scholarly journals High-Resolution Mass Spectrometry for In Vivo Proteome Dynamics using Heavy Water Metabolic Labeling

2020 ◽  
Vol 21 (21) ◽  
pp. 7821
Author(s):  
Rovshan G. Sadygov
Keyword(s):  
Stable Isotopes ◽  
Heavy Water ◽  
Large Scale ◽  
High Resolution Mass Spectrometry ◽  
Mass Resolution ◽  
Mass Analyzer ◽  
Metabolic Labeling ◽  
Spectral Accuracy ◽  
Mass Spectrometers ◽  
Modern Mass

Cellular proteins are continuously degraded and synthesized. The turnover of proteins is essential to many cellular functions. Combined with metabolic labeling using stable isotopes, LC–MS estimates proteome dynamics in high-throughput and on a large scale. Modern mass spectrometers allow a range of instrumental settings to optimize experimental output for specific research goals. One such setting which affects the results for dynamic proteome studies is the mass resolution. The resolution is vital for distinguishing target species from co-eluting contaminants with close mass-to-charge ratios. However, for estimations of proteome dynamics from metabolic labeling with stable isotopes, the spectral accuracy is highly important. Studies examining the effects of increased mass resolutions (in modern mass spectrometers) on the proteome turnover output and accuracy have been lacking. Here, we use a publicly available heavy water labeling and mass spectral data sets of murine serum proteome (acquired on Orbitrap Fusion and Agilent 6530 QToF) to analyze the effect of mass resolution of the Orbitrap mass analyzer on the proteome dynamics estimation. Increased mass resolution affected the spectral accuracy and the number acquired tandem mass spectra.

scholarly journals A method for improvement of mass resolution and isotope accuracy for laser ablation time-of-flight mass spectrometers

2018 ◽  
Vol 33 (1) ◽  
pp. e3081 ◽  
Author(s):  
Reto Wiesendanger ◽  
Marek Tulej ◽  
Valentine Grimaudo ◽  
Alena Cedeño-López ◽  
Rustam Lukmanov ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Time Of Flight ◽  
Mass Resolution ◽  
Mass Spectrometers ◽  
Ablation Time

scholarly journals Simulation and Analysis of GaN Wafer Bowing on Sapphire Substrate

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Wang Bin ◽  
Qu Yu-xuan ◽  
Hu Shi-gang ◽  
Tang Zhi-jun ◽  
Li Jin ◽  
...  
Keyword(s):  
Sapphire Substrate ◽  
Mass Production ◽  
Large Scale ◽  
Lattice Mismatch ◽  
Wafer Surface ◽  
Heteroepitaxial Growth ◽  
Analysis Software ◽  
Element Analysis ◽  
Modern Mass ◽  
The Mathematical Model

During the process of heteroepitaxial growth, if the lattice constant of the growing film differs from that of the substrate, the wafer surface bows, regardless of whether the lattice mismatch occurs or not. As the growth in large-scale wafers speeds up, bowing effects are becoming more and more important. Wafer bowing has a direct impact on the yield in modern mass-production compound semiconductor industries. By using finite element analysis software, the bowing deformation of the GaN wafer on sapphire substrate can be studied. This paper summarizes the causes of bowing deformation, builds the mathematical model, and deduces the relation equation of the wafer bowing. The results show that epitaxial wafer bowing has a linear relationship with the square of the diameter of the substrate but has little relationship with the thickness of the substrate. Moreover, the relation equation of the wafer bowing is also simplified finally.

scholarly journals Microwave Assisted DNA Hydrolysis for Global Methylation Analysis by Gas Chromatography/Tandem Mass Spectrometry

2018 ◽  
Vol 62 (2) ◽  
Author(s):  
Karla Viridiana Castro-Cerritos ◽  
Julio Cesar Torres-Elguera ◽  
Jaqueline Capataz-Tafur ◽  
Erick Adrian Juarez-Arellano ◽  
Adolfo Lopez-Torres
Keyword(s):  
Dna Methylation ◽  
Large Scale ◽  
Limit Of Detection ◽  
Multiple Reaction Monitoring ◽  
Good Alternative ◽  
Detection Methods ◽  
Mass Analyzer ◽  
Global Methylation ◽  
Epigenetic Events ◽  
Methylation Patterns

<div><p class="Abstract">The analysis of the global DNA methylation, calculated as the percentage of 5-methylcytosine (5mC) over the total sum of cytosines, is a well stablished biomarker for monitoring large scale epigenetic events in organisms. DNA purification, hydrolysis, separation and detection methods are critical steps to determine this biomarker. In the present work is proposed a robust procedure for DNA acid-hydrolysis assisted by microwave that provides identical DNA methylation patterns that enzymatic hydrolysis and better release of 5mC than acid classic method. The quantification was performed using a gas chromatographer coupled to a mass spectrometer with triple quadrupole as mass analyzer (GC-TQ-MS/MS) using multiple reaction monitoring (MRM) mode for the trimethylsilyl-derivates of nucleobases; following the transitions of 254→238, 240→170 and 254→238, 254→184 (m/z) for C and 5mC respectively, was achieved a limit of detection of 0.46 fmol for C and 0.41 fmol for 5mC. The proposed procedure is capable of determine 0.004% of 5mC in 50 ng of DNA in a chromatographic time of 10 minutes, being a good alternative to LC-MS/MS analysis.</p></div>

scholarly journals High Resolution Mass Spectrometry Analysis of the Sertraline Residues Contained in the Tissues of Rainbow Trout Reared in Model Experimental Conditions

2020 ◽  
pp. S619-S625
Author(s):  
J VACLAVIK ◽  
P SEHONOVA ◽  
D MEDKOVA ◽  
K STASTNY ◽  
M CHARVATOVA ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Muscle Tissue ◽  
High Resolution Mass Spectrometry ◽  
Negative Impact ◽  
Test Organism ◽  
Internal Standard ◽  
Mass Spectrometry Analysis ◽  
Mass Analyzer ◽  
Ionization Source ◽  
Body Tissues

The growing consumption of pharmaceuticals in the human population and the insufficient efficiency of their elimination in waste water has a long-term negative impact on the environment of aquatic ecosystems, including the organisms that inhabit them. A significant contributor is the consumption of anti-depressants from the SSRI group, which corresponds to their increasing concentration in the environment. The aim of this work was to determine if antidepressant sertraline is able to be stored in fish organisms and to evaluate the content of residues in various body tissues. Rainbow trout (Oncorhynchuss mykkis) was selected as the test organism and was artificially exposed to the antidepressant for 1 month (concentrations 0; 4.2; 44 and 400 ng.g-1 sertraline in the feed). Liver, kidney, brain and muscle tissue biopsies samples were taken for analysis. Detection was performed using an Accela 1250 LC pump and an Accela autosampler coupled with a high-performance mass analyzer with a heated electrospray ionization source Q-Exactive Orbitrap, operating in positive ionization mode and in PRM mode (m/z 306.08108→275.03888 and 309.009991→275.03888 for sertraline and internal standard, respectively). The limit of quantification of the method was 0.1 ng.g-1 of sertraline and the calibration curve showed a good linearity up to 20 ng.g-1. From the collected data, amount of residues was found in the liver, kidney and brain. In contrast, the incidence of residues in muscle tissue was not detected in all groups, which is favorable from the point of view of fish meat consumption, by humans.

Chemical affinity assisted H2 isotope separation using Ca-rich onion-peel-derived nanoporous carbon composite

2021 ◽  
Author(s):  
Raeesh Muhammad ◽  
Suhwan Kim ◽  
Jaewoo Park ◽  
Minji Jung ◽  
Myeongeun Lee ◽  
...  
Keyword(s):  
Stable Isotopes ◽  
Large Scale ◽  
Isotope Separation ◽  
Carbon Composite ◽  
Nanoporous Carbon ◽  
Chemical Affinity ◽  
Scientific Applications ◽  
Onion Peel

Stable isotopes of hydrogen are a prerequisite for many industrial and scientific applications and require their ready supply at a large scale. Herein, we explore the chemical affinity-assisted separation of...

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