Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression

Opuntia humifusa is a type of cactus whose fruits have been used in folk medicine for the treatment of several diseases. In the present study, we aimed to determine whether O. humifusa fruit water extract (OHE) has inhibitory effects against solar ultraviolet (sUV)-induced matrix metalloproteinase-1 (MMP-1) expression. In ex vivo human skin, we found that OHE suppressed sUV radiation-induced MMP-1 expression. The inhibitory effect of OHE was confirmed in human dermal fibroblasts. OHE treatment reduced sUV-induced MMP-1 expression by suppressing reactive oxygen species (ROS) generation and phosphorylation of c-Jun, a component of transcription factor activator protein 1 (AP-1). On the other hand, OHE recovered the tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and type 1 collagen production attenuated by sUV. As upstream signaling pathways for AP-1, MKK4-JNK, MEK-ERK, and MKK3/6-p38 phosphorylation were downregulated by OHE treatment. In addition, OHE exhibited DPPH radical scavenging activity. These findings demonstrate that OHE has a preventive effect against sUV-induced skin damage via suppression of pathways triggered by ROS.

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Production of exopolysaccharide from mycelial culture ofGrifola frondosaand its inhibitory effect on matrix metalloproteinase-1 expression in UV-irradiated human dermal fibroblasts

FEMS Microbiology Letters ◽

10.1016/j.femsle.2005.08.021 ◽

2005 ◽

Vol 251 (2) ◽

pp. 347-354 ◽

Cited By ~ 32

Author(s):

Jun Tae Bae ◽

Gwan Sub Sim ◽

Dong Hwan Lee ◽

Bum Chun Lee ◽

Hyeong Bae Pyo ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Inhibitory Effect ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Mycelial Culture ◽

Matrix Metalloproteinase 1

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Inhibitory effect ofAstragalus membranaceusroot on matrix metalloproteinase-1 collagenase expression and procollagen destruction in ultraviolet B-irradiated human dermal fibroblasts by suppressing nuclear factor kappa-B activity

Journal of Pharmacy and Pharmacology ◽

10.1111/j.2042-7158.2012.01570.x ◽

2012 ◽

Vol 65 (1) ◽

pp. 142-148 ◽

Cited By ~ 21

Author(s):

Min Jung Hong ◽

Eun Bit Ko ◽

Seong Kyu Park ◽

Mun Seog Chang

Keyword(s):

Matrix Metalloproteinase ◽

Nuclear Factor Kappa B ◽

Inhibitory Effect ◽

Dermal Fibroblasts ◽

Ultraviolet B ◽

Nuclear Factor ◽

Human Dermal Fibroblasts ◽

Nuclear Factor Kappa ◽

Matrix Metalloproteinase 1

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Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation

Antioxidants ◽

10.3390/antiox9100912 ◽

2020 ◽

Vol 9 (10) ◽

pp. 912

Author(s):

Mirae An ◽

Hyungkeun Kim ◽

Joo-Myung Moon ◽

Hyun-Soo Ko ◽

Paul Clayton ◽

...

Keyword(s):

Cathepsin B ◽

Hydrolytic Enzyme ◽

Reactive Oxygen Species Generation ◽

Dermal Fibroblasts ◽

Ros Generation ◽

Skin Damage ◽

Zizania Latifolia ◽

Expression Levels ◽

Membrane Rupture ◽

Uva Irradiation

Ultraviolet A (UVA) is a risk factor for photoaging and wrinkle formation. Zizania latifolia is an herbaceous perennial plant. It contains many bioactive compounds such as tricin that show antioxidative and anti-inflammatory effects. The aim of this study was to investigate the antiwrinkle effect of a mixture of hydrolytic enzyme (cellulase, hemicellulase and pectinase)-treated Z. latifolia extract (ZLE) and tricin on UVA-irradiated human dermal fibroblasts (HDFs) and SKH-1 hairless mice. Treatment of UVA-irradiated HDF cells with ZLE and tricin significantly decreased UVA induced-plasma membrane rupture, generation of ROS, expression levels of total and secreted lysosomal associated membrane protein (LAMP-1), cathepsin B and metalloproteinases (MMPs) and inhibited NF-κB activation. In the animal study, UVA-damaged epidermal and dermal tissues were repaired by the ZLE and tricin treatments. Administration of ZLE or tricin to UVA-irradiated animals recovered skin surface moisture and collagen fiber in dermal tissue. Treatment of ZLE or tricin decreased wrinkle formation, secretion of MMPs and expression levels of vascular endothelial growth factor (VEGF) and cathepsin B, and increased the expression level of collagen-1 in UVA-irradiated animals. Overall, the ZLE and tricin treatments decreased the skin damage induced by UVA irradiation via inhibition of lysosomal exocytosis and ROS generation. Therefore, ZLE and tricin are promising as antiwrinkle and antiphotoaging agents.

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