scholarly journals Automated Indirect Transportation of Biological Cells with Optical Tweezers and a 3D Printed Microtool

2019 ◽  
Vol 9 (14) ◽  
pp. 2883 ◽  
Author(s):  
Songyu Hu ◽  
Heng Xie ◽  
Tanyong Wei ◽  
Shuxun Chen ◽  
Dong Sun

Optical tweezers are widely used for noninvasive and precise micromanipulation of living cells to understand biological processes. By focusing laser beams on cells, direct cell manipulation with optical tweezers can achieve high precision and flexibility. However, direct exposure to the laser beam can lead to negative effects on the cells. These phenomena are also known as photobleaching and photodamage. In this study, we proposed a new indirect cell micromanipulation approach combined with a robot-aided holographic optical tweezer system and 3D nano-printed microtool. The microtool was designed with a V-shaped head and an optical handle part. The V-shaped head can push and trap different sizes of cells as the microtool moves forward by optical trapping of the handle part. In this way, cell exposure to the laser beam can be effectively reduced. The microtool was fabricated with a laser direct writing system by two-photon photopolymerization. A control strategy combined with an imaging processing algorithm was introduced for automated manipulation of the microtool and cells. Experiments were performed to verify the effectiveness of our approach. First, automated microtool transportation and rotation were demonstrated with high precision. Second, indirect optical transportations of cells, with and without an obstacle, were performed to demonstrate the effectiveness of the proposed approach. Third, experiments of fluorescent cell manipulation were performed to confirm that, indicated by the photobleaching effect, indirect manipulation with the microtool could induce less laser exposure compared with direct optical manipulation. The proposed method could be useful in complex biomedical applications where precise cell manipulation and less laser exposure are required.

Author(s):  
Sujal Bista ◽  
Sagar Chowdhury ◽  
Satyandra K. Gupta ◽  
Amitabh Varshney

Laser beams can be used to create optical traps that can hold and transport small particles. Optical trapping has been used in a number of applications ranging from prototyping at the microscale to biological cell manipulation. Successfully using optical tweezers requires predicting optical forces on the particle being trapped and transported. Reasonably accurate theory and computational models exist for predicting optical forces on a single particle in the close vicinity of a Gaussian laser beam. However, in practice the workspace includes multiple particles that are manipulated using individual optical traps. It has been experimentally shown that the presence of a particle can cast a shadow on a nearby particle and hence affect the optical forces acting on it. Computing optical forces in the presence of shadows in real-time is not feasible on CPUs. In this paper, we introduce a ray-tracing-based application optimized for GPUs to calculate forces exerted by the laser beams on microparticle ensembles in an optical tweezers system. When evaluating the force exerted by a laser beam on 32 interacting particles, our GPU-based application is able to get a 66-fold speed up compared to a single core CPU implementation of traditional Ashkin’s approach and a 10-fold speedup over its single core CPU-based counterpart.


Author(s):  
Sagar Chowdhury ◽  
Petr Svec ◽  
Chenlu Wang ◽  
Kevin T. Seale ◽  
John P. Wikswo ◽  
...  

Microfluidic devices are well suited for the study of biological objects because of their indirect nature of manipulation and high throughput. However, the cell manipulation process solely depends on the fluid flow and hence precise control is difficult to attain inside a microfluidic chamber. Utilizing optical tweezers as a complementary tool provides precise manipulation control. We have presented an automated cell manipulation approach using optical tweezers operating inside a microfluidic chamber. To test and demonstrate the effectiveness of the approach we have developed a physics-based simulator that is completely automated and allows high precision of manipulation.


Author(s):  
Sujal Bista ◽  
Sagar Chowdhury ◽  
Satyandra K. Gupta ◽  
Amitabh Varshney

Laser beams can be used to create optical traps that can hold and transport small particles. Optical trapping has been used in a number of applications ranging from prototyping at the microscale to biological cell manipulation. Successfully using optical tweezers requires predicting optical forces on the particle being trapped and transported. Reasonably accurate theory and computational models exist for predicting optical forces on a single particle in the close vicinity of a Gaussian laser beam. However, in practice the workspace includes multiple particles that are manipulated using individual optical traps. It has been experimentally shown that the presence of a particle can cast a shadow on a nearby particle and hence affect the optical forces acting on it. Computing optical forces in the presence of shadows in real-time is not feasible on CPUs. In this paper, we introduce a ray-tracing-based application optimized for GPUs to calculate forces exerted by the laser beams on microparticle ensembles in an optical tweezers system. When evaluating the force exerted by a laser beam on 32 interacting particles, our GPU-based approach is able to get a 66-fold speed up compared to a single core CPU implementation of traditional Ashkin's approach and a 10-fold speedup over the single core CPU-based implementation of our approach.


2021 ◽  
Vol 53 (8) ◽  
Author(s):  
Quy Ho Quang ◽  
Thanh Thai Doan ◽  
Kien Bui Xuan ◽  
Thang Nguyen Manh

2008 ◽  
Vol 17 (04) ◽  
pp. 387-394 ◽  
Author(s):  
XIUDONG SUN ◽  
XUECONG LI ◽  
JIANLONG ZHANG

Orientating manipulations of cylindrical particles were performed by optical tweezers. Vertical and horizontal manipulations of Escherichia coli (E. coli) were carried out by changing the trapping depth and the focused laser beam shape. It was found that carbon nanotubes bundles (CNTBs) could be rotated in the linear polarized optical trap until it orientated its long axis along the linear polarization direction of the laser beam. However, E.coli could not be orientated in this way. Corresponding mechanisms were discussed based on the anisomeric electric characters of CNTBs. These orientation technologies of cylindrical objects with optical trap have potential applications in assembling nano-electric devices.


2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jeffrey E. Melzer ◽  
Euan McLeod

AbstractThe fabrication of three-dimensional (3D) microscale structures is critical for many applications, including strong and lightweight material development, medical device fabrication, microrobotics, and photonic applications. While 3D microfabrication has seen progress over the past decades, complex multicomponent integration with small or hierarchical feature sizes is still a challenge. In this study, an optical positioning and linking (OPAL) platform based on optical tweezers is used to precisely fabricate 3D microstructures from two types of micron-scale building blocks linked by biochemical interactions. A computer-controlled interface with rapid on-the-fly automated recalibration routines maintains accuracy even after placing many building blocks. OPAL achieves a 60-nm positional accuracy by optimizing the molecular functionalization and laser power. A two-component structure consisting of 448 1-µm building blocks is assembled, representing the largest number of building blocks used to date in 3D optical tweezer microassembly. Although optical tweezers have previously been used for microfabrication, those results were generally restricted to single-material structures composed of a relatively small number of larger-sized building blocks, with little discussion of critical process parameters. It is anticipated that OPAL will enable the assembly, augmentation, and repair of microstructures composed of specialty micro/nanomaterial building blocks to be used in new photonic, microfluidic, and biomedical devices.


2021 ◽  
Author(s):  
Dongjin Xie ◽  
Qiuyi Luo ◽  
Shen Zhou ◽  
Mei Zu ◽  
Haifeng Cheng

Inkjet printing of functional material has shown a wide range of application in advertzing, OLED display, printed electronics and other specialized utilities that require high-precision, mask-free, direct-writing deposition technique. Nevertheless,...


1995 ◽  
Vol 119 (3-4) ◽  
pp. 403-414 ◽  
Author(s):  
M. Bouchiat ◽  
D. Chauvat ◽  
J. Guéna ◽  
Ph. Jacquier ◽  
M. Lintz ◽  
...  

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