scholarly journals Global Transcriptomic Analysis of Zebrafish Glucagon Receptor Mutant Reveals Its Regulated Metabolic Network

2020 ◽  
Vol 21 (3) ◽  
pp. 724
Author(s):  
Qi Kang ◽  
Mengyi Hu ◽  
Jianxin Jia ◽  
Xuanxuan Bai ◽  
Chengdong Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Metabolic Network ◽  
Amino Acid Content ◽  
Whole Body ◽  
Total Amino Acid ◽  
Sequencing Analysis ◽  
Cell Hyperplasia ◽  
Glucagon Receptor ◽  
New Information ◽  
Total Amino Acid Content

The glucagon receptor (GCGR) is a G-protein-coupled receptor (GPCR) that mediates the activity of glucagon. Disruption of GCGR results in many metabolic alterations, including increased glucose tolerance, decreased adiposity, hypoglycemia, and pancreatic α-cell hyperplasia. To better understand the global transcriptomic changes resulting from GCGR deficiency, we performed whole-organism RNA sequencing analysis in wild type and gcgr-deficient zebrafish. We found that the expression of 1645 genes changes more than two-fold among mutants. Most of these genes are related to metabolism of carbohydrates, lipids, and amino acids. Genes related to fatty acid β-oxidation, amino acid catabolism, and ureagenesis are often downregulated. Among gcrgr-deficient zebrafish, we experimentally confirmed increases in lipid accumulation in the liver and whole-body glucose uptake, as well as a modest decrease in total amino acid content. These results provide new information about the global metabolic network that GCGR signaling regulates in addition to a better understanding of the receptor’s physiological functions.

scholarly journals AMINO ACID PROFILE OF A BASIDIOMYCETOUS EDIBLE MUSHROOM-LENTINUS SAJOR-CAJU

2017 ◽  
Vol 9 (9) ◽  
pp. 252
Author(s):  
Lata . ◽  
Narender Singh Atri
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Standard Technique ◽  
Edible Mushroom ◽  
Amino Acid Profile ◽  
Total Amino Acid ◽  
Isoleucine Leucine ◽  
Total Amino Acid Content ◽  
Basidiomycetous Mushroom

Objective: In this paper amino acid profile of Lentinus sajor-caju (Fr.) Fr., a basidiomycetous mushroom has been investigated.Methods: During the evaluation 15 amino acids (lysine, aspartic acid, serine, threonine, glutamic acid, cysteine, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine and histidine) were determined from the dried sample of Lentinus sajor-caju by following the standard technique of biochemistry using ion-exchange chromatography.Results: The total amino acid content has been evaluated at 18.82 g/100g. Amongst the evaluated amino acids, exogenous amino acid lysine (6.66 g/100g) is preponderantly present in comparison to all other amino acids. The essential amino acid (EAA) index (44.64%) and biological value (BV=36.93%) has also been determined for the examined sample.Conclusion: Lentinus sajor-caju (Fr.) Fr. is a potential source of quality protein with a substantial proportion of exogenous and endogenous amino acids.

Variations in total amino acid content of peanut meal

1973 ◽  
Vol 50 (12) ◽  
pp. 521-523 ◽  
Author(s):  
C. T. Young ◽  
G. R. Waller ◽  
R. O. Hammons
Keyword(s):  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Peanut Meal ◽  
Total Amino Acid ◽  
Total Amino Acid Content

Effect of type of diet (casein-based or protein-free) and caecotrophy on ileal endogenous nitrogen and amino acid flow in rabbits

2004 ◽  
Vol 79 (2) ◽  
pp. 231-240 ◽  
Author(s):  
A. I. García ◽  
J. C. de Bias ◽  
R. Carabaño
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Total Amino Acid ◽  
Total Amino Acid Content ◽  
Present Trial ◽  
Protein Free Diet ◽  
Ingredient Composition ◽  
Intake Ratio

AbstractTwenty-eight New Zealand White X Californian doe rabbits weighing 3791±141 g were surgically fitted with a glass T-cannula at ileum level. Animals were given ad libitum access to either a casein-based diet (C) or a protein-free diet (PF). The only difference in the ingredient composition of the diets was the substitution of 160g/kg of maize starch by casein in the C diet. Animals were randomly allotted to each experimental diet. Seven rabbits per diet were not allowed to practice caecotrophy. The C diet resulted in higher food dry matter (DM) intake (DMI) (111-5 v. 51-5 g/day), food nitrogen intake (3-12 v. 0-20 g/day), ileal flow of DM (56-0 v. 23-8 g/day) and ileal flow of nitrogen (0-72 v. 0-24 g/day) but lower soft faeces DMI (21-7 v. 41-4 g/day) than the PF diet. A linear relationship between ileal endogenous nitrogen flow and total DMI was found for animals given the C but not for the PF diet. The ingestion ofC diet led to a higher (proportionately 0-41, on average), endogenous ileal amino acid flow than PF diet for all amino acids except for glycine. The ileal flow of glycine was proportionately 0-25 higher in animals given the PF diet than in animals given the C diet. The C diet resulted in higher content of nitrogen, threonine and proline in soft faeces than the PF diet. In contrast, the ingestion of the PF diet led to a higher content of lysine and methionine in soft faeces than the C diet. Within the C diet, animals practising caecotrophy showed higher ileal flow of DM (73-4 v. 56-0 g/day) and phenylalanine (0-834 v. 0-677 g/kg DMI) than animals not practising it. Caecotrophy led to a higher endogenous proportion of some of the most important limiting essential amino acids in rabbits, such as arginine, lysine, phenylalanine and threonine. In rabbits, the use of PF diets has limited practical application in the estimation of nitrogen endogenous losses as the low food DMI and the high soft faecesDMI leads to abnormal food: soft faeces intake ratio. Moreover, due to the important effect of caecotrophy on the amino acid composition of endogenous losses, it is important to correct the total amino acid content of the ileal chyme by the undigested amino acid residue coming from the soft faeces intake. This correction could be made, without the methodological complexity implied, by fitting animals with a wooden collar and thereby avoiding caecotrophy as in the present trial.

The main amino acids in xylem sap of Vitis vinifera L. var. Cabernet Franc during the vegetative cycle. Search for an amino acid marker

OENO One ◽  
1999 ◽  
Vol 33 (2) ◽  
pp. 49
Author(s):  
Corine Larchevêque ◽  
A. Casanova
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Aspartic Acid ◽  
Acid Content ◽  
Xylem Sap ◽  
Amino Acid Content ◽  
Total Amino Acid ◽  
Bud Burst ◽  
Phenological Stage ◽  
Total Amino Acid Content

<p style="text-align: justify;">Numerous studies have been carried out on the vine, must and wine, but few on sap. Main phenological stages i.e. bud burst, the flowering period, veraison and the ripening period have been well described. However, at the present time, no study is available identifying the main amino acids of the xylem sap, at the formentioned periods. Located within the « Graves de Pessac-Léognan » vineyard, the plot area studied has a clayey-chalky soil with a small amount of sand present. The cultivar Cabernet franc was grafted on the Fercal rootstock. Planted in June 1982, the vine was trained with Guyot pruning and no tillage. The density of the plantation was 5.550 vines per ha. Four rows of 21 vines were sampled.</p><p style="text-align: justify;">Analysis of xylem sap, by H.P.L.C., at the four stages, taken from several vintages, showed that the global content of amino acids varied year to year (LARCHEVÊQUE, 1998). The experiment reported on herein was carried out in order to determine both the main amino acids in the xylem sap and to identify a marker amongst them, having the same evolution as that of the global amino acid content from one phenological stage to the next. A pool of eight amino acids, all present in large quantities (about 80 p. cent of total amino acid content), was always noted : asparagine/glutamine, tyrosine, arginine, aspartic acid, glutamic acid, histidine and proline. Concentration levels of these components differed in sap, depending on the vintage, at the same phenological stage. At bud burst, as at the flowering period, the sap had a similar qualitative composition.</p><p style="text-align: justify;">It was noteworthy that aspartic acid evidenced the same evolution that of the total amino acid content (i.e. the same variation in percentage) from one phenological stage to the next. Aspartic acid should therefore be regarded as a « marker » of total amino acid content evolution during the vegetative cycle.</p>

scholarly journals Chemical Composition and Amino Acid Content in Different Genotypes of Wheat Flour

2019 ◽  
Author(s):  
Artiona Laze ◽  
Valentina Arapi ◽  
Evelina Ceca ◽  
Kristi Gusho ◽  
Lato Pezo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Total Amino Acid ◽  
Chemical Parameters ◽  
Chromatography Method ◽  
Total Amino Acid Content ◽  
Organically Grown ◽  
Layer Chromatography

The purpose of this study was to evaluate the chemical parameters and amino acid content in 10 genotypes of soft Albanian wheat organically grown and collected during the summer seasons of 2014. The thin layer chromatography method was used for the identification the content of amino acids and spectrophotometry was used to determine the concentration of indentified amino acids. The results exhibited high protein content (13.73 % - 17.32 %) and relative high content of total amino acid content (6.3 % - 10.9 %). The most abundant of all amino acids was glutamic acid (in all genotypes) and cysteine (PZA 1, PZA 2, PZA 3, PZA 4, PZA 5, PZA 6, PZA 7, and PZA 10).

Sign in / Sign up

Export Citation Format

Share Document