Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence

Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of Synechococcus. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring.

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Polarized light scattering of nucleosomes and polynucleosomes-in situ and in vitro studies

IEEE Transactions on Biomedical Engineering ◽

10.1109/10.83568 ◽

1991 ◽

Vol 38 (7) ◽

pp. 670-678 ◽

Cited By ~ 18

Author(s):

A. Diaspro ◽

M. Bertolotto ◽

L. Vergani ◽

C. Nicolini

Keyword(s):

Light Scattering ◽

Polarized Light ◽

In Vitro Studies

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Differentiation of suspended particles by polarized light scattering at 120°

Optics Express ◽

10.1364/oe.26.022419 ◽

2018 ◽

Vol 26 (17) ◽

pp. 22419 ◽

Cited By ~ 12

Author(s):

Yong Wang ◽

Ran Liao ◽

Jincai Dai ◽

Zhidi Liu ◽

Zhihang Xiong ◽

...

Keyword(s):

Light Scattering ◽

Polarized Light ◽

Suspended Particles

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In-situ Detection Method for Microplastics in Water by Polarized Light Scattering

Frontiers in Marine Science ◽

10.3389/fmars.2021.739683 ◽

2021 ◽

Vol 8 ◽

Author(s):

Tong Liu ◽

Shijun Yu ◽

Xiaoshan Zhu ◽

Ran Liao ◽

Zepeng Zhuo ◽

...

Keyword(s):

Light Scattering ◽

Scattered Light ◽

Polarized Light ◽

Chemical Properties ◽

Future Application ◽

Physical And Chemical Properties ◽

The Individual ◽

Physical And Chemical ◽

And Chemical Properties

Microplastics (MPs) have become the widespread contaminants, which raises concerns on their ecological hazards. In-situ detection of MP in water bodies is essential for clear assessment of the ecological risks of MPs. The present study proposes a method based on polarized light scattering which measures the polarization parameters of the scattered light at 120° to detect MP in water. This method takes the advantage of in-situ measurement of the individual particles and the experimental setup in principle is used. By use of the measured polarization parameters equipped by machine learning, the standard polystyrene (PS) spheres, natural water sample, and lab-cultured microalgae are explicitly discriminated, and MP with different physical and chemical properties can be differentiated. It can also characterize the weathering of different MP and identify the specific type from multiple types of MP. This study explores the capability of the proposed method to detect the physical and chemical properties, weathering state and concentration of MP in water which promises the future application in water quality sensing and monitoring.

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Modeling of polarized light scattering in cirrus clouds: validation with in-situ measurements and ADEOS-POLDER reflectance observations

10.1117/12.366341 ◽

1999 ◽

Author(s):

Laurent C. Labonnote ◽

Gerard Brogniez ◽

Jean-Francois Gayet ◽

Jean-Claude Buriez ◽

Marie Doutriaux-Boucher

Keyword(s):

Light Scattering ◽

Polarized Light ◽

In Situ Measurements ◽

Cirrus Clouds

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Demineralization and Remineralization Evaluation Techniques

Journal of Dental Research ◽

10.1177/002203459207100s27 ◽

1992 ◽

Vol 71 (3_suppl) ◽

pp. 924-928 ◽

Cited By ~ 152

Author(s):

J. Arends ◽

J.J. ten Bosch

Keyword(s):

Light Scattering ◽

Mineral Content ◽

Polarized Light ◽

Cross Sectional ◽

Main Emphasis ◽

Measuring Techniques ◽

Mineral Loss ◽

Mineral Changes

This paper compares the experimental techniques utilized to assess the de- or remineralization of enamel or dentin in intra-oral studies. In in situ studies, it is important for one to know how much mineral has been lost or gained, and where the loss or gain occurred. The main emphasis in this paper is on techniques suitable for direct or indirect mineral quantification. The measuring techniques considered are microradiography, iodine absorptiometry, various microhardness tests, polarized light, light-scattering, iodide permeability, and wet chemical analysis. The various techniques are compared concerning suitability for the determination of mineral content in vol% (or wt%), mineral changes in vol% μm (or kg.m–2), and mineral distributions. Furthermore, sample preparation, the importance of protein penetration, nominal mineral loss range, the estimated mineral loss threshold, and the applicability to dentin are compared and considered. It is concluded that, although more than ten techniques are available for the measurement of changes after de- or remineralization in situ, transverse microradiography is the most practical technique for the direct and quantitative measurement of mineral content, mineral changes, and mineral distributions. Cross-sectional microhardness testing and light-scattering are also practical indirect techniques for quantitative mineral loss (or gain) determinations in intra-oral studies.

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Optimized Classification of Suspended Particles in Seawater by Dense Sampling of Polarized Light Pulses

Sensors ◽

10.3390/s21217344 ◽

2021 ◽

Vol 21 (21) ◽

pp. 7344

Author(s):

Zhiming Guo ◽

Hanbo Deng ◽

Jiajin Li ◽

Ran Liao ◽

Hui Ma

Keyword(s):

Sampling Method ◽

Red Tide ◽

Marine Ecosystem ◽

Polarized Light ◽

Suspended Particles ◽

Classification Performance ◽

Individual Measurement ◽

Light Pulses ◽

Dense Sampling

Suspended particles affect the state and vitality of the marine ecosystem. In situ probing and accurately classifying the suspended particles in seawater have an important impact on ecological research and environmental monitoring. Individual measurement of the optical polarization parameters scattered by the suspended particles has been proven to be a powerful tool to classify the particulate compositions in seawater. In previous works, the temporal polarized light pulses are sampled and averaged to evaluate the polarization parameters. In this paper, a method based on dense sampling of polarized light pulses is proposed and the experimental setup is built. The experimental results show that the dense sampling method optimizes the classification and increases the average accuracy by at least 16% than the average method. We demonstrate the feasibility of dense sampling method by classifying the multiple types of particles in mixed suspensions and show its excellent generalization ability by multi-classification of the particles. Additional analysis indicates that the dense sampling method basically takes advantage of the high-quality polarization parameters to optimize the classification performance. The above results suggest that the proposed dense sampling method has the potential to probe the suspended particles in seawater in red-tide early warning, as well as sediment and microplastics monitoring.

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In-Situ Characterization of Nanoparticles by Polarized Light Scattering and Laser-Induced Incandescence

KONA Powder and Particle Journal ◽

10.14356/kona.1998023 ◽

1998 ◽

Vol 16 (0) ◽

pp. 207-215 ◽

Cited By ~ 3

Author(s):

Reiner Weichert

Keyword(s):

Light Scattering ◽

Polarized Light ◽

In Situ Characterization ◽

Laser Induced Incandescence

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Probing the Cyanobacterial Microcystis Gas Vesicles after Static Pressure Treatment: A Potential In Situ Rapid Method

Sensors ◽

10.3390/s20154170 ◽

2020 ◽

Vol 20 (15) ◽

pp. 4170

Author(s):

Jiajin Li ◽

Ran Liao ◽

Yi Tao ◽

Zepeng Zhuo ◽

Zhidi Liu ◽

...

Keyword(s):

Light Scattering ◽

Vertical Migration ◽

Static Pressure ◽

Polarized Light ◽

Cyanobacterial Blooms ◽

Scattering Method ◽

Pressure Treatment ◽

Light Conditions ◽

Gas Vesicles

The vertical migration trend of cyanobacterial cells with gas vesicles in water ecosystems can reflect the changes in the natural environment, such as temperature, nutrients, light conditions, etc. The static pressure treatment is one of the most important approaches to study the properties of the cyanobacterial cell and its gas vesicles. In this paper, a polarized light scattering method is used to probe the collapse and regeneration of the cyanobacterial gas vesicles exposed to different static pressures. During the course, both the axenic and wild type strain of cyanobacterial Microcystis were first treated with different static pressures and then recovered on the normal light conditions. Combining the observation of transmission electron microscopy and floating-sinking photos, the results showed that the collapse and regeneration of the cyanobacterial gas vesicles exposed to different static pressures can be characterized by the polarization parameters. The turbidity as a traditional indicator of gas vesicles but subjected to the concentration of the sample was also measured and found to be correlated with the polarization parameters. More analysis indicated that the polarization parameters are more sensitive and characteristic. The polarized light scattering method can be used to probe the cyanobacterial gas vesicles exposed to different static pressures, which has the potential to provide an in situ rapid and damage-free monitoring tool for observing the vertical migration of cyanobacterial cells and forecasting cyanobacterial blooms.

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Measurements of the Volume Scattering Function and the Degree of Linear Polarization of Light Scattered by Contrasting Natural Assemblages of Marine Particles

Applied Sciences ◽

10.3390/app8122690 ◽

2018 ◽

Vol 8 (12) ◽

pp. 2690 ◽

Cited By ~ 8

Author(s):

Daniel Koestner ◽

Dariusz Stramski ◽

Rick Reynolds

Keyword(s):

Light Scattering ◽

Linear Polarization ◽

Suspended Particles ◽

Mie Scattering ◽

Natural Seawater ◽

Laboratory Measurements ◽

Scattering Function ◽

Volume Scattering ◽

Organic Particles

The light scattering properties of seawater play important roles in radiative transfer in the ocean and optically-based methods for characterizing marine suspended particles from in situ and remote sensing measurements. The recently commercialized LISST-VSF instrument is capable of providing in situ or laboratory measurements of the volume scattering function, β p ( ψ ) , and the degree of linear polarization, DoLP p ( ψ ) , associated with particle scattering. These optical quantities of natural particle assemblages have not been measured routinely in past studies. To fully realize the potential of LISST-VSF measurements, we evaluated instrument performance, and developed calibration correction functions from laboratory measurements and Mie scattering calculations for standard polystyrene beads suspended in water. The correction functions were validated with independent measurements. The improved LISST-VSF protocol was applied to measurements of β p ( ψ ) and DoLP p ( ψ ) taken on 17 natural seawater samples from coastal and offshore marine environments characterized by contrasting assemblages of suspended particles. Both β p ( ψ ) and DoLP p ( ψ ) exhibited significant variations related to a broad range of composition and size distribution of particulate assemblages. For example, negative relational trends were observed between the particulate backscattering ratio derived from β p ( ψ ) and increasing proportions of organic particles or phytoplankton in the particulate assemblage. Our results also suggest a potential trend between the maximum values of DoLP p ( ψ ) and particle size metrics, such that a decrease in the maximum DoLP p ( ψ ) tends to be associated with particulate assemblages exhibiting a higher proportion of large-sized particles. Such results have the potential to advance optically-based applications that rely on an understanding of relationships between light scattering and particle properties of natural particulate assemblages.

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