In Vivo and Ex Vivo Magnetic Resonance Spectroscopy in the Characterization of Hemangioblastoma Cyst Fluid

2014 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Girolamo Crisi ◽  
Silvano Filice ◽  
Thelma A. Pertinhez ◽  
Elisa Ventura ◽  
Franco Servadei
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Ex Vivo ◽  
Cyst Fluid ◽  
Resonance Spectroscopy

scholarly journals Proton Magnetic Resonance Spectroscopy Characterization of Rathke’s Cleft Cysts (RCCs): Relevance to the Differential Diagnosis of Pituitary Adenomas and RCCs

Cancers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 360
Author(s):  
Omkar B. Ijare ◽  
Martyn A. Sharpe ◽  
David S. Baskin ◽  
Kumar Pichumani
Keyword(s):  
Differential Diagnosis ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Pituitary Adenomas ◽  
Ex Vivo ◽  
Medical Professionals ◽  
Resonance Spectroscopy ◽  
Rathke's Cleft Cysts ◽  
Rathke's Cleft

Background: Rathke’s Cleft Cysts (RCCs) are rare epithelial cysts arising from remnants of the Rathke pouch in the pituitary gland. A subset of these lesions enlarge and produce a mass effect with consequent hypopituitarism, and may result in visual loss. Moreover, some RCCs with a high intra-cystic protein content may mimic cystic pituitary adenoma, which makes their differential diagnosis ambiguous. Currently, medical professionals have no definitive way to distinguish RCCs from pituitary adenomas. Therefore, preoperative confirmation of RCCs would be of help to medical professionals for the management and proper surgical decision making. The goal of this study is to identify molecular markers in RCCs. Methods: We characterized aqueous and chloroform extracts of surgically resected RCCs and pituitary adenomas using ex vivo 1H NMR spectroscopy. Results: All RCCs exclusively showed the presence of mucopolysaccharides which are glycosaminoglycans (GAGs) made up of disaccharides of aminosugars and uronic sugars. Conclusion: GAGs can be used as metabolite marker for the detection of RCCs and this knowledge will lay the groundwork for the development of a non-invasive, in vivo magnetic resonance spectroscopy methodology for the differential diagnosis of RCCs and pituitary adenomas using clinical MRI scanners.

scholarly journals Characterization of the Testicular Cell Types Present in the Rat by in Vivo 31P Magnetic Resonance Spectroscopy

1991 ◽  
Vol 45 (1) ◽  
pp. 122-127 ◽  
Author(s):  
Jeroen Van Der Grond ◽  
Ans M. M. Van Pelt ◽  
Cees J. A. Van Echteld ◽  
Grietje Dukstra ◽  
J. Anton Grootegoed ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Cell Types ◽  
Resonance Spectroscopy ◽  
Testicular Cell ◽  
31P Magnetic Resonance Spectroscopy

1H magnetic resonance spectroscopy of preinvasive and invasive cervical cancer: In vivo-ex vivo profiles and effect of tumor load

2004 ◽  
Vol 19 (3) ◽  
pp. 356-364 ◽  
Author(s):  
Marrita M. Mahon ◽  
I. Jane Cox ◽  
Roberto Dina ◽  
W. Patrick Soutter FRCOG ◽  
G. Angus McIndoe MRCOG ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Ex Vivo ◽  
Invasive Cervical Cancer ◽  
Resonance Spectroscopy ◽  
1H Magnetic Resonance Spectroscopy ◽  
Tumor Load

scholarly journals Characterization of Carbonic Anhydrase In Vivo Using Magnetic Resonance Spectroscopy

2020 ◽  
Vol 21 (7) ◽  
pp. 2442
Author(s):  
Jyoti Singh Tomar ◽  
Jun Shen
Keyword(s):  
Magnetic Resonance ◽  
Carbonic Anhydrase ◽  
Magnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Carbonic Anhydrases ◽  
Magnetization Saturation ◽  
Wide Range ◽  
In Vivo Characterization

Carbonic anhydrase is a ubiquitous metalloenzyme that catalyzes the reversible interconversion of CO2/HCO3−. Equilibrium of these species is maintained by the action of carbonic anhydrase. Recent advances in magnetic resonance spectroscopy have allowed, for the first time, in vivo characterization of carbonic anhydrase in the human brain. In this article, we review the theories and techniques of in vivo 13C magnetization (saturation) transfer magnetic resonance spectroscopy as they are applied to measuring the rate of exchange between CO2 and HCO3− catalyzed by carbonic anhydrase. Inhibitors of carbonic anhydrase have a wide range of therapeutic applications. Role of carbonic anhydrases and their inhibitors in many diseases are also reviewed to illustrate future applications of in vivo carbonic anhydrase assessment by magnetic resonance spectroscopy.

scholarly journals Metabolic Profiling of the Rat Liver After Chronic Ingestion of Alpha-Naphthylisothiocyanate Using In Vivo and Ex Vivo Magnetic Resonance Spectroscopy

2012 ◽  
Vol 126 (2) ◽  
pp. 306-316 ◽  
Author(s):  
B. S. Solanky ◽  
G. J. Sanchez-Canon ◽  
J. F. L. Cobbold ◽  
S. D. Taylor-Robinson ◽  
J. D. Bell ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Rat Liver ◽  
Metabolic Profiling ◽  
Ex Vivo ◽  
Resonance Spectroscopy ◽  
Chronic Ingestion

scholarly journals Hyperpolarized 13C pyruvate magnetic resonance spectroscopy for in vivo metabolic phenotyping of rat HCC

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elisabeth Bliemsrieder ◽  
Georgios Kaissis ◽  
Martin Grashei ◽  
Geoffrey Topping ◽  
Jennifer Altomonte ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Tumor Tissue ◽  
Ex Vivo ◽  
Kinetic Modelling ◽  
Lactate Production ◽  
Resonance Spectroscopy ◽  
Primary Tumors ◽  
Conversion Rates

AbstractThe in vivo assessment of tissue metabolism represents a novel strategy for the evaluation of oncologic disease. Hepatocellular carcinoma (HCC) is a high-prevalence, high-mortality tumor entity often discovered at a late stage. Recent evidence indicates that survival differences depend on metabolic alterations in tumor tissue, with particular focus on glucose metabolism and lactate production. Here, we present an in vivo imaging technique for metabolic tumor phenotyping in rat models of HCC. Endogenous HCC was induced in Wistar rats by oral diethyl-nitrosamine administration. Peak lactate-to-alanine signal ratios (L/A) were assessed with hyperpolarized magnetic resonance spectroscopic imaging (HPMRSI) after [1-13C]pyruvate injection. Cell lines were derived from a subset of primary tumors, re-implanted in nude rats, and assessed in vivo with dynamic hyperpolarized magnetic resonance spectroscopy (HPMRS) after [1-13C]pyruvate injection and kinetic modelling of pyruvate metabolism, taking into account systemic lactate production and recirculation. For ex vivo validation, enzyme activity and metabolite concentrations were spectroscopically quantified in cell and tumor tissue extracts. Mean peak L/A was higher in endogenous HCC compared to non-tumorous tissue. Dynamic HPMRS revealed higher pyruvate-to-lactate conversion rates (kpl) and lactate signal in subcutaneous tumors derived from high L/A tumor cells, consistent with ex vivo measurements of higher lactate dehydrogenase (LDH) levels in these cells. In conclusion, HPMRS and HPMRSI reveal distinct tumor phenotypes corresponding to differences in glycolytic metabolism in HCC tumor tissue.

scholarly journals P103 Metabolic profiling of the rat liver after chronic ingestion of  -naphthylisothiocyanate using in vivo and ex vivo magnetic resonance spectroscopy

Gut ◽  
2011 ◽  
Vol 60 (Suppl 2) ◽  
pp. A48-A48
Author(s):  
B. S. Solanky ◽  
G. J. Sanchez-Canon ◽  
J. F. L. Cobbold ◽  
S. D. Taylor-Robinson ◽  
J. D. Bell ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Rat Liver ◽  
Metabolic Profiling ◽  
Ex Vivo ◽  
Resonance Spectroscopy ◽  
Chronic Ingestion
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