Phytochemicals and elucidation of polyphenol compounds of aqueous-methanol fraction of <em>Desmodium</em> <em>velutinum</em> stem using high performance liquid chromatography

A rapid and sensitive method is described for the extraction, detection and measurement of corynetoxins in ryegrass seed galls colonized by Corynebucterium rathayi. Excised galls are extracted with aqueous methanol and the extract analysed, without further purification, by reversed-phase high-performance liquid chromatography with detection by uItraviolet absorption. The method is applicable down to the level of a single gall or part of a gall with a minimum level of detection of about 0.25 �g using the equipment described. The corynetoxin content of the individual galls examined varied from below the detection limit to 10.6 �g. The highest level of corynetoxin in a single gall was 1.15% of gall weight. The corynetoxin composition of galls collected during outbreaks of annual ryegrass toxicity in South Australia, Western Australia and South Africa during a 19-year period were found to be similar.

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The Presence of 5,7-Dihydroxyisoflavone in Peanuts by High Performance Liquid Chromatography Analyses

Peanut Science ◽

10.3146/pnut.12.2.0003 ◽

1985 ◽

Vol 12 (2) ◽

pp. 60-62 ◽

Cited By ~ 6

Author(s):

D. J Daigle ◽

R. L Ory ◽

W. D Branch

Keyword(s):

High Pressure ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Arachis Hypogaea ◽

High Performance ◽

Aqueous Methanol ◽

Uv Spectrum ◽

Tentative Identification ◽

Arachis Hypogaea L ◽

Flavonoid Aglycone

Abstract The defatted flours of 43 colored and one white testa peanut (Arachis hypogaea L.) genotypes were analyzed for flavonoids. Flavonoids were extracted from the flours with aqueous methanol, hydrolyzed, and the resulting aglycones analyzed by high pressure liquid chromatography with a variable wavelenth detector. The UV spectrum and retention time coupled with those of standards allowed for the tentative identification of the principal flavonoid aglycone as 5,7-dihydroxyisoflavone (DHI).

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Changes of Polyphenol Compound Concentrations in Hybrids of Nante Type Carrots during Storage

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.1515/prolas-2017-0085 ◽

2017 ◽

Vol 71 (6) ◽

pp. 492-495 ◽

Cited By ~ 1

Author(s):

Ingrīda Augšpole ◽

Tatjana Kince ◽

Ingmārs Cinkmanis

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Ferulic Acid ◽

Chlorogenic Acid ◽

Gallic Acid ◽

Caffeic Acid ◽

High Performance ◽

Polyphenol Compounds ◽

Polyphenol Compound

Abstract The main purpose of the study was to determine changes of polyphenol concentrations in hybrids of Nante type carrots during storage. Fresh Nante type ‘Forto’ variety carrots and carrot hybrids ‘Bolero’ F1, ‘Champion’ F1, and ‘Maestro’ F1 were cultivated in the Zemgale region of Latvia. Carrots were stored for six months in air (+3 ± 1 °C, RH = 89 ± 1%) and polyphenol compound concentrations were determined at two month intervals. High-performance liquid chromatography was used to determine concentrations of eight polyphenols in carrots: gallic acid, catechin, epicatechin, caffeic acid, chlorogenic acid, ferulic acid, vanillin, and rutin. Significant differences occurred in polyphenol concentrations of fresh Nante type variety ‘Forto’ carrots and several hybrids (‘Bolero’ F1, ‘Champion’ F1, and ‘Maestro’ F1) during storage. After six months of storage, the concentration of polyphenol compounds of Nante type carrots decreased — caffeic acid by 64.6%, chlorogenic acid — by 37.9% and vanillin — by 81.5%. However, during storage, concentration of some polyphenol compounds increased, as catechin by 30.5%, epicatechin by 85.2%, gallic acid by 48.5% and ferulic acid by 87.9%.

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