500, 1,000, and 2,000 ppm of total chlorine for Clorox (CL) (sodium hypochlorite); 50, 100, and 200 ppm of total chlorine for Carnebon 200 (stabilized chlorine dioxide); and 1,500, 2,000, and 2,600 ppm for Vortexx (VOR) (hydrogen peroxide, peroxyacetic acid, and octanoic acid). For all temperatures tested, VOR at 2,600 ppm (90°C) and CL at 2,000 ppm (90°C) were the most inhibitory against A. acidoterrestris spores, resulting in 2.55- and 2.32-log CFU/cm2 reductions, respectively, after 2 min. All disinfectants and conditions tested resulted in the inactivation of A. acidoterrestris spores, with a maximum reduction of >2 log CFU/cm2. Results from this study indicate that A. acidoterrestris spores, in single-strength apple juice, may be effectively reduced on stainless steel surface by VOR and CL, which may have practical applications in the juice industry.

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