scholarly journals Effects of 1,2-Dimethylhydrazine on Barrier Properties of Rat Large Intestine and IPEC-J2 Cells

2021 ◽  
Vol 22 (19) ◽  
pp. 10278
Author(s):  
Viktoria Bekusova ◽  
Linda Droessler ◽  
Salah Amasheh ◽  
Alexander G. Markov
Keyword(s):  
Colorectal Cancer ◽  
Electrical Resistance ◽  
Barrier Properties ◽  
Acute Effect ◽  
Paracellular Permeability ◽  
Transepithelial Electrical Resistance ◽  
Rat Colon ◽  
Sodium Fluorescein ◽  
Cell Monolayers

Colon cancer is accompanied by a decrease of epithelial barrier properties, which are determined by tight junction (TJ) proteins between adjacent epithelial cells. The aim of the current study was to analyze the expression of TJ proteins in a rat model of 1,2-dimethylhydrazine (DMH)-induced colorectal cancer, as well as the barrier properties and TJ protein expression of IPEC-J2 cell monolayers after incubation with DMH. Transepithelial electrical resistance and paracellular permeability for sodium fluorescein of IPEC-J2 were examined by an epithelial volt/ohm meter and spectrophotometry. The expression and localization of TJ proteins were analyzed by immunoblotting and immunohistochemistry. In the colonic tumors of rats with DMH-induced carcinogenesis, the expression of claudin-3 and -4 was significantly increased compared to controls. The transepithelial electrical resistance of IPEC-J2 cells increased, while paracellular permeability for sodium fluorescein decreased, accompanied by an increased expression of claudin-4. The increase of claudin-4 in rat colon after chronic DMH exposure was consistent with the acute effect of DMH on IPEC-J2 cells, which may indicate an essential role of this protein in colorectal cancer development.

Copper treatment alters the barrier functions of human intestinal Caco-2 cells: involving tight junctions and P-glycoprotein

2004 ◽  
Vol 23 (8) ◽  
pp. 369-377 ◽  
Author(s):  
Zhiwei Liu ◽  
Bingheng Chen
Keyword(s):  
Electrical Resistance ◽  
Paracellular Permeability ◽  
Transepithelial Electrical Resistance ◽  
Complete Medium ◽  
Glycoprotein P ◽  
Barrier Functions ◽  
Cell Monolayers ◽  
Copper Treatment ◽  
P Glycoprotein ◽  
Junctional Protein

This study investigated the effects of copper on paracellular permeability and P-glycoprotein (P-gp) in Caco-2 cells. Apical treatment with 100–300,μM CuS04 in Hanks' balanced salt solution (HBSS, up to 3 hours) induced a time-and concentration-dependent increase in permeability of Caco-2 cell monolayers monitored by transepithelial electrical resistance (TEER). Copper treatment also induced a concentration-dependent reduction of F-actin stain, but not of tight junctional protein ZO-1. In addition, without any adverse effects on TEER, apical treatment with 300 μM CuS04 in complete medium (for 24 hours) could reduce basolateral-to-apical transport, and increase apical-to-basolateral transport of rhodamine-123 (Rho-123) and accumulation of Rho-123 in Caco-2 cells. Treatment with 10-100 μM CuS04 in HBSS (up to 3 hours) also induced a time-and concentration-dependent increase in accumulation of Rho-123 in Caco-2 cells. The results indicated that copper treatment increased the paracellular perme-ability probably by perturbing F-actin skeleton, and inhibited P-gp, thus altering the barrier functions of Caco-2 cells.

scholarly journals Application of Measurements of Transepithelial Electrical Resistance of Intestinal Epithelial Cell Monolayers To Evaluate Probiotic Activity

2005 ◽  
Vol 71 (11) ◽  
pp. 7528-7530 ◽  
Author(s):  
Trine Danø Klingberg ◽  
Maja Herold Pedersen ◽  
Avrelija Cencic ◽  
Birgitte Bjørn Budde
Keyword(s):  
Electrical Resistance ◽  
Intestinal Epithelial Cell ◽  
Transepithelial Electrical Resistance ◽  
Intestinal Epithelial ◽  
Lactobacillus Salivarius ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Probiotic Lactobacilli ◽  
Dose Dependent ◽  
Probiotic Activity

ABSTRACT Among five potentially probiotic lactobacilli investigated, Lactobacillus plantarum MF1298 and Lactobacillus salivarius DC5 showed the highest increase in the transepithelial electrical resistance (TER) of polarized monolayers of Caco-2 cells, and this increase was shown to be dose dependent. Furthermore, preincubation with MF1298 attenuated a decrease in TER induced by Listeria monocytogenes.

scholarly journals Effects of Yeast and Bacterial Commensals and Pathogens of the Female Genital Tract on the Transepithelial Electrical Resistance of HeLa Cells

2016 ◽  
Vol 10 (1) ◽  
pp. 90-96 ◽  
Author(s):  
Vassiliki Tsata ◽  
Aristea Velegraki ◽  
Anastasios Ioannidis ◽  
Cornelia Poulopoulou ◽  
Pantelis Bagos ◽  
...  
Keyword(s):  
Electrical Resistance ◽  
Genital Tract ◽  
Female Genital Tract ◽  
Transepithelial Electrical Resistance ◽  
Host Immune System ◽  
Opportunistic Pathogens ◽  
Selective Effect ◽  
Cell Monolayers ◽  
Female Genital

Commensals of the human body can shift to a pathogenic phase when the host immune system is impaired. This study aims to investigate the effect of seven yeast and two bacterial commensals and opportunistic pathogens isolated from blood and the female genital tract on the transepithelial electrical resistance (TER) of human cervical epithelial cell cultures (HeLa). The pathogensCandida tropicalis,C. parapsilosis,C. glabrata, C. krusei, C. albicansandSaccharomyces cerevisiae, caused a significant decrease in TER as compared to the controls;Lactobacillusspp caused a significant increase in TERversusthe controls andEscherichia colihad no effect on the TER of the cell monolayers. The above data show thatCandidaspp.,S. cerevisiaeandLactobacillusspp. have a non-selective effect on the TER of HeLa cell monolayers. These results are consistent with thein vivonon-selective action of these microorganisms on the various human mucosal epithelia.

scholarly journals Heterogeneity of the barrier properties of the colon in rat

2021 ◽  
Vol 66 (2) ◽  
Author(s):  
Viktoria Bekusova ◽  
Ilyas Fatyykhov ◽  
Salah Amasheh ◽  
Alexander Markov
Keyword(s):  
Distal Part ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Barrier Properties ◽  
Proximal Part ◽  
Transepithelial Electrical Resistance ◽  
Sodium Fluorescein ◽  
Ussing Chambers ◽  
Depth Analysis ◽  
Different Parts

The incidence of colorectal cancer in different parts of the large intestine is not the same, as tumors more often appear in the distal part of the colon compared to the proximal one. The purpose of this study was to investigate heterogeneity of the barrier properties of the colon and clarify the effects of Prostaglandin E2 and interleukin-1beta on its different parts. An in-depth analysis of short circuit current, transepithelial electrical resistance and paracellular permeability for sodium fluorescein in Ussing chambers showed that the proximal part of the colon was less permeable compared to the distal one and the substances had different effects on the parameters of permeability in different parts of the colon. We suppose that heterogeneity of the barrier properties of the colon and various effects of their regulation by local molecular agents may determine different incidence of pathologies in the colon.

scholarly journals Adherence to and Penetration of Human Intestinal Caco-2 Epithelial Cell Monolayers by Pseudomonas aeruginosa

1998 ◽  
Vol 66 (4) ◽  
pp. 1748-1751 ◽  
Author(s):  
Yoichi Hirakata ◽  
Kohichi Izumikawa ◽  
Toshiyuki Yamaguchi ◽  
Shizunobu Igimi ◽  
Nobuhiko Furuya ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cell ◽  
Virulence Factor ◽  
Electrical Resistance ◽  
Transepithelial Electrical Resistance ◽  
Clinical Isolates ◽  
Exotoxin A ◽  
Cell Monolayers ◽  
Drastic Decrease ◽  
Epithelial Cell Monolayers

ABSTRACT Clinical isolates of Pseudomonas aeruginosa from blood adhered to and penetrated intestinal Caco-2 cell monolayers to a greater degree than did isolates from sputum, with a concomitant drastic decrease in transepithelial electrical resistance. PAO-PR1, an avirulent exotoxin A mutant of PAO1, did not cause a decrease in the resistance. The Caco-2 monolayer system may be useful for the evaluation of certain P. aeruginosa virulence factor activities.

scholarly journals c-Jun NH2-terminal kinase-2 mediates osmotic stress-induced tight junction disruption in the intestinal epithelium

2010 ◽  
Vol 299 (3) ◽  
pp. G572-G584 ◽  
Author(s):  
G. Samak ◽  
T. Suzuki ◽  
A. Bhargava ◽  
R. K. Rao
Keyword(s):  
Osmotic Stress ◽  
Actin Cytoskeleton ◽  
Restraint Stress ◽  
Paracellular Permeability ◽  
Cell Monolayers ◽  
Cold Restraint Stress ◽  
Jnk Activation ◽  
Mouse Ileum ◽  
Cold Restraint

Gastrointestinal epithelium faces osmotic stress, both at physiological and pathophysiological conditions. JNK activation is an immediate cellular response to osmotic stress. We investigated the effect of osmotic stress on intestinal epithelial barrier function and delineated the role of JNK2 in osmotic stress-induced tight junction (TJ) regulation in Caco-2 cell monolayers and ileum of Jnk −/− and Jnk2 −/− mice. The role of JNK activation in osmotic stress-induced TJ disruption was evaluated using JNK-specific inhibitor and antisense oligonucleotides. Furthermore, the effect of cold restraint stress in vivo on TJ integrity was determined in rats. Osmotic stress disrupted TJs and barrier function in Caco-2 cell monolayers without affecting cell viability. Osmotic stress activated JNK1 and JNK2 and the inhibition of JNK by SP600125 attenuated osmotic stress-induced TJ disruption. TJ disruption and barrier dysfunction by osmotic stress was associated with JNK-dependent remodeling of actin cytoskeleton. Knockdown of JNK2 accelerated TJ assembly and attenuated osmotic stress-induced TJ disruption in Caco-2 cell monolayers. In mouse ileum in vitro, osmotic stress increased paracellular permeability, which was attenuated by SP600125. Osmotic stress disrupted actin cytoskeleton and TJs and increased paracellular permeability in the ileum of wild-type and JNK1 −/− mice, but not in JNK2 −/− mouse ileum. Cold restraint stress activated JNK in rat ileum and caused JNK-dependent remodeling of actin cytoskeleton and redistribution of occludin and zona occluden-1 from the intercellular junctions. These results reveal the role of JNK2 in the mechanism of osmotic stress-induced TJ disruption in the intestinal epithelium.

scholarly journals Measurements of transepithelial electrical resistance (TEER) are affected by junctional length in immature epithelial monolayers

2021 ◽  
Author(s):  
Kannapin Felix ◽  
Schmitz Tobias ◽  
Hansmann Jan ◽  
Schlegel Nicolas ◽  
Meir Michael
Keyword(s):  
Epithelial Cell ◽  
Electrical Resistance ◽  
Fluorescein Isothiocyanate ◽  
Cell Types ◽  
Transepithelial Electrical Resistance ◽  
Cell Junctions ◽  
Junctional Complex ◽  
Additional Increase ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers

AbstractThe measurement of transepithelial electrical resistance (TEER) is a common technique to determine the barrier integrity of epithelial cell monolayers. However, it is remarkable that absolute TEER values of similar cell types cultured under comparable conditions show an immense heterogeneity. Based on previous observations, we hypothesized that the heterogeneity of absolute TEER measurements can not only be explained by maturation of junctional proteins but rather by dynamics in the absolute length of cell junctions within monolayers. Therefore, we analyzed TEER in epithelial cell monolayers of Caco2 cells during their differentiation, with special emphasis on both changes in the junctional complex and overall cell morphology within monolayers. We found that in epithelial Caco2 monolayers TEER increased until confluency, then decreased for some time, which was then followed by an additional increase during junctional differentiation. In contrast, permeability of macromolecules measured at different time points as 4 kDA fluorescein isothiocyanate (FITC)-dextran flux across monolayers steadily decreased during this time. Detailed analysis suggested that this observation could be explained by alterations of junctional length along the cell borders within monolayers during differentiation. In conclusion, these observations confirmed that changes in cell numbers and consecutive increase of junctional length have a critical impact on TEER values, especially at stages of early confluency when junctions are immature.

Sign in / Sign up

Export Citation Format

Share Document