scholarly journals Fecal Recovery of Probiotics Administered as a Multi-Strain Formulation during Antibiotic Treatment

Biomedicines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 83
Author(s):  
Sofia D. Forssten ◽  
Nicolas Yeung ◽  
Arthur C. Ouwehand
Keyword(s):  
Antibiotic Treatment ◽  
Antibiotic Prescription ◽  
Controlled Study ◽  
Bifidobacterium Lactis ◽  
Fecal Samples ◽  
Antibiotic Associated Diarrhea ◽  
Strain Formulation ◽  
Fecal Recovery ◽  
The Subject ◽  
Lactobacillus Acidophilus Ncfm

The present study aimed to investigate whether probiotic recovery is affected when consumed together with antibiotics. Fecal samples were collected from an earlier antibiotic associated diarrhea, randomized, placebo-controlled study with a product consisting of a combination of Lactobacillus acidophilus NCFM, Lactobacillus paracasei Lpc-37, and Bifidobacterium lactis Bi-07, B. lactis Bl-04 at equal numbers and at a total dose of 1010 CFU. Fecal samples were collected during the screening visit (T0), i.e., at the time of antibiotic prescription, and then on the last day of the antibiotic treatment (T1) as well as seven days after the subject had stopped taking the antibiotic treatment (T2) and at two weeks after completing antibiotic treatment and one week after probiotic/placebo consumption stopped (T3). Samples were analyzed for the presence of the four administered strains. The study was registered at clinicaltrials.gov as NCT01596829. Detection levels of all four strains were significantly increased from T0 to T1 and returned to baseline level from T2 to T3. There were also significantly more subjects with detectable levels of L. paracasei Lpc-37, B. lactis Bi-07, and B. lactis Bl-04 at T1 and T2 compared to T0 and T3, and compared to placebo. Each of the four strains could be detected in the feces of patients apparently unaffected by the simultaneous consumption of antibiotics.

scholarly journals Oral iron supplementation after antibiotic exposure induces a deleterious recovery of the gut microbiota

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Thibault Cuisiniere ◽  
Annie Calvé ◽  
Gabriela Fragoso ◽  
Manon Oliero ◽  
Roy Hajjar ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
Iron Supplementation ◽  
Pentose Phosphate ◽  
Oral Iron ◽  
Oral Antibiotic ◽  
Antibiotic Exposure ◽  
Microbiota Composition ◽  
Fecal Samples ◽  
Excess Iron

Abstract Background Oral iron supplementation is commonly prescribed for anemia and may play an important role in the gut microbiota recovery of anemic individuals who received antibiotic treatment. This study aims to investigate the effects of iron supplementation on gut microbiota recovery after antibiotics exposure. Results Mice were subjected to oral antibiotic treatment with neomycin and metronidazole and were fed diets with different concentrations of iron. The composition of the gut microbiota was followed throughout treatment by 16S rRNA sequencing of DNA extracted from fecal samples. Gut microbiota functions were inferred using PICRUSt2, and short-chain fatty acid concentration in fecal samples was assessed by liquid-chromatography mass spectrometry. Iron supplementation after antibiotic exposure shifted the gut microbiota composition towards a Bacteroidetes phylum-dominant composition. At the genus level, the iron-supplemented diet induced an increase in the abundance of Parasutterella and Bacteroides, and a decrease of Bilophila and Akkermansia. Parasutterella excrementihominis, Bacteroides vulgatus, and Alistipes finegoldii, were more abundant with the iron excess diet. Iron-induced shifts in microbiota composition were accompanied by functional modifications, including an enhancement of the biosynthesis of primary bile acids, nitrogen metabolism, cyanoamino acid metabolism and pentose phosphate pathways. Recovery after antibiotic treatment increased propionate levels independent of luminal iron levels, whereas butyrate levels were diminished by excess iron. Conclusions Oral iron supplementation after antibiotic therapy in mice may lead to deleterious changes in the recovery of the gut microbiota. Our results have implications on the use of oral iron supplementation after antibiotic exposure and justify further studies on alternative treatments for anemia in these settings.

scholarly journals Parasitological survey on birds at some selected brazilian zoos

2015 ◽  
Vol 24 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Paulo Gonzalez Hofstatter ◽  
Ana Maria Aparecida Guaraldo
Keyword(s):  
Bird Species ◽  
Sao Paulo ◽  
Parasite Fauna ◽  
São Paulo ◽  
Fecal Samples ◽  
Parasitological Survey ◽  
The Subject ◽  
Coccidian Species ◽  
Free Ranging

A parasitological survey was conducted at some zoos in the states of São Paulo and Paraná, Brazil, from 2009 to 2011. Several groups of birds were surveyed for fecal samples, but the most important was Psittacidae. Among the parasites, Eimeria (coccidian) and Capillaria, Ascaridia and Heterakis (nematodes) were observed in almost one third of the samples. Presence of a rich parasite fauna associated with captive birds seems to be an effect of captivity, since data on free-ranging birds indicate few or virtually no parasites at all. The discovery of new coccidian species during this survey reveals the need of more research on the subject as even well-known bird species have unknown parasites, but caution must be exercised in order to avoid descriptions of pseudoparasites.

Long-Term Antibiotic Treatment of Children with Secretory Otitis Media: A Double-Blind Placebo-Controlled Study

1988 ◽  
Vol 105 (sup449) ◽  
pp. 49-50 ◽  
Author(s):  
J. Thomsen ◽  
J. Sederberg-Olsen ◽  
S. E. Stangerup ◽  
V. Balle ◽  
R. Vejlsgaard
Keyword(s):  
Otitis Media ◽  
Antibiotic Treatment ◽  
Controlled Study ◽  
Secretory Otitis Media ◽  
Double Blind ◽  
Double Blind Placebo

Antibiotic Treatment of Acute Otorrhea Through Tympanostomy Tube: Randomized Double-Blind Placebo-Controlled Study With Daily Follow-up

PEDIATRICS ◽  
2003 ◽  
Vol 111 (5) ◽  
pp. 1061-1067 ◽  
Author(s):  
A. Ruohola ◽  
T. Heikkinen ◽  
O. Meurman ◽  
T. Puhakka ◽  
N. Lindblad ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Controlled Study ◽  
Tympanostomy Tube ◽  
Double Blind ◽  
Double Blind Placebo

Effect of Compression Stockings on the Skin Microcirculation in Chronic Venous Insufficiency

1995 ◽  
Vol 10 (1) ◽  
pp. 5-11 ◽  
Author(s):  
A. Abu-Own ◽  
J. H. Scurr ◽  
P. D. Coleridge Smith
Keyword(s):  
Chronic Venous Insufficiency ◽  
Venous Insufficiency ◽  
Laser Doppler ◽  
Controlled Study ◽  
Fibre Laser ◽  
Medial Malleolus ◽  
Compression Stocking ◽  
Compression Stockings ◽  
Median Increase ◽  
The Subject

Objective: To use a single fibre laser Doppler fluxmeter to assess the microcirculatory effects of compression stockings. Design: Controlled study comprising patient and control groups. Setting: Department of Surgery, University College London Medical School, London, UK. Patients and participants: Ten patients with lipodermatosclerosis caused by chronic venous insufficiency and 10 control subjects. Interventions: Measurements were made from the liposclerotic skin of patients and 8 cm above the medial malleolus in controls. Laser Doppler flux (LDF), blood ce velocity (BCV) and concentration of moving blood cells (CMBC) were recorded with the subject lying supine. A class II graduated compression stocking was applied to the leg and laser Doppler recordings were repeated. The protocol was repeated with the subject sitting. Main outcome measures: The effects of a compression stocking on LDF, BCV and CMBC in the horizontal and sitting positions were measured. Results: In patients in the supine position, the compression stocking resulted in a 28% median increase in LDF ( p = 0.03), with a corresponding 29% median increase in BCV. There was no significant change in CMBC. In the sitting position, the compression stocking caused a 105% median increase in LDF ( p < 0.01) due to a corresponding 89% median increase in BCV ( p = 0.01); there was only 25% median increase in CMBC. The effects of compression in controls were similar to those in patients. Conclusion: Compression stockings may be effective in the treatment of chronic venous insufficiency by increasing the microcirculatory flow velocity.

scholarly journals Saccharomyces boulardii to Prevent Antibiotic-Associated Diarrhea: A Randomized, Double-Masked, Placebo-Controlled Trial

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Stephan Ehrhardt ◽  
Nan Guo ◽  
Rebecca Hinz ◽  
Stefanie Schoppen ◽  
Jürgen May ◽  
...  
Keyword(s):  
Placebo Group ◽  
Clostridium Difficile ◽  
Adverse Events ◽  
Antibiotic Treatment ◽  
Controlled Trial ◽  
Saccharomyces Boulardii ◽  
Serious Adverse Events ◽  
Antibiotic Associated Diarrhea ◽  
Clostridium Difficile Associated Diarrhea ◽  
Systemic Antibiotic Treatment

Abstract Background.  Antibiotic-associated diarrhea (AAD) and Clostridium difficile-associated diarrhea (CDAD) are common complications of antibiotic use. Data on the efficacy of probiotics to prevent AAD and CDAD are unclear. We aimed to evaluate the efficacy of Saccharomyces boulardii to prevent AAD and CDAD in hospitalized adult patients. Methods.  We conducted a multicenter, phase III, double-masked, randomized, placebo-controlled trial in hospitalized patients who received systemic antibiotic treatment in 15 hospitals in Germany between July 2010 and October 2012. Participants received Perenterol forte 250 mg capsules or matching placebo twice per day within 24 hours of initiating antibiotic treatment, continued treatment for 7 days after antibiotic discontinuation, and were then observed for 6 weeks. Results.  Two thousand four hundred forty-four patients were screened. The trial was stopped early for futility after inclusion of 477 participants. Two hundred forty-six patients aged 60.1 ± 16.5 years and 231 patients aged 56.5 ± 17.8 were randomized to the S boulardii group and the placebo group, respectively, with 21 and 19 AADs in the respective groups (P = .87). The hazard ratio of AAD in the S boulardii group compared with the placebo group was 1.02 (95% confidence interval, .55–1.90; P = .94). Clostridium difficile-associated diarrhea occurred in 0.8% of participants (4 of 477). Nine serious adverse events were recorded in the S boulardii group, and 3 serious adverse events were recorded in the placebo group. None were related to study participation. Conclusions.  We found no evidence for an effect of S boulardii in preventing AAD or CDAD in a population of hospitalized patients without particular risk factors apart from systemic antibiotic treatment. ClinicalTrials.gov Identifier.  NCT01143272.

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