scholarly journals Proteome Changes Reveal the Protective Roles of Exogenous Citric Acid in Alleviating Cu Toxicity in Brassica napus L

2021 ◽  
Vol 22 (11) ◽  
pp. 5879
Author(s):  
Young-Hwan Ju ◽  
Swapan Kumar Roy ◽  
Aritra Roy Choudhury ◽  
Soo-Jeong Kwon ◽  
Ju-Young Choi ◽  
...  
Keyword(s):  
Citric Acid ◽  
Brassica Napus ◽  
Molecular Mechanisms ◽  
Vital Role ◽  
Label Free ◽  
Brassica Napus L ◽  
Kegg Pathways ◽  
Cu Stress ◽  
Pathways Analysis ◽  
Proteome Changes

Citric acid (CA), as an organic chelator, plays a vital role in alleviating copper (Cu) stress-mediated oxidative damage, wherein a number of molecular mechanisms alter in plants. However, it remains largely unknown how CA regulates differentially abundant proteins (DAPs) in response to Cu stress in Brassica napus L. In the present study, we aimed to investigate the proteome changes in the leaves of B. L. seedlings in response to CA-mediated alleviation of Cu stress. Exposure of 21-day-old seedlings to Cu (25 and 50 μM) and CA (1.0 mM) for 7 days exhibited a dramatic inhibition of overall growth and considerable increase in the enzymatic activities (POD, SOD, CAT). Using a label-free proteome approach, a total of 6345 proteins were identified in differentially treated leaves, from which 426 proteins were differentially expressed among the treatment groups. Gene ontology (GO) and KEGG pathways analysis revealed that most of the differential abundance proteins were found to be involved in energy and carbohydrate metabolism, photosynthesis, protein metabolism, stress and defense, metal detoxification, and cell wall reorganization. Our results suggest that the downregulation of chlorophyll biosynthetic proteins involved in photosynthesis were consistent with reduced chlorophyll content. The increased abundance of proteins involved in stress and defense indicates that these DAPs might provide significant insights into the adaptation of Brassica seedlings to Cu stress. The abundances of key proteins were further verified by monitoring the mRNA expression level of the respective transcripts. Taken together, these findings provide a potential molecular mechanism towards Cu stress tolerance and open a new route in accelerating the phytoextraction of Cu through exogenous application of CA in B. napus.

Citric acid improves lead (pb) phytoextraction in brassica napus L. by mitigating pb-induced morphological and biochemical damages

2014 ◽  
Vol 109 ◽  
pp. 38-47 ◽  
Author(s):  
Muhammad Bilal Shakoor ◽  
Shafaqat Ali ◽  
Amjad Hameed ◽  
Mujahid Farid ◽  
Sabir Hussain ◽  
...  
Keyword(s):  
Citric Acid ◽  
Brassica Napus ◽  
Brassica Napus L

Bioinformatics studies on structures, functions and diversifications of rolling leaf related genes in rice (Oryza sativa L.)

2020 ◽  
pp. 1-14
Author(s):  
Md. Jahangir Alam ◽  
Md. Alamin ◽  
Most. Humaira Sultana ◽  
Md. Asif Ahsan ◽  
Md. Ripter Hossain ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Molecular Mechanisms ◽  
Oryza Sativa L ◽  
Vital Role ◽  
High Yield ◽  
Leaf Rolling ◽  
Protein Protein Interactions ◽  
Conserved Domains ◽  
Kegg Pathways ◽  
Conserved Domain

Abstract Leaf morphology of crop plants has significant value in agronomy. Leaf rolling in rice plays a vital role to increase grain yield. However, collective information on the rolling leaf (RL) genes reported to date and different comparative bioinformatics studies of their sequences are still incomplete. This bioinformatics study was designed to investigate structures, functions and diversifications of the RL related genes reported till now through several studies. We performed different comparative and functional analyses of the selected 42 RL genes among 103 RL genes using different bioinformatics techniques including gene structure, conserved domain, phylogenetic, gene ontology (GO), transcription factor (TF), Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein–protein network. Exon-intron organization and conserved domain analysis showed diversity in structures and conserved domains of RL genes. Phylogenetic analysis classified the proteins into five major groups. GO and TF analyses revealed that regulation-related genes were remarkably enriched in biological process and 10 different TF families were involved in rice leaf rolling. KEGG analysis demonstrated that 14 RL genes were involved in the KEGG pathways, among which 50% were involved in the metabolism pathways. Of the selected RL proteins, 55% proteins were non-interacting with other RL proteins and OsRL9 was the most interacting RL protein. These results provide important information regarding structures, conserved domains, phylogenetic revolution, protein–protein interactions and other genetic bases of RL genes which might be helpful to the researchers for functional analysis of new candidate RL genes to explore their characteristics and molecular mechanisms for high yield rice breeding.

Proteome Changes in Leaves of Brassica napus L. as a Result of Sclerotinia sclerotiorum Challenge

2008 ◽  
Vol 56 (6) ◽  
pp. 1963-1976 ◽  
Author(s):  
Yue Liang ◽  
Sanjeeva Srivastava ◽  
Muhammad H. Rahman ◽  
Stephen E. Strelkov ◽  
Nat N. V. Kav
Keyword(s):  
Brassica Napus ◽  
Sclerotinia Sclerotiorum ◽  
Brassica Napus L ◽  
Proteome Changes

scholarly journals Genome-wide association study (GWAS) reveals genetic loci of lead (Pb) tolerance during seedling establishment in rapeseed (Brassica napus L.)

2020 ◽  
Author(s):  
Fugui Zhang ◽  
Xin Xiao ◽  
Kun Xu ◽  
Xi Cheng ◽  
Ting Xie ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Study ◽  
Seedling Establishment ◽  
Molecular Mechanisms ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Genetic Loci ◽  
Brassica Napus L ◽  
Pb Stress ◽  
Genome Wide

Abstract Background Lead (Pb) pollution in soil has become one of the major environmental threats to plant growth and human health. Safe utilization of Pb contaminated soil by phytoremediation require Pb tolerant rapeseed ( Brassica napus L.) accessions. However, breeding of new B. napus cultivars tolerance to Pb stress has been restricted by limited knowledge on molecular mechanisms involved in Pb tolerance. This work was carried out to identify genetic loci related to Pb tolerance during seedling establishment in rapeseed. Results Pb tolerance, which was assessed by quantifying radicle length (RL) under 0 or 100 mg/L Pb stress condition, shown an extensive variation in 472 worldwide-collected rapeseed accessions. Based on the criterion of relative RL>80%, six Pb tolerant genotypes were selected. Four quantitative trait loci (QTL) associated with Pb tolerance were identified by Genome-wide association study. The expression level of nine promising candidate genes, including GSTUs , BCATs , UBP13 , TBR and HIPP01 , located in these four QTL regions, were significantly higher or induced by Pb in Pb tolerant accessions in comparison to Pb sensitive accessions. Conclusion To our knowledge, this is the first study on Pb tolerant germplasms and genomic loci in B. napus . The findings can provide valuable genetic resources for the breeding of Pb tolerant B. napus cultivar and understanding of Pb tolerance mechanism in Brassica species.

Citric acid assisted phytoremediation of copper by Brassica napus L.

2015 ◽  
Vol 120 ◽  
pp. 310-317 ◽  
Author(s):  
Ihsan Elahi Zaheer ◽  
Shafaqat Ali ◽  
Muhammad Rizwan ◽  
Mujahid Farid ◽  
Muhammad Bilal Shakoor ◽  
...  
Keyword(s):  
Citric Acid ◽  
Brassica Napus ◽  
Brassica Napus L ◽  
Assisted Phytoremediation

scholarly journals Diagnostic Value of FTIR Spectroscopy, Metabolomic Screening and Molecular Genetics in Saliva for Early Detection of Oral Squamous Cell Carcinoma (OSCC)

2020 ◽  
Vol 6 (3) ◽  
Keyword(s):  
Early Detection ◽  
Oral Cavity ◽  
Ftir Spectroscopy ◽  
Molecular Mechanisms ◽  
Cost Effective ◽  
Diagnostic Value ◽  
Vital Role ◽  
Spectroscopic Technique ◽  
Attenuated Total Reflection ◽  
Label Free

Oral cavity cancer (OCC) has become a prevalent malignancy worldwide. Despite, the current developments of diagnoses and therapies, the 5-year survival rate has persisted at a dismal of 50% in recent decades. Histopathological evaluation remains the golden standard method for cancer detection. However, in some cases the histopathological assessment may not be able to give a definitive diagnosis due to pitfalls in the interpretation of biopsy samples. There are well identified benign conditions in the oral cavity that could mimic malignancy. Hence, it is a timely approach to understand the utility of other emerging techniques which could be used in conjunction with the histopathological assessment. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy has been employed extensively to diagnose various diseases by determining the chemical and molecular alterations. As a cost-effective, minimally invasive or non-invasive and label-free, bio spectroscopic technique this could be developed into an excellent diagnostic tool in the years to come. Besides this, OCC is known to alter the composition of metabolites in saliva. Analysis of the metabolomics in saliva of OCC patients could provide additional information that would be useful to establish a panel of biomarkers with regard to early detection. Furthermore, the rising epidemiological significance underlines the requirement of a better understanding of molecular mechanisms and the recognition of extrapolative tumor markers. Thus, gene expression analysis plays a vital role in identifying those genes related to the progression of this disease. In here, we review the potential applications of FTIR analysis in disease detection and metabolomics in verifying FTIR spectral data. Moreover, the genetic and epigenetic anomalies in OSCC will be briefly discussed along with the salivary biomarkers enabling the detection of this disease.

scholarly journals Genome-wide association study (GWAS) reveals genetic loci of lead (Pb) tolerance during seedling establishment in rapeseed (Brassica napus L.)

2019 ◽  
Author(s):  
Fugui Zhang ◽  
Xin Xiao ◽  
Kun Xu ◽  
Xi Cheng ◽  
Ting Xie ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Study ◽  
Seedling Establishment ◽  
Molecular Mechanisms ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Genetic Loci ◽  
Brassica Napus L ◽  
Pb Stress ◽  
Genome Wide

Abstract Background Lead (Pb) pollution in soil has become one of the major environmental threats to plant growth and human health. Safe utilization of Pb contaminated soil by phytoremediation require Pb tolerant rapeseed ( Brassica napus L.) accessions. However, breeding of new B. napus cultivars tolerance to Pb stress has been restricted by limited knowledge on molecular mechanisms involved in Pb tolerance. This work was carried out to identify genetic loci related to Pb tolerance during seedling establishment in rapeseed.Results Pb tolerance, which was assessed by quantifying radicle length (RL) under 0 or 100 mg/L Pb stress condition, shown an extensive variation in 472 worldwide-collected rapeseed accessions. Based on the criterion of relative RL>80%, six Pb tolerant genotypes were selected. Four quantitative trait loci (QTL) associated with Pb tolerance were identified by Genome-wide association study. The expression level of nine promising candidate genes, including GSTUs , BCATs , UBP13 , TBR and HIPP01 , located in these four QTL regions, were significantly higher or induced by Pb in Pb tolerant accessions in comparison to Pb sensitive accessions.Conclusion To our knowledge, this is the first study on Pb tolerant germplasms and genomic loci in B. napus . The findings can provide valuable genetic resources for the breeding of Pb tolerant B. napus cultivar and understanding of Pb tolerance mechanism in Brassica species.

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