scholarly journals Proton Magnetic Resonance Spectroscopy Characterization of Rathke’s Cleft Cysts (RCCs): Relevance to the Differential Diagnosis of Pituitary Adenomas and RCCs

Cancers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 360
Author(s):  
Omkar B. Ijare ◽  
Martyn A. Sharpe ◽  
David S. Baskin ◽  
Kumar Pichumani
Keyword(s):  
Differential Diagnosis ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Pituitary Adenomas ◽  
Ex Vivo ◽  
Medical Professionals ◽  
Resonance Spectroscopy ◽  
Rathke's Cleft Cysts ◽  
Rathke's Cleft

Background: Rathke’s Cleft Cysts (RCCs) are rare epithelial cysts arising from remnants of the Rathke pouch in the pituitary gland. A subset of these lesions enlarge and produce a mass effect with consequent hypopituitarism, and may result in visual loss. Moreover, some RCCs with a high intra-cystic protein content may mimic cystic pituitary adenoma, which makes their differential diagnosis ambiguous. Currently, medical professionals have no definitive way to distinguish RCCs from pituitary adenomas. Therefore, preoperative confirmation of RCCs would be of help to medical professionals for the management and proper surgical decision making. The goal of this study is to identify molecular markers in RCCs. Methods: We characterized aqueous and chloroform extracts of surgically resected RCCs and pituitary adenomas using ex vivo 1H NMR spectroscopy. Results: All RCCs exclusively showed the presence of mucopolysaccharides which are glycosaminoglycans (GAGs) made up of disaccharides of aminosugars and uronic sugars. Conclusion: GAGs can be used as metabolite marker for the detection of RCCs and this knowledge will lay the groundwork for the development of a non-invasive, in vivo magnetic resonance spectroscopy methodology for the differential diagnosis of RCCs and pituitary adenomas using clinical MRI scanners.

In Vivo and Ex Vivo Magnetic Resonance Spectroscopy in the Characterization of Hemangioblastoma Cyst Fluid

2014 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Girolamo Crisi ◽  
Silvano Filice ◽  
Thelma A. Pertinhez ◽  
Elisa Ventura ◽  
Franco Servadei
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Ex Vivo ◽  
Cyst Fluid ◽  
Resonance Spectroscopy

scholarly journals Metabolic Profiling of the Rat Liver After Chronic Ingestion of Alpha-Naphthylisothiocyanate Using In Vivo and Ex Vivo Magnetic Resonance Spectroscopy

2012 ◽  
Vol 126 (2) ◽  
pp. 306-316 ◽  
Author(s):  
B. S. Solanky ◽  
G. J. Sanchez-Canon ◽  
J. F. L. Cobbold ◽  
S. D. Taylor-Robinson ◽  
J. D. Bell ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Rat Liver ◽  
Metabolic Profiling ◽  
Ex Vivo ◽  
Resonance Spectroscopy ◽  
Chronic Ingestion

scholarly journals Hyperpolarized 13C pyruvate magnetic resonance spectroscopy for in vivo metabolic phenotyping of rat HCC

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elisabeth Bliemsrieder ◽  
Georgios Kaissis ◽  
Martin Grashei ◽  
Geoffrey Topping ◽  
Jennifer Altomonte ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Tumor Tissue ◽  
Ex Vivo ◽  
Kinetic Modelling ◽  
Lactate Production ◽  
Resonance Spectroscopy ◽  
Primary Tumors ◽  
Conversion Rates

AbstractThe in vivo assessment of tissue metabolism represents a novel strategy for the evaluation of oncologic disease. Hepatocellular carcinoma (HCC) is a high-prevalence, high-mortality tumor entity often discovered at a late stage. Recent evidence indicates that survival differences depend on metabolic alterations in tumor tissue, with particular focus on glucose metabolism and lactate production. Here, we present an in vivo imaging technique for metabolic tumor phenotyping in rat models of HCC. Endogenous HCC was induced in Wistar rats by oral diethyl-nitrosamine administration. Peak lactate-to-alanine signal ratios (L/A) were assessed with hyperpolarized magnetic resonance spectroscopic imaging (HPMRSI) after [1-13C]pyruvate injection. Cell lines were derived from a subset of primary tumors, re-implanted in nude rats, and assessed in vivo with dynamic hyperpolarized magnetic resonance spectroscopy (HPMRS) after [1-13C]pyruvate injection and kinetic modelling of pyruvate metabolism, taking into account systemic lactate production and recirculation. For ex vivo validation, enzyme activity and metabolite concentrations were spectroscopically quantified in cell and tumor tissue extracts. Mean peak L/A was higher in endogenous HCC compared to non-tumorous tissue. Dynamic HPMRS revealed higher pyruvate-to-lactate conversion rates (kpl) and lactate signal in subcutaneous tumors derived from high L/A tumor cells, consistent with ex vivo measurements of higher lactate dehydrogenase (LDH) levels in these cells. In conclusion, HPMRS and HPMRSI reveal distinct tumor phenotypes corresponding to differences in glycolytic metabolism in HCC tumor tissue.

scholarly journals P103 Metabolic profiling of the rat liver after chronic ingestion of  -naphthylisothiocyanate using in vivo and ex vivo magnetic resonance spectroscopy

Gut ◽  
2011 ◽  
Vol 60 (Suppl 2) ◽  
pp. A48-A48
Author(s):  
B. S. Solanky ◽  
G. J. Sanchez-Canon ◽  
J. F. L. Cobbold ◽  
S. D. Taylor-Robinson ◽  
J. D. Bell ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Rat Liver ◽  
Metabolic Profiling ◽  
Ex Vivo ◽  
Resonance Spectroscopy ◽  
Chronic Ingestion

scholarly journals In Vivo and Ex Vivo Magnetic Resonance Spectroscopy of the Infarct and the Subventricular Zone in Experimental Stroke

2015 ◽  
Vol 35 (5) ◽  
pp. 828-834 ◽  
Author(s):  
Elena Jiménez-Xarrié ◽  
Myriam Davila ◽  
Sara Gil-Perotín ◽  
Andrés Jurado-Rodríguez ◽  
Ana Paula Candiota ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Subventricular Zone ◽  
Ex Vivo ◽  
Fold Increase ◽  
Resonance Spectroscopy ◽  
Magic Angle ◽  
Metabolic Pattern

Ex vivo high-resolution magic-angle spinning (HRMAS) provides metabolic information with higher sensitivity and spectral resolution than in vivo magnetic resonance spectroscopy (MRS). Therefore, we used both techniques to better characterize the metabolic pattern of the infarct and the neural progenitor cells (NPCs) in the ipsilateral subventricular zone (SVZi). Ischemic stroke rats were divided into three groups: G0 (non-stroke controls, n = 6), G1 (day 1 after stroke, n = 6), and G7 (days 6 to 8 after stroke, n =12). All the rats underwent MRS. Three rats per group were analyzed by HRMAS. The remaining rats were used for immunohistochemical studies. In the infarct, both techniques detected significant metabolic changes. The most relevant change was in mobile lipids (2.80 ppm) in the G7 group (a 5.53- and a 3.95-fold increase by MRS and HRMAS, respectively). In the SVZi, MRS did not detect any significant metabolic change. However, HRMAS detected a 2.70-fold increase in lactate and a 0.68-fold decrease in N-acetylaspartate in the G1 group. None of the metabolites correlated with the 1.37-fold increase in NPCs detected by immunohistochemistry in the G7 group. In conclusion, HRMAS improves the metabolic characterization of the brain in experimental ischemic stroke. However, none of the metabolites qualifies as a surrogate biomarker of NPCs.

scholarly journals Intramyocardial triglyceride quantification by magnetic resonance spectroscopy: In vivo and ex vivo correlation in human subjects

2011 ◽  
Vol 65 (5) ◽  
pp. 1234-1238 ◽  
Author(s):  
Robert D. O'Connor ◽  
Jian Xu ◽  
Gregory A. Ewald ◽  
Joseph J. H. Ackerman ◽  
Linda R. Peterson ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Ex Vivo ◽  
Human Subjects ◽  
Resonance Spectroscopy
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