scholarly journals Electrochemical Determination of Lead & Copper Ions Using Thiolated Calix[4]arene-Modified Screen-Printed Carbon Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 157
Author(s):  
Chong Jin Mei ◽  
Nor Azah Yusof ◽  
Shahrul Ainliah Alang Ahmad
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Copper Ions ◽  
Current Response ◽  
Carbon Electrode ◽  
Significant Drop ◽  
Screen Printed Carbon Electrode ◽  
Relative Standard ◽  
Competitive Ions ◽  
Screen Printed

This study used a thiolated calix[4]arene derivative modified on gold nanoparticles and a screen-printed carbon electrode (TC4/AuNPs/SPCE) for Pb2+ and Cu2+ determination. The surface of the modified electrode was characterised via Fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was used for the detection of Pb2+ and Cu2+ under optimum conditions. The limit of detection (LOD) for detecting Pb2+ and Cu2+ was 0.7982 × 10−2 ppm and 1.3358 × 10−2 ppm, respectively. Except for Zn2+ and Hg2+, the presence of competitive ions caused little effect on the current response when detecting Pb2+. However, all competitive ions caused a significant drop in the current response when detecting Cu2+, except Ca2+ and Mg2+, suggesting the sensing platform is more selective toward Pb2+ ions rather than copper (Cu2+) ions. The electrochemical sensor demonstrated good reproducibility and excellent stability with a low relative standard deviation (RSD) value in detecting lead and copper ions. Most importantly, the result obtained in the analysis of Pb2+ and Cu2+ had good recovery in river water, demonstrating the applicability of the developed sensor for real samples.

scholarly journals Non-Enzymatic Impedimetric Sensor Based on 3-Aminophenylboronic Acid Functionalized Screen-Printed Carbon Electrode for Highly Sensitive Glucose Detection

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1686 ◽  
Author(s):  
Ricardo Adriano Dorledo de Faria ◽  
Hassan Iden ◽  
Luiz Guilherme Dias Heneine ◽  
Tulio Matencio ◽  
Younès Messaddeq
Keyword(s):  
Boronic Acid ◽  
Glucose Sensor ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Phenylboronic Acid ◽  
Carbon Surface ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Highly Sensitive ◽  
Screen Printed

A highly sensitive glucose sensor was prepared by a one-step method using 3-aminophenyl boronic acid as a unit of recognition and a screen-printed carbon electrode (SPCE) as an electrochemical transducer. Scanning Electron Microscopy confirmed the success of the functionalization of the SPCE due to the presence of clusters of boronic acid distributed on the carbon surface. In agreement with the Electrochemical Impedance Spectroscopy (EIS) tests performed before and after the functionalization, Cyclic Voltammetry results indicated that the electroactivity of the electrode decreased 37.9% owing to the presence of the poly phenylboronic acid on the electrode surface. EIS revealed that the sensor was capable to selectively detect glucose at a broad range of concentrations (limit of detection of 8.53 × 10−9 M), not recognizing fructose and sucrose. The device presented a stable impedimetric response when immediately prepared but suffered the influence of the storage time and some interfering species (dopamine, NaCl and animal serum). The response time at optimized conditions was estimated to be equal to 4.0 ± 0.6 s.

scholarly journals Disposable Carbon Dots Modified Screen Printed Carbon Electrode Electrochemical Sensor Strip for Selective Detection of Ferric Ions

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Shao Chien Tan ◽  
Suk Fun Chin ◽  
Suh Cem Pang
Keyword(s):  
Electrochemical Sensor ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Carbon Nanodots ◽  
Selective Detection ◽  
Carbon Electrode ◽  
Ferric Ions ◽  
Detection Range ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

A disposable electrochemical sensor strip based on carbon nanodots (C-Dots) modified screen printed carbon electrode (SPCE) was fabricated for selective detection of ferric ions (Fe3+) in aqueous solution. C-Dots of mean diameters within the range of 1–7 nm were synthesized electrochemically from spent battery carbon rods. The analytical performance of this electrochemical sensor strip was characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The deposition of C-Dots had enhanced the electron-transfer kinetics and current intensity of the SPCE remarkably by 734% as compared to that of unmodified SPCE. Under optimized conditions, the electrochemical sensor strip exhibited a linear detection range of 0.5 to 25.0 ppm Fe3+ with a limit of detection (LOD) of 0.44±0.04 ppm (at S/N ratio = 3). Validation of results by the electrochemical sensor strip was done by comparing analysis results obtained using an Atomic Absorption Spectrometer (AAS).

scholarly journals Single-Drop Analysis of Epinephrine and Uric Acid on a Screen-Printed Carbon Electrode

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 285
Author(s):  
David Majer ◽  
Matjaž Finšgar
Keyword(s):  
Uric Acid ◽  
Limit Of Detection ◽  
Ordinary Least Squares ◽  
Carbon Electrode ◽  
Single Drop ◽  
Analytical Methodology ◽  
Screen Printed Carbon Electrode ◽  
Relative Standard ◽  
Weighted Model ◽  
Screen Printed

This work demonstrates the analysis of epinephrine (EP) and uric acid (UA) in a single drop (the volume of the test solution was only 50 µL) using a screen-printed carbon electrode (SPCE) sensor and square-wave voltammetry (SWV). The limit of detection, limit of quantification, linearity, accuracy, precision, and robustness were validated. The normality of the experimental data was tested and confirmed for both methods. Heteroscedasticity was checked by residual analysis followed by a statistical F-test. The latter was confirmed for both analytes. The low relative standard deviations (RSD) at all calibration points and repetitive slopes justified the use of a calibration curve; therefore, the standard addition methodology was avoided (the latter is common in electroanalysis, but time-consuming). Since the conditions for using an ordinary least squares (OLS) regression were not met, weighted linear regression (WLR) was used to improve the accuracy of the analytical results at low concentrations of the analytes. In this manner, the best weighted model was determined and used for the quantification. A comparison was made between the OLS and WLR methods to show the necessity of using the WLR method for EP and UA analysis. The newly developed and validated methods were also shown to be effective in the analysis of real samples. The content of EP in an EP auto-injector and UA in human urine was tested by employing the best weighted model. For EP and UA, the accuracy in terms of the average recovery value was 101.01% and 94.35%, and precision in terms of RSD was 5.65% and 2.75%, respectively. A new analytical methodology is presented that uses a low volume (a single drop), and it offers the advantage of electroanalysis for on-site analysis, where conventional chromatographic techniques cannot be easily employed. Furthermore, the developed technique has additional advantages in terms of speed, cost, and miniaturization.

scholarly journals Development of Highly Sensitive Immunosensor for Clenbuterol Detection by Using Poly(3,4-ethylenedioxythiophene)/Graphene Oxide Modified Screen-Printed Carbon Electrode

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4324 ◽  
Author(s):  
Nurul Talib ◽  
Faridah Salam ◽  
Yusran Sulaiman
Keyword(s):  
Graphene Oxide ◽  
Limit Of Detection ◽  
Food Product ◽  
Electrochemical Immunosensor ◽  
Growth Promoter ◽  
Carbon Electrode ◽  
Electrochemical Assay ◽  
Sensor Platform ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Clenbuterol (CLB) is an antibiotic and illegal growth promoter drug that has a long half-life and easily remains as residue and contaminates the animal-based food product that leads to various health problems. In this work, electrochemical immunosensor based on poly(3,4-ethylenedioxythiophene)/graphene oxide (PEDOT/GO) modified screen-printed carbon electrode (SPCE) for CLB detection was developed for antibiotic monitoring in a food product. The modification of SPCE with PEDOT/GO as a sensor platform was performed through electropolymerization, while the electrochemical assay was accomplished while using direct competitive format in which the free CLB and clenbuterol-horseradish peroxidase (CLB-HRP) in the solution will compete to form binding with the polyclonal anti-clenbuterol antibody (Ab) immobilized onto the modified electrode surface. A linear standard CLB calibration curve with R2 = 0.9619 and low limit of detection (0.196 ng mL−1) was reported. Analysis of milk samples indicated that this immunosensor was able to detect CLB in real samples and the results that were obtained were comparable with enzyme-linked immunosorbent assays (ELISA).

scholarly journals Laccase Electrochemical Biosensor Based on Graphene-Gold/Chitosan Nanocomposite Film for Bisphenol A Detection

2020 ◽  
Vol 16 (5) ◽  
pp. 570-579
Author(s):  
Fuzi M. Fartas ◽  
Jaafar Abdullah ◽  
Nor A. Yusof ◽  
Yusran Sulaiman ◽  
Mohd I. Saiman ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Electrochemical Biosensor ◽  
Limit Of Detection ◽  
Modified Electrodes ◽  
Electrochemical Performances ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Laccase Enzyme ◽  
Bisphenol A Detection ◽  
Screen Printed

Background: Bisphenol A (BPA) is considered one of the most common chemicals that could cause environmental endocrine disrupting. Therefore, there is an increasing demand for simple, rapid and sensitive methods for BPA detection that result from BPA leaching into foods and beverages from storage containers. Herein, a simple laccase electrochemical biosensor was developed for the determination of BPA based on Screen-Printed Carbon Electrode (SPCE) modified graphenegold/ chitosan. The synergic effect of graphene-gold/chitosan nanocomposite as electrode modifier greatly facilitates electron-transfer processes between the electrolyte and laccase enzyme, thus leads to a remarkably improved sensitivity for bisphenol A detection. Methods: In this study, laccase enzyme is immobilized onto the Screen-Printed Carbon Electrode (SPCE) modified Graphene-Decorated Gold Nanoparticles (Gr-AuNPs) with Chitosan (Chit). The surface structure of nanocomposite was studied using different techniques including Field Emission Scanning Microscopy (FESEM), TRANSMISSION Electron Microscopy (TEM), Raman spectroscopy and Energy Dispersive X-ray (EDX). Meanwhile, the electrochemical performances of the modified electrodes were studied using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). Results: The developed laccase biosensor offered excellent analytical performance for the detection of BPA with a sensitivity of 0.271 μA/μM and Limit of Detection (LOD) of 0.023 μM, respectively. Moreover, the constructed biosensor showed good reproducibility, selectivity and stability towards BPA. The sensor has been used to detect BPA in a different type of commercial plastic products as a real sample and satisfactory result was obtained when compared with the HPLC method. Conclusion: The proposed electrochemical laccase biosensor exhibits good result which is considered as a promising candidate for a simple, rapid and sensitive method especially in the resource- limited condition.

Electrochemical Immunosensor Based on Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode

2020 ◽  
Vol 833 ◽  
pp. 171-175
Author(s):  
Nurul Azurin Badruzaman ◽  
Mohd Azraie Mohd Azmi ◽  
Nur Azura Mohd Said
Keyword(s):  
Incubation Time ◽  
Electrochemical Impedance ◽  
Electrochemical Immunosensor ◽  
Graphene Nanoplatelets ◽  
Carbon Electrode ◽  
Functionalized Graphene ◽  
Working Electrode ◽  
Screen Printed Carbon Electrode ◽  
Rabbit Igg ◽  
Screen Printed

We presented here the development of an immunosensor based on graphene nanoplatelets-modified screen printed carbon electrode (SPCE) with incorporated rabbit IgG on the amino functionalized surface area. In order to improve sensitivity of working electrode, graphene-nanoplatelets solution was fabricated onto surface carbon working electrode. The effect of different (3-aminopropyl) triethoxysilane (APTES) concentrations (0.125, 0.5, 2 and 8% (v/v)) and incubation time of silanization (30, 60 and 90 min) were studied and compared. An electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize our immunosensor based. It is showed that the optimum APTES concentration which provides higher surface coverage and electron transfer rate was 2% concentration (v/v) at 60 min of incubation time. The modified surface was then evaluated by measuring immobilized rabbit IgG via indirect assay using horseradish peroxidase labelled secondary antibody. The optimum detection immobilized IgG was 0.05 mg/mL. These results indicate the potential for amino functionalized graphene nanoplatelets-modified SPCE in detecting protein biomarkers.

scholarly journals Uric Acid Biosensor Based on Biofilm of L. plantarum using Screen-Printed Carbon Electrode Modified by Magnetite

Al-Kimia ◽  
2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Dian Siska Rahma Fatonah ◽  
Deden Saprudin ◽  
Dyah Iswantini ◽  
Novik Nurhidayat
Keyword(s):  
Uric Acid ◽  
Limit Of Detection ◽  
Clinical Analysis ◽  
Carbon Electrode ◽  
Range Limit ◽  
Good Electrocatalytic Activity ◽  
Screen Printed Carbon Electrode ◽  
Limit Of Quantitation ◽  
The Stability ◽  
Screen Printed

Biosensor based on biofilm of L. plantarum has been successfully done for determination of uric acid in human urine compared with colorimetric enzymatic produced relative error of less than 5%. L. plantarum has uricase activity to react with uric acid, to maintain the stability of bacteria forming themselves into biofilms. Magnetite is known to increase sensitivity of the biosensor. The combination of magnetite-polyethylene glycol (Fe3O4-PEG) was used to modify the surface of Screen-Printed Carbon Electrode modified (SPCE) and the resulting modified electrode (biofilm/Fe3O4/PEG/SPCE) displayed good electrocatalytic activity to the oxidation of UA. The composition of biofilms with optical density 1, magnetite 100 mgmL-1 and PEG 3% v / v were able to increase the current up to 48% in 4mM of UA. The biosensor with an optimum composition produced good linearity with a concentration range, limit of detection, limit of quantitation, sensitivity, and repeatability were found to be 0.1 - 4.3 mM, 70 µM,  234 µM, 25.392 µA mM-1, 2.38%, respectively. This biosensor stable up to 49 days of measurement with the remaining activity was 90.70% and selective for interference compounds such as salt, urea, glucose, ascorbic acid. This method has a good stability, sensitivity, and potential application in clinical analysis. Keyword: biofilm, biosensor, L. plantarum, magnetite, uric acid.

Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique

2021 ◽  
Vol 24 (2) ◽  
pp. 43-50
Author(s):  
Wulan Tri Wahyuni ◽  
Rudi Heryanto ◽  
Eti Rohaeti ◽  
Achmad Fauzi ◽  
Budi Riza Putra
Keyword(s):  
Uric Acid ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
Electrochemical Measurement ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Screen Printed Carbon Electrode ◽  
Limit Of Quantitation ◽  
Screen Printed

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.

scholarly journals A Disposable Copper (II) Ion Biosensor Based on Self-Assembly of L-Cysteine on Gold Nanoparticle-Modified Screen-Printed Carbon Electrode

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Wong Pooi See ◽  
Sheila Nathan ◽  
Lee Yook Heng
Keyword(s):  
Gold Nanoparticle ◽  
Self Assembly ◽  
Copper Ions ◽  
Effective Means ◽  
Copper Ion ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Preconcentration Time ◽  
Screen Printed

A disposable copper (II) ion biosensor based on self-assembly of L-cysteine on gold nanoparticle-modified screen-printed carbon electrode was fabricated. The electrode was modified by attaching gold nanoparticles onto the surface of screen-printed carbon electrode through seed mediated growth method followed by self-assembly of L-cysteine. As demonstrated by differential pulse voltammetry, the sensor exhibited high sensitivity to copper (II) ion down to ppb (parts per billion) levels. Optimization of various experimental parameters such as pH, buffer concentration, and preconcentration time, which influenced the performance of the biosensor, was investigated. The sensor demonstrated a wide linear response range from 10 to 0.005 ppm(r=0.9870), with a lower detection limit of 8 ppb using 10 min of preconcentration time. The sensor based on screen-printed electrode provides a cost-effective means of application of copper ion sensor for the detection of ppb level of copper ions in water.

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