scholarly journals Engineered Microvessel for Cell Culture in Simulated Microgravity

2021 ◽  
Vol 22 (12) ◽  
pp. 6331
Author(s):  
Mei ElGindi ◽  
Ibrahim Hamed Ibrahim ◽  
Jiranuwat Sapudom ◽  
Anna Garcia-Sabate ◽  
Jeremy C. M. Teo
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Simulated Microgravity ◽  
Culture Media ◽  
Large Cell ◽  
Cell Types ◽  
Suitable Material ◽  
Space Flights ◽  
Hypoxic Conditions

As the number of manned space flights increase, studies on the effects of microgravity on the human body are becoming more important. Due to the high expense and complexity of sending samples into space, simulated microgravity platforms have become a popular way to study these effects on earth. In addition, simulated microgravity has recently drawn the attention of regenerative medicine by increasing cell differentiation capability. These platforms come with many advantages as well as limitations. A main limitation for usage of these platforms is the lack of high-throughput capability due to the use of large cell culture vessels. Therefore, there is a requirement for microvessels for microgravity platforms that limit waste and increase throughput. In this work, a microvessel for commercial cell culture plates was designed. Four 3D printable (polycarbonate (PC), polylactic acid (PLA) and resin) and castable (polydimethylsiloxane (PDMS)) materials were assessed for biocompatibility with adherent and suspension cell types. PDMS was found to be the most suitable material for microvessel fabrication, long-term cell viability and proliferation. It also allows for efficient gas exchange, has no effect on cell culture media pH and does not induce hypoxic conditions. Overall, the designed microvessel can be used on simulated microgravity platforms as a method for long-term high-throughput biomedical studies.

scholarly journals Scalable Microgravity Simulator Used for Long-Term Musculoskeletal Cells and Tissue Engineering

2020 ◽  
Vol 21 (23) ◽  
pp. 8908
Author(s):  
Alessandra Cazzaniga ◽  
Fabian Ille ◽  
Simon Wuest ◽  
Carsten Haack ◽  
Adrian Koller ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Simulated Microgravity ◽  
Culture Media ◽  
Three Dimensions ◽  
C2c12 Cells ◽  
Equal Treatment ◽  
New Device ◽  
Microgravity Conditions ◽  
Validation Tests

We introduce a new benchtop microgravity simulator (MGS) that is scalable and easy to use. Its working principle is similar to that of random positioning machines (RPM), commonly used in research laboratories and regarded as one of the gold standards for simulating microgravity. The improvement of the MGS concerns mainly the algorithms controlling the movements of the samples and the design that, for the first time, guarantees equal treatment of all the culture flasks undergoing simulated microgravity. Qualification and validation tests of the new device were conducted with human bone marrow stem cells (bMSC) and mouse skeletal muscle myoblasts (C2C12). bMSC were cultured for 4 days on the MGS and the RPM in parallel. In the presence of osteogenic medium, an overexpression of osteogenic markers was detected in the samples from both devices. Similarly, C2C12 cells were maintained for 4 days on the MGS and the rotating wall vessel (RWV) device, another widely used microgravity simulator. Significant downregulation of myogenesis markers was observed in gravitationally unloaded cells. Therefore, similar results can be obtained regardless of the used simulated microgravity devices, namely MGS, RPM, or RWV. The newly developed MGS device thus offers easy and reliable long-term cell culture possibilities under simulated microgravity conditions. Currently, upgrades are in progress to allow real-time monitoring of the culture media and liquids exchange while running. This is of particular interest for long-term cultivation, needed for tissue engineering applications. Tissue grown under real or simulated microgravity has specific features, such as growth in three-dimensions (3D). Growth in weightlessness conditions fosters mechanical, structural, and chemical interactions between cells and the extracellular matrix in any direction.

A high-throughput cell culture system based on capillary and centrifugal actions for rapid antimicrobial susceptibility testing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 4552-4560
Author(s):  
Taegeun Lim ◽  
Eun-Geun Kim ◽  
Jungil Choi ◽  
Sunghoon Kwon
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Antimicrobial Susceptibility ◽  
Culture System ◽  
Susceptibility Testing ◽  
Culture Media ◽  
Antimicrobial Susceptibility Testing ◽  
Testing System ◽  
Cell Culture System

A capillary and centrifuge-based rapid antimicrobial susceptibility testing system is developed to reduce the time of loading the sample and culture media while achieving a high-throughput testing capacity.

A fast cell loading and high-throughput microfluidic system for long-term cell culture in zero-flow environments

2008 ◽  
Vol 101 (1) ◽  
pp. 190-195 ◽  
Author(s):  
Chunxiong Luo ◽  
Xuejun Zhu ◽  
Tao Yu ◽  
Xianjia Luo ◽  
Qi Ouyang ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Microfluidic System ◽  
Cell Loading ◽  
Zero Flow

scholarly journals Aspiration-mediated hydrogel micropatterning using rail-based open microfluidic devices for high-throughput 3D cell culture

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dohyun Park ◽  
Jungseub Lee ◽  
Younggyun Lee ◽  
Kyungmin Son ◽  
Jin Woo Choi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Capillary Pressure ◽  
Microfluidic Device ◽  
High Throughput ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
Cell Types ◽  
Human Organ ◽  
Patterning Technique ◽  
Experimental Yield

AbstractMicrofluidics offers promising methods for aligning cells in physiologically relevant configurations to recapitulate human organ functionality. Specifically, microstructures within microfluidic devices facilitate 3D cell culture by guiding hydrogel precursors containing cells. Conventional approaches utilize capillary forces of hydrogel precursors to guide fluid flow into desired areas of high wettability. These methods, however, require complicated fabrication processes and subtle loading protocols, thus limiting device throughput and experimental yield. Here, we present a swift and robust hydrogel patterning technique for 3D cell culture, where preloaded hydrogel solution in a microfluidic device is aspirated while only leaving a portion of the solution in desired channels. The device is designed such that differing critical capillary pressure conditions are established over the interfaces of the loaded hydrogel solution, which leads to controlled removal of the solution during aspiration. A proposed theoretical model of capillary pressure conditions provides physical insights to inform generalized design rules for device structures. We demonstrate formation of multiple, discontinuous hollow channels with a single aspiration. Then we test vasculogenic capacity of various cell types using a microfluidic device obtained by our technique to illustrate its capabilities as a viable micro-manufacturing scheme for high-throughput cellular co-culture.

In Vitro Maintenance of Amoebocytes from the American Oyster (Crassostrea virginica)

1979 ◽  
Vol 36 (4) ◽  
pp. 461-467 ◽  
Author(s):  
Frank Brewster ◽  
Bruce L. Nicholson
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Basal Medium ◽  
Cell Types ◽  
Heart Tissue ◽  
Oyster Tissue ◽  
Protein Digests ◽  
Dividing Cells ◽  
Fetal Bovine

Explant and monolayer cell cultures were initiated from oyster heart and embryonic tissue and maintained for periods of a few days to 6 mo depending on the type of tissue and the culture medium. A pH of 7.0–7.3 and a temperature of 20 °C were optimum. Vertebrate cell culture media prepared in a marine saline and supplemented with fetal bovine serum and protein digests provided a suitable basal medium. Supplementation of the basal medium with oyster hemolymph or extracts of oyster tissue markedly prolonged cell maintenance. Explant cultures of heart tissue with the subsequent outward migration of individual cells were most easily initiated and maintained for periods up to 6 mo. Although several cell types were observed, actively motile, granular amoebocytes predominated. No mitotic cells were observed even following exposure to a variety of mitogens. Cultures initiated from disaggregated larvae did yield actively dividing cells. Key words: oyster, cell culture, amoebocytes

scholarly journals Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.

2020 ◽  
Author(s):  
Razieh Alipour ◽  
Alimohammad Fatemi ◽  
Fereshteh Alsahebfosul ◽  
Alireza Andalib ◽  
Abbasali Pourazar
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Culture Media ◽  
Calf Serum ◽  
Cell Types ◽  
Autologous Serum ◽  
Autologous Plasma ◽  
Cd11b Expression ◽  
Suitable Alternative ◽  
Neutrophil Cell

Abstract Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.

scholarly journals Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.

2019 ◽  
Author(s):  
Razieh Alipour ◽  
Alimohammad Fatemi ◽  
Fereshteh Alsahebfosul ◽  
Alireza Andalib ◽  
Abbasali Pourazar
Keyword(s):  
Cell Culture ◽  
Fetal Calf Serum ◽  
Culture Media ◽  
Calf Serum ◽  
Cell Types ◽  
Autologous Serum ◽  
Autologous Plasma ◽  
Cd11b Expression ◽  
Suitable Alternative ◽  
Neutrophil Cell

Abstract Objective Currently the replacement of fetal calf serum (FCS) by a more suitable alternative is a sought aim in the field of tissue and cell culture research. Autologous plasma (AP) and especially autologous serum (AS) have been shown to be effective substitutes of FCS in culture media for some of cell types. Nevertheless, there is no comparative data on the most appropriate supplement for cell media in neutrophil studies, it is now unclear whether AP have relatively an equal, superior or inferior performance to FCS in neutrophil cell culture. In the present study, human blood neutrophils were isolated and cultured in FCS- or AP-supplemented medium. After 12, 36 and 60 hours of incubation, cell viability, oxidative burst and CD11b expression were determined by flow cytometry.Results Compared to the culture of neutrophils in FCS 10% medium, the culture of neutrophils in a medium with AP 10% could prolong their life span without affecting their function. The findings introduce AP as a better supplement for human neutrophil cell culture than FCS and propose a simple and economical procedure for neutrophil isolation and culture.

Fluid Lipid Multilayer Stabilization by Tetraethyl Orthosilicate for Underwater AFM Characterization and Cell Culture Applications

MRS Advances ◽  
2017 ◽  
Vol 2 (57) ◽  
pp. 3553-3558
Author(s):  
Aubrey E. Kusi-Appiah ◽  
Troy W. Lowry ◽  
Nicholas Vafai ◽  
David H. Van Winkle ◽  
Steven Lenhert
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cell Types ◽  
Tetraethyl Orthosilicate ◽  
Force Microscopy ◽  
Essential Step ◽  
Atomic Force ◽  
Lipid Films ◽  
Cell Assays

ABSTRACTStabilization of surface supported fluid lipid multilayers for underwater characterization is an essential step in making them useful for scalable cell culture applications such as high throughput screening. To this end, we used tetraethyl orthosilicate (TEOS), recently shown to stabilize fluid lipid films while maintaining their fluidity and functionality under water, to stabilize lipid multilayer micropatterns of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The treated multilayers were immersed under water and successfully imaged by atomic force microscopy (AFM), a difficult feat to perform on fluid lipid multilayers without TEOS treatment. The treated lipid multilayer showed an average swelling of approximately 18% in water but remained stable during the imaging process. The TEOS-treated lipid multilayers also proved compatible with cell culture as HeLa, MDCK, and HEK cell types all adhered and grew in high numbers over the multilayers. The results obtained here open the door to the use of fluid lipid multilayers in biotechnology applications such as microarray based high throughput cell assays.

Sign in / Sign up

Export Citation Format

Share Document