scholarly journals Identification of Astrocytoma Blood Serum Protein Profile

Cells ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 16
Author(s):  
Paulina Vaitkiene ◽  
Ruta Urbanaviciute ◽  
Povilas Grigas ◽  
Giedrius Steponaitis ◽  
Arimantas Tamasauskas ◽  
...  
Keyword(s):  
Decision Tree ◽  
Blood Serum ◽  
Serum Protein ◽  
Serum Proteins ◽  
Protein Profile ◽  
Signs And Symptoms ◽  
Diagnostic Tools ◽  
Blood Serum Proteins ◽  
Blood Serum Protein ◽  
Diagnostic Applications

High-grade astrocytomas are some of the most common and aggressive brain cancers, whose signs and symptoms are initially non-specific. Up to the present date, there are no diagnostic tools to observe the early onset of the disease. Here, we analyzed the combination of blood serum proteins, which may play key roles in the tumorigenesis and the progression of glial tumors. Fifty-nine astrocytoma patients and 43 control serums were analyzed using Custom Human Protein Antibody Arrays, including ten targets: ANGPT1, AREG, IGF1, IP10, MMP2, NCAM1, OPN, PAI1, TGFβ1, and TIMP1. The decision tree analysis indicates that serums ANGPT1, TIMP1, IP10, and TGFβ1 are promising combinations of targets for glioma diagnostic applications. The accuracy of the decision tree algorithm was 73.5% (75/102), which correctly classified 79.7% (47/59) astrocytomas and 65.1% (28/43) healthy controls. The analysis revealed that the relative value of osteopontin (OPN) protein level alone predicted the 12-month survival of glioblastoma (GBM) patients with the specificity of 84%, while the inclusion of the IP10 protein increased model predictability to 92.3%. In conclusion, the serum protein profiles of ANGPT1, TIMP1, IP10, and TGFβ1 were associated with the presence of astrocytoma independent of its malignancy grade, while OPN and IP10 were associated with GBM patient survival.

Notizen: Quantification and Localisation of SH-Groups in Human Blood Serum Proteins

1978 ◽  
Vol 33 (9-10) ◽  
pp. 803-805 ◽  
Author(s):  
E. Schauenstein ◽  
F. Dachs
Keyword(s):  
Blood Serum ◽  
Serum Protein ◽  
Human Blood ◽  
Serum Proteins ◽  
Total Activity ◽  
Expected Value ◽  
Human Blood Serum ◽  
Thiol Groups ◽  
Disulfide Exchange ◽  
Blood Serum Proteins

Abstract The thiol groups of human blood serum proteins were determined after 24 hours interaction with dithionitrobenzoicacid (DTNB) to an average of 538 ± 60 µmol/l serum. After treatment of the serum with [35S]DTNB , autoradiograms of the protein elpherograms revealed two main peaks: The first with 63% of total activity, in the albumin region, corresponding to 0.60 SH/mol, the second with 23% of total activity, in the 7-globulin range, corresponding to 2.2 SH/mol. After 30 minutes incubation with D TNB , or with p-chloromercuribenzoate (CMB), in freshly prepared pools of IgG only 0.2 SH/mol were found which is the expected value already known from the literature.Autoradiograms taken from serum protein elpherograms after interaction with [UC] CMB only show the main SH-peak in the albumin range. Thus ist is concluded that the SH-peak in the γ-globulin region after 24 hours incubation with [35S]DTNB is due to one highly labile S-S-bond which easily undergoes a disulfide exchange with DTNB .

scholarly journals Blood serum protein in periparturient goats supplemented with various forms of zinc

2016 ◽  
Vol 85 (4) ◽  
pp. 387-394 ◽  
Author(s):  
Lenka Kudělková ◽  
Leoš Pavlata ◽  
Alena Pechová ◽  
Jaroslav Filípek
Keyword(s):  
Blood Serum ◽  
Serum Protein ◽  
Serum Proteins ◽  
Protein Profile ◽  
Electrophoretic Analysis ◽  
Positive Influence ◽  
Protein Fractions ◽  
Negative Effects ◽  
Individual Protein ◽  
Group B

The aim of the study was to compare the contents of individual protein fractions determined by electrophoresis in the blood serum of 32 periparturient goats supplemented with various forms of zinc (Zn). Goats were divided into four groups: Group A was a control (without Zn supplementation). The remaining three groups (B, C, D) were supplemented with Zn in various forms. Group B was supplemented with zinc oxide, Group C with zinc lactate, and Group D with zinc chelate. Individual protein fractions (albumins, α1, α2, β1, β2 a γ-globulins) were identified by electrophoretic analysis. All supplemented groups showed the trend of a positive influence of Zn on γG fractions in the serum protein of the goats; this tendency was significant only in Group C. Moreover, Group C had significantly the highest total protein values and albumins. The results indicated that a feed enriched with various forms of Zn had a significant influence on the quantity of individual protein fractions of blood serum. Our results provide new knowledge on the values of individual protein fractions of serum goats and should be taken into consideration when interpreting the serum protein profile with regard to periparturient goats. Furthermore, we showed the possible positive or negative effects on the values of individual fraction on serum proteins with organic and inorganic zinc form supplemented rich in diet.

scholarly journals HAEMATOLOGICAL STUDIES (IN THE GUINEA FOWL (Numida rneteagris (Pallas): H. EFFECT OF AGE, SEX AND TIME OF BLEEDING ON PROTEIN AND ELECTROLYTE LEVELS IN BLOOD SERUM OF GUINEA FOWLS.

2021 ◽  
Vol 13 ◽  
pp. 102-107
Author(s):  
B. I. Orji ◽  
G. C. Okeke ◽  
O. O. Ojo
Keyword(s):  
Blood Serum ◽  
Serum Protein ◽  
Serum Proteins ◽  
Guinea Fowl ◽  
Factorial Experiment ◽  
Blood Serum Protein ◽  
Effect Of Age

The blood serum protein and electrolyte levels as well as the effects of age, sex and time of bleeding on these were investigated in a 2x2x2 factorial experiment using twenty four mature guinea fowls(Numedia melagris pallas). The results showed fiat the serum proteins and major electrolyte levels — sodium . (ppm), potassium. (ppm) calcium (mg/100 ml), magkesium (mg/100 ml) and phosphates mg/100ml) were res­pectively 2.45 ±0.04, 3231 56 ±107.52, 1301. 26 ±33.01, 14.16+0.0.54, 4.18+0.10 and 6.43 -_+_0.22. Sex, time of bleeding and some­times age and their interactions had signifi­cant effect on the levels of these parameters in blood, Correlations between these were also calculated.

Blood serum proteins of squirrel monkey (Saimiri sciurea)

Primates ◽  
1972 ◽  
Vol 13 (3) ◽  
pp. 243-249
Author(s):  
H. A. Annenkov
Keyword(s):  
Blood Serum ◽  
Squirrel Monkey ◽  
Serum Proteins ◽  
Blood Serum Proteins ◽  
Saimiri Sciurea

Separation of Blood Serum Proteins by Ultrafiltration

1970 ◽  
pp. 139-149 ◽  
Author(s):  
Carel J. van Oss ◽  
Paul M. Bronson
Keyword(s):  
Blood Serum ◽  
Serum Proteins ◽  
Blood Serum Proteins

Electrophoretic Studies on Blood Serum Proteins of Rodents

1960 ◽  
Vol 41 (3) ◽  
pp. 405 ◽  
Author(s):  
A. H. Auernheimer ◽  
William Cutter ◽  
F. O. Atchley
Keyword(s):  
Blood Serum ◽  
Serum Proteins ◽  
Blood Serum Proteins
Sign in / Sign up

Export Citation Format

Share Document