scholarly journals Detection of Hypertension-Induced Changes in Erythrocytes by SERS Nanosensors

Biosensors ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 32
Author(s):  
Evelina I. Nikelshparg ◽  
Adil A. Baizhumanov ◽  
Zhanna V. Bochkova ◽  
Sergey M. Novikov ◽  
Dmitry I. Yakubovsky ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cellular Level ◽  
Molecular Changes ◽  
Plasma Membrane Fluidity ◽  
Promising Tool ◽  
Surface Enhanced Raman ◽  
Membrane Bound ◽  
Erythrocyte Plasma Membrane ◽  
Induced Changes

Surface-enhanced Raman spectroscopy (SERS) is a promising tool that can be used in the detection of molecular changes triggered by disease development. Cardiovascular diseases (CVDs) are caused by multiple pathologies originating at the cellular level. The identification of these deteriorations can provide a better understanding of CVD mechanisms, and the monitoring of the identified molecular changes can be employed in the development of novel biosensor tools for early diagnostics. We applied plasmonic SERS nanosensors to assess changes in the properties of erythrocytes under normotensive and hypertensive conditions in the animal model. We found that spontaneous hypertension in rats leads (i) to a decrease in the erythrocyte plasma membrane fluidity and (ii) to a decrease in the mobility of the heme of the membrane-bound hemoglobin. We identified SERS parameters that can be used to detect pathological changes in the plasma membrane and submembrane region of erythrocytes.

Erythrocyte plasma membrane fluidity in avian muscular dystrophy

1979 ◽  
Vol 64 (2) ◽  
pp. 315-326 ◽  
Author(s):  
Thomas B. Eckstein ◽  
William R. Randall ◽  
Mark G. McNamee
Keyword(s):  
Plasma Membrane ◽  
Muscular Dystrophy ◽  
Membrane Fluidity ◽  
Plasma Membrane Fluidity ◽  
Erythrocyte Plasma Membrane

scholarly journals The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Pin Gao ◽  
Bing Han ◽  
Ye Du ◽  
Gang Zhao ◽  
Zhigang Yu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Raman Spectroscopy ◽  
Cancer Diagnosis ◽  
Breast Cancer Diagnosis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cellular Level ◽  
Clinical Tool ◽  
Normal Tissues ◽  
Surface Enhanced Raman

Raman spectroscopy has been widely used as an important clinical tool for real-time in vivo cancer diagnosis. Raman information can be obtained from whole organisms and tissues, at the cellular level and at the biomolecular level. The aim of this paper is to review the newest developments of Raman spectroscopy in the field of breast cancer diagnosis and treatment. Raman spectroscopy can distinguish malignant tissues from noncancerous/normal tissues and can assess tumor margins or sentinel lymph nodes during an operation. At the cellular level, Raman spectra can be used to monitor the intracellular processes occurring in blood circulation. At the biomolecular level, surface-enhanced Raman spectroscopy techniques may help detect the biomarker on the tumor surface as well as evaluate the efficacy of anticancer drugs. Furthermore, Raman images reveal an inhomogeneous distribution of different compounds, especially proteins, lipids, microcalcifications, and their metabolic products, in cancerous breast tissues. Information about these compounds may further our understanding of the mechanisms of breast cancer.

scholarly journals Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue

2019 ◽  
Vol 20 (24) ◽  
pp. 6306
Author(s):  
Aleksandra M. Bondžić ◽  
Andreja R. Leskovac ◽  
Sandra Ž. Petrović ◽  
Dragana D. Vasić Anićijević ◽  
Marco Luce ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Raman Spectroscopy ◽  
Density Functional ◽  
Human Lymphocytes ◽  
Physicochemical Characterization ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced Raman ◽  
Sers Detection ◽  
Cell Fractions ◽  
Induced Changes

Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.

scholarly journals The Study of Naphthoquinones and Their Complexes with DNA by Using Raman Spectroscopy and Surface Enhanced Raman Spectroscopy: New Insight into Interactions of DNA with Plant Secondary Metabolites

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Veronika Vaverkova ◽  
Oldrich Vrana ◽  
Vojtech Adam ◽  
Tomas Pekarek ◽  
Josef Jampilek ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Secondary Metabolites ◽  
Plant Secondary Metabolites ◽  
Analytical Techniques ◽  
Surface Enhanced Raman Spectroscopy ◽  
Cellular Level ◽  
Cytotoxic Action ◽  
Structure Sensitive ◽  
Surface Enhanced Raman ◽  
Cytotoxic Compounds

Naphthoquinones represent the group of plant secondary metabolites with cytotoxic properties based on their ability to generate reactive oxygen species and interfere with the processes of cell respiration. Due to this fact, the possible cytotoxic mechanisms on cellular and subcellular levels are investigated intensively. There are many targets of cytotoxic action on the cellular level; however, DNA is a critical target of many cytotoxic compounds. Due to the cytotoxic properties of naphthoquinones, it is necessary to study the processes of naphthoquinones, DNA interactions (1,4-naphthoquinone, binapthoquinone, juglone, lawsone, plumbagin), especially by using modern analytical techniques. In our work, the Raman spectroscopy was used to determine the possible binding sites of the naphthoquinones on the DNA and to characterize the bond of naphthoquinone to DNA. Experimental data reveals the relationships between the perturbations of structure-sensitive Raman bands and the types of the naphthoquinones involved. The modification of DNA by the studied naphthoquinones leads to the nonspecific interaction, which causes the transition of B-DNA into A-DNA conformation. The change of the B-conformation of DNA for all measured DNA modified by naphthoquinones except plumbagin is obvious.

scholarly journals A study of the Interaction Between Cetirizine and Plasma Membrane of Eosinophils, Neutrophils, Platelets and Lymphocytes using A fluorescence Technique

1994 ◽  
Vol 3 (3) ◽  
pp. 229-234 ◽  
Author(s):  
A. Kantar ◽  
N. Oggiano ◽  
P. L. Giorgi ◽  
J-P. Rihoux
Keyword(s):  
Plasma Membrane ◽  
Membrane Fluidity ◽  
Fluorescence Anisotropy ◽  
Inflammatory Cell ◽  
Fluorescence Decay ◽  
Fluorescence Technique ◽  
Membrane Heterogeneity ◽  
Plasma Membrane Fluidity ◽  
Steady State Fluorescence ◽  
Induced Changes

The effect of cetirizine on plasma membrane fluidity and heterogeneity of human eosinophils, neutrophils, platelets and lymphocytes was investigated using a fluorescence technique. Membrane fluidity and heterogeneity were studied by measuring the steady-state fluorescence anisotropy and fluorescence decay of 1-(4- trimethylammonium-phenyl)-6-phenyl-1, 3, 5-hexatriene (TMA-DPH) incorporated in the membrane. The results demonstrate that cetirizine (1 μg/ml) induced a significant increase in the Hpid order in the exterior part of the membrane and a decrease in membrane heterogeneity in eosinophils, neutrophils and platelets. Moreover, cetirizine blocked the PAF induced changes in membrane fluidity in these cells. Cetirizine did not influence significantly the plasma membrane of lymphocytes. These data may partially explain the effect ofcetirizine on inflammatory cell activities.

scholarly journals A Comparative Study of Approaches to Improve the Sensitivity of Lateral Flow Immunoassay of the Antibiotic Lincomycin

Biosensors ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 198
Author(s):  
Kseniya V. Serebrennikova ◽  
Olga D. Hendrickson ◽  
Elena A. Zvereva ◽  
Demid S. Popravko ◽  
Anatoly V. Zherdev ◽  
...  
Keyword(s):  
Point Of Care ◽  
Fluorescence Analysis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Quantitative Detection ◽  
Assay Sensitivity ◽  
Detection Techniques ◽  
Promising Tool ◽  
Surface Enhanced Raman

This study provides a comparative assessment of the various nanodispersed markers and related detection techniques used in the immunochromatographic detection of an antibiotic lincomycin (LIN). Improving the sensitivity of the competitive lateral flow immunoassay is important, given the increasing demands for the monitoring of chemical contaminants in food. Gold nanoparticles (AuNPs) and CdSe/ZnS quantum dots (QDs) were used for the development and comparison of three approaches for the lateral flow immunoassay (LFIA) of LIN, namely, colorimetric, fluorescence, and surface-enhanced Raman spectroscopy (SERS)-based LFIAs. It was demonstrated that, for colorimetric and fluorescence analysis, the detection limits were comparable at 0.4 and 0.2 ng/mL, respectively. A SERS-based method allowed achieving the gain of five orders of magnitude in the assay sensitivity (1.4 fg/mL) compared to conventional LFIAs. Therefore, an integration of a SERS reporter into the LFIA is a promising tool for extremely sensitive quantitative detection of target analytes. However, implementation of this time-consuming technique requires expensive equipment and skilled personnel. In contrast, conventional AuNP- and QD-based LFIAs can provide simple, rapid, and inexpensive point-of-care testing for practical use.

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