scholarly journals SMRT and Illumina RNA Sequencing and Characterization of a Key NAC Gene LoNAC29 during the Flower Senescence in Lilium oriental ‘Siberia’

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 869
Author(s):  
Jing Luo ◽  
Ruirui Li ◽  
Xintong Xu ◽  
Hairui Niu ◽  
Yujie Zhang ◽  
...  
Keyword(s):  
Single Molecule ◽  
Sequence Data ◽  
Economic Loss ◽  
Flower Senescence ◽  
Cut Flower ◽  
Transient Overexpression ◽  
Flower Quality ◽  
New Evidence ◽  
Senescence Associated Genes

Lily (Lilium spp.) is an important cut flower around the world. Flower senescence in lilies is characterized by the wilting and abscission of tepals, which results in a decrease in flower quality and huge economic loss. However, the mechanism underlying flower senescence in lilies is largely unknown. In this study, single-molecule, real-time (SMRT) and Illumina sequencing were carried out in L. oriental ‘Siberia’. Sequencing yielded 73,218 non-redundant transcripts, with an N50 of 3792 bp. These data were further integrated with three published transcriptomes through cogent analysis, which yielded 62,960 transcripts, with an increase in N50 of 3935 bp. Analysis of differentially expressed genes showed that 319 transcription factors were highly upregulated during flower senescence. The expression of twelve NAC genes and eleven senescence-associated genes (SAGs) showed that LoNAC29 and LoSAG39 were highly expressed in senescent flowers. Transient overexpression of LoNAC29 and LoSAG39 in tepals of lily notably accelerated flower senescence, and the promoter activity of LoSAG39 was strongly induced by LoNAC29. This work supported new evidence for the molecular mechanism of flower senescence and provided better sequence data for further study in lilies.

scholarly journals Characterization of a clinical Clostridioides difficile isolate with markedly reduced fidaxomicin susceptibility and a V1143D mutation in rpoB

2018 ◽  
Vol 74 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Julian Schwanbeck ◽  
Thomas Riedel ◽  
Friederike Laukien ◽  
Isabel Schober ◽  
Ines Oehmig ◽  
...  
Keyword(s):  
Single Molecule ◽  
Sequence Data ◽  
Normal Growth ◽  
Amplicon Sequencing ◽  
Spore Formation ◽  
Compensatory Mechanisms ◽  
Toxin A ◽  
Clostridioides Difficile

Abstract Objectives The identification and characterization of clinical Clostridioides difficile isolates with reduced fidaxomicin susceptibility. Methods Agar dilution assays were used to determine fidaxomicin MICs. Genome sequence data were obtained by single-molecule real-time (SMRT) sequencing in addition to amplicon sequencing of rpoB and rpoC alleles. Allelic exchange was used to introduce the identified mutation into C. difficile 630Δerm. Replication rates, toxin A/B production and spore formation were determined from the strain with reduced fidaxomicin susceptibility. Results Out of 50 clinical C. difficile isolates, isolate Goe-91 revealed markedly reduced fidaxomicin susceptibility (MIC >64 mg/L). A V1143D mutation was identified in rpoB of Goe-91. When introduced into C. difficile 630Δerm, this mutation decreased fidaxomicin susceptibility (MIC >64 mg/L), but was also associated with a reduced replication rate, low toxin A/B production and markedly reduced spore formation. In contrast, Goe-91, although also reduced in toxin production, showed normal growth rates and only moderately reduced spore formation capacities. This indicates that the rpoBV1143D allele-associated fitness defect is less pronounced in the clinical isolate. Conclusions To the best of our knowledge, this is the first description of a pathogenic clinical C. difficile isolate with markedly reduced fidaxomicin susceptibility. The lower-than-expected fitness burden of the resistance-mediating rpoBV1143D allele might be an indication for compensatory mechanisms that take place during in vivo selection of mutants.

scholarly journals Effects of Silver Thiosulfate, Sucrose, and Calcium Nitrate on Vaselife of Ebenus cretica L.

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 460A-460
Author(s):  
Apostolos A. Paralikas ◽  
J.C. Vlahos ◽  
M. Papadimitriou ◽  
K.A. Loulakakis
Keyword(s):  
Calcium Nitrate ◽  
Flower Senescence ◽  
Cut Flower ◽  
Flower Longevity ◽  
Silver Thiosulfate ◽  
Sucrose Solutions ◽  
Leaf Yellowing ◽  
Chlorophyll A And B ◽  
Exogenous Ethylene ◽  
Flower Quality

Ebenus cretica, Leguminosae, an endemic perennial bush of Crete, is being studied as a potential new cut flower crop. Forty-centimeter-long spikes with two to three inflorescences and six to eight compound leaves were harvested from 5-year-old plants grown from seed at the farm of the TEI, when 1/3 of the florets had opened, and were treated with various preservatives. Flower quality was evaluated morphologically combined with measurements of chlorophyll content in leaves and anthocyanin in petals. Without any postharvest treatments, inflorescences held in a solution of 100 ppm 8-hydroxyquinone sulfate (HQS) in DI water had an average vaselife of 6.8 days. Pulsing with 0.6 mM silver thiosulfate (STS) for 2 h extended vaselife up to 8.4 days. However, when ethephon was added in the solution, vaselife was significantly reduced, causing leaf yellowing and flower senescence, which suggests sensitivity to exogenous ethylene. A solution of 0.2% Ca(NO3)2 prolonged vaselife by 2.7 days, whereas higher concentrations resulted in flower discoloration and decreased flower quality. Sucrose solutions of 0.5%, 1%, 2%, and 4% had no positive effect on flower longevity. Furthermore, the higher concentrations caused leaf yellowing and petal discoloration decreasing vaselife and quality of flowers compared to control. Samples of inflorescences were taken every second day for chlorophyll (a and b) and anthocyanin measurements. The concentrations recorded were highest in the 0.2% Ca(NO3)2 treatment and were significantly correlated to flower longevity. Results indicate that Ebenus cretica may be used as a cut flower crop; however, due to the genetic variability of the Ebenus plants, a breeding line should be developed before the crop reaches the floricultural market.

Effects of different concentrations of sucrose and citric acid on vase life of rose

2019 ◽  
Vol 2 (1) ◽  
pp. 127-134 ◽  
Author(s):  
Pragya Aryal ◽  
Anjila Adhikari ◽  
Rukmangat Pathak ◽  
Rameshwor Pudasaini
Keyword(s):  
Citric Acid ◽  
Technical College ◽  
Flower Senescence ◽  
Vase Life ◽  
Cut Flower ◽  
Citric Acid Solution ◽  
Flower Bud ◽  
Flower Quality ◽  
Cut Rose

Vase life is the most important parameter to determine the quality of cut flower, however, due to highly perishable nature is always challenging to vase life. An experiment was conducted at Horticulture Lab, Prithu Technical College, Dangon 2018 in order to find out best concentration of sucrose and citric solution that enhances and prolongs the better flower quality and longevity. Experiment was laid out in completely randomize design (CRD) with nine treatments and three replication. Rose sticks were harvested at flower bud stage and two sticks were kept in each vase solution. 2% sucrose with 15 ppm citric acid solution found longest vase life and this combination has the potential to be used as a commercial cut flower preservative solution to delay flower senescence, enhance post-harvest quality and prolong the vase life of cut rose flowers.

Multidimensional Characterization of Single‐Molecule Dynamics in a Plasmonic Nanocavity

2021 ◽  
Author(s):  
Lucas Domulevicz ◽  
Hyunhak Jeong ◽  
Nayan K. Paul ◽  
Juan Sebastian Gomez-Diaz ◽  
Joshua Hihath
Keyword(s):  
Single Molecule ◽  
Single Molecule Dynamics ◽  
Molecule Dynamics

scholarly journals Sequencing and Computational Approaches to Identification and Characterization of Microbial Organisms

2013 ◽  
Vol 5 ◽  
pp. BECB.S10886 ◽  
Author(s):  
Brijesh Singh Yadav ◽  
Venkateswarlu Ronda ◽  
Dinesh P. Vashista ◽  
Bhaskar Sharma
Keyword(s):  
Sequence Data ◽  
Microbial Interactions ◽  
Microbial Pathogens ◽  
Nucleotide Sequence Data ◽  
Computational Approaches ◽  
Microbial Detection ◽  
Sequencing Technologies ◽  
Sequencing Platforms ◽  
Identification And Characterization

The recent advances in sequencing technologies and computational approaches are propelling scientists ever closer towards complete understanding of human-microbial interactions. The powerful sequencing platforms are rapidly producing huge amounts of nucleotide sequence data which are compiled into huge databases. This sequence data can be retrieved, assembled, and analyzed for identification of microbial pathogens and diagnosis of diseases. In this article, we present a commentary on how the metagenomics incorporated with microarray and new sequencing techniques are helping microbial detection and characterization.

Single-molecule binding characterization of primosomal protein PriA involved in replication restart

2021 ◽  
Author(s):  
Tzu-Yu Lee ◽  
Yi-Ching Li ◽  
Min-Guan Lin ◽  
Chwan-Deng Hsiao ◽  
Hung-Wen Li
Keyword(s):  
Dna Replication ◽  
Dna Synthesis ◽  
Single Molecule ◽  
Replication Forks ◽  
Bacterial Survival ◽  
Dna Damages ◽  
Replication Restart

DNA damages lead to stalled or collapsed replication forks. Replication restart primosomes re-initiate DNA synthesis at these stalled or collapsed DNA replication forks, which is important for bacterial survival. Primosomal...

scholarly journals Single Molecule FRET Characterization of Oligomers from Alpha-Synuclein Early Onset Parkinson's Disease Mutants

2014 ◽  
Vol 106 (2) ◽  
pp. 269a ◽  
Author(s):  
Laura Tosatto ◽  
Mathew H. Horrocks ◽  
Cremades Nunilo ◽  
Tim Guilliams ◽  
Mauro Dalla Serra ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Single Molecule ◽  
Early Onset ◽  
Alpha Synuclein ◽  
Single Molecule Fret ◽  
Early Onset Parkinson’S Disease

scholarly journals Characterization of Streptomyces scabies isolates from potato tubers its pathogenic variations in Rawalpindi District

2014 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
S. Shazia ◽  
F. Naz ◽  
A. Rauf ◽  
M. Inam-ul-Haq ◽  
S. Bushra
Keyword(s):  
Solanum Tuberosum L ◽  
Economic Loss ◽  
Streptomyces Scabies ◽  
Potato Tubers ◽  
Yield And Quality ◽  
Isolation And Characterization ◽  
Pathogenic Variation ◽  
Important Disease

Potato (Solanum tuberosum L.) has various biotic constraints in its production due to pest attack. Among these, common scab caused by streptomyces scabies is an important disease in potato which causes economic loss with respect to plant yield and quality of tubers. The present study was conducted to determine the pathogenicity, pathogenic variation, characterization of morphological, physiological and biochemical aspects of Streptomyces associated with potato tubers grown in Rawalpindi district. Severity data and pathogenic variation of disease was studied by using different isolation and characterization techniques.

Sign in / Sign up

Export Citation Format

Share Document