Comparative analysis of local stimulation methods of reparative osteogenesis

Objective To determine the ratio of the main growth factors when using various methods of local stimulation of reparative osteogenesis.Material and methods The study consisted of two parts: in the first part a comparative analysis of the content of growth factors by ELISA was carried out (PDGF – platelet derived growth factor, TGF – transforming growth factor, VEGF – vascular endothelial growth factor, IGF – insulin-like growth factor, BMP6 and BMP7 – morphogenetic proteins 6 and 7), capable of stimulating reparative osteogenesis in blood plasma, plateletrich plasma, red bone marrow and bone autoregenerate. The second part presented the results of approbation of the autoregenerate obtained according to the original method in the framework of an acute experiment on animals.Results The most important cytokines affecting the process of reparative osteogenesis are fibroblast growth factor – FGF1 and bone morphogenetic protein 7 – BMP7. Based on the results of a comparative enzymelinked immunosorbent assay, it has been established that the autoregenerate, obtained by the original method, and a bone marrow aspirate concentrate have the highest osteogenic potential.Conclusion Autoregenerate is an effective and promising means of local stimulation of reparative osteogenesis, and its transplantation is a simple and highly effective procedure.

TIBIAL BONE NONUNION PLASTIC WITH THE USE OF MONOLOCAL OSTEOSYNTHESIS BY RING FIXATORS

Actuality. Monolocal extrafocal osteosynthesis by ring fixators (RF) of tibial nonunion is not a generally accepted method, there are no systematic guidelines for its implementation. These issues need to be further studied. Task. Formulate situational predominant properties of RF; features of application in different condi-tions. To offer a method of nonunion plastics and features of monolocal osteosynthesis of RF and to study its efficiency. Materials and methods. The data of treatment of 16 patients with aseptic nonunion of tibia, who required complex comprehensive specialized medical care with a possible positive result (scores from 51 to 75 according to the evaluation system Non-Union Scoring System - NUSS). Implementation of monolocal extrafocal osteosynthesis of RF nonunion of shin bones after fractures was performed by us taking into account the features that distinguished it from that by fractures. The principal requirement of surgical intervention was the need to treat the center of nonunion, local stimulation of repara-tive processes. The technical features of all components of the intervention in the future were of great im-portance. The so-called blood-saving tactics were used, which were carried out using squeezing and hemostatic tourniquets, tranexamic acid. The results obtained. The results of treatment of victims with nonunion of the tibia with the use of RF in monolocal mode were as follows. Taking in consideration the severity of the lesion, we consider this result to be good. Given the insufficient number of observations, it is necessary to continue the use of the proposed method of treatment and to investigate its effectiveness. Conclusions. 1. The use of monolocal RF in patients with nonunion of tibia after fractures has the advantage of use in cases with episodes of septic inflammation in the past, extensive scarring of soft tissues, short distal fragment and osteoporosis. 2. The peculiarities of monolocal osteosynthesis of RF were the use of thick Ilizarov needles Ø 2.0 mm with their conduction at an angle of two planes. The obtained results of treatment of victims with nonunion of the tibia with the use of RF in the monolocal mode should be considered encouraging.

Prevention and Nursing Research of PICC Catheter-Related Complications in Patients with Digestive System Malignant Tumor Based on Smart Medical Block Chain

Malignant tumors of digestive system mainly include gastric cancer, colorectal cancer, and esophageal cancer, which generally need chemotherapy. PICC refers to peripherally inserted central venous catheter, which plays an important role in the treatment of malignant tumor patients with chemotherapy, and it has the characteristics of high success rate of puncture and reducing the pain of patients. Its principle is to use PICC catheter for drug delivery, which can effectively reduce the pain of tumor patients for multiple puncture, and also can avoid drug extravasation or local stimulation of drugs. However, PICC catheter-related complications cannot be ignored, to some extent, directly affect the treatment effect of patients, and increase the pain and burden of patients. Therefore, this paper proposes a study of PICC catheter-related complications and protective nursing for patients with digestive system cancer based on smart medical block chain. First of all, using the method of literature, this paper deeply studied the combination of smart medicine and block chain and further strengthened the research on PICC catheter-related complications. Based on this, we designed a study on the prevention and nursing of PICC catheter-related complications in patients with digestive system cancer. Before the implementation of nursing, the incidence of complications in patients with digestive system cancer was 17.35%; after the implementation of nursing, the incidence of complications decreased to 4.08%. The purpose of this study is to analyze the causes through clinical research and put forward the protective nursing measures of related diseases, so as to reduce the incidence of PICC-related complications.

Spatial and Temporal Dynamics of Electrical and Photosynthetic Activity and the Content of Phytohormones Induced by Local Stimulation of Pea Plants

A local leaf burning causes variation potential (VP) propagation, a decrease in photosynthesis activity, and changes in the content of phytohormones in unstimulated leaves in pea plants. The VP-induced photosynthesis response develops in two phases: fast inactivation and long-term inactivation. Along with a decrease in photosynthetic activity, there is a transpiration suppression in unstimulated pea leaves, which corresponds to the long-term phase of photosynthesis response. Phytohormone level analysis showed an increase in the concentration of jasmonic acid (JA) preceding a transpiration suppression and a long-term phase of the photosynthesis response. Analysis of the spatial and temporal dynamics of electrical signals, phytohormone levels, photosynthesis, and transpiration activity showed the most pronounced changes in the more distant leaf from the area of local stimulation. The established features are related to the architecture of the vascular bundles in the pea stem.

Localized inhibition in the Drosophila mushroom body

Many neurons show compartmentalized activity, in which activity does not spread readily across the cell, allowing input and output to occur locally. However, the functional implications of compartmentalized activity for the wider neural circuit are often unclear. We addressed this problem in the Drosophila mushroom body, whose principal neurons, Kenyon cells, receive feedback inhibition from a non-spiking interneuron called the anterior paired lateral (APL) neuron. We used local stimulation and volumetric calcium imaging to show that APL inhibits Kenyon cells’ dendrites and axons, and that both activity in APL and APL’s inhibitory effect on Kenyon cells are spatially localized (the latter somewhat less so), allowing APL to differentially inhibit different mushroom body compartments. Applying these results to the Drosophila hemibrain connectome predicts that individual Kenyon cells inhibit themselves via APL more strongly than they inhibit other individual Kenyon cells. These findings reveal how cellular physiology and detailed network anatomy can combine to influence circuit function.

Treatment with Melittin Induces Apoptosis and Autophagy of Fibroblast-like Synoviocytes in Patients with Rheumatoid Arthritis

Background: Melittin, the major medicinal component of honeybee venom, exerts antiinflammatory, analgesic, and anti-arthritic effects in patients with Rheumatoid Arthritis (RA). RA is an inflammatory autoimmune joint disease that leads to irreversible joint destruction and functional loss. Fibroblast-Like Synoviocytes (FLS) are dominant, special mesenchymal cells characterized by the structure of the synovial intima, playing a crucial role in both the initiation and progression of RA. Objective: In this study, we evaluated the effects of melittin on the viability and apoptosis of FLS isolated from patients with RA. Methods: Cell viability was determined using CCK-8 assays; apoptosis was evaluated by flow cytometry, and the expression levels of apoptosis-related proteins (caspase-3, caspase-9, BAX, and Bcl-2) were also determined. To explore whether melittin alters inflammatory processes in RA-FLS, IL-1β levels were determined using an enzyme-linked immunosorbent assay (ELISA). Furthermore, we performed GFP-LC3 punctate fluorescence dot assays and western blotting (for LC3, ATG5, p62, and Beclin 1) to assess autophagy in RA-FLS. Results: Our results show that melittin can significantly impair viability, promote apoptosis and autophagy, and inhibit IL-1β secretion in RA-FLS. Conclusion: Melittin may be useful in preventing damage to the joints during accidental local stimulation.

Localized inhibition in the Drosophila mushroom body

Many neurons show compartmentalized activity, in which activity does not spread readily across the cell, allowing input and output to occur locally. However, the functional implications of compartmentalized activity for the wider neural circuit are often unclear. We addressed this problem in the Drosophila mushroom body, whose principal neurons, Kenyon cells, receive feedback inhibition from a large, non-spiking interneuron called APL. We used local stimulation and volumetric calcium imaging to show that APL inhibits Kenyon cells in both their dendrites and axons, and that both activity in APL and APL’s inhibitory effect on Kenyon cells are spatially localized, allowing APL to differentially inhibit different mushroom body compartments. Applying these results to the Drosophila hemibrain connectome predicts that individual Kenyon cells inhibit themselves via APL more strongly than they inhibit other individual Kenyon cells. These findings reveal how cellular physiology and detailed network anatomy can combine to influence circuit function.

Fleet Transition from Combustion to Electric Vehicles: A Case Study in a Portuguese Business Campus

This paper aims to contribute to the urgent reflection as a society about environmental protection, in the ultimate challenge that is the sustainable use of energy resources. Since Portugal is at an early stage of market development internally, governmental and local stimulation policies play a central role and are a key element in the successful diffusion of Electric Mobility. The study will focus on the transition of a company car fleet, which currently consists of combustion vehicles, to electric vehicles. With this change it becomes necessary to understand how the electrical installation will be affected due to the installation of charging stations, allowing the company to have some autonomy from the public grid. The various changes resulting from the installation consumption profile will be studied and compared. The state of the art, the level of maturity and where the development of electric mobility in Portugal is heading will also be appreciated.