scholarly journals Toll-Like Receptor 2 Expression as a New Hallmark of Advanced Endometriosis

Cells ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1813 ◽  
Author(s):  
Małgorzata Sobstyl ◽  
Paulina Niedźwiedzka-Rystwej ◽  
Ewelina Grywalska ◽  
Izabela Korona-Głowniak ◽  
Anna Sobstyl ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
B Lymphocytes ◽  
Characteristic Curve ◽  
Cost Effective ◽  
Peripheral Blood Lymphocytes ◽  
Toll Like Receptor ◽  
Myeloid Dendritic Cells ◽  
Tlr2 Expression ◽  
Toll Like Receptor 2 ◽  
Effective Diagnosis

Recent evidence suggests that immunological aspects play a pivotal role in this disorder. Toll-like receptor 2 (TLR2) is crucial in recognizing microbial infections and mediating innate immune response. The objective of our study was to rate with flow cytometry the levels of several subsets of dendritic cells, monocytes, and basic peripheral blood lymphocytes expressing TLR2, aiming at the determination of a possible correlation between the expression of TLR2 and the clinical outcomes of endometriosis in 40 patients and 40 age-matched healthy women. Our study showed the importance of TLR2 expression, mainly on myeloid dendritic cells (mDCs) and B cells in patients with endometriosis. Both mDCs BDCA1+CD19-TLR2+ and B lymphocytes CD19+TLR-2+ proved useful in the differentiation of affected individuals with stages 3–4 of the disease (area under the receiver operating characteristic curve /AUC/ = 0.96, p < 0.0001 for mDCs; AUC = 0.78, p = 0.0001 for B lymphocytes), and those presenting adhesion (AUC = 0.92, p < 0.0001 for mDCs; AUC = 0.82, p < 0.0001 for B lymphocytes) or infertility (AUC = 0.83, p < 0.0001 for mDCs; AUC = 0.73, p = 0.006 for B lymphocytes). Our findings suggest that the levels of TLR2-expressing cells, particularly mDCs and B lymphocytes, may be an effective biomarker of endometriosis, because the disease currently lacks clinically useful noninvasive biomarkers enabling early and cost-effective diagnosis.

Quantitative expression of Toll-like receptor-2, -4, and -9 in dendritic cells generated from blasts of patients with acute myeloid leukemia

Transfusion ◽  
2008 ◽  
Vol 48 (5) ◽  
pp. 861-870 ◽  
Author(s):  
Anita Schmitt ◽  
Li Li ◽  
Krzysztof Giannopoulos ◽  
Jochen Greiner ◽  
Peter Reinhardt ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Dendritic Cells ◽  
Myeloid Leukemia ◽  
Toll Like Receptor ◽  
Quantitative Expression ◽  
Toll Like Receptor 2 ◽  
Acute Myeloid

scholarly journals The Endoplasmic Reticulum-resident Heat Shock Protein Gp96 Activates Dendritic Cells via the Toll-like Receptor 2/4 Pathway

2002 ◽  
Vol 277 (23) ◽  
pp. 20847-20853 ◽  
Author(s):  
Ramunas M. Vabulas ◽  
Sibylla Braedel ◽  
Norbert Hilf ◽  
Harpreet Singh-Jasuja ◽  
Sylvia Herter ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Dendritic Cells ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Toll Like Receptor ◽  
Heat Shock Protein Gp96 ◽  
Toll Like Receptor 2

scholarly journals Macrophage and dendritic cell subset composition can distinguish endotypes in adjuvant-induced asthma mouse models

2021 ◽  
Author(s):  
Müge Özkan ◽  
Yusuf Cem Eskiocak ◽  
Gerhard Wingender
Keyword(s):  
Dendritic Cells ◽  
Dendritic Cell ◽  
Mouse Models ◽  
Cell Subset ◽  
Clear Understanding ◽  
Dendritic Cell Subset ◽  
Myeloid Dendritic Cells ◽  
Differential Distribution ◽  
Eosinophilic Asthma ◽  
Effective Diagnosis

Asthma is a heterogeneous disease with neutrophilic and eosinophilic asthma as the main endotypes that are distinguished according to the cells recruited to the airways and the related pathology. Eosinophilic asthma is the treatment-responsive endotype, which is mainly associated with allergic asthma. Neutrophilic asthma is a treatment-resistant endotype, affecting 5-10% of asthmatics. Although eosinophilic asthma is well-studied, a clear understanding of the endotypes is essential to devise effective diagnosis and treatment approaches for neutrophilic asthma. To this end, we directly compared adjuvant-induced mouse models of neutrophilic (CFA/OVA) and eosinophilic (Alum/OVA) asthma side-by-side. The immune response in the inflamed lung was analyzed by multi-parametric flow cytometry and immunofluorescence. We found that eosinophilic asthma was characterized by a preferential recruitment of interstitial macrophages and myeloid dendritic cells, whereas in neutrophilic asthma plasmacytoid dendritic cells, exudate macrophages, and GL7 + activated B cells predominated. This differential distribution of macrophage and dendritic cell subsets reveals important aspects of the pathophysiology of asthma and holds the promise to be used as biomarkers to diagnose asthma endotypes.

scholarly journals Lactobacillus acidophilus induces virus immune defence genes in murine dendritic cells by a Toll-like receptor-2-dependent mechanism

Immunology ◽  
2010 ◽  
Vol 131 (2) ◽  
pp. 268-281 ◽  
Author(s):  
Gudrun Weiss ◽  
Simon Rasmussen ◽  
Louise Hjerrild Zeuthen ◽  
Birgit Nøhr Nielsen ◽  
Hanne Jarmer ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lactobacillus Acidophilus ◽  
Immune Defence ◽  
Toll Like Receptor ◽  
Defence Genes ◽  
Toll Like Receptor 2 ◽  
Dependent Mechanism

scholarly journals Toll-like Receptor 2 and DC-SIGNR1 Differentially Regulate Suppressors of Cytokine Signaling 1 in Dendritic Cells duringMycobacterium tuberculosisInfection

2009 ◽  
Vol 284 (38) ◽  
pp. 25532-25541 ◽  
Author(s):  
Varsha Srivastava ◽  
Mini Manchanda ◽  
Shashank Gupta ◽  
Rupak Singla ◽  
Digamber Behera ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cytokine Signaling ◽  
Toll Like Receptor ◽  
Toll Like Receptor 2 ◽  
Suppressors Of Cytokine Signaling

Toll-like receptor 4-dependent activation of myeloid dendritic cells by leukocidin of Staphylococcus aureus

2009 ◽  
Vol 11 (2) ◽  
pp. 245-253 ◽  
Author(s):  
Ken Inden ◽  
Jun Kaneko ◽  
Akiko Miyazato ◽  
Natsuo Yamamoto ◽  
Shota Mouri ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dendritic Cells ◽  
Toll Like Receptor ◽  
Myeloid Dendritic Cells ◽  
Toll Like Receptor 4

scholarly journals Group B Streptococcus and Streptococcus suis Capsular Polysaccharides Induce Chemokine Production by Dendritic Cells via Toll-Like Receptor 2- and MyD88-Dependent and -Independent Pathways

2013 ◽  
Vol 81 (9) ◽  
pp. 3106-3118 ◽  
Author(s):  
Cynthia Calzas ◽  
Guillaume Goyette-Desjardins ◽  
Paul Lemire ◽  
Fleur Gagnon ◽  
Claude Lachance ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Capsular Polysaccharides ◽  
Toll Like Receptor ◽  
Chemokine Production ◽  
Content Type ◽  
Toll Like Receptor 2 ◽  
Group B ◽  
Necrosis Factor

ABSTRACTStreptococcus agalactiae(also known as group BStreptococcus[GBS]) andStreptococcus suisare encapsulated streptococci causing severe septicemia and meningitis. Bacterial capsular polysaccharides (CPSs) are poorly immunogenic, but anti-CPS antibodies are essential to the host defense against encapsulated bacteria. The mechanisms underlying anti-CPS antibody responses are not fully elucidated, but the biochemistry of CPSs, particularly the presence of sialic acid, may have an immunosuppressive effect. We investigated the ability of highly purifiedS. suisand GBS native (sialylated) CPSs to activate dendritic cells (DCs), which are crucial actors in the initiation of humoral immunity. The influence of CPS biochemistry was studied using CPSs extracted from different serotypes within these two streptococcal species, as well as desialylated CPSs. No interleukin-1β (IL-1β), IL-6, IL-12p70, tumor necrosis factor alpha (TNF-α), or IL-10 production was observed inS. suisor GBS CPS-stimulated DCs. Moreover, these CPSs exerted immunosuppressive effects on DC activation, as a diminution of gamma interferon (IFN-γ)-induced B cell-activating factor of the tumor necrosis factor family (BAFF) expression was observed in CPS-pretreated cells. However,S. suisand GBS CPSs induced significant production of CCL3, via partially Toll-like receptor 2 (TLR2)- and myeloid differentiation factor 88 (MyD88)-dependent pathways, and CCL2, via TLR-independent mechanisms. No major influence of CPS biochemistry was observed on the capacity to induce chemokine production by DCs, indicating that DCs respond to these CPSs in a patterned way rather than a structure-dedicated manner.

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