Rabbit as an Aging Model in Reproduction: Advanced Maternal Age Alters GLO1 Expression in the Endometrium at the Time of Implantation

Advanced maternal age is associated with adverse pregnancy outcomes and the decline of female fertility in mammals. A potential reason for reduced fertility is metabolic changes due to protein modifications by advanced glycation end products. To elucidate the aging process in female reproduction, we analysed a key enzyme for detoxification of reactive dicarbonyls, the glyoxalase 1 (GLO1), in reproductive organs and blastocysts of young and old rabbits at the preimplantation stage. At day 6 post coitum, uterine, oviductal, ovarian tissue and blastocysts from young (16–20 weeks) and old rabbits (>108 weeks) were characterised for GLO1 expression. GLO1 amounts, enzymatic activity and localisation were quantified by qPCR, Simple Western, activity assay and immunohistochemistry. The GLO1 enzyme was present and active in all reproductive tract organs in a cell-type-specific pattern. Ovarian follicle and uterine epithelial cells expressed GLO1 to a high extent. In tertiary follicles, GLO1 expression increased, whereas it decreased in the endometrium of old rabbits at day 6 of pregnancy. In blastocysts of old animals, GLO1 expression remained unchanged. In early pregnancy, advanced maternal age leads to modified GLO1 expression in ovarian follicles and the endometrium, indicating an altered metabolic stress response at the preimplantation stage in older females.

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Advanced maternal age and adverse pregnancy outcomes: A cohort study

Taiwanese Journal of Obstetrics and Gynecology ◽

10.1016/j.tjog.2020.11.018 ◽

2021 ◽

Vol 60 (1) ◽

pp. 119-124

Author(s):

M. Guarga Montori ◽

A. Álvarez Martínez ◽

C. Luna Álvarez ◽

N. Abadía Cuchí ◽

P. Mateo Alcalá ◽

...

Keyword(s):

Cohort Study ◽

Pregnancy Outcomes ◽

Maternal Age ◽

Advanced Maternal Age ◽

Adverse Pregnancy Outcomes ◽

Adverse Pregnancy

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The implications for advanced maternal age on pregnancy complications and adverse neonatal outcomes

Perinatal Journal ◽

10.2399/prn.21.0293005 ◽

2021 ◽

Vol 29 (3) ◽

pp. 200-209

Author(s):

Zeynep Gedik Özköse ◽

Süleyman Cemil Oğlak

Keyword(s):

Birth Weight ◽

Cesarean Section ◽

Pregnancy Outcomes ◽

Maternal Age ◽

Advanced Maternal Age ◽

Neonatal Outcomes ◽

Apgar Scores ◽

Adverse Neonatal Outcomes ◽

Adverse Pregnancy ◽

Nicu Admission

Objective This study aimed to determine the effect of advanced maternal age (AMA) on maternal and neonatal outcomes in pregnant women aged ≥35 years compared with patients aged 30–34 years. Also, we aimed to analyze the risk estimates of potential confounders to identify whether these variables contributed to the development of adverse pregnancy outcomes or not. Methods This retrospective cohort study included 2284 pregnant women aged ≥35 years at the time of delivery who was delivered in a tertiary referral hospital from January 1, 2016, to December 31, 2020. We further classified these women into two subgroups: 35–39 years as early AMA (EAMA), and ≥40 years as very AMA (VAMA). Pregnancy complications and adverse neonatal outcomes were recorded. Results Compared to younger women, pregnant AMA women had significantly higher risks of complicated pregnancies, including a higher risk of gestational diabetes mellitus (GDM, p<0.001), polyhydramnios (p<0.001), cesarean section (p<0.001), stillbirths (p<0.001), major fetal abnormality (p<0.001), preterm delivery (p<0.001), lower birth weight (p<0.001), lower 5-minute Apgar scores (p<0.001), lower umbilical artery blood pH values (p<0.001), neonatal intensive care unit (NICU) admission (p<0.001), and length of NICU stay (p<0.001). Conclusion We found a strong and significant association between VAMA and adverse pregnancy outcomes, including an increased risk of GDM, polyhydramnios, cesarean section, and adverse neonatal outcomes, including a higher risk of stillbirths, preterm delivery, lower birth weight, lower 5-minute Apgar scores, and NICU admission.

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Risk of Adverse Pregnancy Outcomes at Advanced Maternal Age

Obstetrics and Gynecology ◽

10.1097/aog.0000000000002504 ◽

2018 ◽

Vol 131 (3) ◽

pp. 457-463 ◽

Cited By ~ 34

Author(s):

Line Elmerdahl Frederiksen ◽

Andreas Ernst ◽

Nis Brix ◽

Lea Lykke Braskhøj Lauridsen ◽

Laura Roos ◽

...

Keyword(s):

Pregnancy Outcomes ◽

Maternal Age ◽

Advanced Maternal Age ◽

Adverse Pregnancy Outcomes ◽

Adverse Pregnancy

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Risks of Selected Fetal Adverse Pregnancy Outcomes at Advance Maternal Age

10.53350/pjmhs211582468 ◽

2021 ◽

Vol 15 (8) ◽

pp. 2468-2471

Author(s):

Saadia Yasmeen ◽

Sumayya . ◽

Javeria Saleem ◽

Jawairiah Liaqat ◽

Nadia Pervaiz ◽

...

Keyword(s):

Birth Weight ◽

Preterm Birth ◽

Low Birth Weight ◽

Pregnancy Outcomes ◽

Maternal Age ◽

Advanced Maternal Age ◽

Adverse Pregnancy Outcomes ◽

Control Group ◽

Postpartum Women ◽

Adverse Pregnancy

Background and Aim: Advanced maternal age pregnancy could be referred to as pregnancy after 35 years or older. The prevalence of postponing pregnancies is increasing day by day worldwide. However, limited evidence was found on advanced maternal age pregnancy association with fetal adverse outcomes. The present study aimed to evaluate the frequency of advanced maternal age selected fetal adverse pregnancy outcomes. Place and Duration: Obstetrics & Gynaecology department of Alkhidmat Hospital Kohat and Qazi Hussain Ahmad Medical Complex, Nowshera for duration of six months from November 2020 to April 2021. Materials and Methods: This single-centered retrospective study was conducted on 220 postpartum women in the study group (≥35 years) and 170 control group postpartum women (20-34 years) who delivered at Obstetrics & Gynaecology department of Alkhidmat Hospital Kohat and Qazi Hussain Ahmad Medical Complex, Nowshera after 28 gestational weeks. Individuals who met the inclusion criteria were enrolled and sampled based on randomized control sampling technique for both control and study groups. Data extraction checklist and pretested questionnaire were used for data collection from the maternal charts. Adverse pregnancy outcome and advanced maternal age was correlated adjusted relative risks and strength with a 95% confidence interval. SPSS version 20 was used for data analysis and considered p-value >0.05 as a statistical standard. Results: The adverse neonatal outcomes include low birth weight, preterm birth, and stillbirth with a prevalence 38 (17.4%), 46 (20.8%), and 31 (14.2%) respectively in the study group. In the control group, the incidence of low birth weight, preterm birth, and stillbirth was 21 (12.6%), 15 (14.6%) and 6 (3.5%) respectively. The stillbirth (ARR=3.16 95% CI (1.29–6.03) and preterm deliveries (ARR=2.71 95% CI (1.79–3.86) risk had significantly higher prevalence compared to control group advanced age pregnancy. Insignificance association was found between low birth weight and advanced maternal age. Conclusion: Stillbirth and preterm birth was the adverse fetal outcome significantly related with advanced maternal age pregnancy. There was no significant association between low birth weight and advanced maternal age. Keywords: Maternal age, Fetal Adverse Pregnancy Outcomes

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Risk of Adverse Pregnancy Outcomes at Advanced Maternal Age

Obstetric Anesthesia Digest ◽

10.1097/01.aoa.0000542378.64975.41 ◽

2018 ◽

Vol 38 (3) ◽

pp. 155

Author(s):

L.E. Frederiksen ◽

A. Ernst ◽

N. Brix ◽

L.L. Braskhøj Lauridsen ◽

L. Roos ◽

...

Keyword(s):

Pregnancy Outcomes ◽

Maternal Age ◽

Advanced Maternal Age ◽

Adverse Pregnancy Outcomes ◽

Adverse Pregnancy

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Advanced Maternal Age and Adverse Pregnancy Outcome: Evidence from a Large Contemporary Cohort

PLoS ONE ◽

10.1371/journal.pone.0056583 ◽

2013 ◽

Vol 8 (2) ◽

pp. e56583 ◽

Cited By ~ 232

Author(s):

Louise C. Kenny ◽

Tina Lavender ◽

Roseanne McNamee ◽

Sinéad M. O’Neill ◽

Tracey Mills ◽

...

Keyword(s):

Pregnancy Outcome ◽

Maternal Age ◽

Adverse Pregnancy Outcome ◽

Advanced Maternal Age ◽

Adverse Pregnancy

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A Prospective Cohort Study providing Insights for Markers of Adverse Pregnancy Outcome in Women of Advanced Maternal Age

10.21203/rs.3.rs-130070/v1 ◽

2020 ◽

Author(s):

Samantha Lean ◽

Rebecca Jones ◽

Stephen Roberts ◽

Alexander Heazell

Keyword(s):

Oxidative Stress ◽

Pregnancy Outcome ◽

Maternal Age ◽

Adverse Outcome ◽

Adverse Pregnancy Outcome ◽

Advanced Maternal Age ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Adverse Pregnancy ◽

And Oxidative Stress

Abstract Background Advanced maternal age (AMA; ≥35 years) is associated with increased rates of adverse pregnancy outcome. Better understanding of underlying pathophysiological processes may improve identification of AMA mothers who are at greatest risk of adverse outcome. This study aimed to investigate changes in oxidative stress and inflammation in AMA women and identify clinical and biochemical predictors of adverse pregnancy outcome in women of AMA.Methods The Manchester Advanced Maternal Age Study (MAMAS) was a multicentre, observational, prospective cohort study of 527 mothers. Participants were divided into three age groups for comparison 20-30 years (n=158), 35-39 years (n=212) and ≥40 years (n=157). Demographic and medical data were collected along with maternal blood samples at 28 and 36 weeks’ gestation. Multivariable analysis was conducted to identify variables associated with adverse outcome, defined as one or more of: small for gestational age (<10th centile), FGR (<5th centile), stillbirth, NICU admission, preterm birth <37 weeks gestation or Apgar score <7 at 5 minutes. Biomarkers of inflammation, oxidative stress and placental dysfunction were quantified in maternal serum. Univariate and multivariable statistical analyses were used to identify associations with composite adverse fetal outcome.Results: Maternal smoking was associated with adverse outcome in older mothers (Adjusted Odds Ratio (AOR) 4.34, 95% Confidence Interval (95%CI) 1.88, 9.99), whereas multiparity reduced the odds (AOR 0.56, 95% CI 0.34, 0.99). In uncomplicated AMA pregnancies, lower circulating anti-inflammatory IL-10, IL-RA and increased antioxidant capacity (TAC) were seen. In AMA with adverse outcome, TAC and oxidative stress markers were increased and levels of maternal circulating placental hormones (hPL, PlGF and sFlt-1) were reduced (p<0.05). Of these, placental growth factor had the strongest predictive accuracy (Area Under the Receiver Operator Characteristic (AUROC) = 0.74) followed by TAC (AUROC=0.69).Conclusions: This study identified alterations in circulating inflammatory and oxidative stress markers in AMA women and in AMA women with adverse pregnancy outcome providing preliminary evidence of mechanistic links. Further, larger studies are required to determine if these markers can be developed into a predictive model of an individual AMA woman’s risk of APO, enabling a reduction in stillbirth rates whilst minimising unnecessary intervention.

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Study of pregnancy outcome in elderly gravida

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20175247 ◽

2017 ◽

Vol 6 (12) ◽

pp. 5384

Author(s):

Pratibha V. Dixit ◽

Madhuri A. Mehendale

Keyword(s):

Maternal Age ◽

Pregnancy Termination ◽

Later Life ◽

Advanced Maternal Age ◽

Health Concern ◽

Demographic Shift ◽

Utmost Care ◽

Adverse Pregnancy ◽

Nicu Admission ◽

And Control

Background: Elderly gravida which is defined as age of mother as 35 years or more at the time of delivery has become increasingly common in last two to three decades. This accelerating demographic shift is of major clinical and public health concern, because advanced maternal age has consistently been associated with adverse pregnancy outcomes.Methods: An observational prospective study consisting of 120 pregnant women. All participants were divided into Study group and Control group.Results: Women of advanced maternal age have higher frequency of antenatal complications leading to early pregnancy termination and operative interference. There is significant increase in the incidence of adverse perinatal outcome due to preterm deliveries and NICU admission and neonatal death.Conclusions: With improved health services and better prospects of women who are >35 years of becoming pregnant, these pregnancies are fraught with complications. Women should realistically appraise the risks of pregnancy in later life. Hence these women should be counselled, and their complications managed with utmost care.

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