scholarly journals Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro

2010 ◽  
Vol 11 (10) ◽  
pp. 3793-3802 ◽  
Author(s):  
Alejandra Hernández-Ceruelos ◽  
Eduardo Madrigal-Santillán ◽  
José Antonio Morales-González ◽  
Germán Chamorro-Cevallos ◽  
Martha Cassani-Galindo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Capacity ◽  
Antigenotoxic Effect

scholarly journals Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Anita Agnieszka Śliwińska ◽  
Katarzyna Sykłowska-Baranek ◽  
Anita Kośmider ◽  
Sebastian Granica ◽  
Karolina Miszczak ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Cytotoxic Activity ◽  
Phenolic Compound ◽  
Plant Extracts ◽  
Detrimental Effect ◽  
A549 Cells ◽  
Ferulic Acids

<p>In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of <em>Polyscias filicifolia</em> was developed. The phenolic compound content in <em>P. filicifolia</em> has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of <em>P. filicifolia</em> extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.</p><p>The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.</p>

Methodological Aspects about Determination of Phenolic Compounds and In Vitro Evaluation of Antioxidant Capacity in the Honey: A Review

2009 ◽  
Vol 5 (4) ◽  
pp. 293-302 ◽  
Author(s):  
Jose Alvarez-Suarez ◽  
Sara Tulipani ◽  
Stefania Romandini ◽  
Alexis Vidal ◽  
Maurizio Battino
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
In Vitro Evaluation ◽  
Methodological Aspects

scholarly journals In Vitro Antifungal Activity of Polysulfides-Rich Essential Oil of Ferula Latisecta Fruits against Human Pathogenic Dermatophytes

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Mehrdad Iranshahi ◽  
Abdolmajid Fata ◽  
Bahareh Emami ◽  
Bibi Mohadeseh Jalalzadeh Shahri ◽  
Bibi Sedigheh Fazly Bazzaz
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Agar Dilution ◽  
Sulfur Containing ◽  
Type Strains ◽  
In Vitro Antifungal Activity

The increase in dermatophytoses and the fact that some patients do not respond well to therapy make it necessary to find new antifungal agents. As part of our ongoing studies on medicinal plants from Iran, we studied antidermatophytic activities of Ferula latisecta essential oil, which had shown considerable antifungal activity in preliminary antimicrobial screening. Antifungal activity was evaluated by determination of MIC values using the agar dilution method on type strains of Candida albicans and dermatophytes. The composition of the oil was characterized by GC and GC/MS analyses. The essential oil was rich in polysulfides (75.2%) and exhibited good activity against Trichophyton rubrum and T. verrucosom for about three weeks, with a MIC value 96 μg/mL. The oil showed antifungal activity, especially against dermatophytes, and the activity is probably related to the sulfur-containing components of the oil. This study has identified that the polysulfides-rich essential oil of Ferula latisecta fruits has activity against a range of human pathogenic dermatophytes, justifying future clinical trials to validate its use as a therapeutic alternative for dermatophytosis.

scholarly journals Determination of in Vitro Antioxidant Enzyme Capacity and Oxidative Stress Levels in Mazı Meşesi (Quercus infectoria)

2021 ◽  
Vol 9 (4) ◽  
pp. 814-817
Author(s):  
İlter Demirhan ◽  
Büşra Çitil ◽  
Mehmet Özyurt ◽  
Meltem Güngör ◽  
Erkan Öner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activities ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Quercus Infectoria

South East Anatolia Region has a large genetic plant diversity due to its physical and different climatic charesteristics. These plants are potential sources of antioxidants that prevent oxidative stress caused by oxygen and photons. In recent years, it has become important to study the antioxidant capacity of many molecules found naturally in foods and biological systems. The reason for this is that it is believed that when the consumption of food rich in antioxidants is increased, the risk of developing different degenerative diseases will be reduced. In this study, it was aimed to measure the antoxidant capacity of Quercus infectoria, G.olivier gal seeds grown in Southeastern Anatolia. Q. infectoria gal seeds from Sanlıurfa province were used in our study. Q. infectoria gal seeds were extracted with water, ethanol and methanol and then antioxidant enzyme activities (catalase and superoxide dismutase) and malondialdehyde levels, which are indicators of oxidative stress were determined by spectrophotometric methods. It was found that the antioxidant capacity (catalase and superoxide dismutase activities) of extracts obtained from ethanol and methanol were higher and their malondialdehyde levels were statistically lower than those obtained from water. However, it was determined that there was no statistically significant difference between the antioxidant capacity and malondialdehyde levels of the extracts obtained from methanol compared to the extracts obtained from ethanol. It has been concluded that Q. infectoria gal seed has a effective antioxidant effect. In addition, it was observed that extracts obtained from ethanol and methanol have higher antioxidant capacity than extracts obtained from water.

scholarly journals Flaxseed oil and clove leaf essential oil in Zebrafish diet (Danio rerio)

2020 ◽  
Vol 43 ◽  
pp. e48126
Author(s):  
Thiberio Carvalho da Silva ◽  
Michele Silva ◽  
Fabiana Carbonera ◽  
Jesui Vergilio Visentainer ◽  
Karina Sayuri Utsunomiya ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Capacity ◽  
Danio Rerio ◽  
Interaction Effect ◽  
Growth Rates ◽  
Specific Growth ◽  
Flaxseed Oil ◽  
Leaf Essential Oil ◽  
Specific Growth Rates

Flaxseed oil is recognized as the plant source richest in α-linolenic acid, whereas clove leaf essential oil has a strong antioxidant capacity. The objective of this study was to determine the in vitro antioxidant capacity of diets containing a combination of flaxseed oil (FO) and clove leaf essential oil (CLEO), as well as to use zebrafish (Danio rerio) to assess their effect on the animals’ growth. Fifty days after hatching, a total of 420 male specimens (0.29 ± 0.04 g) were divided into seven groups for each diet and fed for 55 days to be used, with the diets being: control, absent FO and CLEO; 3% FO + 0.5% CLEO; 3% FO + 1% CLEO; 6% FO + 0.5% CLEO; 6% FO + 1% CLEO; 9% FO + 0.5% CLEO and 9% FO + 1% CLEO. Antioxidant activity was determined through DPPH (2,2-diphenyl-1-picrylhydrazyl) tests, showing interaction effect between factors (FO x CLEO, p < 0.05); the diets containing 1% combined with 3, 6 or 9% of FO presented means higher than those of the 0.5% diets. No mortality was observed during the experiment. For final weight and weight gain, there was no interaction effect (p > 0.05), only isolated effect for FO, with the fish fed 6 and 9% diets having the best results. Final total length and specific growth rates showed interaction effect (p < 0.05). As for specific growth rates, the best response was that of the diet with 6% FO and 0.5% CLEO. Final length showed increase with FO levels, even when there was association with 0.5 or 1% of CLEO. Therefore, combined use of 9% of FL with 0.5% of CLEO is recommended for zebrafish.

scholarly journals Phenolic Composition, Antioxidant Activity, andIn VitroAvailability of Four Different Berries

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Javier Marhuenda ◽  
María Dolores Alemán ◽  
Amadeo Gironés-Vilaplana ◽  
Alfonso Pérez ◽  
Gabriel Caravaca ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic ◽  
Phenolic Composition ◽  
Total Phenolic Compounds ◽  
Anthocyanin Concentration

Polyphenols from berries have proved healthy effects after“in vitro”and“in vivo”studies, such as preventing tumor growing and neurodegenerative and cardiovascular diseases. We compared four different kinds of berries—strawberry, raspberry, blackberry, and blueberry—with the aim to distinguish their phenolic composition, concerning their antioxidant capacity along with their“in vitro”availability. Folin-Ciocalteu method was used for the determination of phenolic compounds, and the antioxidant capacity was measured by ORAC method. Moreover, the determination of anthocyanins was accomplished with an HPLC-DAD. Finally, we carried out an“in vitro”digestion to simulate the gastrointestinal digestion. All berries showed good antioxidant capacity with significant differences, besides high total phenolic compounds. Content of anthocyanins measured by HPLC-DAD varied between the different berries, namely, blackberries and strawberries which showed higher anthocyanin concentration. After“in vitro”digestion, berries showed poor bioavailability of the analysis of anthocyanins (9.9%–31.7%). Availability of total phenolic compounds was higher than anthocyanins (33%–73%). Moreover, strawberries and blackberries presented the less availability grade. Decrease in antioxidant activity measured by ORAC method was about 90% in all berries studied. Therefore, bioavailability of phenolic compounds remains unclear and more correlation between“in vitro”and“in vivo”studies seems to be necessary.

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