scholarly journals Low Water Availability Increases Necrosis in Picea abies after Artificial Inoculation with Fungal Root Rot Pathogens Heterobasidion parviporum and Heterobasidion annosum

Forests ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 55 ◽  
Author(s):  
Eeva Terhonen ◽  
Gitta Langer ◽  
Johanna Bußkamp ◽  
David Rӑscuţoi ◽  
Kathrin Blumenstein
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Water Availability ◽  
High Water ◽  
Climatic Conditions ◽  
Heterobasidion Annosum ◽  
Economic Losses ◽  
Negative Effects ◽  
Water Group ◽  
Horizontal Length

Research Highlights: Dedicated experiments to investigate how disturbances will affect Heterobasidion sp.—Norway spruce pathosystems are important, in order to develop different strategies to limit the spread of Heterobasidion annosum s.l. under the predicted climate change. Here, we report on a greenhouse experiment to evaluate the effects of water availability on the infection severity of Heterobasidion parviporum or Heterobasidion annosum, respectively, on Picea abies saplings. Background and Objectives: Changes in climatic conditions and intense logging will continue to promote H. annosum s.l. in conifer forests, increasing annual economic losses. Thus, our aim was to test if disease severity in Norway spruce was greater after infection with H. parviporum or H. annosum in low water availability conditions, compared to seedlings with high water availability. Materials and Methods: We performed inoculation studies of three-year-old saplings in a greenhouse. Saplings were treated as high (+) or low (−) water groups: High water group received double the water amount than the low water group. The necrosis observed after pathogen inoculation was measured and analyzed. Results: The seedling growth was negatively influenced in the lower water group. In addition, the water availability enhanced the necrosis length of H. parviporum in phloem and sapwood (vertical length) in the low water group. H. annosum benefited only in horizontal length in the phloem. Conclusions: Disturbances related to water availability, especially low water conditions, can have negative effects on the tree host and benefit the infection ability of the pathogens in the host.

scholarly journals Drought Stress Described by Transcriptional Responses of Picea Abies under Pathogen Heterobasidion Parviporum Attack

2021 ◽  
Author(s):  
Xenia Hao-Yi Yeoh ◽  
Blessing Durodola ◽  
Kathrin Blumenstein ◽  
Eeva Terhonen
Keyword(s):  
Climate Change ◽  
Drought Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Water Availability ◽  
Differentially Expressed Gene ◽  
Differentially Expressed ◽  
Gene Expressions ◽  
Transcriptional Responses ◽  
Water Group

The major threats to the sustainable supply of forest tree products are adverse climate, pests and diseases. Climate change, exemplified by increased drought, poses a unique threat to global forest health. This is attributed to the unpredictable behavior of forest pathosystems, which can favor fungal pathogens over the host under persistent drought stress conditions in the future. Currently, the effects of drought on tree resistance against pathogens are hypothetical, thus research is needed to identify these correlations. Norway spruce (Picea abies) is one of the most economically important tree species in Europe, and is considered highly vulnerable to changes in climate. Dedicated experiments to investigate how disturbances will affect the Norway spruce - Heterobasidion sp. pathosystem are important, in order to develop different strategies to limit the spread of H. annosum s.l. under the predicted climate change. Here, we report a transcriptional study to compare Norway spruce gene expressions to evaluate the effects of water availability and the infection of Heterobasidion parviporum. We performed inoculation studies of three-year-old saplings in a greenhouse (purchased from a nursery). Norway spruce saplings were treated in either high (+) or low (-) water groups: high water group received double the water amount than the low water group. RNA was extracted and sequenced. Similarly, we quantified gene expression levels of candidate genes in biotic stress and jasmonic acid (JA) signaling pathways using qRT-PCR, through which we discovered a unique preferential defense response of H. parviporum-infected Norway spruce under drought stress at the molecular level. Disturbances related to water availability, especially low water conditions can have negative effects on the tree host and benefit the infection ability of the pathogens in the host. From our RNA-seq analysis, 114 differentially expressed gene regions were identified between high (+) and low (-) water groups under pathogen attack. None of these gene pathways were identified to be differentially expressed from both non-treated and mock-control treatments between high (+) and low (-) water groups. Finally, only four genes were found to be associated with drought in all treatments.

scholarly journals Drought Stress Described by Transcriptional Responses of Picea Abies (L.) H. Karst. under Pathogen Heterobasidion Parviporum Attack

Forests ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1379
Author(s):  
Xenia Hao-Yi Yeoh ◽  
Blessing Durodola ◽  
Kathrin Blumenstein ◽  
Eeva Terhonen
Keyword(s):  
Climate Change ◽  
Drought Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Water Availability ◽  
Differentially Expressed Gene ◽  
Differentially Expressed ◽  
Gene Expressions ◽  
Transcriptional Responses ◽  
Water Group

The major threats to the sustainable supply of forest tree products are adverse climate, pests and diseases. Climate change, exemplified by increased drought, poses a unique threat to global forest health. This is attributed to the unpredictable behavior of forest pathosystems, which can favor fungal pathogens over the host under persistent drought stress conditions in the future. Currently, the effects of drought on tree resistance against pathogens are hypothetical, thus research is needed to identify these correlations. Norway spruce (Picea abies (L.) H. Karst.) is one of the most economically important tree species in Europe and is considered highly vulnerable to changes in climate. Dedicated experiments to investigate how disturbances will affect the Norway spruce—Heterobasidion sp. pathosystem are important, in order to develop different strategies to limit the spread of H. annosum s.l. under the predicted climate change. Here, we report a transcriptional study to compare Norway spruce gene expressions to evaluate the effects of water availability and the infection of Heterobasidion parviporum. We performed inoculation studies of three-year-old saplings in a greenhouse (purchased from a nursery). Norway spruce saplings were treated in either high (+) or low (−) water groups: high water group received double the water amount than the low water group. RNA was extracted and sequenced. Similarly, we quantified gene expression levels of candidate genes in biotic stress and jasmonic acid (JA) signaling pathways using qRT-PCR, through which we discovered a unique preferential defense response of H. parviporum-infected Norway spruce under drought stress at the molecular level. Disturbances related to water availability, especially low water conditions can have negative effects on the tree host and benefit the infection ability of the pathogens in the host. From our RNA-seq analysis, 114 differentially expressed gene regions were identified between high (+) and low (−) water groups under pathogen attack. None of these gene pathways were identified to be differentially expressed from both non-treated and mock-control treatments between high (+) and low (−) water groups. Finally, only four genes were found to be associated with drought in all treatments.

scholarly journals The Profitability of Cross-Cutting Practices in Butt-Rotten Picea abies Final-Felling Stands

Forests ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 874 ◽  
Author(s):  
Kärhä ◽  
Räsänen ◽  
Palander
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Processing Time ◽  
Wood Quality ◽  
Study Data ◽  
Heterobasidion Annosum ◽  
Time And Motion ◽  
The Cross ◽  
Motion Studies ◽  
The Impact

Research Highlights: This study offers new information on the cross cutting of decayed stems with the sounding of short (0.5 m) offcuts and the bucking of longer (3.0 m) butt-rotten poles. Background and Objectives: The root and butt-rot fungus Heterobasidion annosum sensu lato (Fr.) Bref. causes wood quality damage to trees in softwood forests. When timber is harvested in butt-rotten forests, it is essential that the decayed part of the tree is recognized and cut away from a stem, while the healthy and good quality log section of a stem is cross cut with precision sawlogs. The objective of the study was to investigate the impact of two off-cutting methods on stem processing time, cutting productivity, sawlog volume, and commercial value at the roadside landing when harvesting timber from the butt-rotten Norway spruce (Picea abies (L.) Karst.) final-felling forests. Materials and Methods: The length of the short offcuts used was 0.5 m. The results of the cross-cutting practices were compared to the decayed pulpwood poles of 3 m from the butt of the rotten stems. Time and motion studies were carried out in stands before the profitability calculations. The study data consisted of 1980 Norway spruce sawlog stems. Results: Sounding of the short offcuts added significantly to the stem processing time of butt-rotten stems, but the sawlog volume and the timber value recovery of the stems were higher than those of the decayed pulpwood poles of 3 m. Conclusions: The study concluded that sounding of butt-rotten Norway spruce stems with one to three offcuts is economically profitable if the diameter of the decayed column at the stem stump’s height is small (≤5 cm). In contrast, when the width of the decay is larger (>5 cm), it is more profitable to first cross cut the decayed pulpwood pole of 3 m and then to observe the height of the decayed part of the stem.

Spread of S and P group isolates of Heterobasidion annosum within and among Picea abies trees in central Lithuania

1998 ◽  
Vol 28 (7) ◽  
pp. 961-966 ◽  
Author(s):  
Rimvydas Vasiliauskas ◽  
Jan Stenlid
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Field Data ◽  
Heterobasidion Annosum ◽  
Related Difference ◽  
Spruce Wood

Spread of Heterobasidion annosum (Fr.) Bref. was investigated in a 60-year-old Norway spruce (Picea abies (L.) Karst.) stand in central Lithuania. Of the 375 trees sampled, H. annosum was isolated from 85 (22.7%). Forty nine (56.3%) of the isolates belonged to the S group and 38 (43.7%) to the P group. Among the S-group isolates, 35 genets occupied 49 trees (1.4 trees per genet on average). In the P group, 24 genets were detected in 37 trees (1.5 trees per genet on average). The largest S and P genets were 21 and 20 m in diameter, respectively. The S group spread was more extensive within stems than for the P group; mean length of the decay column for S-group isolates was 459 ± 159 cm (mean ± SD), and it was 327 ± 101 cm for P-group isolates (p = 0.0001). Average stump area affected by decay was 56 ± 15%for the S group, and 47 ± 16% for the P group (p = 0.016). This is the first published field data on intersterility group related difference in decay extension in tree stems. The S group is better adapted than the P group to growth in Norway spruce wood.

scholarly journals Covering Norway spruce container seedlings with reflective shading cloth during field storage affects seedling post-planting growth

New Forests ◽  
2021 ◽  
Author(s):  
Mikko Tikkinen ◽  
Johanna Riikonen ◽  
Jaana Luoranen
Keyword(s):  
Norway Spruce ◽  
Grey Mould ◽  
Storage Conditions ◽  
Climatic Conditions ◽  
Test Field ◽  
Stem Water Potential ◽  
Negative Effects ◽  
Seedling Mortality ◽  
Container Seedlings ◽  
Growing Seasons

AbstractThe field storage of container seedlings is often a necessity after the seedlings have been transported from nurseries close to the regeneration area. Suboptimal storage conditions can lead to reduced growth or even seedling mortality. Supporting favourable conditions for the seedlings during storage requires labour, especially for the watering of seedlings, and therefore increases the costs of forest regeneration. In this study, we examined the effects of covering Norway spruce container seedlings with a reflective shading cloth and neglecting the watering during field storage. This was done by measuring the drying of the seedlings during field storage, the rooting of the seedlings when planting, and the subsequent survival and performance in the field two and three growing seasons after planting. The study comprised one-year-old Norway spruce container seedlings covered with reflective shading cloth and seedlings with no cover (subject to rainfall) in 2012 and 2013; additionally, in 2013, replicates from both treatments were stored in conditions where rainfall was impeded. The stem water potential was measured, a rooting test was carried out, and the seedlings were planted in the test field weekly over 42 days of field storage without watering. As a novel finding, the results indicate that covering the seedlings with a reflective shading cloth during the field storage increased the risk of grey mould in summer storage and led to negative effects regarding post planting seedling development. The application of a shading cloth directly on the seedlings cannot be recommended to prolong the watering intervals during field storage in the current climatic conditions in Southern Finland, where the risk of grey mould is high.

Interactions between Norway spruce (Picea abies) and Heterobasidion annosum. I. Infection of nonsuberized and young suberized roots

1994 ◽  
Vol 72 (6) ◽  
pp. 872-883 ◽  
Author(s):  
W. K. Heneen ◽  
M. Gustafsson ◽  
G. Karlsson ◽  
K. Brismar
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Cell Walls ◽  
Heterobasidion Annosum ◽  
Root Infection ◽  
Host Reaction ◽  
Outer Cortex ◽  
Fungal Enzymes ◽  
Transmission Electron ◽  
Inner Cortex

Nonsuberized and young suberized roots of Norway spruce (Picea abies) were inoculated with an S-strain of Heterobasidion annosum for 2–20 days. The roots were sectioned for light microscopy and transmission electron microscopy. They were susceptible to infection at both stages of development. The host reaction to delimit the infection was the formation of a necrotic ring barrier in the outer cortex. In cases where the inner cortex also became infected, fungal hyphae accumulated just before the endodermis, which acted as a new barrier. Only in nonsuberized roots did the hyphae succeed in penetrating the stele, and within 3 days after inoculation the stele was almost completely digested. Other resistance reactions included accumulation of phenolic deposits, secondary thickening of cell walls, and formation of papillae. The hyphae were able to grow within and across cell walls. The presence of translucent areas around penetrating hyphae possibly reflected the digestive action of fungal enzymes. The hyphae showed signs of degeneration when entrapped in encompassing cellular deposits. Key words: Norway spruce, Heterobasidion annosum, root infection, nonsuberized roots, young suberized roots, microscopy.

scholarly journals Susceptibility of Picea abies and Pinus sylvestris Seedlings of Various origins to Heterobasidion annosum and H. parviporum

2016 ◽  
Vol 70 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Astra Zaļuma ◽  
Arnis Gailis ◽  
Natālija Burņeviča ◽  
Kari Korhonen ◽  
Tālis Gaitnieks
Keyword(s):  
Picea Abies ◽  
Pinus Sylvestris ◽  
Norway Spruce ◽  
Scots Pine ◽  
Fungal Growth ◽  
Seed Source ◽  
Heterobasidion Annosum ◽  
Seed Sources ◽  
Pine Seedlings

Abstract Five-year-old Norway spruce and four-year-old Scots pine seedlings of various origin were inoculated with Heterobasidion annosum s.s. and H. parviporum to estimate whether the susceptibility of seedlings to Heterobasidion was affected by origin of seeds. In total, 520 spruce and 538 pine seedlings from different seed sources and provenance regions of Latvia were tested. Four months after inoculation the fungal growth was measured. The results highlight differences between development of H. annosum and H. parviporum in spruce and pine seedlings. We did not find any seed source that was more resistant than others.

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