GC–MS Analysis, Antioxidant and Antimicrobial Activities of Achillea Odorata Subsp. Pectinata and Ruta Montana Essential Oils and Their Potential Use as Food Preservatives

In order to discover new natural resources with biological properties, the chemical composition, the antioxidant and antimicrobial activities, and the potential use as food preservative of essential oils of Moroccan Achillea odorata subsp. pectinata (AOpEO) and Ruta montana (RMEO) were studied. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of 21 and 25 compounds in AOpEO and RMEO, respectively. The results showed that the major compounds of AOpEO are camphor (45.01%), bornyl acetate (15.07%), borneol (11.33%), β-eudesmol (4.74%), camphene (3.58%), and 1.8-cineole (eucalyptol) (2.96%), whereas 2-undecanone (63.97%), camphor (3.82%) and cyclopropanecarboxylic acid (3.66%) were the main components of RMEO. The antioxidant activities were evaluated by diphenylpicrylhydraziyl radical (DPPH) and reducing power assays. The antimicrobial activities of essential oils were tested against bacterial strains and food contaminant yeast using agar disc diffusion and microdilution methods. A significant antimicrobial activity of AOpEO was observed against Bacillus subtilis, Proteus mirabilis and Candida albicans, compared to RMEO. The efficacy of AOpEO was also evaluated in model food systems (cabbage and barley) artificially inoculated during storage. The results found that the adding of a minimal inhibitory concentration (MIC) and 4× MIC were potent in decreasing the Proteus mirabilis growth in food model systems. Our findings suggested that AOpEO may be potentially used as an alternative food preservative.

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Biological Properties of Essential Oils from Thymus algeriensis Boiss

Plants ◽

10.3390/plants10040786 ◽

2021 ◽

Vol 10 (4) ◽

pp. 786

Author(s):

Hamza Ouakouak ◽

Adel Benarfa ◽

Mohammed Messaoudi ◽

Samir Begaa ◽

Barbara Sawicka ◽

...

Keyword(s):

Essential Oils ◽

Micrococcus Luteus ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Antimicrobial Activities ◽

Biological Properties ◽

Chemical Components ◽

Bornyl Acetate ◽

Thymus Algeriensis

This study describes the chemical composition, antitumor, antioxidant, and antimicrobial activities of the plant Thymus algeriensis Boiss. Essential oils (EOs) were collected in different periods (before, during, and after flowering stage) from the El-Guetfa region, M’sila, Algeria. The EOs extraction was achieved using three distinguishing techniques: hydro (Clevenger trap), steam, and microwave distillations, targeting different aerial parts of the plant (stems, flowers, and leaves). The EOs chemical components were estimated using GC-FID and GC-MS apparatuses. The resulting yield of the extracted oil was moderate and ranged between 0.84 and 1.53% (wt/vol). In total, eighty-five components were identified, in which the oxygenated monoterpenes family formed the main portion, starting from 40.56 up to 70.66%. The obtained essential oil was dominated by five major components that varied from low to quite moderate percentages: camphor (17.45–32.56%), borneol (11.16–22.2%), camphene (7.53–12.86%), 1.8-cineole (5.16–11.21%), and bornyl acetate (3.86–7.92%). The biological results of this oil pointed out that the EOs extracted from the leaves part exposed a weak radical scavenging activity afterward using two well-known antioxidant assays DPPH (IC50 = 8.37 mg/mL) and ABTS (10.84 mg/mL). Meanwhile, this oil presented strong inhibition activity against colon cancer cell line HCT116 (LC50 = 39.8 µg/mL) and a moderate inhibitory against hepatocellular cancer cells HePG2 (LC50 > 100 µg/mL). In addition, this oil antimicrobial activity was quite important against Micrococcus luteus (M. luteus), Staphylococcus aureus CIP 7625, Escherichia coli ATCC 10536, Saccharomyces cerevisiae ATCC 4226, Candida albicans IPA200, Candida tropicalis (Ct), and Candida glabrata (Cg) after using Amoxicillin and Itraconazole as references.

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Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

Journal of Chemistry ◽

10.1155/2015/804658 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Kamel Msaada ◽

Nidhal Salem ◽

Olfa Bachrouch ◽

Slim Bousselmi ◽

Sonia Tammar ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Methanolic Extract ◽

Biological Activities ◽

Reducing Power ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

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Volatile Compounds and Antioxidant and Antimicrobial Activities of Selected Citrus Essential Oils Originated from Nepal

Molecules ◽

10.3390/molecules26216683 ◽

2021 ◽

Vol 26 (21) ◽

pp. 6683

Author(s):

Devi Prasad Bhandari ◽

Darbin Kumar Poudel ◽

Prabodh Satyal ◽

Karan Khadayat ◽

Sital Dhami ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Citrus Sinensis ◽

Antioxidant Properties ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Citrus Species ◽

Citrus Peel ◽

Antioxidant And Antimicrobial Activities

Citrus species of plants are among the most commercially cultivated crops, mainly for their fruit. Besides, the generally consumed flesh inside the fruit, the peel is quite important too. Essential oils extracted from the peel have a history of being used by humankind for centuries. These essential oils are rich in antioxidants and antimicrobial agents. Comparative investigation of volatile constituents, and antioxidant and antimicrobial activities were undertaken. The essential oils were evaluated through gas chromatography-mass spectrometry (GC–MS), and enantiomeric composition by chiral GC–MS. Similarly, the antioxidant properties were evaluated by 2,2-diphenyl-1-picrylhydrazyl scavenging assay, and antimicrobial activities were assayed using the disk diffusion method. The highest extraction yield of 1.83% was observed in Citrus sinensis Osbeck. GC–MS analysis showed limonene (63.76–89.15%), γ-terpinene (0.24–6.43%), β-pinene (0.15–6.09%), linalool (0.35–3.5%), sabinene (0.77–2.17%), myrcene (0.74–1.75%), α-terpineol (0.28–1.15%), and α-pinene (0.2–0.58%) as the major constituents of the essential oil of the Citrus species studied. For the first time, through our study, chiral terpenoids have been observed from Citrus grandis Osbeck essential oil. The order of antioxidant activity is as follows: Citrus grandis Osbeck red flesh > Citrus reticulata Blanco > Citrus sinensis Osbeck > Citrus grandis Osbeck white flesh. Except for Citrus grandis Osbeck white flesh (52.34 µL/mL), all samples demonstrated stronger antioxidant activities than those of the positive control, quercetin (5.60 µL/mL). Therefore, these essential oils can be used as a safe natural antioxidant to prevent product oxidation. Likewise, citrus peel essential oil showed antimicrobial activity against tested bacterial strains, albeit marginal.

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Chemical Compositions and Antimicrobial Activity of the Essential Oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae)

Natural Product Communications ◽

10.1177/1934578x1400901235 ◽

2014 ◽

Vol 9 (12) ◽

pp. 1934578X1400901 ◽

Cited By ~ 1

Author(s):

Wan Mohd Nuzul Hakimi Wan Salleh ◽

Farediah Ahmad ◽

Khong Heng Yen

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Antimicrobial Agents ◽

Antimicrobial Activities ◽

Dilution Method ◽

Chemical Compositions ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Ms Analysis ◽

Weak Activity

The study was designed to examine the chemical composition and antimicrobial activities of essential oils extracted from the aerial parts of three Piper species: Piper abbreviatum, P. erecticaule and P. lanatum, all from Malaysia. GC and GC/MS analysis showed qualitative and quantitative differences between these oils. GC and GC-MS analysis of P. abbreviatum, P. erecticaule and P. lanatum oils resulted in the identification of 33, 35 and 39 components, representing 70.5%, 63.4% and 78.2% of the components, respectively. The major components of P. abbreviatum oil were spathulenol (11.2%), ( E)-nerolidol (8.5%) and β-caryophyllene (7.8%), whereas P. erecticaule oil mainly contained β-caryophyllene (5.7%) and spathulenol (5.1%). Borneol (7.5%), β-caryophyllene (6.6%) and α-amorphene (5.6%) were the most abundant components in P. lanatum oil. Antimicrobial activity was carried out using disc diffusion and broth micro-dilution method against nine microorganisms. All of the essential oils displayed weak activity towards Gram-positive bacteria with MIC values in the range 250–500 μg/mL. P. erecticaule oil showed the best activity on Aspergillus niger (MIC 31.3 μg/mL), followed by P. lanatum oil (MIC 62.5 μg/mL). This study demonstrated that the essential oils have potential as antimicrobial agents and may be useful in the pharmaceutical and cosmetics industries.

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MALAGASY AROMATIC PLANTS: ESSENTIAL OILS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES

Acta Horticulturae ◽

10.17660/actahortic.2004.629.9 ◽

2004 ◽

pp. 77-81 ◽

Cited By ~ 8

Author(s):

H.R. Juliani ◽

J.E. Simon ◽

M.M.R. Ramboatiana ◽

O. Behra ◽

A. Garvey ◽

...

Keyword(s):

Essential Oils ◽

Antimicrobial Activities ◽

Aromatic Plants ◽

Antioxidant And Antimicrobial Activities

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Variability in essential oil composition, antioxidant and antimicrobial activities of Ruta montana L. collected from different geographical regions in Algeria

Journal of Essential Oil Research ◽

10.1080/10412905.2019.1660238 ◽

2019 ◽

Vol 32 (1) ◽

pp. 88-101 ◽

Cited By ~ 3

Author(s):

Hichem Mohammedi ◽

Samira Mecherara-Idjeri ◽

Aicha Hassani

Keyword(s):

Essential Oil ◽

Antimicrobial Activities ◽

Essential Oil Composition ◽

Oil Composition ◽

Geographical Regions ◽

Ruta Montana ◽

Antioxidant And Antimicrobial Activities

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Phytochemistry, antioxidant and antimicrobial activities of the essential oils of Mentha rotundifolia L. in Tunisia

Industrial Crops and Products ◽

10.1016/j.indcrop.2013.06.032 ◽

2013 ◽

Vol 49 ◽

pp. 883-889 ◽

Cited By ~ 52

Author(s):

Leila Riahi ◽

Myriam Elferchichi ◽

Hanene Ghazghazi ◽

Jed Jebali ◽

Sana Ziadi ◽

...

Keyword(s):

Essential Oils ◽

Antimicrobial Activities ◽

Antioxidant And Antimicrobial Activities

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Chemical Compositions, Antioxidant and Antimicrobial Activities of Essential Oils of Piper caninum Blume

International Journal of Molecular Sciences ◽

10.3390/ijms12117720 ◽

2011 ◽

Vol 12 (11) ◽

pp. 7720-7731 ◽

Cited By ~ 22

Author(s):

Wan Mohd Nuzul Hakimi Wan Salleh ◽

Farediah Ahmad ◽

Khong Heng Yen ◽

Hasnah Mohd Sirat

Keyword(s):

Essential Oils ◽

Antimicrobial Activities ◽

Chemical Compositions ◽

Antioxidant And Antimicrobial Activities

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Antibacterial and Antibiofilm Activities of Cinnamomum Sp. Essential Oil and Cinnamaldehyde: Antimicrobial Activities

The Scientific World JOURNAL ◽

10.1155/2018/7405736 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Diego F. Firmino ◽

Theodora T. A. Cavalcante ◽

Geovany A. Gomes ◽

Nairley C. S. Firmino ◽

Lucas D. Rosa ◽

...

Keyword(s):

Essential Oils ◽

Antimicrobial Activities ◽

Bacterial Biofilms ◽

Antibiofilm Activity ◽

Cinnamomum Zeylanicum ◽

Microdilution Method ◽

Viable Cells ◽

Broth Microdilution Method ◽

Clinical Interest ◽

Potential Use

To assess the activities of essential oils derived from the trunk bark of Cinnamomum zeylanicum (EOCz) and Cinnamomum cassia (EOCc) as well as cinnamaldehyde on bacterial biofilms of clinical interest. Antimicrobial activity was assessed by the broth microdilution method to determine minimum inhibitory concentrations (MICs). Antibiofilm activity was assessed by quantifying the biomass and determining the number of viable cells. The chemical composition of the essential oils was determined. The results showed that the major component of EOCz and EOCc was cinnamaldehyde. For the assayed substances, biofilm biomasses were reduced by up to 99.9%, and Streptococcus pyogenes, Pseudomonas aeruginosa, and Escherichia coli biofilms were sensitive to all of the concentrations and substances analysed. In cell viability tests, 2 mg/ml of cinnamaldehyde reduced the number of viable cells by 5.74 Log CFU/ml. EOCz, EOCc, and cinnamaldehyde exhibited antimicrobial and antibiofilm activities. This work describes substances with potential use against infections caused by bacterial biofilms.

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