IMP3 Protein Overexpression Is Linked to Unfavorable Outcome in Laryngeal Squamous Cell Carcinoma

Background: The aim of this study was to (i) determine IMP3 protein expression in benign and malignant laryngeal lesions, (ii) compare its expression to Ki-67, p53, cyclin D1, and (iii) finally, to examine the prognostic power of IMP3 in squamous cell carcinomas of the larynx (LSSC). Methods: IMP3 protein expression was evaluated in 145 patients, including 62 LSCC, 45 dysplasia (25 with low and 20 with high-grade dysplasia), and 38 benign lesions (vocal cord polyps and nodules). Results: IMP3 was significantly higher expressed in LSCC compared to dysplasia and benign lesions (p < 0.001; p < 0.001, respectively). Similarly, higher expression patterns were observed for Ki-67 and p53, whereas cyclin D1 was equally distributed in all three lesions. IMP3 (p = 0.04) and Ki-67 (p = 0.02) expressions were significantly linked to neck node positivity, and IMP3 overexpression to worse disease-specific survival (p = 0.027). Conclusion: Since IMP3 showed significantly higher expression in laryngeal carcinomas, but not in high- or low-grade dysplasia, it serves as a useful marker to differentiate between invasive and noninvasive lesions. Higher IMP3 expression represented a significantly worse prognosticator for clinical outcomes of patients with squamous cell carcinoma of the larynx.

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Expression of cyclin D1 and Ki-67 in squamous cell carcinoma of the oral cavity: clinicopathological and prognostic significance

Oral Oncology ◽

10.1016/s1368-8375(01)00060-4 ◽

2002 ◽

Vol 38 (3) ◽

pp. 301-308 ◽

Cited By ~ 49

Author(s):

Juan Carlos de Vicente ◽

Agustı́n Herrero-Zapatero ◽

Manuel Florentino Fresno ◽

Juan Sebastián López-Arranz

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Cavity ◽

Cell Carcinoma ◽

Cyclin D1 ◽

Squamous Cell ◽

Prognostic Significance ◽

Ki 67

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Cyclin D1 and Ki-67 expression correlates to tumor staging in tongue squamous cell carcinoma

Medicina Oral Patología Oral y Cirugia Bucal ◽

10.4317/medoral.20601 ◽

2015 ◽

pp. e657-e663 ◽

Cited By ~ 7

Author(s):

EP. Guimaraes ◽

ML. de Carli ◽

FF. Sperandio ◽

JAC. Hanemann ◽

AAC. Pereira

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Cyclin D1 ◽

Squamous Cell ◽

Tumor Staging ◽

Tongue Squamous Cell Carcinoma ◽

Ki 67

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Clinical significance of STAT3 and MAPK phosphorylation, and the protein expression of cyclin D1 in skin squamous cell carcinoma tissues

Molecular Medicine Reports ◽

10.3892/mmr.2015.4460 ◽

2012 ◽

Vol 12 (6) ◽

pp. 8129-8134 ◽

Cited By ~ 3

Author(s):

JIAN WU ◽

WEN-YING LU ◽

LEI-LEI CUI

Keyword(s):

Squamous Cell Carcinoma ◽

Protein Expression ◽

Cell Carcinoma ◽

Cyclin D1 ◽

Clinical Significance ◽

Squamous Cell ◽

Mapk Phosphorylation ◽

Skin Squamous Cell Carcinoma

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Expression of proliferation markers in laryngeal squamous cell carcinoma: an association with clinico-pathological factors and treatment outcomes

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20201675 ◽

2020 ◽

Vol 6 (5) ◽

pp. 858

Author(s):

Elizabeth Mathew Iype ◽

Rajan Balakrishna ◽

Lakshmi Subhadradevi ◽

Jissa Vinoda Thulaseedharan ◽

Bharath Veerabadhran ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Cyclin D1 ◽

Treatment Outcomes ◽

Squamous Cell ◽

Laryngeal Squamous Cell Carcinoma ◽

Ki 67 ◽

Proliferation Markers ◽

Tissue Samples ◽

Cyclin Dl

Background: The study was done with the objective to study the expression of epidermal growth factor receptor (EGFR), cyclin D1 and Ki-67 in laryngeal squamous cell carcinoma and to assess the correlation of all three proliferation markers with various clinic-pathological parameters, the treatment outcomes as well as survival.Methods: We prospectively evaluated the surgical specimens of 72 laryngeal squamous cell carcinoma (LSCC) patients, treated with primary surgery and post-operative adjuvant therapy. Tumor tissue samples were analysed for the expression of EGFR, cyclin D1 and Ki-67 markers and analysis were done by immune-histochemistry and western blot test. Results: EGFR showed significant expression in 67.6% and was insignificant in 31.9% patients in our analysis of 72 tumor samples. Cyclin D1 showed intense expression in 43%, and was insignificant in 57% patients. Ki-67 was intensely expressed in 43% patients. There was no correlation between expression of these markers with age, T-stage and N-stage. However, all the three markers showed significantly intense expression in tumours with extra capsular disease as well as perineural invasion (PNI) both of which are features of invasiveness of the tumor.Conclusions: Estimation of biomarkers such as EGFR, cyclin Dl, and Ki-67 could be beneficial in predicting tumor aggressiveness, prognosis and survival in LSCC patients. Thus, all the three proliferation markers can be categorized as markers of invasiveness. Combination of proliferation markers-EGFR, cyclin D1 and Ki-67 is useful pre-operatively in planning surgical strategies so as to decide a more radical approach for the resection of the primary as well as neck dissection.

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Selected molecular markers as indicators of clinical profile, tumor characteristics and treatment outcome in squamous cell carcinoma of the larynx.

Journal of Clinical Oncology ◽

10.1200/jco.2019.37.15_suppl.e17522 ◽

2019 ◽

Vol 37 (15_suppl) ◽

pp. e17522-e17522

Author(s):

Elizabeth Mathew Iype ◽

Lakshmi S ◽

Rajan Balakrishnan

Keyword(s):

Squamous Cell Carcinoma ◽

Molecular Markers ◽

Cell Carcinoma ◽

Cyclin D1 ◽

Squamous Cell ◽

Laryngeal Squamous Cell Carcinoma ◽

Ki 67 ◽

Proliferation Markers ◽

Node Negative ◽

Cox 2

e17522 Background: Laryngeal Squamous cell carcinoma is widely prevalent in India and is the fourth leading cancer in males. The possibility that specific tumour characteristics (demonstrable by immunohistochemistry) may aid the clinician in choosing optimal treatments for a specific tumour, holds great promise. Objectives of the present study were to study the expression of select molecular markers p53, Bcl-2 (Apoptotic markers), EGFR, Ki67, Cyclin D1 (Proliferation markers) and Cox-2 (Inflammatory marker) in LSCC so as to form a hypothesis that a particular group of markers will be useful in determining the prognosis of LSCC. Methods: We studied 72 cases of laryngeal squamous cell carcinoma in an attempt to determine relationship between their clinicopathological characteristics and treatment outcome with the six molecular markers. Samples from patients who underwent laryngectomy from Jan 2006 to Dec 2010 were taken and analyzed prospectively and followed up till Dec 2015 or till death. . Immunoreactivity in tissue sections was evaluated as negative when no positive cells were observed within the tumor, weak (1+) , moderate (2+) , and strong or intense (3+) . Data were analyzed using SPSS software package. Chi squared test or exact test were used to test the associations. Logistic regression analysis was used to estimate the odds ratios and 95% confidence intervals. The disease free survival time of each patient was calculated by taking the difference between the date of surgery and the recurrence date/ death date/ the last follow up date whichever is applicable. Results: Proliferation markers EGFR, Cyclin D1 and Ki 67, individually and collectively were predictive of extracapsular spread and perineural spread of tumour. Node negative patients having intense expression of Cyclin D1 or Ki 67 has bad prognosis.. The significant expression of Cox-2 was highly predictive of Node positivity. In older patients and in T4 stage the presence of P53 / Bcl-2 resulted in worse overall survival. Markers of aggressiveness were identified as p53, Bcl-2 Cox-2. Markers of invasiveness were EGFR, Cyclin D1 and Ki 67. Markers predicting survival were p53, BCl-2, Cyclin D1 and Ki 67. Conclusions: If Proliferation markers are detected preoperatively in biopsy specimens, has important implications in planning aggressive management Cyclin D1 or Ki 67 in Node negative patients indicate need for a neck dissection or irradiation. Cox-2 assessment will be useful in predicting the occult nodal metastasis for prophylactic treatment

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P032 Differential Protein Expression Patterns of p53, NF-κB, and NF-κB– Regulated Genes in Head and Neck Squamous Cell Carcinoma

Archives of Otolaryngology - Head and Neck Surgery ◽

10.1001/archotol.132.8.871-b ◽

2006 ◽

Vol 132 (8) ◽

pp. 871

Author(s):

P. Duggal ◽

Z. Chen ◽

C. Van Waes

Keyword(s):

Squamous Cell Carcinoma ◽

Protein Expression ◽

Cell Carcinoma ◽

Head And Neck ◽

Squamous Cell ◽

Expression Patterns ◽

Neck Squamous Cell Carcinoma ◽

Differential Protein Expression ◽

Differential Protein

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Defining Molecular Phenotypes of Human Papillomavirus–Associated Oropharyngeal Squamous Cell Carcinoma

Otolaryngology ◽

10.1016/j.otohns.2009.04.014 ◽

2009 ◽

Vol 141 (3) ◽

pp. 382-389 ◽

Cited By ~ 35

Author(s):

Paul M. Weinberger ◽

Ziwei Yu ◽

Panteleimon Kountourakis ◽

Clarence Sasaki ◽

Bruce G. Haffty ◽

...

Keyword(s):

Cervical Cancer ◽

Squamous Cell Carcinoma ◽

Human Papillomavirus ◽

Growth Factor ◽

Protein Expression ◽

Cell Carcinoma ◽

Squamous Cell ◽

Expression Patterns ◽

Molecular Phenotypes ◽

P16 Expression

OBJECTIVE: The purpose of this study was to determine if oropharyngeal squamous cell carcinoma (OSCC) classified into three groups based on human papillomavirus (HPV) 16 DNA presence and p16 expression display different protein expression patterns. STUDY DESIGN: Cross-sectional study. SETTING: A laboratory-based study of patients with OSCC treated at a tertiary care academic medical center. SUBJECTS AND METHODS: Paraffin-embedded OSCC specimens from 77 patients classified into the three-class model (HPV negative, HPV inactive [HPV16+/p16–], and HPV active [HPV16+/p16 +]) were queried for the expression of 14 tumor progression proteins using AQUA (HistoRx, New Haven CT). Protein expression between groups was assessed by analysis of variance. Global expression patterns were determined by unsupervised hierarchical clustering. RESULTS: There were significant differences in expression of β-catenin ( P = 0.009), epidermal growth factor receptor ( P = 0.009), and vascular endothelial growth factor ( P = 0.028) between groups. HPV-active tumors had overexpression of β-catenin. Hierarchical clustering showed HPV-negative and HPV-inactive tumors displayed association patterns distinct from HPV-active tumors. CONCLUSIONS: Tumors classified by HPV DNA presence and p16 expression have different molecular phenotypes. This is the first demonstration of overexpression of β-catenin (also found in HPV-caused cervical cancer) in HPV-active OSCC. HPV-active OSCC may share a similar ontogeny to HPV-caused cervical cancer.

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