Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum

Over the last three decades, there has been special interest in developing drugs that mimic the characteristics of natural tears for use it in the treatment of several ocular surface disorders. Interestingly, the composition of blood plasma is very similar to tears. Therefore, different blood-derived products like autologous serum (AS) and plasma rich in growth factors (PRGF) have been developed for the treatment of diverse ocular pathologies. However, scarce studies have been carried out to analyze the differences between both types of blood-derived products. In the present study, blood from three healthy donors was drawn and processed to obtain AS and PRGF eye drops. Then, human corneal stromal keratocytes (HK) were treated with PRGF or undiluted AS. Proteomic analysis was carried out to analyze and characterize the differential protein profiles between PRGF and AS, and the differentially expressed proteins in HK cells after PRGF and AS treatment. The results obtained in the present study show that undiluted AS induces the activation of different pathways related to an inflammatory, angiogenic, oxidative stress and scarring response in HK cells regarding PRGF. These results suggest that PRGF could be a better alternative than AS for the treatment of ocular surface disorders.

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Plasma rich in growth factors (PRGF) eye drops stimulates scarless regeneration compared to autologous serum in the ocular surface stromal fibroblasts

Experimental Eye Research ◽

10.1016/j.exer.2015.02.016 ◽

2015 ◽

Vol 135 ◽

pp. 118-126 ◽

Cited By ~ 40

Author(s):

E. Anitua ◽

M. de la Fuente ◽

F. Muruzabal ◽

A. Riestra ◽

J. Merayo-Lloves ◽

...

Keyword(s):

Growth Factors ◽

Ocular Surface ◽

Autologous Serum ◽

Eye Drops ◽

Stromal Fibroblasts

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Comparison of treatment efficacy between 100% platelet-rich plasma and 100% serum eye drops in moderate-to-severe dry eye disease: a randomised controlled trial protocol

BMJ Open ◽

10.1136/bmjopen-2020-048479 ◽

2021 ◽

Vol 11 (6) ◽

pp. e048479

Author(s):

Passara Jongkhajornpong ◽

Pawin Numthavaj ◽

Thunyarat Anothaisintawee ◽

Kaevalin Lekhanont ◽

Gareth McKay ◽

...

Keyword(s):

Dry Eye ◽

Ocular Surface ◽

Platelet Rich Plasma ◽

Controlled Trial ◽

Dry Eye Disease ◽

Autologous Serum ◽

Eye Disease ◽

Eye Drops ◽

Department Of Ophthalmology ◽

Autologous Platelet

IntroductionDry eye disease (DED) is a common eye problem. Although the disease is not fatal, it substantially reduces quality of life and creates a high economic burden, especially in patients with moderate-to-severe DED. Several biological tear substitutes (eg, autologous serum (AS), autologous platelet-rich plasma (APRP) and autologous platelet lysate) could effectively improve dry eyes. However, evidence on their comparative efficacy is controversial. This study aims to compare the efficacy of 100% APRP with 100% AS eye drops in patients with moderate-to-severe DED.Methods and analysisThe study is a single-centre, double-blinded randomised, parallel, non-inferiority trial. One hundred and thirty patients with moderate-to-severe DED, aged 18–70 years will be recruited from outpatient clinic, Department of Ophthalmology, Ramathibodi Hospital, Bangkok from February 2021 to January 2023. Patients will be randomised to receive either 100% APRP or 100% AS eye drops (1:1 ratio) for 4 weeks. The primary outcomes are ocular surface disease index (OSDI) and ocular surface staining (OSS) evaluated using the Oxford scale. Secondary outcomes are fluorescein break-up time, Schirmer’s I test, meibomian gland parameters and adverse events. Other measured outcomes include best-corrected visual acuity, intraocular pressure and compliance.Ethics and disseminationThe study protocol and any supplements used in conducting this trial have been approved by the Ethics Committee of Faculty of Medicine, Ramathibodi Hospital, Mahidol University (MURA2020/1930). Informed consent will be obtained from all patients before study entry. Results will be presented in peer-reviewed journals and international conferences.Trial registration numberNCT04683796.

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The effects of autologous serum eye drops in the treatment of ocular surface diseases : a retrospective study

Acta Ophthalmologica ◽

10.1111/j.1755-3768.2012.2635.x ◽

2012 ◽

Vol 90 ◽

pp. 0-0

Author(s):

E BASLI ◽

L LAROCHE ◽

M LAURENT ◽

MC DESPIAU ◽

V BORDERIE

Keyword(s):

Retrospective Study ◽

Ocular Surface ◽

Autologous Serum ◽

Eye Drops ◽

Ocular Surface Diseases ◽

Serum Eye Drops

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Stability of freeze-dried plasma rich in growth factors eye drops stored for 3 months at different temperature conditions

European Journal of Ophthalmology ◽

10.1177/1120672120913035 ◽

2020 ◽

pp. 112067212091303 ◽

Cited By ~ 3

Author(s):

Eduardo Anitua ◽

María de la Fuente ◽

Francisco Muruzábal ◽

Jesús Merayo-Lloves

Keyword(s):

Growth Factors ◽

Room Temperature ◽

Storage Condition ◽

Eye Drops ◽

Temperature Conditions ◽

Healthy Donors ◽

Freeze Dried ◽

And Storage ◽

Blood Centrifugation ◽

Human Keratocytes

Purpose: The purpose of this study was to analyze the biological content and activity of freeze-dried plasma rich in growth factors eye drops after their storage at 4°C and at room temperature for 3 months with respect to fresh samples (time 0). Methods: Plasma rich in growth factors was obtained after blood centrifugation from three healthy donors. After platelet activation, the obtained plasma rich in growth factors eye drops were lyophilized alone or in combination with lyoprotectant (trehalose), then they were stored for 3 months at room temperature or at 4°C. Several growth factors were analyzed at each storage time and condition. Furthermore, the proliferative and migratory potential of freeze-dried plasma rich in growth factors eye drops kept for 3 months at different temperature conditions was evaluated on primary human keratocytes. Results: The different growth factors analyzed maintained their levels at each time and storage condition. Freeze-dried plasma rich in growth factors eye drops stored at room temperature or 4°C for 3 months showed no significant differences on the proliferative activity of keratocytes in comparison with fresh samples. However, the number of migratory human keratocytes increased significantly after treatment with lyophilized plasma rich in growth factors eye drops kept for 3 months compared to those obtained at time 0. No significant differences were observed between the freeze-dried plasma rich in growth factors eye drops whether mixed or not with lyoprotectant. Conclusion: Freeze-dried plasma rich in growth factors eye drops preserve the main growth factors and their biological activity after storage at room temperature or 4°C for up to 3 months. Lyophilized plasma rich in growth factors eye drops conserve their biological features even without the use of lyoprotectants for at least 3 months.

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Evaluation of the Efficacy of 50% Autologous Serum Eye Drops in Different Ocular Surface Pathologies

BioMed Research International ◽

10.1155/2014/826970 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 18

Author(s):

Francesco Semeraro ◽

Eliana Forbice ◽

Osvaldo Braga ◽

Alessandro Bova ◽

Attilio Di Salvatore ◽

...

Keyword(s):

Ocular Surface ◽

Conventional Therapy ◽

Keratoconjunctivitis Sicca ◽

Signs And Symptoms ◽

Autologous Serum ◽

Eye Drops ◽

Blurred Vision ◽

Group 2 ◽

Serum Eye Drops ◽

Group 1

Purpose. This study evaluated the efficacy of 50% autologous serum eye drops in ocular surface diseases not improved by conventional therapy.Methods. We analyzed two groups: (1) acute eye pathologies (e.g., chemical burns) and (2) chronic eye pathologies (e.g., recurrent corneal erosion, neurotropic keratitis, and keratoconjunctivitis sicca). The patients were treated for surface instability after conventional therapy. The patients received therapy 5 times a day until stabilization of the framework; they then reduced therapy to 3 times a day for at least 3 months. We analyzed the best corrected visual acuity, epithelial defects, inflammation, corneal opacity, and corneal neovascularization. We also analyzed symptoms such as tearing, burning, sense of foreign body or sand, photophobia, blurred vision, and difficulty opening the eyelids.Results. We enrolled 15 eyes in group 1 and 11 eyes in group 2. The average therapy period was 16 ± 5.86 weeks in group 1 and 30.54 ± 20.33 weeks in group 2. The epithelial defects all resolved. Signs and symptoms improved in both groups. In group 2, the defect recurred after the suspension of therapy in 2 (18%) patients; in group 1, no defects recurred.Conclusions. Autologous serum eye drops effectively stabilize and improve signs and symptoms in eyes previously treated with conventional therapy.

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Effect of Autologous Serum Eye Drops in Patients with Sjögren Syndrome-related Dry Eye: Clinical and In Vivo Confocal Microscopy Evaluation of the Ocular Surface

In Vivo ◽

10.21873/invivo.11016 ◽

2016 ◽

Vol 30 (6) ◽

pp. 931-938 ◽

Cited By ~ 15

Author(s):

FRANCESCO SEMERARO ◽

ELIANA FORBICE ◽

GIUSEPPE NASCIMBENI ◽

MARCO TAGLIETTI ◽

VITO ROMANO ◽

...

Keyword(s):

Confocal Microscopy ◽

Dry Eye ◽

Ocular Surface ◽

Sjögren Syndrome ◽

Autologous Serum ◽

Sjogren Syndrome ◽

Eye Drops ◽

In Vivo Confocal Microscopy ◽

Serum Eye Drops

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Autologous serum eye drops for ocular surface disorders- a clinical study

International Journal of Medical Research and Review ◽

10.17511/ijmrr.2015.i4.074 ◽

2015 ◽

Vol 3 (4) ◽

pp. 394-399

Author(s):

Dr. Vijaya Sahu ◽

◽

Dr. Nidhi Pandey ◽

Dr. A K Chandrakar ◽

Dr. M.L. Garg ◽

...

Keyword(s):

Clinical Study ◽

Ocular Surface ◽

Autologous Serum ◽

Eye Drops ◽

Serum Eye Drops

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Applications of Autologous Serum Eye Drops in Aniridic Keratopathy

European Ophthalmic Review ◽

10.17925/eor.2009.03.01.51 ◽

2009 ◽

Vol 03 (01) ◽

pp. 51 ◽

Cited By ~ 2

Author(s):

José Santiago López-García ◽

Keyword(s):

Growth Factors ◽

Tear Film ◽

Biomechanical Properties ◽

Autologous Serum ◽

Eye Drops ◽

Impression Cytology ◽

Film Instability ◽

Congenital Aniridia ◽

Corneal Cells ◽

Serum Eye Drops

Aniridia is an uncommon congenital bilateral disease that involves the cornea, anterior chamber, lens, retina and optic nerve. Corneal changes in aniridic keratopathy include recurrent erosions of corneal epithelium, tear film instability, chronic pain, corneal vascularisation, dry eye, progressive corneal opacification and blindness. The autologous serum contains a variety of growth factors, vitamins and immunoglobulins, some of which are in higher concentrations than in natural tears. The growth factors and protein found in the serum may help the proliferation, migration and adhesion of epithelial corneal cells. They are by nature non-allergenic and their biochemical and biomechanical properties are similar to those of normal tears. The ocular surface, including corneal impression cytology and tear film evaluation, of patients with congenital aniridia was studied prior to and after treatment with autologous serum eye drops.

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A Case-Control Study on the Oxidative Balance of 50% Autologous Serum Eye Drops

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/9780193 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Patrícia Ioschpe Gus ◽

Diane Marinho ◽

Samira Zelanis ◽

Adriane Belló-Klein ◽

Claudete Locatelli ◽

...

Keyword(s):

Ocular Surface ◽

Case Control Study ◽

Case Control ◽

Demographic Characteristics ◽

Ocular Surface Disease ◽

Autologous Serum ◽

Eye Drops ◽

Healthy Controls ◽

Oxidative Balance ◽

Control Study

Importance.Autologous serum (AS) eye drops are recommended for severe dry eye in patients with ocular surface disease. No description of the antioxidant balance of AS eye drops has been reported in the literature.Objective.This study sought to evaluate the total reactive antioxidant potential (TRAP) and concentration of reactive oxygen species (ROS) in samples of 50% AS eye drops and their correlations with the demographic characteristics and lifestyle habits of patients with ocular surface disease and healthy controls.Design.This was a case-control study with a 3-month follow-up period.Participants. 16 patients with severe dry eye disease of different etiologies and 17 healthy controls matched by age, gender, and race were included.Results.TRAP and ROS were detected at all evaluated times. There were no differences in the mean ROS (p=0.429) or TRAP (p=0.475) levels between cases and controls. No statistically significant differences in the concentrations of ROS or TRAPs were found at 0, 15, or 30 days (pfor ROS = 0.087 andpfor TRAP = 0.93). Neither the demographic characteristics nor the lifestyle habits were correlated with the oxidative balance of the 50% AS eye drops.Conclusions and Relevance.Both fresh and frozen 50% AS eye drops present antioxidant capacities and ROS in an apparently stable balance. Moreover, patients with ocular surface disease and normal controls produce equivalent AS eye drops in terms of oxidative properties.

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