Inhibitory effect ofAralia elataethanol extract against skin damage in UVB-exposed human keratinocytes and human dermal fibroblasts

Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) level and improved the viability of UVB-irradiated HDF cells in a dose-dependent manner. Furthermore, HFPS significantly inhibited intracellular collagenase and elastase activities, remarkably protected collagen synthesis, and reduced matrix metalloproteinases (MMPs) expression by regulating nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in UVB-irradiated HDF cells. These results suggest that HFPS possesses strong UV protective effect, and can be a potential ingredient in the pharmaceutical and cosmetic industries.

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Inhibitory Effect of Opuntia humifusa Fruit Water Extract on Solar Ultraviolet-Induced MMP-1 Expression

International Journal of Molecular Sciences ◽

10.3390/ijms19092503 ◽

2018 ◽

Vol 19 (9) ◽

pp. 2503 ◽

Cited By ~ 4

Author(s):

Ah-Ram Han ◽

Tae-Gyu Lim ◽

Young-Ran Song ◽

Mi Jang ◽

Young Rhee ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Radical Scavenging ◽

Water Extract ◽

Inhibitory Effect ◽

Dermal Fibroblasts ◽

Ros Generation ◽

Skin Damage ◽

Opuntia Humifusa ◽

Matrix Metalloproteinase 1 ◽

Solar Ultraviolet

Opuntia humifusa is a type of cactus whose fruits have been used in folk medicine for the treatment of several diseases. In the present study, we aimed to determine whether O. humifusa fruit water extract (OHE) has inhibitory effects against solar ultraviolet (sUV)-induced matrix metalloproteinase-1 (MMP-1) expression. In ex vivo human skin, we found that OHE suppressed sUV radiation-induced MMP-1 expression. The inhibitory effect of OHE was confirmed in human dermal fibroblasts. OHE treatment reduced sUV-induced MMP-1 expression by suppressing reactive oxygen species (ROS) generation and phosphorylation of c-Jun, a component of transcription factor activator protein 1 (AP-1). On the other hand, OHE recovered the tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and type 1 collagen production attenuated by sUV. As upstream signaling pathways for AP-1, MKK4-JNK, MEK-ERK, and MKK3/6-p38 phosphorylation were downregulated by OHE treatment. In addition, OHE exhibited DPPH radical scavenging activity. These findings demonstrate that OHE has a preventive effect against sUV-induced skin damage via suppression of pathways triggered by ROS.

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Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro

International Journal of Molecular Sciences ◽

10.3390/ijms21207756 ◽

2020 ◽

Vol 21 (20) ◽

pp. 7756

Author(s):

Jung Hwan Oh ◽

Fatih Karadeniz ◽

Chang-Suk Kong ◽

Youngwan Seo

Keyword(s):

Molecular Mechanisms ◽

Type I Collagen ◽

Nuclear Translocation ◽

Quinic Acid ◽

Dermal Fibroblasts ◽

Type I ◽

Human Dermal Fibroblasts ◽

Skin Damage ◽

Chronological Aging

Cutaneous aging is divided into intrinsic and exogenous aging correspondingly contributing to the complex biological phenomenon in skin. Intrinsic aging is also termed chronological aging, which is the accumulation of inevitable changes over time and is largely genetically determined. Superimposed on this intrinsic process, exogenous aging is associated with environmental exposure, mainly to ultraviolet (UV) radiation and more commonly termed as photoaging. UV-induced skin aging induces increased expression of matrix metalloproteinases (MMPs) which in turn causes the collagen degradation. Therefore, MMP inhibitors of natural origin are regarded as a primary approach to prevent or treat photoaging. This study investigated the effects of 3,5-dicaffeoyl-epi-quinic acid (DEQA) on photoaging and elucidated its molecular mechanisms in UVA-irradiated human dermal fibroblasts (HDFs). The results show that treatment with DEQA decreases MMP-1 production and increases type I collagen production in UVA-damaged HDFs. In addition, treatment of UVA-irradiated HDFs with DEQA downregulates MMP-1, MMP-3 and MMP-9 expression via blocking MAPK-cascade-regulated AP-1 transcriptional activity in UVA-irradiated HDFs. Furthermore, DEQA relieves the UVA-mediated suppression of type I procollagen and collagen expression through stimulating TGF-β/Smad signaling, leading to activation of the Smad 2/3 and Smad 4 nuclear translocation. These results suggest that DEQA could be a potential cosmetic agent for prevention and treatment of skin photoaging.

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Inhibitory effect of vitamin C on intrinsic aging in human dermal fibroblasts and hairless mice

Food Science and Biotechnology ◽

10.1007/s10068-017-0252-6 ◽

2017 ◽

Cited By ~ 2

Author(s):

Jae-Hong Jeong ◽

Mi-Bo Kim ◽

Changhee Kim ◽

Jae-Kwan Hwang

Keyword(s):

Vitamin C ◽

Inhibitory Effect ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Hairless Mice

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Adherence and viability of primary human keratinocytes and primary human dermal fibroblasts on acrylonitrile-based copolymers with different concentrations of positively charged functional groups

Clinical Hemorheology and Microcirculation ◽

10.3233/ch-2012-1613 ◽

2012 ◽

Vol 52 (2-4) ◽

pp. 391-401 ◽

Cited By ~ 5

Author(s):

Karoline Trescher ◽

Nico Scharnagl ◽

Karl Kratz ◽

Toralf Roch ◽

Andreas Lendlein ◽

...

Keyword(s):

Functional Groups ◽

Human Keratinocytes ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Primary Human Keratinocytes ◽

Positively Charged

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Inhibitory Effect of Tetrandrine on the Proliferation of Human Dermal Fibroblasts Derived from Hypertrophic Scars

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.268-270.838 ◽

2011 ◽

Vol 268-270 ◽

pp. 838-840

Author(s):

De Wu Liu ◽

Xiang Hu ◽

De Ming Liu ◽

Ping Zou

Keyword(s):

Hypertrophic Scar ◽

Culture Medium ◽

Inhibitory Effect ◽

Dermal Fibroblasts ◽

Dependent Manner ◽

Human Dermal Fibroblasts ◽

Hypertrophic Scars ◽

Result Show ◽

Dose Dependent

Tetrandrine can inhibit the proliferation and collagen synthesis of fibroblasts in lung and liver tissue confirmed by a series of clinical research. In this chapter, we investigated the effect of Tetrandrine on the proliferation of human dermal fibroblasts derived from hypertrophic scars. The dermal fibroblasts were isolated from human hypertrophic scar tissues and cultured in vitro. Tetrandrine with different concentration were added to culture medium respectively. The proliferative activities were determined. The result show that when the concentration of added Tetrandrine increased from 5μg/ml to 80μg/ml, the proliferative activities of cultured dermal fibroblasts were decreased gradually in dose-dependent manner. It conclusions that Tetrandrine can obviously inhibit the proliferation of human dermal fibroblasts derived from hypertrophic scars.

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In Vitro and In Vivo Photoprotective Effects of (-)-Loliode Isolated from the Brown Seaweed, Sargassum horneri

Molecules ◽

10.3390/molecules26226898 ◽

2021 ◽

Vol 26 (22) ◽

pp. 6898

Author(s):

Lei Wang ◽

Hyun-Soo Kim ◽

Jun-Geon Je ◽

Xiaoting Fu ◽

Caoxing Huang ◽

...

Keyword(s):

Brown Seaweed ◽

Human Keratinocytes ◽

Dermal Fibroblasts ◽

Sargassum Horneri ◽

Skin Damage ◽

In Vivo Models ◽

In Vivo Test ◽

Skin Cells

Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed Sargassum horneri, showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.

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