scholarly journals Feasibility of Ex Vivo Margin Assessment with Hyperspectral Imaging during Breast-Conserving Surgery: From Imaging Tissue Slices to Imaging Lumpectomy Specimen

2021 ◽  
Vol 11 (19) ◽  
pp. 8881
Author(s):  
Esther Kho ◽  
Behdad Dashtbozorg ◽  
Joyce Sanders ◽  
Marie-Jeanne T. F. D. Vrancken Peeters ◽  
Frederieke van Duijnhoven ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Ex Vivo ◽  
Breast Conserving Surgery ◽  
Sample Thickness ◽  
Hyperspectral Images ◽  
Margin Assessment ◽  
Tissue Slices ◽  
High Penetration ◽  
Minimum Sample ◽  
Lumpectomy Specimen

Developing algorithms for analyzing hyperspectral images as an intraoperative tool for margin assessment during breast-conserving surgery requires a dataset with reliable histopathologic labels. The feasibility of using tissue slices hyperspectral dataset with a high correlation with histopathology for developing an algorithm for analyzing the images from the surface of lumpectomy specimens was investigated. We presented a method to acquire hyperspectral images from the lumpectomy surface with a high correlation with histopathology. The tissue slices dataset was compared with the dataset obtained on lumpectomy specimen and the wavelengths with a penetration depth up to the minimum sample thickness of the tissue slices were used to develop a tissue classification algorithm. Spectral differences were observed between tissue slices and lumpectomy datasets due to differences in the sample thickness between both datasets; wavelengths with a high penetration depth were able to penetrate through the thinner tissue slices, affecting the captured signal. By using only wavelengths with a penetration depth up to the minimum sample thickness of the tissue slices, the adipose tissue could be discriminated from other tissue types, but differentiating malignant from connective tissue was more challenging.

scholarly journals Spatially resolved microfluidic stimulation of lymphoid tissue ex vivo

The Analyst ◽  
2017 ◽  
Vol 142 (4) ◽  
pp. 649-659 ◽  
Author(s):  
Ashley E. Ross ◽  
Maura C. Belanger ◽  
Jacob F. Woodroof ◽  
Rebecca R. Pompano
Keyword(s):  
Lymph Node ◽  
Targeted Delivery ◽  
Lymphoid Tissue ◽  
Ex Vivo ◽  
Tissue Slices ◽  
Microfluidic Platform ◽  
Spatially Resolved ◽  
Local Stimulation ◽  
Stimulation Of

We present the first microfluidic platform for local stimulation of lymph node tissue slices and demonstrate targeted delivery of a model therapeutic.

scholarly journals Synthetic Aperture Imaging Using High-Frequency Convex Array for Ophthalmic Ultrasound Applications

Sensors ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 2275
Author(s):  
Hae Gyun Lim ◽  
Hyung Ham Kim ◽  
Changhan Yoon
Keyword(s):  
Image Quality ◽  
Penetration Depth ◽  
Spatial Resolution ◽  
High Frequency ◽  
Imaging System ◽  
Ex Vivo ◽  
Synthetic Aperture ◽  
Single Element ◽  
High Frequency Ultrasound ◽  
Synthetic Aperture Imaging

High-frequency ultrasound (HFUS) imaging has emerged as an essential tool for pre-clinical studies and clinical applications such as ophthalmic and dermatologic imaging. HFUS imaging systems based on array transducers capable of dynamic receive focusing have considerably improved the image quality in terms of spatial resolution and signal-to-noise ratio (SNR) compared to those by the single-element transducer-based one. However, the array system still suffers from low spatial resolution and SNR in out-of-focus regions, resulting in a blurred image and a limited penetration depth. In this paper, we present synthetic aperture imaging with a virtual source (SA-VS) for an ophthalmic application using a high-frequency convex array transducer. The performances of the SA-VS were evaluated with phantom and ex vivo experiments in comparison with the conventional dynamic receive focusing method. Pre-beamformed radio-frequency (RF) data from phantoms and excised bovine eye were acquired using a custom-built 64-channel imaging system. In the phantom experiments, the SA-VS method showed improved lateral resolution (>10%) and sidelobe level (>4.4 dB) compared to those by the conventional method. The SNR was also improved, resulting in an increased penetration depth: 16 mm and 23 mm for the conventional and SA-VS methods, respectively. Ex vivo images with the SA-VS showed improved image quality at the entire depth and visualized structures that were obscured by noise in conventional imaging.

scholarly journals Effect of Irradiation on Tissue Penetration Depth of Doxorubicin after Pressurized Intra-Peritoneal Aerosol Chemotherapy (PIPAC) in a Novel Ex-Vivo Model

2016 ◽  
Vol 7 (8) ◽  
pp. 910-914 ◽  
Author(s):  
Veria Khosrawipour ◽  
Urs Giger-Pabst ◽  
Tanja Khosrawipour ◽  
Yousef Hedayat Pour ◽  
David Diaz-Carballo ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Ex Vivo ◽  
Tissue Penetration ◽  
Ex Vivo Model

scholarly journals An Integration Strategy for Acoustic Metamaterials to Achieve Absorption by Design

2018 ◽  
Vol 8 (8) ◽  
pp. 1247 ◽  
Author(s):  
Min Yang ◽  
Ping Sheng
Keyword(s):  
Absorption Spectrum ◽  
Perforated Plate ◽  
Design Strategy ◽  
Sample Thickness ◽  
Acoustic Metamaterials ◽  
Metamaterial Structure ◽  
Minimum Thickness ◽  
Practical Applications ◽  
Integration Strategy ◽  
Minimum Sample

As much of metamaterials’ properties originate from resonances, the novel characteristics displayed by acoustic metamaterials are a narrow bandwidth and high dispersive in nature. However, for practical applications, broadband is often a necessity. Furthermore, it would even be better if acoustic metamaterials can display tunable bandwidth characteristics, e.g., with an absorption spectrum that is tailored to fit the noise spectrum. In this article we present a designed integration strategy for acoustic metamaterials that not only overcomes the narrow-band Achilles’ heel for acoustic absorption but also achieves such effect with the minimum sample thickness as dictated by the law of nature. The three elements of the design strategy comprise: (a) the causality constraint, (b) the determination of resonant mode density in accordance with the input target impedance, and (c) the accounting of absorption by evanescent waves. Here, the causality constraint relates the absorption spectrum to a minimum sample thickness, derived from the causal nature of the acoustic response. We have successfully implemented the design strategy by realizing three structures of which one acoustic metamaterial structure, comprising 16 Fabry-Perot resonators, is shown to exhibit near-perfect flat absorption spectrum starting at 400 Hz. The sample has a thickness of 10.86 cm, whereas the minimum thickness as dictated by the causality constraint is 10.55 cm in this particular case. A second structure demonstrates the flexible tunability of the design strategy by opening a reflection notch in the absorption spectrum, extending from 600 to 1000 Hz, with a sample thickness that is only 3 mm above the causality minimum. We compare the designed absorption structure with conventional absorption materials/structures, such as the acoustic sponge and micro-perforated plate, with equal thicknesses as the metamaterial structure. In both cases the designed metamaterial structure displays superior absorption performance in the target frequency range.

scholarly journals Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared

2018 ◽  
Vol 115 (37) ◽  
pp. 9080-9085 ◽  
Author(s):  
Jessica A. Carr ◽  
Marianne Aellen ◽  
Daniel Franke ◽  
Peter T. C. So ◽  
Oliver T. Bruns ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Fluorescence Imaging ◽  
Biological Tissue ◽  
Ex Vivo ◽  
Scattered Light ◽  
Image Contrast ◽  
Fluorescence Image ◽  
Tissue Phantoms ◽  
Shortwave Infrared

Recent technology developments have expanded the wavelength window for biological fluorescence imaging into the shortwave infrared. We show here a mechanistic understanding of how drastic changes in fluorescence imaging contrast can arise from slight changes of imaging wavelength in the shortwave infrared. We demonstrate, in 3D tissue phantoms and in vivo in mice, that light absorption by water within biological tissue increases image contrast due to attenuation of background and highly scattered light. Wavelengths of strong tissue absorption have conventionally been avoided in fluorescence imaging to maximize photon penetration depth and photon collection, yet we demonstrate that imaging at the peak absorbance of water (near 1,450 nm) results in the highest image contrast in the shortwave infrared. Furthermore, we show, through microscopy of highly labeled ex vivo biological tissue, that the contrast improvement from water absorption enables resolution of deeper structures, resulting in a higher imaging penetration depth. We then illustrate these findings in a theoretical model. Our results suggest that the wavelength-dependent absorptivity of water is the dominant optical property contributing to image contrast, and is therefore crucial for determining the optimal imaging window in the infrared.

Clinical efficacy of intraoperative ultrasound for margin assessment in breast‐conserving surgery

2020 ◽  
Vol 26 (11) ◽  
pp. 2308-2311
Author(s):  
Jeeyeon Lee ◽  
Ho Yong Park ◽  
Wan Wook Kim ◽  
Chan Sub Park ◽  
Yungeun Ji ◽  
...  
Keyword(s):  
Clinical Efficacy ◽  
Intraoperative Ultrasound ◽  
Breast Conserving Surgery ◽  
Margin Assessment

PROGNOS, ein Programm zur Prognose von Eindringtiefen bei der Applikation von SteinschutzstofTen auf Naturstein / PROGNOS, a program to predict the penetration depth of conserving fluids in natural stone

1996 ◽  
Vol 2 (5) ◽  
pp. 365-374
Author(s):  
J. Schoonbrood
Keyword(s):  
Computer Program ◽  
Penetration Depth ◽  
Natural Stone ◽  
Laboratory Research ◽  
High Quality ◽  
High Penetration ◽  
Predicted Values

Abstract In order to achieve a high quality in the field of conservation of natural stone surfaces it is most important to realize a high penetration depth of the conserving fluids on the besis of extensive laboratory research on the application of conserving fluids the relation between important factors such as quantity absorbed, duration of application, penetration depth, and selected practical relevant conditions has been established. With these results it was possible to develop a computer program which is able to predict penetration depth for a random choise of practical conditions of application. By the option of the comparision of stored real and predicted values of penetration depth destructive tests on the object are quite superfluous.

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