scholarly journals Gummy Stem Blight Resistance in Melon: Inheritance Pattern and Development of Molecular Markers

2018 ◽  
Vol 19 (10) ◽  
pp. 2914 ◽  
Author(s):  
Md Zahid Hassan ◽  
Md Abdur Rahim ◽  
Sathishkumar Natarajan ◽  
Arif Hasan Khan Robin ◽  
Hoy-Taek Kim ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Recessive Gene ◽  
Chromosome 9 ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
F2 Population ◽  
First Intron ◽  
Frameshift Deletion ◽  
Cucumis Melo L ◽  
Selection Of

Gummy stem blight (GSB) causes enormous losses to melon (Cucumis melo L.) production worldwide. We aimed to develop useful molecular markers linked to GSB resistance. In this study, 168 F2 plants were obtained from the F1 population of a cross between the GSB-susceptible ‘Cornell ZPPM 339’ and the GSB-resistant ‘PI482399’ lines. A 3:1 ratio of susceptible and resistant genotypes was observed in the F2 population, indicating control by a single recessive gene. Nucleotide-binding site leucine-rich repeat (NBS-LRR) genes confer resistance against insects and diseases in cucurbits including melon. We cloned and sequenced the TIR-NBS-LRR-type resistance gene MELO3C022157, located on melon chromosome 9, from resistant and susceptible lines. Sequence analysis revealed deletions in the first intron, a 2-bp frameshift deletion from the second exon and a 7-bp insertion in the 4th exon of the resistant line. We developed two insertion/deletion (InDel) markers, GSB9-kh-1 and GSB9-kh-2, which were found in the first intron of MELO3C022157 linked to GSB resistance. We validated these markers with the F2 population and inbred lines. These InDels may be used to facilitate marker-assisted selection of GSB resistance in melon. However, functional analysis of overexpressing and/or knock-down mutants is needed to confirm the frameshift mutation.

scholarly journals QTL Mapping for Gummy Stem Blight Resistance in Watermelon (Citrullus spp.)

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 500
Author(s):  
Eun Su Lee ◽  
Do-Sun Kim ◽  
Sang Gyu Kim ◽  
Yun-Chan Huh ◽  
Chang-Gi Back ◽  
...  
Keyword(s):  
Chromosome 8 ◽  
Chromosome 6 ◽  
Potential Candidate ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
F2 Population ◽  
Leaf Lesion ◽  
Phenotypic Variations ◽  
A Minor ◽  
Selection Of

Watermelon (Citrulluslanatus) is an economically important fruit crop worldwide. Gummy stem blight (GSB) is one of the most damaging diseases encountered during watermelon cultivation. In the present study, we identified quantitative trait loci (QTLs) associated with GSB resistance in an F2 population derived from a cross between maternal-susceptible line ‘920533’ (C. lanatus) and the paternal-resistant line ‘PI 189225’ (C. amarus). The resistance of 178 F2 plants was assessed by two different evaluation methods, including leaf lesion (LL) and stem blight (SB). To analyze the QTLs associated with GSB resistance, a linkage map was constructed covering a total genetic distance of 1070.2 cM. QTL analysis detected three QTLs associated with GSB resistance on chromosome 8 and 6. Among them, two QTLs, qLL8.1 and qSB8.1 on chromosome 8 identified as major QTLs, explaining 10.5 and 10.0% of the phenotypic variations localizing at same area and sharing the same top markers for both LL and SB traits, respectively. A minor QTL, qSB6.1, explains 9.7% of phenotypic variations detected on chromosome 6 only for the SB trait. High-throughput markers were developed and validated for the selection of resistant QTLs using watermelon accessions, and commercial cultivars. Four potential candidate genes were predicted associated with GSB resistance based on the physical location of flanking markers on chromosome 8. These findings will be helpful for the development of watermelon cultivars resistant to GSB.

scholarly journals Deteksi Gen Ketahanan Terhadap Gsb-4 (Gummy Stem Blight) pada Tanaman Melon (Cucumis Melo L.)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Ganies Riza Aristya Aisha Rizky Rahmawati Budi Setiadi Daryono
Keyword(s):  
Simple Sequence Repeat ◽  
Cucumis Melo ◽  
Sequence Repeat ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Cucumis Melo L ◽  
Simple Sequence

Perakitan melon tahan penyakit gummy stem blight (Gsb-4) merupakan upaya yang dapat dilakukan untuk menanggulangi penyakit gummy stem blight. Untuk itu harus diketahui ada tidaknya gen ketahanan terhadap gummy stem blight. Tujuan penelitian ini adalah mendeteksi adanya gen ketahanan terhadap gummy stem blight pada 18 kultivar melon dan 1 kultivar mentimun. Hasil amplifikasi menggunakan penanda molekular Simple Sequence Repeat (SSR) dengan primer spesifik yaitu CMTA170a menunjukkan pita berukuran 120 bp sedangkan dengan primer CMCT160a+b menunjukkan pita berukuran 212 bp. Hasil yang diuji menunjukkan bahwa semua kultivar melon memiliki gen ketahanan terhadap gummy stem blight baik homozigot maupun heterozigot.

scholarly journals Identification of Amplified Fragment Length Polymorphism Markers Linked to Gummy Stem Blight (Didymella bryoniae) Resistance in Melon (Cucumis melo L.) PI 420145

HortScience ◽  
2009 ◽  
Vol 44 (1) ◽  
pp. 32-34 ◽  
Author(s):  
Joseph N. Wolukau ◽  
Xiaohui Zhou ◽  
JinFeng Chen
Keyword(s):  
Molecular Markers ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Dominant Gene ◽  
Amplified Fragment Length Polymorphism ◽  
Aflp Markers ◽  
Didymella Bryoniae ◽  
Stem Blight ◽  
Gummy Stem Blight

Gummy stem blight (GSB) caused by the ascomycete fungus Didymella bryoniae (Auersw.) Rehm is an important disease of melon. Molecular markers linked to resistance would be useful for melon breeding programs. The amplified fragment length polymorphism (AFLP) technique and bulk segregant analysis were used to identify molecular markers linked to the resistance of melon to Didymella bryoniae. Segregation analysis of F2 progeny from a cross of PI 420145, a resistant line, and PI 136170, a susceptible line, showed that resistance to GSB was controlled by a dominant gene. One AFLP marker, E-TG/M-CTC200, was identified that is tightly linked to GSB resistance gene at a distance of 2.0 cM. To our best knowledge, this is the first report of AFLP markers linked to GSB resistance in melon. The identification of AFLP markers provides a step toward the use of marker-assisted selection and the characterization of the gene encoding resistance to GSB in melon.

scholarly journals Selection of melon genotypes with resistance to Didymella bryoniae using a diallel approach

2019 ◽  
Vol 41 (1) ◽  
pp. 42600
Author(s):  
Lucas Da Silva Santos ◽  
Guilherme Matos Martins Diniz ◽  
Rafaelle Fazzi Gomes ◽  
Edgard Henrique Costa Silva ◽  
Alysson Jalles da Silva ◽  
...  
Keyword(s):  
Rating Scale ◽  
Didymella Bryoniae ◽  
Reciprocal Effect ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Group I ◽  
Diallel Mating Design ◽  
Resistant Lines ◽  
Selection Of ◽  
Partial Diallel

The development of melon lines resistant to gummy stem blight (GSB) is an important strategy for decreasing losses caused by this disease. Thus, selecting the best parents for such a goal is essential. We evaluated the general (GCA) and specific (SCA) combining abilities of lines and accessions to guide the selection of the most promising parents to develop GSB-resistant lines. Six genotypes representing two groups (group I - susceptible: group II - resistant) were crossed in a partial diallel mating design. The genotypes were evaluated for disease expression using a rating scale from 0 (resistant) to 4 (susceptible). Estimates of GCA were negative and significant for the accessions PI 420145 and PI 482398, which can be suggested for crosses aimed at obtaining melon lines resistant to GSB. There is a reciprocal effect for the resistance of melon genotypes to Didymella bryoniae, especially when PI 482398, PI 420145, and PI 140471 are crossed with JAB-20. Such results indicate that maternal effects may be involved in the resistance to gummy stem blight.

scholarly journals Identification of Quantitative Trait Loci Conferring Resistance to Bemisia tabaci in an F2 Population of Solanum lycopersicum × Solanum habrochaites Accession LA1777

2010 ◽  
Vol 135 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Aliya Momotaz ◽  
Jay W. Scott ◽  
David J. Schuster
Keyword(s):  
Molecular Markers ◽  
Solanum Lycopersicum ◽  
Bemisia Tabaci ◽  
Quantitative Trait ◽  
Fixed Effects ◽  
Chromosome 9 ◽  
Chromosome 11 ◽  
Chi Square ◽  
Solanum Habrochaites ◽  
F2 Population

Solanum habrochaites S. Knapp and D.M. Spooner accession LA1777 have reported resistance to the sweetpotato whitefly (SPWF), Bemisia tabaci (Genn.). An interspecific F2 population of 171 plants between tomato [Solanum lycopersicum L. (formerly Lycopersicon esculentum Mill.)] and LA1777 was bioassayed against adult SPWF in a greenhouse using clip cages. A selective genotyping analysis was used with 11 resistant and 10 susceptible plants to locate resistance genes by testing them with molecular markers spanning most of the tomato genome at about 10-cM intervals. Markers in four regions were found to be associated with resistance, where three of them showed significantly strong associations and one showed a weak association through chi-square and analyses of variance. However, through quantitative trait locus (QTL) analysis using molecular markers, all four regions were identified as major QTLs with logarithm of odds (LOD) values of 4.87 to 5.95. The four QTLs were identified near the markers TG313 on chromosome 10, C2_At2g41680 on chromosome 9, TG523/T0408 on chromosome 11, and TG400/cLEG-37-G17 on chromosome 11. Multiple regression analysis produced similar results as above with fixed effects of single loci as well as interaction among some of the QTLs.

scholarly journals QTL associated with gummy stem blight resistance in watermelon

2020 ◽  
Author(s):  
Winnie Gimode ◽  
Kan Bao ◽  
Zhangjun Fei ◽  
Cecilia McGregor
Keyword(s):  
Disease Resistance ◽  
Phenotypic Variation ◽  
Citrullus Lanatus ◽  
Genetic Resistance ◽  
Blight Resistance ◽  
Yield Losses ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Cost Of Disease ◽  
Selection Of

Abstract Key message We identified QTLs associated with gummy stem blight resistance in an interspecific F2:3Citrullus population and developed marker assays for selection of the loci in watermelon. Abstract Gummy stem blight (GSB), caused by three Stagonosporopsis spp., is a devastating fungal disease of watermelon (Citrullus lanatus) and other cucurbits that can lead to severe yield losses. Currently, no commercial cultivars with genetic resistance to GSB in the field have been reported. Utilizing GSB-resistant cultivars would reduce yield losses, decrease the high cost of disease control, and diminish hazards resulting from frequent fungicide application. The objective of this study was to identify quantitative trait loci (QTLs) associated with GSB resistance in an F2:3 interspecific Citrullus mapping population (N = 178), derived from a cross between Crimson Sweet (C. lanatus) and GSB-resistant PI 482276 (C. amarus). The population was phenotyped by inoculating seedlings with Stagonosporopsis citrulli 12178A in the greenhouse in two separate experiments, each with three replications. We identified three QTLs (ClGSB3.1, ClGSB5.1 and ClGSB7.1) associated with GSB resistance, explaining between 6.4 and 21.1% of the phenotypic variation. The genes underlying ClGSB5.1 includes an NBS-LRR gene (ClCG05G019540) previously identified as a candidate gene for GSB resistance in watermelon. Locus ClGSB7.1 accounted for the highest phenotypic variation and harbors twenty-two candidate genes associated with disease resistance. Among them is ClCG07G013230, encoding an Avr9/Cf-9 rapidly elicited disease resistance protein, which contains a non-synonymous point mutation in the DUF761 domain that was significantly associated with GSB resistance. High throughput markers were developed for selection of ClGSB5.1 and ClGSB7.1. Our findings will facilitate the use of molecular markers for efficient introgression of the resistance loci and development of GSB-resistant watermelon cultivars.

scholarly journals Monogenic Dominant Resistance to Gummy Stem Blight in Two Melon (Cucumis melo) Accessions

Plant Disease ◽  
1999 ◽  
Vol 83 (12) ◽  
pp. 1105-1107 ◽  
Author(s):  
T. L. Zuniga ◽  
J. P. Jantz ◽  
T. A. Zitter ◽  
M. K. Jahn
Keyword(s):  
Cucumis Melo ◽  
Dominant Gene ◽  
Plant Introduction ◽  
Didymella Bryoniae ◽  
Susceptible Parent ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Backcross Populations ◽  
Relationship Of ◽  
Cucumis Melo L

Two melon (Cucumis melo L.) accessions, plant introduction (PI) 157082 and PI 511890, reported to be resistant to gummy stem blight, a disease incited by the fungus Didymella bryoniae, were crossed with a susceptible parent to determine the inheritance of resistance. Resistance in both accessions is due to a single dominant gene, based on analysis of F1, F2, and backcross populations. Additionally, PI 157082 was crossed with PI 140471, the other source of resistance identified to date, to examine the genetic relationship of resistance found in these two sources. The frequency of susceptible individuals from the (PI 157082 × 140471) F2 population was consistent with a 15:1 resistant:susceptible ratio, indicating that PIs 140471 and 157082 possess different resistance genes.

Molecular Tagging of Gummy Stem Blight Resistance in Watermelon

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 472e-472
Author(s):  
Young-Seok Kwon ◽  
Young Hyun Om ◽  
Leigh Hawkins ◽  
Fenny Dane
Keyword(s):  
Plant Introduction ◽  
Aflp Markers ◽  
Blight Resistance ◽  
Didymella Bryoniae ◽  
Molecular Tagging ◽  
Stem Blight ◽  
Gummy Stem Blight ◽  
Blight Disease ◽  
Fungus Disease ◽  
Selection Of

The fungus Didymella bryoniae causes gummy stem blight (foliar phase) and black rot (fruit phase) in cucurbits. As resistance to other diseases have been incorporated into cucurbits, losses due to gummy stem blight have increased. Populations derived from crosses between a cultivated watermelon (SS-4), susceptible to the fungus Didymella bryoniae and resistant plant introduction accessions (PI 189225 and PI 272778) are used to detect molecular markers linked to gummy stem blight disease resistance. Genomic DNA was purified from primary leaves of watermelon seedlings before seedlings were inoculated with a spore suspension of the fungus. Disease severity was rated 4 days after inoculation. DNA bulked from resistant or susceptible BC seedlings was amplified to detect AFLP markers. Linkages between gummy stem blight resistance and AFLP markers were identified and should facilitate evaluation and selection of watermelon populations segregating for gummy stem blight resistance.

Factors Promoting Pycnidia Production of Didymella bryoniae, the Causal of Gummy Stem Blight in Cucurbits

2017 ◽  
Vol 45 (1) ◽  
pp. 173-187
Author(s):  
Abdel-Fattah El-Wakil ◽  
Amal Khalil ◽  
Ibrahim El -Abbasi
Keyword(s):  
Didymella Bryoniae ◽  
Stem Blight ◽  
Gummy Stem Blight
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