scholarly journals Evolution of Plant B Chromosome Enriched Sequences

Genes ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 515 ◽  
Author(s):  
André Marques ◽  
Sonja Klemme ◽  
Andreas Houben

B chromosomes are supernumerary chromosomes found in addition to the normal standard chromosomes (A chromosomes). B chromosomes are well known to accumulate several distinct types of repeated DNA elements. Although the evolution of B chromosomes has been the subject of numerous studies, the mechanisms of accumulation and evolution of repetitive sequences are not fully understood. Recently, new genomic approaches have shed light on the origin and accumulation of different classes of repetitive sequences in the process of B chromosome formation and evolution. Here we discuss the impact of repetitive sequences accumulation on the evolution of plant B chromosomes.

Author(s):  
André Marques ◽  
Sonja Klemme ◽  
Andreas Houben

B chromosomes are supernumerary chromosomes which are found in addition to the normal standard chromosomes (A chromosomes). B chromosomes are well known to accumulate several types of repeats. Although the evolution of B chromosomes has been subject of numerous studies, the mechanisms of accumulation and evolution of repetitive sequences is not fully understood. Recently, new genomic approaches have shed light on the origin and accumulation of different classes of repetitive sequences in the process of B chromosome formation and evolution. Here we discuss the impact of repetitive sequences accumulation on the evolution of plant B chromosomes.


Genes ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 509 ◽  
Author(s):  
Ilyas Jetybayev ◽  
Alexander Bugrov ◽  
Victoria Dzuybenko ◽  
Nikolay Rubtsov

B chromosomes (Bs) were described in most taxa of eukaryotes and in around 11.9% of studied Orthopteran species. In some grasshopper species, their evolution has led to many B chromosome morphotypes. We studied the Bs in nine species (Nocaracris tardus, Nocaracris cyanipes, Aeropus sibiricus, Chorthippus jacobsoni, Chorthippus apricarius, Bryodema gebleri, Asiotmethis heptapotamicus songoricus, Podisma sapporensis, and Eyprepocnemis plorans), analyzing their possible origin and further development. The studied Bs consisted of C-positive or C-positive and C-negative regions. Analyzing new data and considering current hypotheses, we suggest that Bs in grasshoppers could arise through different mechanisms and from different chromosomes of the main set. We gave our special attention to the Bs with C-negative regions and suggest a new hypothesis of B chromosome formation from large or medium autosomes. This hypothesis includes dissemination of repetitive sequences and development of intercalary heterochromatic blocks in euchromatic chromosome arm followed by deletion of euchromatic regions located between them. The hypothesis is based on the findings of the Eyprepocnemis plorans specimens with autosome containing numerous intercalary repeat clusters, analysis of C-positive Bs in Eyprepocnemis plorans and Podisma sapporensis containing intercalary and terminal C-negative regions, and development of heterochromatic neo-Y chromosome in some Pamphagidae grasshoppers.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rahman Ebrahimzadegan ◽  
Andreas Houben ◽  
Ghader Mirzaghaderi

AbstractHere, we characterized the basic properties of repetitive sequences in essential A and supernumerary B chromosomes of Festuca pratensis Huds. This was performed by comparative analysis of low-pass Illumina sequence reads of B chromosome lacking (−B) and B chromosome containing (+B) individuals of F. pratensis. 61% of the nuclear genome is composed of repetitive sequences. 43.1% of the genome are transposons of which DNA transposons and retrotransposons made up 2.3% and 40.8%, respectively. LTR retrotransposons are the most abundant mobile elements and contribute to 40.7% of the genome and divided into Ty3-gypsy and Ty1-copia super families with 32.97% and 7.78% of the genome, respectively. Eighteen different satellite repeats were identified making up 3.9% of the genome. Five satellite repeats were used as cytological markers for chromosome identification and genome analysis in the genus Festuca. Four satellite repeats were identified on B chromosomes among which Fp-Sat48 and Fp-Sat253 were specific to the B chromosome of F. pratensis.


2020 ◽  
Vol 228 ◽  
pp. 00019
Author(s):  
Frédérick Poidevin

The Davis-Chandrasekhar-Fermi (DCF) method using the Angular Dispersion Function (ADF), the Histogram of Relative Orientations (HROs) and the Polarization-Intensity Gradient Relation (P-IGR) are the most common tools used to analyse maps of linearly polarized emission by thermal dust grains at submilliter wavelengths in molecular clouds and star-forming regions. A short review of these methods is given. The combination of these methods will provide valuable tools to shed light on the impact of the magnetic fields on the formation and evolution of subparsec scale hub-filaments that will be mapped with the NIKA2 camera and future experiments.


2020 ◽  
Vol 160 (3) ◽  
pp. 111-117
Author(s):  
Cesar Martins ◽  
Vladimir Trifonov ◽  
Andreas Houben

B chromosomes (Bs) are enigmatic accessory genomic elements extensively characterized in diverse eukaryotes. Since their discovery in the beginning of the 20th century, B chromosomes have been the subject of investigation in laboratories all around the world. As a consequence, scientific meetings have dealt with B chromosomes, including the most specific and important conference in the field, “The B Chromosome Conference.” The 4th B Chromosome Conference (4BCC) took place in Botucatu, Brazil, in 2019 and was an excellent opportunity to discuss the latest developments in the B chromosome research field. B chromosome science has advanced from classical and molecular cytogenetics to genomics and bioinformatics approaches. The recent advances in next-generation sequencing technologies and high-throughput molecular biology protocols have led Bs to be the subject of massive data analysis, thus enabling the investigation of structural and functional issues not considered before. Although extensive progress has been made, questions are still remaining to be answered. The advances in functional studies based on RNA, epigenetics, and gene ontologies open the perspective to a better understanding of the complex biology of B chromosomes.


2016 ◽  
Vol 148 (1) ◽  
pp. 68-73 ◽  
Author(s):  
Katrin Kumke ◽  
Jiří Macas ◽  
Jörg Fuchs ◽  
Lothar Altschmied ◽  
Jasmeet Kour ◽  
...  

B chromosomes are supernumerary dispensable parts of the karyotype which appear in some individuals of some populations in some species. Using advanced sequencing technology, we in silico characterized the high-copy DNA composition of Plantago lagopus with and without B chromosomes. The nuclear genome (2.46 pg/2C) was found to be relatively rich in repetitive sequences, with highly and moderately repeated elements making up 68% of the genome. Besides a centromere-specific marker, we identified a B-specific satellite and a repeat enriched in polymorphic A chromosome segments. The B-specific tandem repeat PLsatB originated from sequence amplification including 5S rDNA fragments.


2018 ◽  
Vol 154 (2) ◽  
pp. 79-85
Author(s):  
Amanda A. Soares ◽  
Jonathan P. Castro ◽  
Pedro Balieiro ◽  
Sidnei Dornelles ◽  
Tiago M. Degrandi ◽  
...  

B chromosomes are supernumerary chromosomes found in the karyotypes of approximately 15% of all eukaryotic species. They present parasitic behavior and do not follow the standard Mendelian pattern of inheritance, resulting in an imbalance in gametogenesis. The evolutionary dynamics of B chromosomes is still unknown for many species, but studies indicate that the accumulation of repetitive sequences plays an important role in the differentiation of these elements. We analyzed morphology, frequency, and possible homologies amongst different B chromosomes found in an isolated Akodon montensis population in southern Brazil. Repetitive sequences (18S, 5S rDNA and telomeric sequences) were used to test for their accumulation on the supernumerary chromosomes and describe their localization in the species. The results indicate 4 different B chromosome morphotypes, and DNA libraries were generated for 3 of them. 18S rDNA was labelled polymorphically, except in the B chromosomes, whereas the 5S rDNA was located exclusively in an interstitial position on the long arm of chromosome 5. Chromosome painting with the B probes based on FISH revealed a homologous composition for all B chromosome morphotypes and no homology with the chromosomes in the A complement. B chromosomes found in this population may have a common origin and subsequently diversified in size and morphology.


wisdom ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 45-55
Author(s):  
Natalya KUTUZA ◽  
Inna Kravets

The article deals with the phenomenon of communicative influence, where the main attention is paid to its theoretical consideration in linguistics and philosophy. The subject of the study was the theoretical basis of communicative influence. The purpose of the proposed article is to consider communicative influence in linguophilosophical aspect. A number of research methods have been used to achieve this goal: descriptive to shed light on the nature of communicative influence; specifics of the review of influence in philosophy and linguistics; a method of analysis and synthesis for the identification of the components of communicative influence and the combination of their single complex; modeling method for constructing the degree of suggestiveness expression; the induction method served to conclude the general conclusions. It is found that philosophers have focused their attention on the "magic" of the word when studying influence; close connection of spirit, soul with language, psychophysiological phenomena; inaccuracies and variability of interpretation of reality; language as activity, motives for action. In the linguistic aspect, in the light of new and emerging disciplines, attention is focused on the variability, imagery, associative potential of lexemes and linguistic complexes, which is a suggestogenic potential, which is analyzed at all linguistic levels. It is emphasized that language is generally a suggestive phenomenon. The authors propose to consider the impact actualizers (suggestems / suggestogens) according to the degree of suggestiveness manifestation, where implementation at two language levels is poor actualization, at three - moderate, more than three - strong.


2020 ◽  
pp. 1-21
Author(s):  
Emily H. Hull

Abstract Osteobiographies are a common form of presenting the archaeological analysis of the life history of an individual. This form of analysis, however, is usually reserved for human subjects. Writing an osteobiography of a nonhuman person is complicated by the lack of human understanding of animal thought and experience. Such analysis is further complicated when the subject is not a companion animal, and isolated from human funerary rituals which may shed light on the animal’s life. The skeletal remains of an injured wild caribou from Alberta who was collected as a museum specimen presents a unique opportunity to understand an individual animal’s life, as well presents an example of the complexities of human-animal relationships in an analytical setting. This study examines both the life of an extraordinary nonhuman person and the impact of reconstructing nonhuman life histories on the analyst.


2017 ◽  
Vol 152 (4) ◽  
pp. 213-221 ◽  
Author(s):  
Andrezza C.S. Bernardino ◽  
Diogo C. Cabral-de-Mello ◽  
Carolina B. Machado ◽  
Octavio M. Palacios-Gimenez ◽  
Neide Santos ◽  
...  

B chromosomes, extra elements present in the karyotypes of some eukaryote species, have been described in the grasshopper Xyleus discoideus angulatus. Although some studies have proposed an autosomal origin of the B chromosome in X. d. angulatus, little is known about its repetitive DNA composition and evolutionary dynamics. The aim of the present work was to shed light on the B chromosome evolution in X. d. angulatus by cytogenetic analysis of 27 populations from Pernambuco and Ceará states (Brazil). The frequency of B chromosomes in the different populations was determined, and chromosome measurements and fluorescence in situ hybridization (FISH) with C0t-DNA and telomeric and B chromosome sequences were performed in cells from B-carrying individuals. The results revealed variations in B chromosome prevalence among the populations and showed that some B chromosomes were smaller in certain populations. FISH produced similar patterns for the C0t-DNA probe in all hybridized individuals, whereas telomeric and B chromosome probes, obtained by microdissection, exhibited variations in their distribution. These results indicate the presence of 3 morphotypes of B chromosomes in X. d. angulatus, with variation in repetitive DNA composition during their evolution. In this species, B chromosomes have an intraspecific origin and probably arose from the pericentromeric region of A chromosomes.


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