scholarly journals Improvement of Soybean Agrobacterium-Mediated Transformation Efficiency by Adding Glutamine and Asparagine into the Culture Media

2018 ◽  
Vol 19 (10) ◽  
pp. 3039 ◽  
Author(s):  
Li Chen ◽  
Yupeng Cai ◽  
Xiujie Liu ◽  
Weiwei Yao ◽  
Chen Guo ◽  
...  
Keyword(s):  
Culture Media ◽  
Genetically Modified ◽  
Global Scale ◽  
Transformation Frequency ◽  
Genetically Modified Soybean ◽  
Soybean Transformation ◽  
Agrobacterium Mediated Transformation ◽  
Key Factor ◽  
Probable Role ◽  
Better Than

As a genetically modified crop, transgenic soybean occupies the largest global scale with its food, nutritional, industrial, and pharmaceutical uses.Efficient transformation is a key factor for the improvement of genetically modified soybean. At present, the Agrobacterium-mediated method is primarily used for soybean transformation, but the efficiency of this method is still relatively low (below 5%) compared with rice (above 90%). In this study, we examined the influence of l-glutamine and/or l-asparagine on Agrobacterium-mediated transformation in soybean and explored the probable role in the process of Agrobacterium-mediated transformation. The results showed that when the amino acids l-glutamine and l-asparagine were added separately or together to the culture medium, the shoot induction frequency, elongation rate, and transformation frequency were improved. The combined effects of l-glutamine and l-asparagine were better than those of l-glutamine and l-asparagine alone. The 50 mg/L l-glutamine and 50 mg/L l-asparagine together can enhance the transformation frequency of soybean by attenuating the expression level of GmPRs (GmPR1, GmPR4, GmPR5, and GmPR10) and suppression of the plant defense response. The transgene was successfully transmitted to the T1 generation. This study will be useful in genetic engineering of soybean.

Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean

2007 ◽  
Vol 42 (10) ◽  
pp. 1249-1255 ◽  
Author(s):  
Cibele dos Santos Ferrari ◽  
Luciana Lehmkuhl Valente ◽  
Fábio Cristiano Angonesi Brod ◽  
Caroline Tagliari ◽  
Ernani Sebastião Sant'Anna ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Isolation ◽  
Genetically Modified ◽  
Chain Reaction ◽  
Genetically Modified Soybean ◽  
Polymerase Chain

Influences of Duty Ratio on Structure and Corrosion Resistance of Ceramic Coatings on Ti-6Al-4V by Micro-Plasma Oxidation

2007 ◽  
Vol 336-338 ◽  
pp. 2454-2457
Author(s):  
Hong Qi Ben ◽  
Zhong Ping Yao ◽  
Zhao Hua Jiang
Keyword(s):  
Corrosion Resistance ◽  
Outer Layer ◽  
Oxidation Process ◽  
Ceramic Coatings ◽  
Duty Ratio ◽  
Plasma Oxidation ◽  
Rutile Tio2 ◽  
Key Factor ◽  
Α Al2o3 ◽  
Better Than

Duty ratio is a key factor during micro-plasma oxidation process. Its influences on structure and corrosion resistance of ceramic coatings on Ti–6Al–4V by pulsed bi-polar MPO in NaAlO2 solution were investigated. The ceramic coatings were composed of Al2TiO5, α-Al2O3 and rutile TiO2, of which Al2TiO5 was the main crystalline. The coating can be divided into a porous outer layer and a dense inner layer. Compared with the condition of the same duty ratio for both pulses, the rise of anode duty ratio (D1) led to an increase in the amount of Al2TiO5 and α-Al2O3 and increased the thickness of the coating; whereas the rise of cathode duty ratio (D2) led to a decrease in the amount of α-Al2O3 and decreased the thickness of the coating. Besides, the corrosion resistance of the coated samples was better than that of Ti-6Al-4V substrate. When D1 / D2 = 60 / 30, the produced coating’s corrosion resistance was best.

Monitoring of genetically modified soybean events in sausage products in South Korea

Food Control ◽  
2016 ◽  
Vol 67 ◽  
pp. 63-67 ◽  
Author(s):  
Jae-Hwan Kim ◽  
Ji-Yeon Song ◽  
Yewon Hong ◽  
Hae-Yeong Kim
Keyword(s):  
South Korea ◽  
Genetically Modified ◽  
Genetically Modified Soybean

Coloring genetically modified soybean grains with anthocyanins by suppression of the proanthocyanidin genes ANR1 and ANR2

2011 ◽  
Vol 21 (4) ◽  
pp. 757-771 ◽  
Author(s):  
Nik Kovinich ◽  
Ammar Saleem ◽  
Tara L. Rintoul ◽  
Daniel C. W. Brown ◽  
John T. Arnason ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Genetically Modified Soybean

Croissance et fructification de Ceratocystis spp. avec attention particulière à C. piceae et C. fimbriata, en fonction du substrat

1982 ◽  
Vol 60 (4) ◽  
pp. 358-363
Author(s):  
A. Thuillier ◽  
P. Neumann
Keyword(s):  
Nutrient Solution ◽  
Culture Media ◽  
Basal Medium ◽  
Malt Agar ◽  
Fruiting Bodies ◽  
Ceratocystis Fimbriata ◽  
Good Production ◽  
Wood Extracts ◽  
Standard Culture ◽  
Better Than

Ceratocystis coerulescens, C. fimbriata, C. ips, and C. minor were tested for production of sexual fruiting bodies, and C. penicillata and C. piceae for asexual fruiting bodies. Ceratocystis fimbriata produced perithecia easily on standard culture media, but there were marked differences between the two strains tested (503, 560). Strain 503 had a good production of fruiting bodies on malt agar (M) and a basal nutrient solution (N). Strain 560 fared better than 503 on Leonian agar (L), but did not fructify on M and N. Supplementing media with various wood extracts produced better results. M + maple sapwood extracts and L + poplar sapwood extracts gave the best results with strain 503, and L + pine sapwood extracts was the best with strain 560.Production of coremia was also influenced by the basal medium and the kind of extracts added as supplements. Fir and maple extracts stimulated the production of fruiting bodies, whereas pine and poplar extracts had no or very little stimulating effects. In every other species tested, the production of fruiting bodies was none or very irregular. [Journal translation]

scholarly journals Identification of geneticaly modified soybean seeds resistant to glyphosate

2004 ◽  
Vol 61 (3) ◽  
pp. 336-341 ◽  
Author(s):  
Maria Ângela André Tillmann ◽  
Shirlie West
Keyword(s):  
Genetically Modified ◽  
Soybean Seeds ◽  
Germination Test ◽  
Hypocotyl Length ◽  
Genetically Modified Soybean ◽  
Paper Towel ◽  
Soybean Genotypes ◽  
Physiological Quality ◽  
Resistance Trait ◽  
Moist Paper Towel

Advances in genetic engineering permit the modification of plants to be tolerant to certain herbicides that are usually not selective. For practical and commercial purposes, it is important to be able to detect the presence or absence of these traits in genotypes. The objective of this research was to develop a procedure for identifying genetically modified soybean (Glycine max L. Merr.) with resistance to the herbicide glyphosate. Two studies were conducted based on germination test. In the first study, soybean seeds were pre-imbibed in paper towel with the herbicide solutions, then transferred to moist paper towel for the germination test. In the second study, seeds were placed directly in herbicide solutions in plastic cups and tested for germination using the paper towel method. Eight soybean genotypes were compared: four Roundup Ready, that contained the gene resistant to the herbicide (G99-G725, Prichard RR, G99-G6682, and H7242 RR) and four non-transgenic parental cultivars (Boggs, Haskell, Benning, and Prichard). In the first study, the seeds were imbibed for 16 hours at 25°C in herbicide concentrations between 0.0 and 1.5% of the glyphosate active ingredient. In the second, seeds were subjected to concentrations between 0.0 and 0.48%, for one hour, at 30°C. The evaluation parameters were: germination, hypocotyl length, root length and total length of the seedlings. Both methods are efficient in identifying glyphosate-resistant soybean genotypes. It is possible to identify the genetically modified soybean genotypes after three days, by imbibing the seed in 0.12% herbicide solution, and after six days if the substrate is pre-imbibed in a 0.6% herbicide solution. The resistance trait was identified in all cultivars, independent of the initial physiological quality of the seed.

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