The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice

Pulmonary embolism (PE) is the most life-threatening complication of venous thromboembolism, but few effective treatments have been discovered to attenuate chronic PE currently. In this study, we investigated the protective effects of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1) combination (SalB/Rg1) on chronic PE and explored the potential mechanisms. The PE model was induced by 45 μm polystyrene microspheres and 20 mg/kg of SalB/Rg1 was administered to PE rats intraperitoneally. A histopathological analysis of the lungs and heart was performed through hematoxylin and eosin staining and immunohistochemical analysis. The pulmonary index and right ventricular cardiomyocyte cross-sectional area were evaluated. SalB/Rg1 markedly downregulated pulmonary index, attenuated pulmonary interstitial changes, suppressed neutrophil infiltration, prevented collagen deposition, and inhibited MMP-9 activities in the lung. We also found that SalB/Rg1 improved right ventricular hypertrophy accompanied by reducing the cardiomyocyte cross-sectional area. These data suggest that SalB/Rg1 played a protective role against microsphere-induced PE and holds a high potential for the treatment of PE in the future.

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Neurovascular protection of salvianolic acid B and ginsenoside Rg1 combination against acute ischemic stroke in rats

Neuroreport ◽

10.1097/wnr.0000000000001706 ◽

2021 ◽

Vol Publish Ahead of Print ◽

Author(s):

Yao Fu ◽

Rongrong Xing ◽

Linlin Wang ◽

Li Yang ◽

Baohong Jiang

Keyword(s):

Ischemic Stroke ◽

Acute Ischemic Stroke ◽

Salvianolic Acid B ◽

Ginsenoside Rg1 ◽

Salvianolic Acid ◽

Neurovascular Protection

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Ginsenoside Rg1 and ginsenoside Rb1 play different role in myocardial infarction rats while combination with salvianolic acid B

European Journal of Integrative Medicine ◽

10.1016/j.eujim.2014.09.099 ◽

2014 ◽

Vol 6 (6) ◽

pp. 730

Author(s):

Yanping Deng ◽

Tingting Zhang ◽

Fukang Teng ◽

Defang Li ◽

Feng Xu ◽

...

Keyword(s):

Myocardial Infarction ◽

Salvianolic Acid B ◽

Ginsenoside Rg1 ◽

Ginsenoside Rb1 ◽

Salvianolic Acid

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Preparation of Compound Danshen Micropowder Tablets and Comparative Study of Pharmacokinetics with Compound Danshen Extraction Tablets

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1033-1034.315 ◽

2014 ◽

Vol 1033-1034 ◽

pp. 315-319

Author(s):

Peng Yi Hu ◽

Qin Zheng ◽

Ying Ying Gong ◽

Guo Song Zhang ◽

Peng Fei Yue ◽

...

Keyword(s):

Comparative Study ◽

Peak Concentration ◽

Salvianolic Acid B ◽

Tanshinone Iia ◽

Pharmacokinetic Parameters ◽

Intragastric Administration ◽

Ginsenoside Rg1 ◽

Ginsenoside Rb1 ◽

Salvianolic Acid

Compond Danshen micropowder tablets and extraction tablets were firstly prepared respectively. Then the contents of salvianolic acid B, tanshinone IIA, tanshinone I, cryptotanshinone, notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in these two kinds of tablets were determined by HPLC, and the results showed that the contents in micropowder tablets were more than them in extraction tablets. A pharmacokinetic comparative study of micropowder tablets with extraction tablets by intragastric administration was developed. Pharmacokinetic parameters suggested that micropowder tablets in rats in vivo bioavailability degree (AUC), peak concentration (Cmax) were 2.14, 1.64 times than the extraction tablets, respectively, and the Tmax of micropowder tablets was longer.

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Salvianolic Acid B Attenuates Apoptosis of HUVEC Cells Treated with High Glucose or High Fat via Sirt1 Activation

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/9846325 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Jinghui Zhai ◽

Lina Tao ◽

Yueming Zhang ◽

Huan Gao ◽

Xiaoyu Qu ◽

...

Keyword(s):

Oxidative Stress ◽

High Glucose ◽

Molecular Mechanisms ◽

Umbilical Vein ◽

Salvianolic Acid B ◽

Sirtuin 1 ◽

High Fat ◽

Salvianolic Acid ◽

Huvec Cells ◽

Diabetic Cardiopathy

High glucose and high fat are important inducements for the development and progression of diabetic cardiopathy. Salvianolic acid B (SAB), which is the most abundant and bioactive compound in Danshen, attenuates oxidative stress-related disorders, such as cardiovascular diseases, cerebral ischemia, and diabetes. However, the effect of SAB on diabetic cardiopathy is not clear. The aim of study was to investigate the effect and the underlying molecular mechanisms of SAB on diabetic cardiopathy in vitro model. The human umbilical vein endothelial (HUVEC) cells were treated with high glucose (HG, 30 mM) or high fat (palmitic acid, PA, 0.75 mM) in the presence or absence of SAB (100, 200, and 400 mg/L) and incubated for 24 h. We found that HG or PA induced apoptosis of HUVEC cells, while treatment with SAB inhibited the apoptosis. We also found that SAB reversed HG- or PA-induced oxidative stress, apoptosis cell cytokines production, and expression of thioredoxin-interacting protein (TXNIP). Moreover, SAB increased HG- or PA-induced expression of Sirtuin 1 (Sirt1), a nicotinamide adenine dinucleotide- (NAD+-) dependent histone deacetylase. Exposure of HUVEC cells to Ex527 (Sirt1 inhibitor) suppressed the effect of SAB on acetyl-p53 and procaspase-3 expressions. In conclusion, the results suggested that SAB could attenuate HUVEC cells damage treated with HG or PA via Sirt1 and might be a potential therapy agent for the diabetic cardiopathy treatment.

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Intraventricular pressure gradients change during the development of left ventricular hypertrophy: Effect of salvianolic acid B and beta-blocker

Ultrasound ◽

10.1177/1742271x20987584 ◽

2021 ◽

pp. 1742271X2098758

Author(s):

Danfu Ma ◽

Ahmed S Mandour ◽

Tomohiko Yoshida ◽

Katsuhiro Matsuura ◽

Kazumi Shimada ◽

...

Keyword(s):

Blood Pressure ◽

Treatment Group ◽

Left Ventricular Hypertrophy ◽

Pressure Gradient ◽

Ventricular Hypertrophy ◽

Left Ventricular ◽

Salvianolic Acid B ◽

Intraventricular Pressure ◽

Salvianolic Acid ◽

Intraventricular Pressure Gradient

Introduction Intraventricular pressure gradient is regarded as a non-invasive indicator of diastolic function. Salvianolic acid B (Sal-B), a traditional Asian medicine, revealed its usefulness in myocardial infarction models; however, the hemodynamic effect of salvianolic acid B is still unknown. The present study aimed to investigate the intraventricular pressure gradient changes during the development of left ventricular hypertrophy with or without salvianolic acid B and a beta-blocker. Methods In total, 48 rats were divided into four groups; Sham, Non-treatment, salvianolic acid B, and Carvedilol. Aortic coarctation-induced left ventricular hypertrophy was done in three groups and the treatment was started from the third to the sixth week. Blood pressure, conventional echocardiography, and color M-mode echocardiography for measurement of intraventricular pressure gradient were carried out for six consecutive weeks. Results At 4.5 weeks, the LV mass was elevated in the coarctation groups but the blood pressure was significantly lower in salvianolic acid B and Carvedilol groups ( P < 0.05). In the Non-treatment group, the total intraventricular pressure gradient was increased at 4.5 and 6 weeks (2.60 and 2.65, respectively). Meanwhile, the basal intraventricular pressure gradient was elevated at 3 and 6 weeks (1.67 and 1.75) compared with the Sham group. Salvianolic acid B and Carvedilol significantly reduced the basal intraventricular pressure gradient at six weeks compared with the Non-treatment group (1.52 and 1.51 vs 1.75, respectively). Conclusions Salvianolic acid B and Carvedilol promote cardiac function by decreasing the elevated basal intraventricular pressure gradient. The current preclinical results revealed the efficacy of salvianolic acid B as a potential therapy for left ventricular hypertrophy because of the non-blood pressure lowering effect.

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