scholarly journals Effects of Black Jade on Osteogenic Differentiation of Adipose Derived Stem Cells under Benzopyrene

2021 ◽  
Vol 11 (3) ◽  
pp. 1346
Author(s):  
Yoonjin Park ◽  
Gyeong Hee Shin ◽  
Gyo Sik Jin ◽  
Sungbae Jin ◽  
Boyong Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Blood Circulation ◽  
Osteoblast Differentiation ◽  
Research Evidence ◽  
Adipose Derived Stem Cells ◽  
Differentiation Markers ◽  
Dermal Cell ◽  
Dermal Cells ◽  
Apoptotic Mechanism

Jade, a popular gemstone symbolizing beauty, grace, and longevity, is known to improve blood circulation; however, scientific research evidence is still lacking. The effect of black jade extract on the expression levels of apoptotic and osteogenic genes was validated using qPCR and flow cytometry. In combination with the use of a fluorescence microscope, osteogenic differentiation and the stained osteocytes count were analyzed. Under the pressure of benzo(a)pyrene, dermal cell apoptosis was accelerated and the osteogenic differentiation of adipose-derived stem cells (ASCs) was suppressed; but black jade extract counteracted the effects. Through an anti-apoptotic mechanism, the extract suppressed the expression of apoptotic proteins Bax and cytochrome C to 9 and 4.8 times, respectively, compared to that in dermal cells exposed to benzo(a)pyrene. During osteogenic differentiation of ASCs, the extract enhanced their differentiation despite being exposed to benzo(a)pyrene, and the relative levels of the osteoblast differentiation markers osteoponin, osteocalcin, and sclerostin were 1.87, 2.54, and 3.9 times higher, respectively, than those in the conditioned medium by benzo(a)pyrene. These effects of the extract indicate that black jade extract is very useful when applied as a functional biomaterial.

scholarly journals Biological Functions of Let-7e-5p in Promoting the Differentiation of MC3T3-E1 Cells

2021 ◽  
Vol 9 ◽  
Author(s):  
Chunli Wang ◽  
Songcai Liu ◽  
Jiaxin Li ◽  
Yunyun Cheng ◽  
Zhaoguo Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Osteoblast Differentiation ◽  
Embryonic Stem ◽  
Adipose Derived Stem Cells ◽  
Biological Functions ◽  
Transcriptional Expression ◽  
Protein Levels ◽  
Insight Into

MicroRNAs let-7c and let-7f, two members of the let-7 family, were involved in regulating osteoblast differentiation and have an important role in bone formation. Let-7e-5p, which also belonged to the let-7 family, presented in the differentiation of adipose-derived stem cells and mouse embryonic stem cells. However, the role of let-7e-5p in osteoblast differentiation was unclear. Thus, this study aimed to elucidate the function of let-7e-5p in osteoblast differentiation and its mechanism. Firstly, we found that the let-7e-5p mimic promoted osteoblast differentiation but not the proliferation of MC3T3-E1 cells by positively regulating the expression levels of osteogenic-associated genes (RUNX2, OCN, OPN, and OSX), the activity of ALP, and formation of mineralized nodules. Moreover, we ascertained that the let-7e-5p mimic downregulated the post-transcriptional expression of SOCS1 by specifically binding to the 3′ untranslated region of SOCS1 mRNA. Also, let-7e-5p-induced SOCS1 downregulation increased the protein levels of p-STAT5 and IGF-1, which were both modulated by SOCS1 molecules. Furthermore, let-7e-5p abrogated the inhibition of osteogenic differentiation mediated by SOCS1 overexpression. Therefore, these results suggested that let-7e-5p regulated the differentiation of MC3T3-E1 cells through the JAK2/STAT5 pathway to upregulate IGF-1 gene expression by inhibiting SOCS1. These findings may provide a new insight into the regulatory role of let-7e-5p in osteogenic differentiation and imply the existence of a novel mechanism underlying let-7e-5p-mediated osteogenic differentiation.

scholarly journals MiR ‐149‐3p can improve the osteogenic differentiation of human adipose‐derived stem cells via targeting AKT1

2021 ◽  
Author(s):  
Ai‐Ning Lai ◽  
Rong Zhou ◽  
Bin Chen ◽  
Long Guo ◽  
Yu‐Ya Dai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Adipose Derived Stem Cells

Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro

2021 ◽  
Vol 71 ◽  
pp. 101506
Author(s):  
Zhijie Liang ◽  
Donglin Huang ◽  
Wenhai Nong ◽  
Jinping Mo ◽  
Dandan Zhu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dependent Manner ◽  
Adipose Derived Stem Cells ◽  
Platelet Rich Fibrin ◽  
Dose Dependent

Icariin promotes proliferation and osteogenic differentiation of rat adipose-derived stem cells by activating the RhoA-TAZ signaling pathway

2017 ◽  
Vol 88 ◽  
pp. 384-394 ◽  
Author(s):  
Yaping Ye ◽  
Xingzhi Jing ◽  
Na Li ◽  
Yingxing Wu ◽  
Bingbing Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Adipose Derived Stem Cells

scholarly journals Induction of Osteogenic Differentiation of Adipose Derived Stem Cells by Microstructured Nitinol Actuator-Mediated Mechanical Stress

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e51264 ◽  
Author(s):  
Sarah Strauß ◽  
Sonja Dudziak ◽  
Ronny Hagemann ◽  
Stephan Barcikowski ◽  
Malte Fliess ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Mechanical Stress ◽  
Adipose Derived Stem Cells

Tetrahedral framework nucleic acids regulate osteogenic differentiation potential of osteoporotic adipose-derived stem cells

2021 ◽  
Author(s):  
Tianyu Chen ◽  
Dexuan Xiao ◽  
Yanjing Li ◽  
Sirong Shi ◽  
Xiao Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Nucleic Acids ◽  
Osteogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Differentiation Potential ◽  
Tetrahedral Framework

scholarly journals Exendin‑4 promotes osteogenic differentiation of adipose‑derived stem cells and facilitates bone repair

2019 ◽  
Author(s):  
Banglian Deng ◽  
Wenzhong Zhu ◽  
Yansheng Duan ◽  
Yuqian Hu ◽  
Xuefeng Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Bone Repair ◽  
Adipose Derived Stem Cells
Sign in / Sign up

Export Citation Format

Share Document