scholarly journals Differences in Distribution and Biological Effects of F3O4@PEG Nanoparticles in Normotensive and Hypertensive Rats—Focus on Vascular Function and Liver

Biomedicines ◽  
2021 ◽  
Vol 9 (12) ◽  
pp. 1855
Author(s):  
Andrea Micurova ◽  
Michal Kluknavsky ◽  
Silvia Liskova ◽  
Peter Balis ◽  
Martin Skratek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vascular Function ◽  
Biological Effects ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Magnetite Fe3o4 ◽  
Peg Treatment ◽  
Wistar Kyoto ◽  
Oxide Synthase ◽  
Main Effects

We investigate the distribution and biological effects of polyethylene glycol (PEG)-coated magnetite (Fe3O4@PEG) nanoparticles (~30 nm core size, ~51 nm hydrodynamic size, 2 mg Fe/kg/day, intravenously, for two days) in the aorta and liver of Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR). Fe3O4@PEG had no effect on open-field behaviour but reduced the blood pressure (BP) of Fe3O4@PEG-treated SHR (SHRu) significantly, compared to both Fe3O4@PEG-treated WKY (WKYu) and saline-treated control SHR (SHRc). The Fe3O4@PEG content was significantly elevated in the aorta and liver of SHRu vs. WKYu. Nitric oxide synthase (NOS) activity was unaltered in the aorta, but significantly increased in the liver of SHRu vs. SHRc. In the aorta, Fe3O4@PEG treatment increased eNOS, iNOS, NRF2, and DMT1 gene expression (considered main effects). In the liver, Fe3O4@PEG significantly elevated eNOS and iNOS gene expression in SHRu vs. SHRc, as well as DMT1 and FTH1 gene expression (considered main effects). Noradrenaline-induced contractions of the femoral arteries were elevated, while endothelium-dependent contractions were reduced in SHRu vs. SHRc. No differences were found in these parameters in WKY rats. In conclusion, the results indicated that the altered haemodynamics in SHR affect the tissue distribution and selected biological effects of Fe3O4@PEG in the vasculature and liver, suggesting that caution should be taken when using iron oxide nanoparticles in hypertensive subjects.

scholarly journals Effect of Deuterium-Depleted Water on Selected Cardiometabolic Parameters in Fructose-Treated Rats

2016 ◽  
pp. S401-S407 ◽  
Author(s):  
R. REHAKOVA ◽  
J. KLIMENTOVA ◽  
M. CEBOVA ◽  
A. BARTA ◽  
Z. MATUSKOVA ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Insulin Level ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Protein Expressions ◽  
Deuterium Depleted Water ◽  
Wistar Kyoto ◽  
Oxide Synthase ◽  
Enos Protein

Deuterium-depleted water (DDW) has a lower concentration of deuterium than occurs naturally (less than 145 ppm). While effects of DDW on cancer started to be intensively studied, the effects on cardiovascular system are completely unknown. Thus, we aimed to analyze the effects of DDW (55±5 ppm) administration to 12-week-old normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) treated with 15 % fructose for 6 weeks. Blood pressure (BP) and selected biochemical parameters were measured together with determination of nitric oxide synthase (NOS) activity and iNOS and eNOS protein expressions in the left ventricle (LV) and aorta. Neither DDW nor fructose had any significant effect on BP in both strains. DDW treatment decreased total cholesterol and triglyceride levels in WKY, but it was not able to prevent increase in the same parameters elevated due to fructose treatment in SHR. Both fructose and DDW increased insulin level in WKY. Fructose did not affect NOS activity either in WKY or SHR. DDW increased NOS activity in LV of both WKY and SHR, while it decreased NOS activity and iNOS expression in the aorta of SHR with or without fructose treatment. In conclusion, DDW treatment significantly modified biochemical parameters in WKY together with NOS activity elevation in the heart. On the other hand, it did not affect biochemical parameters in SHR, but decreased NOS activity elevated due to iNOS upregulation in the aorta.

Farrerol Modulates Aorta Gene Expression Profile in Spontaneously Hypertensive Rats

Planta Medica ◽  
2017 ◽  
Vol 84 (05) ◽  
pp. 296-303 ◽  
Author(s):  
Xiaojiang Qin ◽  
Xiaomin Hou ◽  
Kun Zhang ◽  
Qingshan Li
Keyword(s):  
Gene Expression ◽  
Smooth Muscle ◽  
Muscle Contraction ◽  
Signaling Pathway ◽  
Spontaneously Hypertensive Rats ◽  
Smooth Muscle Contraction ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Vascular Smooth Muscle Contraction ◽  
Wistar Kyoto

AbstractFarrerol, a typical natural flavanone and major active component in Rhododendron dauricum var. ciliatum, has been shown to possess vasoactive ability in vitro. The aim of this study was to investigate its effect on aorta gene expression in spontaneously hypertensive rats. Twelve-week-old male normotensive Wistar Kyoto rats and spontaneously hypertensive rats were treated with orally administered farrerol (50 mg/kg body weight) for 8 wk before they were sacrificed. We found that aorta samples showed 444 upregulated genes in control spontaneously hypertensive rats compared with the control Wistar Kyoto rats. Administration of farrerol in spontaneously hypertensive rats increased the expression of 2329 genes in the aorta compared with the control spontaneously hypertensive rats. Gene expression profiles performed on the aorta revealed that farrerol induced changes in vascular smooth muscle contraction, mitogen-activated protein kinase signaling pathway, regulation of actin cytoskeleton, vascular endothelial growth factor signaling pathway, calcium signaling pathway, and renin angiotensin system. Furthermore, 10 genes involved in the pathway of vascular smooth muscle contraction were verified using real-time polymerase chain reaction technique, and several novel potential target genes for the farrerol treatment of hypertension were identified. The findings of this study lend support to the potential use of farrerol as a novel therapeutic and antihypertensive candidate drug to prevent the development of hypertension.

scholarly journals Distinct Gene Expression Profiles in Colonic Organoids from Normotensive and the Spontaneously Hypertensive Rats

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1523
Author(s):  
Jing Li ◽  
Elaine M. Richards ◽  
Eileen M. Handberg ◽  
Carl J. Pepine ◽  
Mohan K. Raizada
Keyword(s):  
Gene Expression ◽  
Immune Responses ◽  
Gut Microbiome ◽  
Spontaneously Hypertensive Rats ◽  
Expression Profiles ◽  
Hypertensive Rats ◽  
Gene Expressions ◽  
Spontaneously Hypertensive ◽  
Gut Epithelium ◽  
Wistar Kyoto

Hypertension is associated with gut bacterial dysbiosis and gut pathology in animal models and people. Butyrate-producing gut bacteria are decreased in hypertension. RNA-seq analysis of gut colonic organoids prepared from spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats was used to test the hypothesis that impaired interactions between the gut microbiome and gut epithelium are involved and that these would be remediated with butyrate supplementation. Gene expressions in immune responses including antigen presentation and antiviral pathways were decreased in the gut epithelium of the SHR in organoids and confirmed in vivo; these deficits were corrected by butyrate supplementation. Deficits in gene expression driving epithelial proliferation and differentiation were also observed in SHR. These findings highlight the importance of aligned interactions of the gut microbiome and gut immune responses to blood pressure homeostasis.

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