scholarly journals Modern Concepts in Regenerative Therapy for Ischemic Stroke: From Stem Cells for Promoting Angiogenesis to 3D-Bioprinted Scaffolds Customized via Carotid Shear Stress Analysis

2019 ◽  
Vol 20 (10) ◽  
pp. 2574 ◽  
Author(s):  
Annabella Benedek ◽  
Daniel Cernica ◽  
Andras Mester ◽  
Diana Opincariu ◽  
Roxana Hodas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Shear Stress ◽  
Ischemic Stroke ◽  
Therapeutic Angiogenesis ◽  
Current Status ◽  
Regenerative Therapy ◽  
Therapeutic Approaches ◽  
Ischemic Brain ◽  
Promising Tool ◽  
3D Geometry

Ischemic stroke is associated with a tremendous economic and societal burden, and only a few therapies are currently available for the treatment of this devastating disease. The main therapeutic approaches used nowadays for the treatment of ischemic brain injury aim to achieve reperfusion, neuroprotection and neurorecovery. Therapeutic angiogenesis also seems to represent a promising tool to improve the prognosis of cerebral ischemia. This review aims to present the modern concepts and the current status of regenerative therapy for ischemic stroke and discuss the main results of major clinical trials addressing the effectiveness of stem cell therapy for achieving neuroregeneration in ischemic stroke. At the same time, as a glimpse into the future, this article describes modern concepts for stroke prevention, such as the implantation of bioprinted scaffolds seeded with stem cells, whose 3D geometry is customized according to carotid shear stress.

Migration of neural stem cells to ischemic brain regions in ischemic stroke in rats

2013 ◽  
Vol 552 ◽  
pp. 124-128 ◽  
Author(s):  
Jiong Dai ◽  
Shan-Quan Li ◽  
Yong-Ming Qiu ◽  
Wen-Hao Xiong ◽  
Yu-Hua Yin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ischemic Stroke ◽  
Neural Stem Cells ◽  
Brain Regions ◽  
Ischemic Brain

scholarly journals Periodontal Ligament Stem Cells: Current Status, Concerns, and Future Prospects

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Wenjun Zhu ◽  
Min Liang
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Periodontal Regeneration ◽  
Culture Conditions ◽  
Careful Consideration ◽  
Current Status ◽  
Periodontal Ligament Stem Cells ◽  
Differentiation Potential ◽  
Promising Tool ◽  
Proliferation And Differentiation

Periodontal ligament stem cells (PDLSCs), which reside in the perivascular space of the periodontium, possess characteristics of mesenchymal stem cells and are a promising tool for periodontal regeneration. Recently, great progress has been made in PDLSC transplantation. Investigators are attempting to maximize the proliferation and differentiation potential of PDLSCs by modifying culture conditions and applying growth factors. Nevertheless, problems remain. First, incomparability among different studies must be minimized by establishing standard guidelines for culture and identification of PDLSCs. Notably, attention should be paid to the biological safety of PDLSC transplantation. The present review updates the latest findings regarding PDLSCs and discusses standard criteria for culture and identification of PDLSCs. Finally, the review calls for careful consideration of PDLSC transplantation safety.

scholarly journals The Implementation of Preconditioned Epidermal Neural Crest Stem Cells to Combat Ischemic Stroke. Comment on Othman, F.A.; Tan, S.C. Preconditioning Strategies to Enhance Neural Stem Cell-Based Therapy for Ischemic Stroke. Brain Sci. 2020, 10, 893.

2021 ◽  
Vol 11 (5) ◽  
pp. 653
Author(s):  
Sareh Pandamooz ◽  
Benjamin Jurek ◽  
Mohammad Saied Salehi ◽  
Mandana Mostaghel ◽  
Jaleel A. Miyan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Ischemic Stroke ◽  
Brain Injury ◽  
Cell Therapy ◽  
Ischemic Brain Injury ◽  
Ischemic Brain ◽  
Neural Crest Stem Cells ◽  
Cell Based Therapy ◽  
Brain Sciences

In the recent review published in Brain Sciences, Othman and Tan suggested several preconditioning strategies to improve stem cell therapy after ischemic brain injury [...]

Regenerative Therapies in Neonatology: Clinical Perspectives

2012 ◽  
Vol 224 (04) ◽  
pp. 233-240 ◽  
Author(s):  
L. Gortner ◽  
U. Felderhoff-Müser ◽  
D. Monz ◽  
K. Bieback ◽  
H. Klüter ◽  
...  
Keyword(s):  
Stem Cells ◽  
Clinical Trials ◽  
Stem Cell ◽  
Regenerative Therapy ◽  
Controlled Clinical Trials ◽  
Term Neonates ◽  
Promising Tool ◽  
High Risk Infants ◽  
Regenerative Therapies ◽  
Ischemic Encephalopathy

AbstractRegenerative therapy based on stem cells is applied as standard therapy in pediatric oncology. Furthermore, they are frequently used to treat immunodeficiency disorders of infants. For severe neonatal diseases, e. g. hypoxic-ischemic encephalopathy in term neonates or bronchopulmonary dysplasia in preterm infants, animal models have been established. According to some first preclinical results stem cell administration appears as a promising tool to improve the clinical outcome in high-risk infants. Provided the benefit of regenerative therapies can further be evaluated in appropriate preclinical neonate models, carefully controlled clinical trials to assess the significance of regenerative therapies, such as autologous stem cell administration, are indicated.

Extracellular Vesicles from Adipose-derived Stem Cells Promote Microglia M2 Polarization and Neurological Recovery after Ischemic Stroke

2021 ◽  
Author(s):  
Xiaowen Hu ◽  
Jiaji Pan ◽  
Yongfang Li ◽  
Yixu Jiang ◽  
Haoran Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ischemic Stroke ◽  
Brain Injury ◽  
Extracellular Vesicles ◽  
Brain Atrophy ◽  
Ischemic Brain Injury ◽  
Adipose Derived Stem Cells ◽  
Ischemic Brain ◽  
Microglial Polarization ◽  
Related Proteins

Abstract Background: Adipose-derived stem cells (ADSCs) and their extracellular vesicles (EVs) have therapeutic potential in ischemic brain injury, but the underlying mechanism is poorly understood. The current study aimed to explore the contribution of miRNAs in ADSC-EVs to the treatment of cerebral ischemia. Methods: After the intravenous injection of ADSC-EVs, therapeutic efficacy was evaluated by neurobehavioral tests and brain atrophy volume. The polarization of microglia was assessed by immunostaining and qPCR. We further performed miRNA sequencing of ADSC-EVs and analyzed the relationship between the upregulated miRNAs in ADSC-EVs and microglial polarization-related proteins using Ingenuity Pathway Analysis (IPA). Results: The results showed that ADSC-EVs reduced brain atrophy volume, improved neuromotor and cognitive functions after mouse ischemic stroke. The loss of oligodendrocytes was attenuated after ADSC-EVs injection. The number of blood vessels, as well as newly proliferated endothelial cells in the peri-ischemia area were higher in the ADSC-EVs treated group than that in the PBS group. In addition, ADSC-EVs regulated the polarization of microglia, resulting in increased repair-promoting M2 phenotype and decreased pro-inflammatory M1 phenotype. Finally, STAT1 and PTEN were highlighted as two downstream targets of up-regulated miRNAs in ADSC-EVs among 85 microglia/macrophage polarization related proteins by IPA. The inhibition of STAT1 and PTEN by ADSC-EVs were confirmed in cultured microglia. Conclusions: In summary, ADSC-EVs reduced ischemic brain injury, which was associated with the regulation of microglial polarization. miRNAs in ADSC-EVs contributed to their function in regulating microglial polarization by targeting PTEN and STAT1.

scholarly journals Application of stem cell-derived exosomes in ischemic diseases: opportunity and limitations

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Majid Babaei ◽  
Jafar Rezaie
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Communication ◽  
Therapeutic Angiogenesis ◽  
Beneficial Effects ◽  
In Vivo Experiments ◽  
Promising Tool ◽  
Ischemic Diseases

AbstractIschemic diseases characterized by an insufficient blood flow that leads to a decrease in oxygen and nutrient uptake by cells have emerged as an important contributor to both disability and death worldwide. Up-regulation of angiogenesis may be a key factor for the improvement of ischemic diseases. This article searched articles in PubMed with the following keywords: stem cells, exosomes, angiogenesis, ischemic diseases either alone or in grouping form. The most relevant selected items were stem cell-derived exosomes and ischemic diseases. A growing body of evidence indicates that stem cells produce exosomes, which is the novel emerging approach to cell-to-cell communication and offers a new standpoint on known therapeutic strategies of ischemic diseases. Exosomes transport biological molecules such as many types of proteins, RNAs, DNA fragments, signaling molecules, and lipids between cells. Different stem cells release exosomes representing beneficial effects on ischemic diseases as they promote angiogenesis both in vitro and in vivo experiments. Application of exosomes for therapeutic angiogenesis opened new opportunities in the regenerative medicine, however, some limitations regarding exosomes isolation and application remain concerned. In addition, most of the experiments were conducted in preclinical and therefore translation of these results from bench to bed requires more effort in this field. Exosomes from stem cells are a promising tool for the treatment of ischemic diseases. In addition, translation of pre-clinic results into clinic needs further studies in this field.

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