scholarly journals A Disposable Copper (II) Ion Biosensor Based on Self-Assembly of L-Cysteine on Gold Nanoparticle-Modified Screen-Printed Carbon Electrode

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Wong Pooi See ◽  
Sheila Nathan ◽  
Lee Yook Heng
Keyword(s):  
Gold Nanoparticle ◽  
Self Assembly ◽  
Copper Ions ◽  
Effective Means ◽  
Copper Ion ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Preconcentration Time ◽  
Screen Printed

A disposable copper (II) ion biosensor based on self-assembly of L-cysteine on gold nanoparticle-modified screen-printed carbon electrode was fabricated. The electrode was modified by attaching gold nanoparticles onto the surface of screen-printed carbon electrode through seed mediated growth method followed by self-assembly of L-cysteine. As demonstrated by differential pulse voltammetry, the sensor exhibited high sensitivity to copper (II) ion down to ppb (parts per billion) levels. Optimization of various experimental parameters such as pH, buffer concentration, and preconcentration time, which influenced the performance of the biosensor, was investigated. The sensor demonstrated a wide linear response range from 10 to 0.005 ppm(r=0.9870), with a lower detection limit of 8 ppb using 10 min of preconcentration time. The sensor based on screen-printed electrode provides a cost-effective means of application of copper ion sensor for the detection of ppb level of copper ions in water.

scholarly journals DNA Electrochemical Biosensor Based on Iron Oxide/Nanocellulose Crystalline Composite Modified Screen-Printed Carbon Electrode for Detection of Mycobacterium tuberculosis

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3373
Author(s):  
Mohd Hazani Mat Zaid ◽  
Che Engku Noramalina Che-Engku-Chik ◽  
Nor Azah Yusof ◽  
Jaafar Abdullah ◽  
Siti Sarah Othman ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Iron Oxide ◽  
Differential Pulse ◽  
Ammonium Bromide ◽  
Coupling Mechanism ◽  
Carbon Electrode ◽  
Specific Sequence ◽  
Detection Range ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Death from tuberculosis has resulted in an increased need for early detection to prevent a tuberculosis (TB) epidemic, especially in closed and crowded populations. Herein, a sensitive electrochemical DNA biosensor based on functionalized iron oxide with mercaptopropionic acid (MPA-Fe3O4) nanoparticle and nanocellulose crystalline functionalized cetyl trimethyl ammonium bromide (NCC/CTAB) has been fabricated for the detection of Mycobacterium tuberculosis (MTB). In this study, a simple drop cast method was applied to deposit solution of MPA-Fe3O4/NCC/CTAB onto the surface of the screen-printed carbon electrode (SPCE). Then, a specific sequence of MTB DNA probe was immobilized onto a modified SPCE surface by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling mechanism. For better signal amplification and electrochemical response, ruthenium bipyridyl Ru(bpy)32+ was assigned as labels of hybridization followed by the characteristic test using differential pulse voltammetry (DPV). The results of this biosensor enable the detection of target DNA until a concentration as low as 7.96 × 10−13 M with a wide detection range from 1.0 × 10−6 to 1.0 × 10−12 M. In addition, the developed biosensor has shown a differentiation between positive and negative MTB samples in real sampel analysis.

scholarly journals The Scavenging Effect of Myoglobin from Meat Extracts toward Peroxynitrite Studied with a Flow Injection System Based on Electrochemical Reduction over a Screen-Printed Carbon Electrode Modified with Cobalt Phthalocyanine: Quantification and Kinetics

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 220
Author(s):  
Ioana Silvia Hosu ◽  
Diana Constantinescu-Aruxandei ◽  
Florin Oancea ◽  
Mihaela Doni
Keyword(s):  
Flow Injection ◽  
Linear Sweep Voltammetry ◽  
Differential Pulse ◽  
Injection System ◽  
Cobalt Phthalocyanine ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

The scavenging activity of myoglobin toward peroxynitrite (PON) was studied in meat extracts, using a new developed electrochemical method (based on cobalt phthalocyanine-modified screen-printed carbon electrode, SPCE/CoPc) and calculating kinetic parameters of PON decay (such as half-time and apparent rate constants). As reactive oxygen/nitrogen species (ROS/RNS) affect the food quality, the consumers can be negatively influenced. The discoloration, rancidity, and flavor of meat are altered in the presence of these species, such as PON. Our new highly thermically stable, cost-effective, rapid, and simple electrocatalytical method was combined with a flow injection analysis system to achieve high sensitivity (10.843 nA µM−1) at a nanomolar level LoD (400 nM), within a linear range of 3–180 µM. The proposed biosensor was fully characterized using SEM, FTIR, Raman spectroscopy, Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV), and Linear Sweep Voltammetry (LSV). These achievements were obtained due to the CoPc-mediated reduction of PON at very low potentials (around 0.1 V vs. Ag/AgCl pseudoreference). We also proposed a redox mechanism involving two electrons in the reduction of peroxynitrite to nitrite and studied some important interfering species (nitrite, nitrate, hydrogen peroxide, dopamine, ascorbic acid), which showed that our method is highly selective. These features make our work relevant, as it could be further applied to study the kinetics of important oxidative processes in vivo or in vitro, as PON is usually present in the nanomolar or micromolar range in physiological conditions, and our method is sensitive enough to be applied.

scholarly journals 3A-Amino-3A-Deoxy-(2AS, 3AS)-β-Cyclodextrin Hydrate/Tin Disulfide Modified Screen-Printed Carbon Electrode for the Electrochemical Detection of Polychlorinated Biphenyls

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Xinke Liu ◽  
Rajalakshmi Sakthivel ◽  
Chia-Yin Cheng ◽  
Jiangliu Luo ◽  
Lijun Song ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Detection Range ◽  
Tin Disulfide ◽  
Screen Printed Carbon Electrode ◽  
Endocrine Disrupting ◽  
Pulse Voltammetry ◽  
Limit Detection ◽  
Screen Printed

AbstractPolychlorinated biphenyls (PCBs) are persistent organic pollutants that are widely distributed in the environment. It is noteworthy that the PCBs are endocrine-disrupting substances, and their toxicity induces cancer and damage to the mammalian reproductive system, immune system, stomach, skin, liver, etc. This work aimed to synthesize 3A-amino-3A-deoxy-(2AS, 3AS)-β-cyclodextrin hydrate/tin disulfide composite material and to study its material properties, electrochemical properties, and application to PCB detection. The nanostructured tin disulfide (SnS2) synthesized by hydrothermal technique and 3A-amino-3A-deoxy-(2AS, 3AS)-β-cyclodextrin hydrate were sequentially modified onto the disposable screen-printed carbon electrode (SPCE) via titration using a micropipette. The 3A-amino-3A-deoxy-(2AS, 3AS)-β-cyclodextrin hydrate (β-CD) improved the selectivity of the modified electrode. The fabricated β-CD/SnS2/SPCE was employed to determine the presence of PCBs by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The detection range was 0.625–80 μM, with a limit detection of approximately 5 μM. The electrodes were as stable as 88% after 7 days’ storage. The results showed that the β-CD successfully encapsulated PCBs to achieve an electrochemical sensor that reduced the time and increased the convenience of PCBs detection.

Uric Acid Sensor Based on PEDOT:PSS Modified Screen-Printed Carbon Electrode Fabricated with a Simple Painting Technique

2021 ◽  
Vol 24 (2) ◽  
pp. 43-50
Author(s):  
Wulan Tri Wahyuni ◽  
Rudi Heryanto ◽  
Eti Rohaeti ◽  
Achmad Fauzi ◽  
Budi Riza Putra
Keyword(s):  
Uric Acid ◽  
Electrochemical Sensors ◽  
Limit Of Detection ◽  
Electrochemical Measurement ◽  
Differential Pulse ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Screen Printed Carbon Electrode ◽  
Limit Of Quantitation ◽  
Screen Printed

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.

Self-Assembly Films on a Screen-Printed Carbon Electrode

2013 ◽  
Vol 341-342 ◽  
pp. 307-310
Author(s):  
Yu Fang Sha ◽  
Hong Jun Lan ◽  
Yan Zhang ◽  
Shou Shuo Liu ◽  
Jun Xie
Keyword(s):  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Multiwall Carbon Nanotubes ◽  
Multilayer Films ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Redox Polymer ◽  
Screen Printed Carbon Electrode ◽  
Multiwall Carbon ◽  
Screen Printed

Using layer-by-layer (LBL) assembled method, multilayer films containing multiwall carbon nanotubes (MWNTs) and redox polymer were successfully fabricated on a screen-printed carbon electrode. X-ray photoelectron spectroscopy (XPS) and field-emission scanning electron microscopy (FE-SEM) were used to characterize the assembled multilayer films.

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