Alterations in Immune Response Profile of Tumor-Draining Lymph Nodes after High-Intensity Focused Ultrasound Ablation of Breast Cancer Patients

Previous studies have revealed that high-intensity focused ultrasound (HIFU) ablation can trigger an antitumor immune response. The aim of this study was to investigate immune response in tumor-draining lymph nodes (TDLNs) after HIFU treatment. Forty-eight female patients with biopsy-confirmed breast cancer were divided into a control group and an HIFU group. In the control group, 25 patients underwent modified radical mastectomy, but 23 patients in the HIFU group received HIFU ablation of primary cancer, followed by the same operation. Using HE and immunohistochemical staining, the immunologic reactivity pattern and immune cell profile were assessed in paraffin-embedded axillary lymph nodes (ALNs) in all patients. The results showed that ALNs presented more evident immune reactions in the HIFU group than in the control group (100% vs. 64%). Among the ALNs, 78.3% had mixed cellular and humoral immune response, whereas 36% in the control group showed cellular immune response. The numbers of CD3+, CD4+, NK cell, and activated CTLs with Fas ligand+, granzyme+ and perforin+ expression were significantly higher in the ALNs in the HIFU group. It was concluded that HIFU could stimulate potent immune response and significantly increase T cell, activated CTLs and NK cell populations in the TDLNs of breast cancer.

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Immune response after high-intensity focused ultrasound ablation for H22 tumor

Journal of Clinical Oncology ◽

10.1200/jco.2007.25.18_suppl.21169 ◽

2007 ◽

Vol 25 (18_suppl) ◽

pp. 21169-21169 ◽

Cited By ~ 2

Author(s):

P. Zhou ◽

M. Fu ◽

J. Bai ◽

Z. Wang ◽

F. Wu

Keyword(s):

Immune Response ◽

Tumor Immunity ◽

High Intensity ◽

Focused Ultrasound ◽

Survival Rates ◽

High Intensity Focused Ultrasound ◽

Control Group ◽

Term Survival ◽

Hifu Ablation ◽

Significant Difference

21169 Background: Previous results have shown that high intensity focused ultrasound (HIFU) ablation can potentially activate a host anti-tumor immunity. This study was to investigate whether acoustic cavitaion may enhance host immune responses after HIFU treatment for solid malignancy, and to explore the potential mechanisms regarding the enhanced anti-tumor immunity. Methods: (1) Tumor cell vaccine preparation: H22 cells were irradiated with HIFU (0.8MHz, 1050W/cm2, 90s); H22 cells were heated for one hour in 65°C water bath. (2) Animal study: 150 KM mice were randomly divided into three groups: control group, thermal group, and HIFU group. Each group had 50 mice for immune experiments. By using hypodermic injection, the mice in thermal group and HIFU group received either 0.2ml heat-treated H22 vaccine or 0.2ml HIFU-treated H22 vaccine in the left flank of each mouse. Those in control group received only injection with same amount of saline solution. The vaccination times were 4 sessions, once a week for 4 consecutive weeks. One week after last vaccination, each mouse was challenged with H22 tumor cells. All mice were followed up to observe the long-term survival in each group, and peripheral blood was collected to detect changes in T lymphocytes and their subsets by flow cytometry technique. Results: The tumor incidences and 6-week survival rates were observed 100% and 88% of mice in control group, 72.5% and 40% in thermal group, and 42.5% and 14% in HIFU group respectively. Compared to the values in either control group or thermal group, there was a significant decrease of tumor incidences and 6-week survival rates in HIFU group. The volume of tumor was obviously lower in HIFU group, and a significant difference was observed between HIFU group and thermal group or control group. Compared to the values in control group, there were a significant increase of CD4+ levels and CD4+/CD8+ ratio, and a significant decrease of CD8+ level in both HIFU group and thermal group. Conclusions: Antitumor immune response could be enhanced after HIFU ablation for H22 implanted tumor in mice, and acoustic cavitation could play an important role to stimulate host antitumor immune system. No significant financial relationships to disclose.

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Histologische Morphologie von Läsionen nach MR-gesteuerter high-intensity focused ultrasound (MR-HIFU)-Ablation: in-vitro Untersuchung an Putenbrust

RöFo - Fortschritte auf dem Gebiet der Röntgenstrahlen und der bildgebenden Verfahren ◽

10.1055/s-0030-1268301 ◽

2010 ◽

Vol 182 (11) ◽

Author(s):

M Karul ◽

A Neumann ◽

H Merz ◽

J Barkhausen ◽

P Hunold

Keyword(s):

High Intensity ◽

Focused Ultrasound ◽

High Intensity Focused Ultrasound ◽

Hifu Ablation

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A 2-Fold Interpenetrating Zn(II)-MOF: Sensitive Detection of Fe3+ Ion and Anti-Cancer Activity on Breast Cancer Through high Intensity Focused Ultrasound

Journal of Inorganic and Organometallic Polymers and Materials ◽

10.1007/s10904-021-01921-6 ◽

2021 ◽

Vol 31 (5) ◽

pp. 2209-2217

Author(s):

Jing Huang ◽

Xiao-Yan Pu ◽

Zheng Liu ◽

Xiang-Yun Cao ◽

Jian Fu

Keyword(s):

Breast Cancer ◽

High Intensity ◽

Focused Ultrasound ◽

High Intensity Focused Ultrasound ◽

Sensitive Detection ◽

Anti Cancer ◽

Cancer Activity

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High-intensity focused ultrasonic ablation of breast cancer

Research n Practical Medicine Journal ◽

10.17709/2409-2231-2018-5-3-6 ◽

2018 ◽

Vol 5 (3) ◽

pp. 67-76

Author(s):

L. I. Moskvicheva

Keyword(s):

Breast Cancer ◽

High Intensity ◽

Focused Ultrasound ◽

Malignant Tumors ◽

High Intensity Focused Ultrasound ◽

Ultrasound Therapy ◽

Technical Success ◽

Short Term ◽

Technical Safety ◽

Complete Tumor

Breast cancer is the most frequent and socially signiﬁ cant malignant tumor disease of the female popula on of the Russian Federa on. At present, for the treatment of this pathology, the methods of the organ-preserving surgical manual allowing to achieve the maximum cosme c eﬀ ect along with the observance of the principles of cancer safety are ge ng more and more developed,. In recent decades, researchers from diﬀ erent countries demonstrate the feasibility and technical safety of various mini-invasive methods of thermal abla on of early stages of breast cancer, characterized by rela ve simplicity of implementa on, excellent cosme c results, short-term rehabilita on of pa ents. These include hyperthermic techniques (radiofrequency, microwave, laser abla on) and hypothermic method (cryoabla on). Each technique has unique characteris cs of impact on the tumor and is performed under the control of ultrasound, computer tomography or magne c resonance imaging. The technical success of these abla on techniques is achieved in 93–98% of cases. Complete tumor necrosis a er radiofrequency abla on is observed in 76–100% of pa ents with breast cancer, laser abla on – in 13–76%, microwave abla on – in 0–8%, cryoabla on – in 36–83%. The frequency of development of speciﬁ c complica ons of local thermal destruc on is 4–13%. To date, there is only one method of noninvasive extracorporeal thermal abla on of tumors of diﬀ erent localiza on – high-intensity focused ultrasound therapy, the eﬀ ec veness and safety of which as a method of local destruc on of benign and malignant tumors of the breast demonstrated by many authors. Like all methods of thermal abla on, high-intensity focused ultrasound therapy has its limita ons, complica ons and disadvantages. This ar cle presents a literary review that highlights the possibili es of this method of local destruc on in pa ents with malignant tumors of the breast.

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