scholarly journals Digital Image Analysis Applied to Tumor Cell Proliferation, Aggressiveness, and Migration-Related Protein Synthesis in Neuroblastoma 3D Models

2020 ◽  
Vol 21 (22) ◽  
pp. 8676 ◽  
Author(s):  
Ezequiel Monferrer ◽  
Sabina Sanegre ◽  
Susana Martín-Vañó ◽  
Andrea García-Lizarribar ◽  
Rebeca Burgos-Panadero ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Image Analysis ◽  
Tumor Cell ◽  
Cell Line ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Neuroblastoma Cell ◽  
Tumor Cell Line ◽  
3D Models ◽  
And Migration

Patient-derived cancer 3D models are a promising tool that will revolutionize personalized cancer therapy but that require previous knowledge of optimal cell growth conditions and the most advantageous parameters to evaluate biomimetic relevance and monitor therapy efficacy. This study aims to establish general guidelines on 3D model characterization phenomena, focusing on neuroblastoma. We generated gelatin-based scaffolds with different stiffness and performed SK-N-BE(2) and SH-SY5Y aggressive neuroblastoma cell cultures, also performing co-cultures with mouse stromal Schwann cell line (SW10). Model characterization by digital image analysis at different time points revealed that cell proliferation, vitronectin production, and migration-related gene expression depend on growing conditions and are specific to the tumor cell line. Morphometric data show that 3D in vitro models can help generate optimal patient-derived cancer models, by creating, identifying, and choosing patterns of clinically relevant artificial microenvironments to predict patient tumor cell behavior and therapeutic responses.

Arginine Vasopressin Inhibition of Cyclin D1 Gene Expression Blocks the Cell Cycle and Cell Proliferation in the Mouse Y1 Adrenocortical Tumor Cell Line†

Biochemistry ◽  
2003 ◽  
Vol 42 (7) ◽  
pp. 2116-2121 ◽  
Author(s):  
Telma T. Schwindt ◽  
Fábio L. Forti ◽  
Maria Ap. Juliano ◽  
Luiz Juliano ◽  
Hugo A. Armelin
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Cyclin D1 ◽  
Cell Line ◽  
Arginine Vasopressin ◽  
Tumor Cell Line ◽  
Adrenocortical Tumor

Image analysis of irradiated tumor cell line shows heterogeneity of uPA expression

1994 ◽  
Vol 8 ◽  
pp. 58
Author(s):  
K. Takahashi ◽  
N. Uchida ◽  
T. Kasai ◽  
H.C. Kwaan
Keyword(s):  
Image Analysis ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cell Line

scholarly journals Neferine inhibits growth and migration of gastrointestinal stromal tumor cell line GIST-T1 by up-regulation of miR-449a

2019 ◽  
Vol 109 ◽  
pp. 1951-1959 ◽  
Author(s):  
Fangxi Xue ◽  
Zhaoxia Liu ◽  
Jian Xu ◽  
Xiaoguang Xu ◽  
Xingtian Chen ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Gastrointestinal Stromal Tumor ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Stromal Tumor ◽  
And Migration

PROSTAGLANDIN E2 (PGE2) STIMULATES CELL PROLIFERATION IN THE HUMAN OVARIAN GRANULOSA-LIKE TUMOR CELL LINE KGN

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 196-196
Author(s):  
Chao Jiang ◽  
Lynn Roy ◽  
Steven Remmenga ◽  
John Davis
Keyword(s):  
Cell Proliferation ◽  
Tumor Cell ◽  
Prostaglandin E2 ◽  
Cell Line ◽  
Tumor Cell Line

Inhibitory Effects of Corticosterone on Cell Proliferation and Steroidogenesis in the Mouse Adrenal Tumor Cell Line Y-l

Endocrinology ◽  
1979 ◽  
Vol 104 (2) ◽  
pp. 487-492 ◽  
Author(s):  
EIZO SAITO ◽  
MAKIO MUKAI ◽  
TAKAMURA MURAKI ◽  
YOICHI ICHIKAWA ◽  
MITSUO HOMMA
Keyword(s):  
Cell Proliferation ◽  
Tumor Cell ◽  
Cell Line ◽  
Adrenal Tumor ◽  
Tumor Cell Line ◽  
Inhibitory Effects ◽  
Adrenal Tumor Cell

scholarly journals Cell-Type-Dependent Thyroid Hormone Effects on Glioma Tumor Cell Lines

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Liappas Alexandros ◽  
Mourouzis Iordanis ◽  
Zisakis Athanasios ◽  
Economou Konstantinos ◽  
Lea Robert-William ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Thyroid Hormone ◽  
Tumor Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Tumor Cell Line ◽  
Fold Increase ◽  
Distinct Pattern ◽  
Tumor Cell Lines ◽  
Glioma Tumor

Purpose. The present study investigated the potential effects of long-term T3 treatment on glioma tumor cell lines. Thyroid hormone action on cell growth, differentiation and survival during development may be of therapeutic relevanceMethods and Results1321N1 cell line, an astrocytoma grade II, and U87MG, a glioblastoma grade IV, were exposed for 2 and 4 days in medium deprived of T3 and in medium containing 1 nM T3. T3 promoted re-differentiation in both cell lines. However, T3 increased cell proliferation in 1321N1 (2 days) which declined thereafter (4 days) while in U87MG resulted in suppression of cell proliferation. At the molecular level, a 2.9 fold increase in the expression of TRα1 receptor was observed in U87MG versus 1321N1,P< 0.05. TRβ1 receptor was undetectable. These changes corresponded to a distinct pattern of T3-induced kinase signaling activation; T3 had no effect on ERK activation in both cell lines but significantly increased phospho-Akt levels in 1321N1.Conclusion. In conclusion, T3 can re-differentiate glioma tumor cells, whereas its effect on cell proliferation appears to be dependent on the type of tumor cell line with aggressive tumors being more sensitive to T3. TRα1 receptor may, at least in part, be implicated in this response.

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