Molecular Evidence of Mucoromycotina “Fine Root Endophyte” Fungi in Agricultural Crops

AbstractNon-vascular plants associating with arbuscular mycorrhizal (AMF) and Mucoromycotina ‘fine root endophyte’ (MFRE) fungi derive greater benefits from their fungal associates under higher atmospheric [CO2] (a[CO2]) than ambient; however, nothing is known about how changes in a[CO2] affect MFRE function in vascular plants. We measured movement of phosphorus (P), nitrogen (N) and carbon (C) between the lycophyte Lycopodiella inundata and Mucoromycotina fine root endophyte fungi using 33P-orthophosphate, 15 N-ammonium chloride and 14CO2 isotope tracers under ambient and elevated a[CO2] concentrations of 440 and 800 ppm, respectively. Transfers of 33P and 15 N from MFRE to plants were unaffected by changes in a[CO2]. There was a slight increase in C transfer from plants to MFRE under elevated a[CO2]. Our results demonstrate that the exchange of C-for-nutrients between a vascular plant and Mucoromycotina FRE is largely unaffected by changes in a[CO2]. Unravelling the role of MFRE in host plant nutrition and potential C-for-N trade changes between symbionts under different abiotic conditions is imperative to further our understanding of the past, present and future roles of plant-fungal symbioses in ecosystems.

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Mucoromycotina Fine Root Endophyte Fungi Form Nutritional Mutualisms with Vascular Plants

PLANT PHYSIOLOGY ◽

10.1104/pp.19.00729 ◽

2019 ◽

Vol 181 (2) ◽

pp. 565-577 ◽

Cited By ~ 13

Author(s):

Grace A. Hoysted ◽

Alison S. Jacob ◽

Jill Kowal ◽

Philipp Giesemann ◽

Martin I. Bidartondo ◽

...

Keyword(s):

Vascular Plants ◽

Fine Root ◽

Root Endophyte ◽

Endophyte Fungi

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Carbon for nutrient exchange between the lycophyte, Lycopodiella inundata and Mucoromycotina ‘fine root endophytes’ is unresponsive to high atmospheric CO2 concentration

10.1101/2020.09.29.318022 ◽

2020 ◽

Author(s):

Grace A. Hoysted ◽

Jill Kowal ◽

Silvia Pressel ◽

Jeffrey G. Duckett ◽

Martin I. Bidartondo ◽

...

Keyword(s):

Vascular Plants ◽

Fine Root ◽

Vascular Plant ◽

Arbuscular Mycorrhizal ◽

Co2 Concentration ◽

Root Endophyte ◽

Isotope Tracers ◽

Fungal Associates ◽

Endophyte Fungi

Background and AimsNon-vascular plants associating with arbuscular mycorrhizal (AMF) and Mucoromycotina ‘fine root endophyte’ (MFRE) fungi derive greater benefits from their fungal associates under higher atmospheric [CO2] than ambient, however nothing is known about how changes in [CO2] affects MFRE function in vascular plants.MethodsWe measured movement of phosphorus (P), nitrogen (N) and carbon (C) between the lycophyte, Lycopodiella inundata and Mucoromycotina fine root endophyte fungi using 33P-orthophosphate, 15N-ammonium chloride and 14CO2 isotope tracers under ambient and elevated atmospheric [CO2] concentrations of 440 and 800 ppm, respectively.Key ResultsTransfer of 33P and 15N from MFRE to plant were unaffected by changes in [CO2]. There was a slight increase in C transfer from plant to MFRE under elevated [CO2].ConclusionsOur results demonstrate that the exchange of C-for-nutrients between a vascular plant and Mucoromycotina FRE is largely unaffected by changes in atmospheric [CO2]. Unravelling the role of MFRE in host plant nutrition and potential C-for-N trade changes between symbionts under varying abiotic conditions is imperative to further our understanding of the past, present and future roles of diverse plant-fungal symbioses in global ecosystems.

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Mucoromycotina fine root endophyte fungi form nutritional mutualisms with vascular plants

10.1101/531103 ◽

2019 ◽

Cited By ~ 1

Author(s):

Grace A. Hoysted ◽

Alison S. Jacob ◽

Jill Kowal ◽

Philipp Giesemann ◽

Martin I. Bidartondo ◽

...

Keyword(s):

Vascular Plants ◽

Fine Root ◽

Vascular Plant ◽

Terrestrial Ecosystems ◽

Flowering Plants ◽

Biogeochemical Processes ◽

Root Endophyte ◽

Nitrogen Exchange ◽

Endophyte Fungi

AbstractFungi and plants have engaged in intimate symbioses that are globally widespread and have driven terrestrial biogeochemical processes since plant terrestrialisation >500 Mya. Recently, hitherto unknown nutritional mutualisms involving ancient lineages of fungi and non-vascular plants have been discovered. However, their extent and functional significance in vascular plants remains uncertain. Here, we provide first evidence of abundant carbon-for-nitrogen exchange between an early-diverging vascular plant (Lycopodiaceae) and Mucoromycotina (Endogonales) fine root endophyte regardless of changes in atmospheric CO2 concentration. Furthermore, we provide evidence that the same fungi also colonize neighbouring non-vascular and flowering plants. These findings fundamentally change our understanding of the evolution, physiology, interrelationships and ecology of underground plant-fungal symbioses in terrestrial ecosystems by revealing an unprecedented nutritional role of Mucoromycotina fungal symbionts in vascular plants.

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A new genus, Planticonsortium (Mucoromycotina), and new combination (P. tenue), for the fine root endophyte, Glomus tenue (basionym Rhizophagus tenuis)

Mycorrhiza ◽

10.1007/s00572-017-0815-7 ◽

2018 ◽

Vol 28 (3) ◽

pp. 213-219 ◽

Cited By ~ 23

Author(s):

Christopher Walker ◽

Armelle Gollotte ◽

Dirk Redecker

Keyword(s):

New Genus ◽

Fine Root ◽

New Combination ◽

Root Endophyte ◽

Glomus Tenue

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Sample storage conditions alter colonisation structures of arbuscular mycorrhizal fungi and, particularly, fine root endophyte

Plant and Soil ◽

10.1007/s11104-016-2867-4 ◽

2016 ◽

Vol 412 (1-2) ◽

pp. 35-42 ◽

Cited By ~ 17

Author(s):

Suzanne Orchard ◽

R. J. Standish ◽

D. Nicol ◽

I. A. Dickie ◽

M. H. Ryan

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Fine Root ◽

Arbuscular Mycorrhizal ◽

Storage Conditions ◽

Sample Storage ◽

Root Endophyte

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Impact of plant breeding on genetic diversity of agricultural crops: searching for molecular evidence

Plant Genetic Resources ◽

10.1079/pgr2006116 ◽

2006 ◽

Vol 4 (1) ◽

pp. 71-78 ◽

Cited By ~ 18

Author(s):

Yong-Bi Fu

Keyword(s):

Genetic Diversity ◽

Plant Breeding ◽

Genetic Background ◽

Genetic Improvement ◽

Agricultural Practices ◽

Molecular Evidence ◽

Agricultural Crops ◽

Crop Genetic Diversity ◽

Genome Wide ◽

The Impact

There is a long-standing concern that modern plant breeding reduces crop genetic diversity, which may have consequences for the vulnerability of crops to changes in pests, diseases, climate and agricultural practices. Recent molecular assessments of genetic diversity changes in existing genepools of major agricultural crops may shed some light on the impact of plant breeding on crop genetic diversity. Reviewing published assessments revealed different impacts of plant breeding on improved genepools, not only narrowing or widening their genetic base, but also shifting their genetic background. In general, the genome-wide reduction of crop genetic diversity accompanying genetic improvement over time is minor, but allelic reduction at individual chromosomal segments is substantial. More efforts are needed to assess what proportion of lost alleles is associated with undesirable traits.

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Prevalence and phenology of fine root endophyte colonization across populations of Lycopodiella inundata

Mycorrhiza ◽

10.1007/s00572-020-00979-3 ◽

2020 ◽

Vol 30 (5) ◽

pp. 577-587 ◽

Cited By ~ 1

Author(s):

Jill Kowal ◽

Elena Arrigoni ◽

Jordi Serra ◽

Martin Bidartondo

Keyword(s):

Fine Root ◽

Root Endophyte

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The response of fine root endophyte (Glomus tenue) to waterlogging is dependent on host plant species and soil type

Plant and Soil ◽

10.1007/s11104-016-2804-6 ◽

2016 ◽

Vol 403 (1-2) ◽

pp. 305-315 ◽

Cited By ~ 19

Author(s):

S. Orchard ◽

R. J. Standish ◽

D. Nicol ◽

V. V. S. R. Gupta ◽

M. H. Ryan

Keyword(s):

Host Plant ◽

Plant Species ◽

Soil Type ◽

Fine Root ◽

Root Endophyte ◽

Host Plant Species ◽

Glomus Tenue

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Epipremnum aureum (Araceae) roots associated simultaneously with Glomeromycotina, Mucoromycotina and Ascomycota fungi

Botanica Complutensis ◽

10.5209/bocm.72399 ◽

2021 ◽

Vol 45 ◽

pp. e72399

Author(s):

Thangavelu Muthukumar ◽

Shanmugam Karthik

Keyword(s):

Adventitious Roots ◽

Fine Root ◽

Root Zone ◽

Arbuscular Mycorrhizal ◽

Root Endophyte ◽

Epipremnum Aureum ◽

Aerial Roots ◽

Wide Range ◽

First Time ◽

Endophytic Association

Only a small number of aroids are examined for their symbiosis with glomeromycotean arbuscular mycorrhizal (GAM) fungi and the ascomycetous dark septate endophytic (DSE) fungi. Therefore we examined the aerial and terrestrial adventitious roots of Epipremnum aureum for the endophytic association and the soils for GAM spores. The aerial roots of E. aureum were free from fungal structures, whereas the terrestrial roots were colonized by GAM, fine root endophyte (FRE), and DSE fungi. The major portion of the terrestrial roots was colonized by FRE fungi followed by GAM and DSE fungi. The colonization pattern was a complex of Arum-Paris and intermediate types. Spores of Acaulospora, Funneliformis, Rhizophagus, Rhizophagus and Sclerocystis were isolated from the root zone soils. The results show that E. aureum can establish symbiosis with a wide range of endophytic fungi and FRE symbiosis is reported for the first time in aroids.

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