Stability of freeze-dried plasma rich in growth factors eye drops stored for 3 months at different temperature conditions

2020 ◽  
pp. 112067212091303 ◽  
Author(s):  
Eduardo Anitua ◽  
María de la Fuente ◽  
Francisco Muruzábal ◽  
Jesús Merayo-Lloves
Keyword(s):  
Growth Factors ◽  
Room Temperature ◽  
Storage Condition ◽  
Eye Drops ◽  
Temperature Conditions ◽  
Healthy Donors ◽  
Freeze Dried ◽  
And Storage ◽  
Blood Centrifugation ◽  
Human Keratocytes

Purpose: The purpose of this study was to analyze the biological content and activity of freeze-dried plasma rich in growth factors eye drops after their storage at 4°C and at room temperature for 3 months with respect to fresh samples (time 0). Methods: Plasma rich in growth factors was obtained after blood centrifugation from three healthy donors. After platelet activation, the obtained plasma rich in growth factors eye drops were lyophilized alone or in combination with lyoprotectant (trehalose), then they were stored for 3 months at room temperature or at 4°C. Several growth factors were analyzed at each storage time and condition. Furthermore, the proliferative and migratory potential of freeze-dried plasma rich in growth factors eye drops kept for 3 months at different temperature conditions was evaluated on primary human keratocytes. Results: The different growth factors analyzed maintained their levels at each time and storage condition. Freeze-dried plasma rich in growth factors eye drops stored at room temperature or 4°C for 3 months showed no significant differences on the proliferative activity of keratocytes in comparison with fresh samples. However, the number of migratory human keratocytes increased significantly after treatment with lyophilized plasma rich in growth factors eye drops kept for 3 months compared to those obtained at time 0. No significant differences were observed between the freeze-dried plasma rich in growth factors eye drops whether mixed or not with lyoprotectant. Conclusion: Freeze-dried plasma rich in growth factors eye drops preserve the main growth factors and their biological activity after storage at room temperature or 4°C for up to 3 months. Lyophilized plasma rich in growth factors eye drops conserve their biological features even without the use of lyoprotectants for at least 3 months.

scholarly journals The influence of selected preservation methods and storage time on the content of antioxidants in blackthorn (Prunus spinosa L.) fruits

2019 ◽  
Vol 74 (1) ◽  
pp. 53-62
Author(s):  
KATARZYNA OLESIŃSKA ◽  
DANUTA SUGIER ◽  
ŁUKASZ SĘCZYK
Keyword(s):  
Room Temperature ◽  
Storage Time ◽  
Antioxidant Properties ◽  
Iron Ions ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
Dried Fruits ◽  
Prunus Spinosa ◽  
Freeze Dried ◽  
And Storage

The aim of the study was to determine the influence of selected methods of preservation and storage time on the content of antioxidants in blackthorn fruit (Prunus spinosa L.). Preserved fruits were stored for 13 and 26 weeks without light at room temperature (freeze-dried and convection dried) and at –20°C (frozen samples). It was shown that both the method of preservation and the storage time had significant influence on the content of polyphenols (anthocyanins, flavonoids, total polyphenols) and antioxidant properties (Fe3+ reduction strength) of blackthorn fruit. As the storage time progressed, the anthocyanins content decreased in all preservation variants. In the case of frozen fruit after 26 weeks of storage, the content of flavonoids (by 12.5%), total polyphenols (by 48%) and the ability to reduce the iron ions were increased (by 55%). In turn, in the dried fruits after this period, the anthocyanins content, the polyphenol content and reduction strength were reduced. The highest values of the parameters evaluated after the assumed storage time has been demonstrated in lyophilizates.

scholarly journals Development and Optimization of Freeze-Dried Eye Drops Derived From Plasma Rich in Growth Factors Technology

2020 ◽  
Vol 9 (7) ◽  
pp. 35
Author(s):  
Eduardo Anitua ◽  
María de la Fuente ◽  
Ignacio Alcalde ◽  
Cristina Sanchez ◽  
Jesús Merayo-Lloves ◽  
...  
Keyword(s):  
Growth Factors ◽  
Eye Drops ◽  
Freeze Dried

scholarly journals Protective Effects of Tropical Fruit Processing Coproducts on Probiotic Lactobacillus Strains during Freeze-Drying and Storage

2020 ◽  
Vol 8 (1) ◽  
pp. 96 ◽  
Author(s):  
Caroliny Mesquita Araújo ◽  
Karoliny Brito Sampaio ◽  
Francisca Nayara Dantas Duarte Menezes ◽  
Erika Tayse da Cruz Almeida ◽  
Marcos dos Santos Lima ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Freeze Drying ◽  
Room Temperature ◽  
Membrane Integrity ◽  
Added Value ◽  
Protective Effects ◽  
Tropical Fruit ◽  
Freeze Dried ◽  
Fruit Processing ◽  
And Storage

This study evaluated the protective effects of coproducts from agroindustrial processing of the tropical fruits acerola (Malpighia glabra L., ACE), cashew (Anacardium occidentale L., CAS), and guava (Psidium guayaba L., GUA) on the probiotics Lactobacillus paracasei L-10, Lactobacillus casei L-26, and Lactobacillus acidophilus LA-05 during freeze-drying and storage. The occurrence of damage to membrane integrity, membrane potential, and efflux activity of Lactobacillus cells after freeze-drying was evaluated by flow cytometry, and viable counts were measured immediately after freeze-drying and during 90 days of storage under refrigerated or room temperature conditions. Probiotic strains freeze-dried without substrate had the overall highest count reductions (0.5 ± 0.1 to 2.9 ± 0.3 log cycles) after freeze-drying. Probiotics freeze-dried with fruit processing coproducts had small cell subpopulations with damaged efflux activity and membrane potential. Average counts of probiotics freeze-dried with ACE, CAS, or GUA after 90 days of storage under refrigerated or room temperature were in the range of 4.2 ± 0.1 to 5.3 ± 0.2 and 2.6 ± 0.3 to 4.9 ± 0.2 log CFU/g, respectively, which were higher than those observed for strains freeze-dried without substrate. The greatest protective effects on freeze-dried probiotics were overall presented by ACE. These results revealed that ACE, CAS, and GUA can exert protective effects and increase the stability of probiotic lactobacilli during freeze-drying and storage, in addition to supporting a possible added-value destination for these agroindustrial coproducts as vehicles for probiotics and for the development of novel functional foods.

The Lucerne Protocol for the Production of Autologous Serum Eyedrops

2021 ◽  
Vol 238 (04) ◽  
pp. 346-348
Author(s):  
Frantisek Sanak ◽  
Philipp Baenninger ◽  
Claude Kaufmann ◽  
Katja Iselin ◽  
Lucas Bachmann ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Room Temperature ◽  
Transforming Growth Factor ◽  
Serum Levels ◽  
Autologous Serum ◽  
Vertical Position ◽  
Eye Drops ◽  
Significant Difference ◽  
Serum Eye Drops

Abstract Background There are a variety of protocols for manufacturing autologous serum (AS) eye drops. The Lucerne protocol for the production of AS eye drops uses a slightly reduced gravitational (g)-force and time for the centrifugation process (2500 × g for 10 minutes), compared to previously published optimised protocols, to obtain high levels of epitheliotropic growth factors (3000 × g for 15 minutes). The goal of this study was to compare the concentrations of growth factors, albumin and lysozyme in autologous serum eye drops manufactured with these protocols. Material and Methods Blood from 5 healthy volunteers was placed in plastic tubes without an anticoagulant. Tubes from each donor were left in a vertical position for 2 hours at room temperature to facilitate coagulation, followed by centrifugation at either 2500 × g for 10 minutes or at 3000 × g for 15 minutes at room temperature. The serum levels of beta nerve growth factor (β-NGF), transforming growth factor β1 (TGF-β1), epidermal growth factor (EGF), hepatocyte growth factor (HGF), platelet-derived growth factor BB (PDGF-BB) and vascular endothelial growth factor A (VEGF-A) were measured in triplicate with a multi-analyte Simple Plex platform. The Simple Plex cartridge allows each sample to be run in triplicate for each analyte and prevents any interaction between the antibody components for each biomarker. The serum level of albumin was measured by turbidimetric immunoassay Tina-quant and of lysozyme by single radial immunodiffusion assay. Results For all analytes, the reduced g-force and centrifugation time did not result in a significant difference in serum levels. Conclusions The Lucerne protocol for the production of autologous serum eye drops with reduced g-force and a shorter centrifugation time does not affect the concentrations of the main epitheliotropic growth factors, albumin and lysozyme, in AS eye drops.

scholarly journals Rambutan seed germination: temperature and storage

2018 ◽  
Vol 8 (2) ◽  
pp. 383-388
Author(s):  
Renata Aparecida de Andrade ◽  
Amanda Garcia Bagatim ◽  
Guilherme Nacata
Keyword(s):  
Room Temperature ◽  
Storage Condition ◽  
Storage Management ◽  
Significant Difference ◽  
Germination Temperature ◽  
Two Stages ◽  
And Storage ◽  
Plastic Containers ◽  
Seeds Germination ◽  
Temperature Storage

The objective of this study was to verify if the germination temperature, storage condition and period could influence rambutan seeds germination. The research were realized in two stages, corresponding to rambutan seeds storage management: after extracted from the fruits or kept in the fruits, until sowing. Storage condition were also evaluated: low temperature (10oC) and room temperature, being realized the sowing at 0 (control), 3, 6, 9 and 12 days after storage, at different germination temperatures: 20, 25, 30, 35 and 40°C. The seeds were placed in plastic containers with commercial substrate with pine, using four repetitions with 10 seeds for each. The percentage and period of germination were assessed. The experimental design was completely randomized and the data was evaluated by variance analysis, applying Tukey´s test at 5% of significance for means with significant difference. According to the results, it is possible to conclude that the best temperature for rambutan germination is 25°C and the storage can be realized with the seeds inside the fruit until the time of sowing, in up tol six days.

scholarly journals Proteomic Characterization of Plasma Rich in Growth Factors and Undiluted Autologous Serum

2021 ◽  
Vol 22 (22) ◽  
pp. 12176
Author(s):  
Eduardo Anitua ◽  
Francisco Muruzabal ◽  
Ander Pino ◽  
Roberto Prado ◽  
Mikel Azkargorta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Growth Factors ◽  
Ocular Surface ◽  
Autologous Serum ◽  
Eye Drops ◽  
Differentially Expressed Proteins ◽  
Protein Profiles ◽  
Differential Protein ◽  
Healthy Donors

Over the last three decades, there has been special interest in developing drugs that mimic the characteristics of natural tears for use it in the treatment of several ocular surface disorders. Interestingly, the composition of blood plasma is very similar to tears. Therefore, different blood-derived products like autologous serum (AS) and plasma rich in growth factors (PRGF) have been developed for the treatment of diverse ocular pathologies. However, scarce studies have been carried out to analyze the differences between both types of blood-derived products. In the present study, blood from three healthy donors was drawn and processed to obtain AS and PRGF eye drops. Then, human corneal stromal keratocytes (HK) were treated with PRGF or undiluted AS. Proteomic analysis was carried out to analyze and characterize the differential protein profiles between PRGF and AS, and the differentially expressed proteins in HK cells after PRGF and AS treatment. The results obtained in the present study show that undiluted AS induces the activation of different pathways related to an inflammatory, angiogenic, oxidative stress and scarring response in HK cells regarding PRGF. These results suggest that PRGF could be a better alternative than AS for the treatment of ocular surface disorders.

scholarly journals Stability of Eleutherine americana (L.) Merr. Extract as Lipstick Colorants as the Change of Temperature, Time, Storage Condition and the Presence of Oxidator

2018 ◽  
Vol 5 (1) ◽  
pp. 8 ◽  
Author(s):  
Dewi Mayasari ◽  
Taofik Rusdiana ◽  
Yulien Ratu Kania ◽  
Marline Abdassah
Keyword(s):  
Room Temperature ◽  
Storage Condition ◽  
Stability Test ◽  
C 50 ◽  
The Stability ◽  
And Storage ◽  
Temperature Time ◽  
Color Pigment ◽  
Eleutherine Americana ◽  
Temperature Storage

Bawang Tiwai (Eleutherine americana (L.) Merr bulbus. is a typical plant from Kutai tribes which commonly used as an anti-bacterial and food coloring. The stability test is performed to determine the stability of the extract. Stability test was conducted in two solvents are distilled water and ethanol include the effects of temperature (25°C, 50°C, 80°C), pH (3, 5, 7), an oxidant for 6 hours, conditions of storage at room temperature and the temperature of the refrigerator for 24 hours and storage for 2 weeks. The absorbance of extract in ethanol and aquadest was decreasing due to the change of temperature, time, the presence of oxidator but due to pH changes, the absorbance of extract was increasing. Meanwhile, on the storage condition, showed the highest absorbance on dark, and room temperature storage compared to other condition.Key words: Eleutherine americana (L.) Merr bulbus, stability, color pigment

scholarly journals Impact of Chemicals and Storage Conditions on Post-harvest Quality of Litchi (Litchi chinensis Sonn.) cv. China

2021 ◽  
pp. 17-25
Author(s):  
Mary Sumi ◽  
Animesh Sarkar ◽  
C. S. Maiti ◽  
Akali Sema ◽  
S. P. Kanaujia
Keyword(s):  
Room Temperature ◽  
Calcium Nitrate ◽  
Storage Condition ◽  
Storage Conditions ◽  
Hot Water ◽  
Soluble Solids ◽  
Anthocyanin Content ◽  
Litchi Fruit ◽  
And Storage

The efficacy of chemical dips and different storage condition, alone and in combination were imposed in freshly harvested litchi fruit to investigate the changes of bio-chemical composition of fruits at regular interval for a certain period of storage. The pre-cooled litchi fruits after disinfectant were dipped for 60 seconds in lecithin (0.5% & 1%), Hot water, HCl (3%) with hot water, CaCl2 (1% & 2%), CaSO4 (1% & 2%), wax emulsion (5% & 10%), CaNO3 (0.5% & 1%), Chitosan (1% & 2%) and Kaolin (1% & 2%). The treated fruits were stored at room temperature as well as at 4°C & 90% R.H and the quality attributes were assessed at 2, 4, 6 and 8 days interval. Calcium nitrate @ 1% under refrigerated conditions recorded maximum ascorbic acid (54.50 mg/100g pulp), fruit firmness (1.75 kg cm-2) and the lowest acidity (0.42%). Total soluble solids (18.17°B), total sugar (11.27%) and polymeric colour retention with high anthocyanin content (48.94 mg/100g pulp) in peel was found in treated fruits with calcium chloride @1% at 4°C & 90% R.H even after 8 days of storage.

scholarly journals Hydrogel Encapsulation of Lactobacillus casei by Block Charge Modified Pectin and Improved Gastric and Storage Stability

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1337
Author(s):  
Qingshen Sun ◽  
Louise Wicker
Keyword(s):  
Targeted Delivery ◽  
Lactobacillus Casei ◽  
Room Temperature ◽  
In Vitro Release ◽  
Pectin Methylesterase ◽  
Citrus Pectin ◽  
Freeze Dried ◽  
And Storage ◽  
Vitro Release

Lactobacillus casei (L. casei W8) was encapsulated in pectin methylesterase (PME) charge modified pectin hydrogels; stability and in vitro release were evaluated under simulated gastrointestinal (GI) conditions. PME, 355 U/mL, de-esterified citrus pectin to 35% from 72% degree of esterification (DE). Pectin ζ-potential decreased to about −37 mV and molecular weight decreased from 177 kDa to 143 kDa during charge modification. More than 99% L. casei W8 were encapsulated in block charged, low methoxy pectin (35 mLMP) hydrogels by calcium ionotropic gelation. The integrity of the hydrogels was maintained under simulated GI conditions, and no release of L. casei W8 was observed. Microbial counts of encapsulated L. casei ranged from 6.94 log CFU/g to 10.89 log CFU/g and were 1.23 log CFU/g higher than for unencapsulated L. casei W8. The viability of encapsulated L. casei W8 in wet hydrogels remained the same for 2 weeks, but nearly all flora died after 4 weeks storage at 4 °C. However, freeze dried hydrogels of L. casei W8 were viable for 42 days at 4 °C and 14 days at room temperature. Charge modified pectin hydrogels are potentially good vehicles for colon-targeted delivery carrier for probiotics and longer stability of L. casei W8.

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