scholarly journals A Fast and Reliable Process to Fabricate Regenerated Silk Fibroin Solution from Degummed Silk in 4 Hours

2021 ◽  
Vol 22 (19) ◽  
pp. 10565
Author(s):  
Michael Wöltje ◽  
Arthur Kölbel ◽  
Dilbar Aibibu ◽  
Chokri Cherif
Keyword(s):  
Silk Fibroin ◽  
Biomedical Applications ◽  
Processing Time ◽  
Gel Filtration ◽  
Process Time ◽  
Short Term ◽  
Regenerated Silk Fibroin ◽  
Total Process ◽  
Fibroin Solution

Silk fibroin has a high potential for use in several approaches for technological and biomedical applications. However, industrial production has been difficult to date due to the lengthy manufacturing process. Thus, this work investigates a novel procedure for the isolation of non-degraded regenerated silk fibroin that significantly reduces the processing time from 52 h for the standard methods to only 4 h. The replacement of the standard degumming protocol by repeated short-term microwave treatments enabled the generation of non-degraded degummed silk fibroin. Subsequently, a ZnCl2 solution was used to completely solubilize the degummed fibroin at only 45 °C with an incubation time of only 1 h. Desalting was performed by gel filtration. Based on these modifications, it was possible to generate a cytocompatible aqueous silk fibroin solution from degummed silk within only 4 h, thus shortening the total process time by 48 h without degrading the quality of the isolated silk fibroin solution.

Study on the Mechanical Property of CNTs/Silk Fibroin Composite Fiber

2014 ◽  
Vol 1004-1005 ◽  
pp. 577-580
Author(s):  
Yu Chun Jiang ◽  
Yong Xiang Huang ◽  
Yuan Song Zhang
Keyword(s):  
Mechanical Property ◽  
Carbon Nanotubes ◽  
Carbon Nanotube ◽  
Silk Fibroin ◽  
Composite Fiber ◽  
Preparation Conditions ◽  
Regenerated Silk Fibroin ◽  
Excellent Mechanical Property ◽  
New Type ◽  
Fibroin Solution

Carbon nanotube (CNT) is a new type of nanoscale materials, which has excellent adsorption and mechanical properties. In this paper, carbon nanotubes were added in the regenerated silk fibroin solution to get CNTs/silk fibroin composite fiber, which had more excellent mechanical property than the regenerated fibroin fiber. The results indicate that the optimum preparation conditions are summed up as follows: silk fibroin concentration 35%, spinning temperature 40oC, carbon nanotube mass fraction 1.0% and the longest storage time less than 7 days. The mechanical property of regenerated fibroin fiber which added with carbon nanotubes is obviously improved.

Bio-doping of regenerated silk fibroin solution and films: a green route for biomanufacturing

RSC Advances ◽  
2014 ◽  
Vol 4 (64) ◽  
pp. 33687-33694 ◽  
Author(s):  
A. Sagnella ◽  
C. Chieco ◽  
N. Di Virgilio ◽  
S. Toffanin ◽  
T. Posati ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Rhodamine B ◽  
Optically Active ◽  
Green Route ◽  
Regenerated Silk Fibroin ◽  
Fibroin Solution

Optically active silk fibroin films could be successfully obtained by biodoping, i.e. from cocoons of Bombyx mori fed with a rhodamine B-added diet.

scholarly journals Investigations on rheological properties and gelation of tasar regenerated silk fibroin solution

2013 ◽  
Vol 131 (7) ◽  
pp. n/a-n/a
Author(s):  
Yogesha Lakkegowda ◽  
Raghu Ammannappa ◽  
Sharath Ananthamurthy
Keyword(s):  
Rheological Properties ◽  
Silk Fibroin ◽  
Regenerated Silk Fibroin ◽  
Fibroin Solution

Fabrication of Organic Silk Fibroin/Multiwalled Carbon Nanotube Composite Cryogels by Freeze-Drying Method

2008 ◽  
Vol 47-50 ◽  
pp. 1105-1108 ◽  
Author(s):  
Soon Min Kwon ◽  
Hun Sik Kim ◽  
Hyoung Joon Jin
Keyword(s):  
Electron Microscopy ◽  
Silk Fibroin ◽  
Freeze Drying ◽  
Sol Gel ◽  
Three Dimensional ◽  
Nitrogen Adsorption ◽  
Network Structures ◽  
Multiwalled Carbon ◽  
Regenerated Silk Fibroin ◽  
Fibroin Solution

The multiwalled carbon nanotubes (MWCNTs) incorporated organic silk fibroin cryogels were fabricated through sol-gel process of aqueous silk fibroin solution, followed by freeze-drying. The MWCNT incorporated silk fibroin hydrogel was prepared by the regeneration of silk fibroin using an aqueous silk fibroin solution in which MWCNTs were dispersed by in-situ methods. The morphology and microstructure of the silk fibroin network structure were characterized using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). The FESEM and TEM images show that the cryogels had three-dimensional network structures and the MWCNTs were well embedded in the network structures of regenerated silk fibroin. The porous properties of the MWCNT incorporated silk fibroin cryogels were investigated by nitrogen adsorption-desorption technique. The MWCNTs increased the proportions of micropores and mesopores in the silk fibroin cryogels when they were introduced in the appropriate amounts.

Effect of Concentration on Structure and Properties of Concentrated Regenerated Silk Fibroin Solution

2011 ◽  
Vol 311-313 ◽  
pp. 1653-1656 ◽  
Author(s):  
Fang Xie ◽  
Hao Liang
Keyword(s):  
Silk Fibroin ◽  
Conformational Transition ◽  
Random Coil ◽  
Scanning Calorimetry ◽  
Regenerated Silk Fibroin ◽  
Lower Temperature ◽  
Α Helix ◽  
Β Sheet ◽  
Dsc Curves ◽  
Fibroin Solution

The thermal properties and rheological behavior of concentrated regenerated silk fibroin aqueous solution from 15% to 37% was investigated by differential scanning calorimetry (DSC) and rheometer. Also the conformation of solutions was characterized by Raman spectra. It was discovered that the major endothermic peak in the DSC curves shifted toward the lower temperature region with increasing the concentration. This behavior suggests increasing the concentration can accelerate conformational transition of silk fibroin from random coil and α-helix to β-sheet structure. In addition, it was found that the viscosity of solution increased with increasing concentration in favor of spinning.

Study on Improving Biodegradation Process of Regenerated Silk Fibroin Fiber

2011 ◽  
Vol 175-176 ◽  
pp. 266-271
Author(s):  
Zheng Hua Zhu ◽  
Xuan Lu ◽  
Xiao Hong Zhou
Keyword(s):  
Bombyx Mori ◽  
Calcium Chloride ◽  
Silk Fibroin ◽  
Degradation Rate ◽  
Chloride Concentration ◽  
High Strength ◽  
Silk Fibroin Fiber ◽  
Regenerated Silk Fibroin ◽  
Biodegradation Process ◽  
Fibroin Solution

The native silk fibroin fiber from Bombyx mori has been used as suture with non-degradable character. Therefore, preparing biodegradable silk fibroin fiber is very attractive and important. In this paper, the biodegradable silk fibroin fiber with high strength was prepared according to the process of regenerated silk fibroin fiber from the 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) solution and by adding calcium chloride CaCl2 to the silk fibroin solution. The degradation rate of the regenerated silk fibroin fibers was dependent on calcium chloride concentration.

scholarly journals Effect of degumming ph value on electrospining of silk fibroin

2014 ◽  
Vol 18 (5) ◽  
pp. 1703-1704 ◽  
Author(s):  
Shen-Zhou Lu ◽  
Xiao-Ping Zhang ◽  
Juan Wang ◽  
Tie-Ling Xing ◽  
Jian Jin
Keyword(s):  
Molecular Weight ◽  
Silk Fibroin ◽  
Gel Electrophoresis ◽  
Ph Value ◽  
Low Concentration ◽  
Regenerated Silk Fibroin ◽  
Fibroin Solution

Regenerated silk fibroin fibers show properties dependent on the molecular weight of fibroin. The cocoon-degumming approaches had great impact on the degradation of silk fibroin. The effect of degumming pH value to electrospining of fibroin was studied in this paper. The viscosity and molecular weight of regenerated silk fibroin were studied using rheometer and gel electrophoresis. The results showed that the weaker the alkalinity of degumming reagent, there was the milder the effect on silk fibroin molecular. The fibroin fibers can be prepared by electrospining with low concentration of regenerated silk fibroin solution.

A bio-inspired microfluidic concentrator for regenerated silk fibroin solution

2012 ◽  
Vol 162 (1) ◽  
pp. 435-440 ◽  
Author(s):  
Jie Luo ◽  
Yaopeng Zhang ◽  
Yan Huang ◽  
Huili Shao ◽  
Xuechao Hu
Keyword(s):  
Silk Fibroin ◽  
Regenerated Silk Fibroin ◽  
Fibroin Solution
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