scholarly journals Potential Protein and Biodiesel Sources from Black Soldier Fly Larvae: Insights of Larval Harvesting Instar and Fermented Feeding Medium

Energies ◽  
2019 ◽  
Vol 12 (8) ◽  
pp. 1570 ◽  
Author(s):  
Chung-Yiin Wong ◽  
Siti-Suhailah Rosli ◽  
Yoshimitsu Uemura ◽  
Yeek Chia Ho ◽  
Arunsri Leejeerajumnean ◽  
...  

Primarily produced via transesterification of lipid sources, fatty acid methyl ester (FAME) of biodiesel derived from insect larvae has gained momentum in a great deal of research done over other types of feedstock. From the self-harvesting nature of black soldier fly larvae (BSFL), research had, however, only concentrated on the harvest of BSFL on sixth instar. Through rearing BSFL on coconut endosperm waste (CEW), 100 BSFL were harvested at the fifth and sixth instar, then modification on CEW with mixed-bacteria powder was carried out. It was found that the fifth instar BSFL had 34% lipid content, which was 8% more than the sixth instar. Both instars had similar corrected protein contents around 35–38%. The sixth instar BSFL contained around 19% of chitin, which was about 11% more than the fifth instar. Biodiesel products from both instars showed no differences in terms of FAME content. With modification on CEW, at 0.5 wt% of mixed-bacteria powder concentration, the maximum waste-to-biomass conversion (WBC) and protein conversion (PC) were achieved at 9% and 60%, respectively. Moreover, even with the shorter fermentation time frame of CEW, it did not affect the development of BSFL in terms of its WBC and PC when fed with 14 and 21 days fermented medium. FAME from all groups set, which predominantly constituted about C12:0 at around 60%, followed by C14:0 at around 15%, C16:0, and C18:1 both at 10% on average. Lastly, the FAME yield from BSFL was improved from 25% (sixth instar) to 33% (fifth instar) and showed its highest at 38.5% with modification on raw CEW with 0.5 wt% mixed-bacteria powder and fermented for 21 days. Thus, harvesting BSFL at earlier instar is more beneficial and practical, as it improves the FAME yield from the BSFL biomass.

2018 ◽  
Vol 25 (2) ◽  
pp. 79
Author(s):  
Ida Kinasih ◽  
Ramadhani Eka Putra ◽  
Agus Dana Permana ◽  
Finsa Firlana Gusmara ◽  
Muhammad Yusuf Nurhadi ◽  
...  

Insect larvae, such as black soldier fly larvae (BSFL: Hermetia illucens) are currently being considered as an alternative source of protein for poultry and aquaculture industry while in the same time apply as agent to manage organic wastes. In order to achieve both roles it is necessary to develop an appropriate farming methodology and understand the impact of different feeding source for the insect production. The study explored the effect of three local plant based organic wastes as feedstocks to growth of the black soldier fly larvae. Separate feeding trials on horse manure (HM), vegetable wastes (VW), and tofu dreg (TD) was done and their effect on BSF larvae growth rate, larvae weight and total prepupal yield in grams content analysed. The efficiency of the BSF larvae to consume and ability to reduce the waste load of the different substrates was also evaluated. The result showed all materials suitable as feeding material for black soldier fly. Difference on physical properties and chemical content affected the development time, harvested biomass, efficiency of digestivity, and efficiency of biomass production which is unique for each type of waste. This study provide base line information for future development of plant based organic waste management.


Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 299
Author(s):  
Chung Yiin Wong ◽  
Kunlanan Kiatkittipong ◽  
Worapon Kiatkittipong ◽  
Jun Wei Lim ◽  
Man Kee Lam ◽  
...  

Coconut endosperm waste (CEW) was treated by Rhizopus oligosporus via in situ and ex situ fermentations together with bioconversion into valuable black soldier fly larval biomass. The ex situ fermentation could overall enrich the nutritional compositions of CEW by hydrolyzing its complex organic polymers and exuding assimilable nutrients to enhance the black soldier fly larvae (BSFL) growth. Nevertheless, the larval gut bacteria were competing with Rhizopus oligosporus in in situ fermentation, derailing the hydrolysis processes and larval growth. Accordingly, the highest growth rates achieved were around 0.095 g/day, as opposed to only 0.065 g/day whilst using 0.5 wt% of Rhizopus oligosporus to perform ex situ and in situ fermentations, respectively. These were also underpinned by the greater amount of total CEW consumed when employing ex situ fermentation, with comparable metabolic costs to feeding on in situ-fermented CEW. The mature BSFL were subsequently harvested and the amounts of protein and lipid produced were assessed in terms of their feasibility for biodiesel production. While the statistical analyses showed that the larval protein yields derived from both fermentation modes were insignificant, the BSFL could attain higher lipid and protein productivities upon feeding with ex situ- rather than in situ-fermented CEW mediums. Better yet, the larval biodiesel quality measured in terms of the fatty acid methyl ester composition were not varied significantly by Rhizopus oligosporus through the fermentation process. Thereby, the presence of 1.0 wt% Rhizopus oligosporus was considered optimum to perform ex situ fermentation, giving rise to the acceptable growth of BSFL loaded with the highest lipid yield and productivity for producing biodiesel and protein simultaneously.


animal ◽  
2018 ◽  
Vol 12 (8) ◽  
pp. 1672-1681 ◽  
Author(s):  
S. Mancini ◽  
I. Medina ◽  
V. Iaconisi ◽  
F. Gai ◽  
A. Basto ◽  
...  

2021 ◽  
pp. 101034
Author(s):  
M. Heuel ◽  
C. Sandrock ◽  
F. Leiber ◽  
A. Mathys ◽  
M. Gold ◽  
...  

2021 ◽  
pp. 101400
Author(s):  
Fernanda M. Tahamtani ◽  
Emma Ivarsson ◽  
Viktoria Wiklicky ◽  
Cecilia Lalander ◽  
Helena Wall ◽  
...  

2021 ◽  
Vol 13 (11) ◽  
pp. 6074
Author(s):  
Esther Khayanga Sumbule ◽  
Mary Kivali Ambula ◽  
Isaac Maina Osuga ◽  
Janice Ghemoh Changeh ◽  
David Miano Mwangi ◽  
...  

The acceptance of eco-friendly black soldier fly larvae meal (BSFLM) as sustainable alternative protein ingredient in poultry feeds continues to gain momentum worldwide. This study evaluates the impact of BSFLM in layer chick and grower diets on the growth, carcass quality and economic returns. Mean weekly weight gain and total live weight per chick and grower varied significantly. The highest final weight gain was achieved when birds were provided diet with 25.6% BSFLM. Average daily feed intake (ADFI), average daily weight gain (ADG) and overall weight gain of the chick varied significantly, except for the feed conversion ratio (FCR). For grower birds, ADFI, ADG, FCR and overall weight gain did not vary significantly across the various feeding regimes. The weight of the wings and drumsticks had a quadratic response with a maximum weight obtained at 33% inclusion of BSFLM. The weight of the internal organs were not significantly affected by dietary types. Positive cost–benefit ratio and return on investment was recorded for diet types with higher BSFLM inclusion levels (>75%). Diets with 25% and 100% BSFLM inclusion were the most suitable and cost-effective, respectively. Thus, BSFLM represents a promising alternative source of protein that could be sustainably used in the poultry industries.


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