Comparison of Engineered Peptide-Glycosaminoglycan Microfibrous Hybrid Scaffolds for Potential Applications in Cartilage Tissue Regeneration

AbstractSkin is the body’s first barrier against external pathogens that maintains the homeostasis of the body. Any serious damage to the skin could have an impact on human health and quality of life. Tissue engineering aims to improve the quality of damaged tissue regeneration. One of the most effective treatments for skin tissue regeneration is to improve angiogenesis during the healing period. Over the last decade, there has been an impressive growth of new potential applications for nanobiomaterials in tissue engineering. Various approaches have been developed to improve the rate and quality of the healing process using angiogenic nanomaterials. In this review, we focused on molecular mechanisms and key factors in angiogenesis, the role of nanobiomaterials in angiogenesis, and scaffold-based tissue engineering approaches for accelerated wound healing based on improved angiogenesis.

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A multiscale approach in the computational modeling of the biophysical environment in artificial cartilage tissue regeneration

Biomechanics and Modeling in Mechanobiology ◽

10.1007/s10237-012-0440-5 ◽

2012 ◽

Vol 12 (4) ◽

pp. 763-780 ◽

Cited By ~ 12

Author(s):

Paola Causin ◽

Riccardo Sacco ◽

Maurizio Verri

Keyword(s):

Computational Modeling ◽

Tissue Regeneration ◽

Cartilage Tissue ◽

Multiscale Approach ◽

Artificial Cartilage ◽

Biophysical Environment

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Development of Multilayered Chlorogenate-Peptide Based Biocomposite Scaffolds for Potential Applications in Ligament Tissue Engineering - An In Vitro Study

Journal of Biomimetics Biomaterials and Biomedical Engineering ◽

10.4028/www.scientific.net/jbbbe.34.37 ◽

2017 ◽

Vol 34 ◽

pp. 37-56 ◽

Cited By ~ 1

Author(s):

Harrison T. Pajovich ◽

Alexandra M. Brown ◽

Andrew M. Smith ◽

Sara K. Hurley ◽

Jessica R. Dorilio ◽

...

Keyword(s):

Tissue Regeneration ◽

Type I Collagen ◽

Phase Changes ◽

Peptide Sequence ◽

Proteoglycan Synthesis ◽

Type I ◽

Average Roughness ◽

Potential Applications ◽

Self Assembled

In this work, for the first time, chlorogenic acid, a natural phytochemical, was conjugated to a lactoferrin derived antimicrobial peptide sequence RRWQWRMKKLG to develop a self-assembled template. To mimic the components of extracellular matrix, we then incorporated Type I Collagen, followed by a sequence of aggrecan peptide (ATEGQVRVNSIYQDKVSL) onto the self-assembled templates for potential applications in ligament tissue regeneration. Mechanical properties and surface roughness were studied and the scaffolds displayed a Young’s Modulus of 169 MP and an average roughness of 72 nm respectively. Thermal phase changes were studied by DSC analysis. Results showed short endothermic peaks due to water loss and an exothermic peak due to crystallization of the scaffold caused by rearrangement of the components. Biodegradability studies indicated a percent weight loss of 27.5 % over a period of 37 days. Furthermore, the scaffolds were found to adhere to fibroblasts, the main cellular component of ligament tissue. The scaffolds promoted cell proliferation and displayed actin stress fibers indicative of cell motility and attachment. Collagen and proteoglycan synthesis were also promoted, demonstrating increased expression and deposition of collagen and proteoglycans. Additionally, the scaffolds exhibited antimicrobial activity against Staphylococcus epidermis bacteria, which is beneficial for minimizing biofilm formation if potentially used as implants. Thus, we have developed a novel biocomposite that may open new avenues to enhance ligament tissue regeneration.

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Biomimetic composites and stem cells interaction for bone and cartilage tissue regeneration

Journal of Materials Chemistry ◽

10.1039/c1jm14401d ◽

2012 ◽

Vol 22 (12) ◽

pp. 5239 ◽

Cited By ~ 29

Author(s):

N. Naveena ◽

J. Venugopal ◽

R. Rajeswari ◽

S. Sundarrajan ◽

R. Sridhar ◽

...

Keyword(s):

Stem Cells ◽

Tissue Regeneration ◽

Cartilage Tissue ◽

And Cartilage

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Tuning the Properties of PNIPAm-Based Hydrogel Scaffolds for Cartilage Tissue Engineering

Polymers ◽

10.3390/polym13183154 ◽

2021 ◽

Vol 13 (18) ◽

pp. 3154

Author(s):

Md Mohosin Rana ◽

Hector De la Hoz Siegler

Keyword(s):

Tissue Engineering ◽

Physical Properties ◽

Tissue Regeneration ◽

Structural Integrity ◽

Cartilage Tissue ◽

Design Parameters ◽

Comprehensive Overview ◽

Hydrogel Scaffolds ◽

Design Variables

Poly(N-isopropylacrylamide) (PNIPAm) is a three-dimensional (3D) crosslinked polymer that can interact with human cells and play an important role in the development of tissue morphogenesis in both in vitro and in vivo conditions. PNIPAm-based scaffolds possess many desirable structural and physical properties required for tissue regeneration, but insufficient mechanical strength, biocompatibility, and biomimicry for tissue development remain obstacles for their application in tissue engineering. The structural integrity and physical properties of the hydrogels depend on the crosslinks formed between polymer chains during synthesis. A variety of design variables including crosslinker content, the combination of natural and synthetic polymers, and solvent type have been explored over the past decade to develop PNIPAm-based scaffolds with optimized properties suitable for tissue engineering applications. These design parameters have been implemented to provide hydrogel scaffolds with dynamic and spatially patterned cues that mimic the biological environment and guide the required cellular functions for cartilage tissue regeneration. The current advances on tuning the properties of PNIPAm-based scaffolds were searched for on Google Scholar, PubMed, and Web of Science. This review provides a comprehensive overview of the scaffolding properties of PNIPAm-based hydrogels and the effects of synthesis-solvent and crosslinking density on tuning these properties. Finally, the challenges and perspectives of considering these two design variables for developing PNIPAm-based scaffolds are outlined.

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Additive Manufacturing of Biopolymers for Tissue Engineering and Regenerative Medicine: An Overview, Potential Applications, Advancements, and Trends

International Journal of Polymer Science ◽

10.1155/2021/4907027 ◽

2021 ◽

Vol 2021 ◽

pp. 1-20 ◽

Cited By ~ 2

Author(s):

Dhinakaran Veeman ◽

M. Swapna Sai ◽

P. Sureshkumar ◽

T. Jagadeesha ◽

L. Natrayan ◽

...

Keyword(s):

Tissue Engineering ◽

Additive Manufacturing ◽

3D Printing ◽

Regenerative Medicine ◽

Tissue Regeneration ◽

Three Dimensional ◽

Porous Scaffolds ◽

Wide Range ◽

Potential Applications ◽

Pharmaceutical Industries

As a technique of producing fabric engineering scaffolds, three-dimensional (3D) printing has tremendous possibilities. 3D printing applications are restricted to a wide range of biomaterials in the field of regenerative medicine and tissue engineering. Due to their biocompatibility, bioactiveness, and biodegradability, biopolymers such as collagen, alginate, silk fibroin, chitosan, alginate, cellulose, and starch are used in a variety of fields, including the food, biomedical, regeneration, agriculture, packaging, and pharmaceutical industries. The benefits of producing 3D-printed scaffolds are many, including the capacity to produce complicated geometries, porosity, and multicell coculture and to take growth factors into account. In particular, the additional production of biopolymers offers new options to produce 3D structures and materials with specialised patterns and properties. In the realm of tissue engineering and regenerative medicine (TERM), important progress has been accomplished; now, several state-of-the-art techniques are used to produce porous scaffolds for organ or tissue regeneration to be suited for tissue technology. Natural biopolymeric materials are often better suited for designing and manufacturing healing equipment than temporary implants and tissue regeneration materials owing to its appropriate properties and biocompatibility. The review focuses on the additive manufacturing of biopolymers with significant changes, advancements, trends, and developments in regenerative medicine and tissue engineering with potential applications.

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